標題: Titlebook: Recombinant Protein Expression in Mammalian Cells; Methods and Protocol David L. Hacker Book 2024Latest edition The Editor(s) (if applicabl [打印本頁] 作者: Agoraphobia 時間: 2025-3-21 18:57
書目名稱Recombinant Protein Expression in Mammalian Cells影響因子(影響力)
書目名稱Recombinant Protein Expression in Mammalian Cells影響因子(影響力)學科排名
書目名稱Recombinant Protein Expression in Mammalian Cells網(wǎng)絡公開度
書目名稱Recombinant Protein Expression in Mammalian Cells網(wǎng)絡公開度學科排名
書目名稱Recombinant Protein Expression in Mammalian Cells被引頻次
書目名稱Recombinant Protein Expression in Mammalian Cells被引頻次學科排名
書目名稱Recombinant Protein Expression in Mammalian Cells年度引用
書目名稱Recombinant Protein Expression in Mammalian Cells年度引用學科排名
書目名稱Recombinant Protein Expression in Mammalian Cells讀者反饋
書目名稱Recombinant Protein Expression in Mammalian Cells讀者反饋學科排名
作者: 你敢命令 時間: 2025-3-21 22:40 作者: nauseate 時間: 2025-3-22 02:19
Tania F. Custódio,Gabriela Guédez,Christian L?wy of problems. The first application addressed the problem of troubleshooting and repairing diesel electric locomotives in railroad .. In such shops, railroad maintenance personnel had to detect and repair a large variety of faults that partially disabled a diesel electric locomotive..The expert sys作者: 縮減了 時間: 2025-3-22 06:52
Julia Flock,Yexin Xie,Régis Lemaitre,Karine Lapouge,Kim Remansy of problems. The first application addressed the problem of troubleshooting and repairing diesel electric locomotives in railroad .. In such shops, railroad maintenance personnel had to detect and repair a large variety of faults that partially disabled a diesel electric locomotive..The expert sys作者: 影響帶來 時間: 2025-3-22 09:25 作者: 車床 時間: 2025-3-22 14:42
Jenny Gunnarsson,David I. Fisher,Robert G. Rothent, and of the efforts directed towards the development and application of the related expert systems. The above mentioned activities are mostly related to pressurized nuclear and non-nuclear components. A large part of the research work has been performed within the scope of the Reactor Safety Res作者: 啞劇 時間: 2025-3-22 19:09 作者: Licentious 時間: 2025-3-23 00:45 作者: 延期 時間: 2025-3-23 02:31
David L. HackerIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Individual 時間: 2025-3-23 07:52 作者: 哀悼 時間: 2025-3-23 13:25 作者: GUILT 時間: 2025-3-23 14:35
Transient Co-expression of Membrane Protein Complexes in Mammalian Cells,ystem. The protocol can be applied for small-scale screening of any membrane protein alone or co-expressed with interacting partners followed by large-scale production and purification of a potential membrane protein complex.作者: 神圣不可 時間: 2025-3-23 19:34
Recombinant Protein Production from Stable CHO Cell Pools,media and polyethylenimine (PEI) as the transfection reagent. As a specific example of how this protocol can be applied, the production and downstream purification of recombinant His-tagged trimeric SARS-CoV-2 spike protein ectodomain (SmT1) are described.作者: Vasoconstrictor 時間: 2025-3-23 23:05 作者: 單片眼鏡 時間: 2025-3-24 05:51
CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells,ecombinant protein productivity. In this chapter, we present a protocol for targeting miRNAs in CHO cells using CRISPR/Cas9 and the analysis of the resulting phenotype, using miR-27 as an example. We show that it is possible to target miRNAs in CHO cells and achieved ≥80% targeting efficiency. Indel作者: agnostic 時間: 2025-3-24 09:37
High-Throughput Protein Expression Screening of Cell-Surface Protein Ectodomains,ioritize well-expressing construct for large-scale production. By employing this method, one can efficiently detect clones with low expression levels, streamlining the process and saving valuable time in identifying optimal candidates for further study.作者: 戲服 時間: 2025-3-24 14:28
ding to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami作者: 小步走路 時間: 2025-3-24 16:00
Yashas Rajendrading to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami作者: 清楚說話 時間: 2025-3-24 19:21 作者: expository 時間: 2025-3-25 00:08
Julia Flock,Yexin Xie,Régis Lemaitre,Karine Lapouge,Kim Remans Field Service Engineer. The inference engine used a mixed-mode configuration, capable of running in either the forward or backward mode. The Help System provided the operator with assistance by displaying textual information, CAD diagrams or repair sequences from a video disk. The rules were writte作者: 延期 時間: 2025-3-25 07:05 作者: 聰明 時間: 2025-3-25 09:00
Jenny Gunnarsson,David I. Fisher,Robert G. Rothding to the real ones in terms of internal pressure, temperature, corrosion, etc., which has been of decisive importance for the transferability of the test results to real components. In addition, a series of complementary issues has been investigated (material science aspects, nondestructive exami作者: habitat 時間: 2025-3-25 13:39
1064-3745 ls, Second Edition. serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins..978-1-0716-3880-4978-1-0716-3878-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 龍卷風 時間: 2025-3-25 18:45 作者: Extort 時間: 2025-3-25 20:13 作者: 鉗子 時間: 2025-3-26 03:13 作者: etiquette 時間: 2025-3-26 07:09 作者: conduct 時間: 2025-3-26 08:41 作者: 先驅(qū) 時間: 2025-3-26 14:28
Qiong Wang,Lateef Aliyu,Cheng-Yu Chung,Julian N. Rosenberg,Geng Yu,Michael J. Betenbaugh作者: Essential 時間: 2025-3-26 18:43
Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CH作者: 步兵 時間: 2025-3-26 23:16
Book 2024Latest editionbleshooting and avoiding known pitfalls.?.Authoritative and practical, .Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition. serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins..作者: 6Applepolish 時間: 2025-3-27 02:24
Expression of Bispecific Antibodies Using Cellular Display, methods are provided enabling the flow cytometric sorting of a cell population based on the quantity of cellular display or on the product quality (heterodimerization level of a bispecific antibody).作者: cortisol 時間: 2025-3-27 06:12 作者: Amnesty 時間: 2025-3-27 12:33 作者: 靈敏 時間: 2025-3-27 15:11 作者: GNAW 時間: 2025-3-27 18:39 作者: pancreas 時間: 2025-3-27 22:58 作者: 勤勞 時間: 2025-3-28 05:27 作者: 手榴彈 時間: 2025-3-28 07:15 作者: genuine 時間: 2025-3-28 12:01 作者: 獨白 時間: 2025-3-28 18:25 作者: Rankle 時間: 2025-3-28 20:20
Recombinant CHO Cell Pool Generation Using PiggyBac Transposon System,re, we describe the generation of CHO cell pools using the piggyBac transposon system for mediating gene integration. The method describes the co-transfection of cells with the donor plasmid (coding for the gene of interest) and the helper plasmid (coding for the transposase) using polyethyleneimine作者: BOON 時間: 2025-3-29 01:14 作者: 道學氣 時間: 2025-3-29 05:50
Expression of Multispecific Antibodies,the increased complexity (e.g., the interface design and the presence of multiple binders), such molecules are generally more challenging to express and purify compared to standard monoclonal antibodies (mAbs). We describe here an optimized methodology to express and purify basic bispecific antibodi作者: Influx 時間: 2025-3-29 09:45 作者: Nefarious 時間: 2025-3-29 13:48 作者: Devastate 時間: 2025-3-29 19:07 作者: 匍匐前進 時間: 2025-3-29 21:25
Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cellove both the productivity of recombinant cell lines as well as the quality of therapeutic antibodies. Antibody glycosylation is a critical quality attribute for therapeutic biologics because the glycan patterns on the antibody fragment crystallizable (Fc) region can alter its clinical efficacy and s作者: 周興旺 時間: 2025-3-30 03:00
Development of Responsive Promoters and their Utilization for Stable CHO Sensor Cell Lines,f the desired molecule, it is important to monitor and adjust bioprocess parameters like oxygen concentration as well as osmolality. However, the observation of crucial cultivation parameters can be an elaborate procedure requiring lots of hands-on work. In addition, for emerging modeling approaches作者: 機密 時間: 2025-3-30 06:54
CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells,lly hundreds of target genes. In that regard, their utility has been demonstrated as a strategy to improve the cellular phenotypes important in the biomanufacturing of recombinant proteins. Common approaches to stably deplete miRNAs are the use of sponge decoy transcripts or shRNA inhibitors, both o作者: Venules 時間: 2025-3-30 12:01
High-Throughput Protein Expression Screening of Cell-Surface Protein Ectodomains,tion, and instability. Cell-surface receptor ectodomains are more amenable to large-scale production, but this requires designing and testing various truncation constructs. However, since each protein is unique, testing these constructs individually for many targets is a time-consuming process. In t作者: 殘廢的火焰 時間: 2025-3-30 15:43 作者: 仔細檢查 時間: 2025-3-30 19:03 作者: 凹槽 時間: 2025-3-30 23:04
Generation of Monoclonal Chinese Hamster Ovary Cell Lines Using DISPENCELL-S3,terization of an adequate cell sample for single-cell isolation, as well as the optimization of the DISPENCELL-S3 dispensing parameters are described. Monoclonal outgrowth assessment and the use of the recorded impedance signal as evidence of clonality are also outlined.作者: 極少 時間: 2025-3-31 01:44
The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Productitep-by-step description of the protocols we use for the generation of the recombinant BacMam viruses, the transduction of mammalian cell cultures, and optimization of the protein production conditions through small-scale expression and purification tests.作者: 陪審團每個人 時間: 2025-3-31 09:00 作者: surmount 時間: 2025-3-31 12:12 作者: 使激動 時間: 2025-3-31 17:09
,A Versatile Method for Inducible Protein Production in 293 Cells Using?the PiggyBac Transposon Systmethodology is based on the . transposon system and enables?the?inducible production of the protein of interest. Finally,?this methodology can easily be used in conventional laboratory cell culture settings without requiring specialized devices.作者: 合法 時間: 2025-3-31 19:35 作者: ARENA 時間: 2025-3-31 23:16
Expression of Multispecific Antibodies,es using the BEAT. interface. This interface allows to generate antibodies with very high levels of heterodimer product (reported titers exceed 10?g/L) and comes with a built-in purification strategy allowing removal of residual levels of undesired product-related impurities (e.g., homodimers and half molecules).作者: compel 時間: 2025-4-1 03:47
Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Moribe a detailed protocol to identify integration sites with epigenetically preferable properties by chromatin immunoprecipitation sequencing and use them for stable and strong gene expression by applying CRISPR/Cas9. Furthermore, the examination of the integration sites with focus on Cas9-targeted sequencing with nanopores is described.作者: thrombosis 時間: 2025-4-1 08:40 作者: BIAS 時間: 2025-4-1 11:23
Book 2024Latest editionr transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifyin作者: erythema 時間: 2025-4-1 15:21 作者: 表狀態(tài) 時間: 2025-4-1 19:16
Book 2006der the German Copyright Law. Springer is a part of Springer Science+Business Media springeronline. com ? Springer-Verlag Berlin Heidelberg 2006 Printed in Germany The use of general descriptive names, registered names, trademarks, etc. in this pub- cation does not imply, even in the absence of a sp作者: inspired 時間: 2025-4-2 01:58
2520-8799 en auditiven und filmischen Arbeiten. Dadurch entsteht ein Gesamtbild eines in seinen verschiedenen medialen Ausformungen motivisch und thematisch intensiv verwobenen Werks – von intertextuellen Aspekten über die enorme Bedeutung von Musik bis zur Verknüpfung mit Fotografie..978-3-662-62930-7Series ISSN 2520-8799 Series E-ISSN 2520-8802 作者: 完成才會征服 時間: 2025-4-2 05:46
