標題: Titlebook: Receptor Signal Transduction Protocols; R. A. J. Challiss Book 1997 Springer Science+Business Media New York 1997 [打印本頁] 作者: 延展 時間: 2025-3-21 20:01
書目名稱Receptor Signal Transduction Protocols影響因子(影響力)
書目名稱Receptor Signal Transduction Protocols影響因子(影響力)學科排名
書目名稱Receptor Signal Transduction Protocols網(wǎng)絡(luò)公開度
書目名稱Receptor Signal Transduction Protocols網(wǎng)絡(luò)公開度學科排名
書目名稱Receptor Signal Transduction Protocols被引頻次
書目名稱Receptor Signal Transduction Protocols被引頻次學科排名
書目名稱Receptor Signal Transduction Protocols年度引用
書目名稱Receptor Signal Transduction Protocols年度引用學科排名
書目名稱Receptor Signal Transduction Protocols讀者反饋
書目名稱Receptor Signal Transduction Protocols讀者反饋學科排名
作者: AUGER 時間: 2025-3-21 21:21
1064-3745 bi- ogy and has attracted the attentions of almost every traditional discipline within the biological sciences. At the heart of these divergent endeavors are common methods that can aid biochemists, physiologists, and pharmacologists in ta- ling the specific questions addressed by their research. I作者: Angiogenesis 時間: 2025-3-22 03:36
Radioligand-Binding Methods for Membrane Preparations and Intact Cellsthermore, the technique can be adapted to study the association and dissociation kinetics of llgand binding (.,.), as well as complex allosteric mteractlons between ligands (.) or between receptors and effector molecules, such as guanine nucleotide-binding proteins (G proteins) (.,.).作者: 情感 時間: 2025-3-22 06:27 作者: hyperuricemia 時間: 2025-3-22 10:04
Covalent Modification of G Proteins by Affinity Labelingexist for stringent coupling of a receptor to a single species of a heterotrimer composed of a defined α-, β-, and γ-subunit, as well as promrscuous interaction with a wide variety of different G protein subunits (.)作者: 痛打 時間: 2025-3-22 16:15
Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer toom the Gβγ dimer. Both activated Gα-GTP and free Gβγ have the capability of interacting with different effecters, e.g., adenylyl cyclases, phospholipases Cβ (PLCβ), and ion channels. It is of growing interest to establish whether receptors may be able to select among individual α-, β-, and γ-subunits for coupling to specific effector systems.作者: disciplined 時間: 2025-3-22 18:11
Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channelses. Subumt stoichiometry, mode of assembly, and the relative expression levels of individual cDNA vector constructs also have to be taken into account. The latter still poses an insurmountable problem, because no accurate predictions can be made.作者: Intellectual 時間: 2025-3-22 21:25
lmmunocytochemical Methods for Investigating Receptor Localizationntal manipulation of cellular function, has provided powerful Insights not only into the structural aspects of receptor localization and topology, but also into dynamic processes of receptor biology, including coupling to effecters, intracellular trafficking, ontogenesls, and disease.作者: FLEET 時間: 2025-3-23 03:10 作者: arcane 時間: 2025-3-23 09:16 作者: MOCK 時間: 2025-3-23 12:04
Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Bindinghe most part been limited to study of the structure-activity relationships (SAR) of agonists and antagonists at the individual receptors. For a particular receptor, these SAR data were obtained via synthesis of series of chemically modified hgands, followed by the determination of their binding acti作者: 呼吸 時間: 2025-3-23 14:37
Approaches to the Stable Transfection of G Protein-Coupled Receptorsnction. The use of stably transfected cell lines enables a receptor to be studied in the absence of other related receptor subtypes. With this technique, molecular determinants of ligand binding can be identified; signal transduction pathways of a particular receptor can be delineated; interactions 作者: 殺蟲劑 時間: 2025-3-23 21:48
Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channelsde variety of proteins (.–.). Transient transfections are especially practical for the expression of recombinant multisubunit complexes because their expression needs the prior knowledge and selection of which of a set of subunits are necessary to form functional heteromultrmers with distinct featur作者: deficiency 時間: 2025-3-24 01:14
The Generation of Receptor-Selective Antibodieshan pharmacological probes. They are often the only probes available that can discern proteins with similar structural and pharmacological properties, such as distinct isoforms or splice-variants of a receptor family. Furthermore, antibodies may be utilized to determine a wide range of a receptor’ c作者: 無聊點好 時間: 2025-3-24 03:33 作者: Harrowing 時間: 2025-3-24 09:34
lmmunocytochemical Methods for Investigating Receptor Localizationhe availability of immunochemical probes to simultaneously visualize cellular ultrastructure, such as intracellular organelles, cytoskeleton, proteins involved in signal transduction, and markers of cellular differentiation. The application of immunocytochemical methods, in conjunction with experime作者: 暗諷 時間: 2025-3-24 12:43 作者: exceed 時間: 2025-3-24 17:43 作者: 不法行為 時間: 2025-3-24 22:49
Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Couplingoteins with altered structures that can be studied for changes in biochemical properties. The most common way to do structure/function studies is by site-directed mutagenesis, in which a particular residue in a protein is selected and systematically altered. Because of the labor-intensive nature of 作者: 令人發(fā)膩 時間: 2025-3-25 02:57 作者: 百靈鳥 時間: 2025-3-25 07:07
Covalent Modification of G Proteins by Affinity Labeling identified a large array of closely related receptors (R), G protein subunits (G), and effecters (E). Currently, an important research goal is to understand the factors that, at each step of the cascade, link R to G to E, govern signal transfer, and signal integration following activation of a spec作者: 細節(jié) 時間: 2025-3-25 08:49 作者: AND 時間: 2025-3-25 11:53 作者: dapper 時間: 2025-3-25 18:56
Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primarheir inaccessibility, but also by the lack of specific tools. The problem is particularly acute when the effect of interest must be measured at the single cell level, for example, receptor modulation of ion channels. Assignment of different G protein subunits to roles in ion channel modulation by re作者: 離開可分裂 時間: 2025-3-25 21:05
Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylationcoupled receptor subtypes that have been demonstrated to undergo agonist-dependent phosphorylation (.). In fact, of the receptor subtypes studied, only three—namely the α.C4-adrenoceptor (.), β.-adrenoceptor (.), and the gonadotrophin-releasing hormone receptor (.—are thought not to undergo agonist-作者: Initiative 時間: 2025-3-26 02:45 作者: 粗鄙的人 時間: 2025-3-26 05:14
Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cellssequestered into a cell compartment to which hydrophilic ligands have only a limited access (.–.). Although receptor sequestration has frequently been documented, its physiological significance and underlying mechanism(s) remain obscure. This is largely the result of the routine use of intact cells 作者: 勤勉 時間: 2025-3-26 10:26 作者: Junction 時間: 2025-3-26 13:52
978-1-4899-4283-8Springer Science+Business Media New York 1997作者: PRE 時間: 2025-3-26 17:34
Receptor Signal Transduction Protocols978-1-59259-567-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Genetics 時間: 2025-3-26 21:00 作者: 名字的誤用 時間: 2025-3-27 01:37
Measurement of Agonist-Stimulated [35S]GTPγS Binding to Cell MembranesAbstract This chapter describes a functional assay that measures the increase in guanine nucleotide exchange at G proteins in cell membranes, resulting from agonist binding to G protein-coupled receptors (GPCRs), by monitoring the binding of a radiolabeled, hydrolysis-resistant analog of GTP, [.S]GTPγS, in the presence of unlabeled GDP.作者: 淘氣 時間: 2025-3-27 05:37
In Situ Hybridizationptors, including new subtypes and sub-subtypes of previously identified receptors. With the dramatic increase in the number of these receptors, the technique of . hybridization (ISH) has become an essential addition to the tools available for receptor research.作者: BINGE 時間: 2025-3-27 13:19 作者: faddish 時間: 2025-3-27 16:55 作者: guardianship 時間: 2025-3-27 18:07
Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cellssequestered into a cell compartment to which hydrophilic ligands have only a limited access (.–.). Although receptor sequestration has frequently been documented, its physiological significance and underlying mechanism(s) remain obscure. This is largely the result of the routine use of intact cells for the measurement of sequestration events.作者: 消散 時間: 2025-3-27 23:12
eine geeignete Ma?nahme. Der Einsatz niedrigviskoser Schmierstoffe sowie die Erh?hung der Leistungsdichte von Getrieben führen jedoch zu einem vermehrten Betrieb der Getriebeverzahnungen im Mischreibungsgebiet. Um einem zunehmenden Verschlei? entgegenzuwirken, kann der Einsatz von reibungs- und ver作者: 反話 時間: 2025-3-28 02:40
Mary Keen eine geeignete Ma?nahme. Der Einsatz niedrigviskoser Schmierstoffe sowie die Erh?hung der Leistungsdichte von Getrieben führen jedoch zu einem vermehrten Betrieb der Getriebeverzahnungen im Mischreibungsgebiet. Um einem zunehmenden Verschlei? entgegenzuwirken, kann der Einsatz von reibungs- und ver作者: 親密 時間: 2025-3-28 06:50
Mark E. Olah,Gary L. Stilesg finden? Zur Beantwortung dieser Frage wird ein technischer Vergleich von permanentmagneterregter und fremderregter Synchronmaschine sowie Asynchronmaschine durchgeführt, wofür eine Maschine des jeweiligen Maschinentyps mit einer Spitzenleistung von ca. 150?kW und einer maximalen Drehzahl von 14.00作者: 樂章 時間: 2025-3-28 13:41
Andrea Townsend-Nicholson als beim Verbrennungsmotor, bei dem üblicherweise die Dauer- der Spitzenleistung entspricht, unterscheiden sich diese beim Elektromotor je nach Kühlkonzept zum Teil erheblich. Je nach Applikation und Nutzungsprofil kann die Maschine w?hrend des Betriebs an ihre thermische Grenze gebracht werden und作者: 系列 時間: 2025-3-28 18:05 作者: 樣式 時間: 2025-3-28 20:49 作者: Biguanides 時間: 2025-3-29 01:54
Judith C. W. Mak,Peter J. Barneserungen erfüllen. Insbesondere die Entwicklung moderner Ottomotoren für kommende Abgasgesetze wie EU7 wird ma?geblich von den RDE-Grenzwerten beeinflusst. Um dieser Herausforderung zu begegnen, wird der Einsatz virtueller Entwicklungsmethoden immer wichtiger, um die Entwicklungszeit zu verkürzen und作者: Affluence 時間: 2025-3-29 05:04 作者: 吞下 時間: 2025-3-29 07:54 作者: 不安 時間: 2025-3-29 13:49 作者: 細絲 時間: 2025-3-29 16:44 作者: 救護車 時間: 2025-3-29 23:29
Ethan S. Burstein,Tracy A. Spalding,Mark R. Branntemen erweitert. Der Wechsel von der synthetischen Testumgebung eines Prüfstands in Richtung der Stra?e, als komplexes, unvorhersehbares Testgel?nde, stellt eine gro?e Herausforderung dar und erh?ht den Kalibrieraufwand für die Fahrzeughersteller erheblich. Methodische Anpassungen an bestehenden Ent作者: 總 時間: 2025-3-30 00:37
Ian Mullaney,Graeme Milliganlopment process phase or during troubleshooting. Basically, we can distinguish the pure torsional and transient multi-body approaches (MBD), rigid or flexible structures and the level of detailing..Another important aspect is the type of operating conditions. Typically, different operating condition作者: 使尷尬 時間: 2025-3-30 05:09 作者: FLINT 時間: 2025-3-30 10:45
Stephen R. Ikedaetrien werden zur Qualit?tssicherung u.?a. anhand relevanter Bauteilmerkmale geprüft. Die Lageprüfung misst die Ist-Position eines Merkmals in einer r?umlichen Dimension. Informationen über die Ausrichtung und Geometrie des Brennraums k?nnen durch die eingesetzten Messverfahren nicht abgeleitet werd作者: 易發(fā)怒 時間: 2025-3-30 14:26 作者: 大量 時間: 2025-3-30 17:54 作者: 我要沮喪 時間: 2025-3-30 21:01 作者: Catheter 時間: 2025-3-31 04:48 作者: 嘴唇可修剪 時間: 2025-3-31 08:27
Approaches to the Stable Transfection of G Protein-Coupled Receptorswn, constant quantity of the transfected receptor is expressed for many generations. Both stably transfected pools and clonal cell lines can be studied. Stably transfected pools, or clonal cell lines, that express high levels of a transfected receptor facilitate ligand binding studies; cell lines ex作者: 叫喊 時間: 2025-3-31 11:07
Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation ises, and cGMP phosphodiesterase (.). The a-subunit of G protein has inherent high-affinity or low-K. GTPase activity, which hydrolyzes GTP on the a-subunit into GDP and inorganic phosphate (P,) (.). This turn-off reaction results in reassociation of GDP-bound a-subunit and βγ-subunits to end the act作者: Lyme-disease 時間: 2025-3-31 14:24 作者: 油氈 時間: 2025-3-31 18:59
Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons b in a given cell (e.g., as determined by mnnunohistochemistry) is not sufficient to allow unambiguous assignment of a response to a particular receptor subtype, since additional uncharacterized members of a receptor family may exist. Thus, assigning roles to, and characterizing signaling pathways of作者: 變形 時間: 2025-3-31 23:05
Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primar, and antibody against Gα. inhibited muscarinic and bradykinin receptor effects on the M-type K. current (.,.). However, this approach is seriously limited by the close sequence homologies between members of G protein a-subunit subfamilies (.), which results in the antibodies being unable to discrim作者: 狼群 時間: 2025-4-1 05:17 作者: instulate 時間: 2025-4-1 08:18
Book 1997ethodologies with new technologies at the cutting-edge of signal transduction research and as such I hope the present volume will complement and extend a previous excellent volume in this series edited by David Kendall and Stephen Hill (Methods Molecular Biology, vol. 41, Signal Transd- tion Protocols).作者: Halfhearted 時間: 2025-4-1 12:25 作者: 凌辱 時間: 2025-4-1 17:12
Mary Keeng zu erzeugen..Anhand ausgew?hlter TEHD-Simulationen mit verschiedenen DLC-Schichten wird gezeigt, welchen Einfluss eine Beschichtung von Ritzel und/oder Rad auf die Temperaturentwicklung, die Reibungsverluste und den Wirkungsgrad hat und welche thermo-physikalischen Eigenschaften der Schichten von 作者: Bricklayer 時間: 2025-4-1 20:44 作者: BIAS 時間: 2025-4-2 00:54
Andrea Townsend-Nicholsonung des Temperaturmodells zurückgeführt werden. Um die Genauigkeit des thermischen Netzwerks auch unter diesen Einflussgr??en zu optimieren, ist die Untersuchung von Ma?nahmen zur Abweichungsreduzierung notwendig. In diesem Beitrag wird ein thermisches Netzwerk niedriger Ordnung für elektrisch erreg
