標(biāo)題: Titlebook: Rab GTPases; Methods and Protocol Guangpu Li Book 2015 Springer Science+Business Media New York 2015 Endocytic Pathways.Eukaryotes.Exocytic [打印本頁] 作者: 指責(zé) 時間: 2025-3-21 18:08
書目名稱Rab GTPases影響因子(影響力)
書目名稱Rab GTPases影響因子(影響力)學(xué)科排名
書目名稱Rab GTPases網(wǎng)絡(luò)公開度
書目名稱Rab GTPases網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Rab GTPases被引頻次
書目名稱Rab GTPases被引頻次學(xué)科排名
書目名稱Rab GTPases年度引用
書目名稱Rab GTPases年度引用學(xué)科排名
書目名稱Rab GTPases讀者反饋
書目名稱Rab GTPases讀者反饋學(xué)科排名
作者: Encumber 時間: 2025-3-21 22:39 作者: 飲料 時間: 2025-3-22 03:48 作者: MIME 時間: 2025-3-22 06:09 作者: anus928 時間: 2025-3-22 10:48 作者: 空氣 時間: 2025-3-22 14:23 作者: HACK 時間: 2025-3-22 18:46 作者: Encapsulate 時間: 2025-3-22 22:43
Bioinformatic Approaches to Identifying and Classifying Rab Proteins,cularly challenging for standard annotation pipelines because they are similar to other small GTPases and form a large family with many paralogous subfamilies. Here, we describe a bioinformatic annotation pipeline specifically tailored to Rab GTPases. It proceeds in two steps: first, Rabs are distin作者: 交響樂 時間: 2025-3-23 02:21 作者: fallible 時間: 2025-3-23 06:46
High-Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins,unction of GTPases in diverse cellular processes. For the Rab GTPase family, which comprises at least 60 distinct proteins in humans, putative GAPs have been identified from both eukaryotic organisms and pathogenic bacteria. A major obstacle has involved identification of target substrates and deter作者: 首創(chuàng)精神 時間: 2025-3-23 11:44
Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts,ing particular Rabs as substrates for their cognate GTPase-activating proteins. However, in vitro activity does not always reflect substrate specificity in cells. This method describes a functional test of GAP activity in cells or extracts that takes into account the presence of other factors or con作者: 古董 時間: 2025-3-23 16:02
Analysis of the Interactions Between Rab GTPases and Class V Myosins,ell division. Humans have over 40 myosins that can be placed into 18 classes, the malfunctioning of a number of which can lead to disease. There are three members of the human class V myosin family, myosins Va, Vb, and Vc. People lacking functional myosin Va suffer from a rare autosomal recessive di作者: Postulate 時間: 2025-3-23 21:15 作者: Intervention 時間: 2025-3-24 02:01
Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11,from the .-Golgi network (TGN) and recycling endosomes to the plasma membrane. Rabin8 is a major guanine nucleotide exchange factor (GEF) for Rab8. It activates Rab8 by catalyzing its GDP release for subsequent GTP loading. However, how Rabin8 itself is activated in cells is unclear. Recently, it wa作者: CURB 時間: 2025-3-24 05:38
Ypt1 and TRAPP Interactions: Optimization of Multicolor Bimolecular Fluorescence Complementation inare stimulated by guanine-nucleotide exchange factors (GEFs). In yeast, Ypt1 regulates transport from the endoplasmic reticulum (ER) to two alternative pathways: secretion and autophagy. Ypt1 is activated by TRAPP, a modular multi-subunit GEF. Whereas TRAPP I activates Ypt1 to mediate transport thro作者: 易于出錯 時間: 2025-3-24 08:49
Identifying a Rab Effector on the Macroautophagy Pathway,, and the macroautophagy pathway. To identify effectors on the macroautophagy pathway, known autophagy-related genes (Atg genes) required for macroautophagy were tagged with GFP and screened for mislocalization in the .-. mutant. At the pre-autophagosomal structure (PAS), the localization of the ser作者: CRUMB 時間: 2025-3-24 13:57 作者: Albinism 時間: 2025-3-24 15:02
Small GTPases in Acrosomal Exocytosis,ptor (SNARE) and Rab superfamilies are members of this machinery. Rab proteins are small GTPases that organize membrane microdomains on organelles by recruiting specific effectors that strongly influence the movement, fusion and fission dynamics of intracellular compartments. Rab3 and Rab27 are the 作者: CORE 時間: 2025-3-24 19:23
Rab Antibody Characterization: Comparison of Rab14 Antibodies,ll junctions to the plasma membrane. It localizes predominantly to endocytic membranes with a pool also found on .-Golgi network (TGN) membranes, and is most closely related to the Rab11 subfamily of GTPases. Certain intracellular bacteria such as ., ., and . utilize Rab14 to promote their maturatio作者: 露天歷史劇 時間: 2025-3-25 00:00
Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin,e the cell. However, it was difficult to distinguish GLUT4 storage vesicles (GSVs) from other intracellular compartments containing GLUT4 in live cells. Here, we describe the use of IRAP-pHluorin and total internal reflection fluorescence (TIRF) microscopy to selectively visualize GSVs and Rab prote作者: OMIT 時間: 2025-3-25 07:15
3D Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics During Apical Lumen Formation and distribution within cells. Although this technique can also be applied to polarized epithelial cells that form three-dimensional (3D) cysts when grown in a Matrigel matrix suspension, there are still significant limitations in imaging cells fixed at a particular point in time. Here, we describe作者: NIB 時間: 2025-3-25 09:18 作者: CLAIM 時間: 2025-3-25 12:50
Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis in the vesicular trafficking machinery change cellular bioenergetics that can be directly measured in real time as Oxygen Consumption Rate, OCR (mitochondrial respiration) and Extracellular Acidification Rate, ECAR (glycolysis) by an extracellular flux analyzer (XF96, Seahorse Biosciences, MA). Ad作者: 填料 時間: 2025-3-25 16:18
Measurement of Rab35 Activity with the GTP-Rab35 Trapper RBD35,ve form and a GDP-bound inactive form. Although Rab35 has been shown to regulate various cellular processes, including cytokinesis, cell migration, and neurite outgrowth, its precise roles in these processes are not fully understood. Since a molecular tool that could be used to measure Rab35 activit作者: Inoperable 時間: 2025-3-25 21:25
,Analysis of Connecdenn 1–3 (DENN1A-C) GEF Activity for Rab35,r sites. Rab GTPases toggle between an inactive GDP-bound state and an active GTP-bound state. Activation of Rab GTPases requires guanine nucleotide exchange factors (GEFs) that interact with inactive GDP-bound Rabs and catalyze the removal of GDP, allowing GTP to bind. The largest single family of 作者: 是剝皮 時間: 2025-3-26 01:27 作者: affinity 時間: 2025-3-26 04:22
Book 2015 guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the作者: 有效 時間: 2025-3-26 08:57 作者: overhaul 時間: 2025-3-26 13:19
Measurement of Rab35 Activity with the GTP-Rab35 Trapper RBD35,bound active form of Rab35 and does not interact with any of the other 59 Rab proteins identified in humans and mice, RBD35 is a useful tool for measuring the level of active Rab35 by pull-down assays and for inhibiting the function of Rab35 by overexpression. In this chapter, we describe the assay procedures for analyzing Rab35 with RBD35.作者: 壁畫 時間: 2025-3-26 18:11 作者: Paradox 時間: 2025-3-26 21:44
Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts,ty in cells. This method describes a functional test of GAP activity in cells or extracts that takes into account the presence of other factors or conditions that might change observed in vitro specificity.作者: Cumbersome 時間: 2025-3-27 02:26 作者: 重力 時間: 2025-3-27 06:31 作者: 微粒 時間: 2025-3-27 13:06 作者: JEER 時間: 2025-3-27 17:31
Analysis of the Interactions Between Rab GTPases and Class V Myosins,and organelles such as the endoplasmic reticulum. The Rab GTPases play a critical role in recruiting class V myosins to their cargo. We recently published a study in which we used the yeast two-hybrid system to systematically test myosin Va for its ability to interact with each member of the human R作者: 痛苦一下 時間: 2025-3-27 19:52
Ypt1 and TRAPP Interactions: Optimization of Multicolor Bimolecular Fluorescence Complementation inllular sites of these interactions, we have employed a number of approaches. One approach that we have recently optimized for the use in yeast is multicolor bimolecular fluorescence complementation (BiFC). BiFC, which employs split fluorescent tags, has emerged as a powerful approach for determining作者: PAEAN 時間: 2025-3-28 01:16
Functional Analysis of Rab27A and Its Effector Slp2-a in Renal Epithelial Cells,es as the cells become polarized, and they are specifically localized at the apical membrane in polarized MDCK II cells (i.e., two-dimensional cell culture). Slp2-a is also localized at the apical membrane of tubular MDCK II cysts (i.e., three-dimensional cell culture) and promotes the formation of 作者: 事情 時間: 2025-3-28 05:53
Small GTPases in Acrosomal Exocytosis,s to determine the subcellular localization of active Rabs, something not achievable with other methods. By means of these techniques, we have reported that Rab27 and Rab3 act sequentially and are organized in a RabGEF cascade during the AR. Although we developed them to scrutinize the exocytosis of作者: 意外的成功 時間: 2025-3-28 09:08 作者: NIB 時間: 2025-3-28 10:36 作者: 聽寫 時間: 2025-3-28 17:54 作者: 針葉 時間: 2025-3-28 19:43 作者: 會犯錯誤 時間: 2025-3-29 00:19 作者: 羽毛長成 時間: 2025-3-29 06:09
Matias A. Bustos,Ornella Lucchesi,Maria C. Ruete,Luis S. Mayorga,Claudia N. Tomes作者: excrete 時間: 2025-3-29 10:09
Shreya Mitra,Jennifer Molina,Gordon B. Mills,Jennifer B. Dennison作者: 瘙癢 時間: 2025-3-29 11:46 作者: 大量 時間: 2025-3-29 15:58
Patrick D. Allaire,Peter S. McPherson,Brigitte Ritter作者: 定點 時間: 2025-3-29 21:16 作者: engender 時間: 2025-3-30 01:50 作者: DNR215 時間: 2025-3-30 07:33
Ryan M. Nottingham,Suzanne R. Pfeffert, sie hat ein ansprechendes modernes Layout mit hervorragenden Abbildungen, einen Anhang über die Anwendung von nummerischen Verfahren bei der Berechnung von Magnetfeldern und ?zahlreiche neue durchgerechnete Beispiele mit Praxisbezug. Zus?tzliche Aufgaben mit ausführlichen L?sungen stehen im Internet bereit..978-3-540-89697-5作者: GRIN 時間: 2025-3-30 10:35 作者: audiologist 時間: 2025-3-30 13:20 作者: Inordinate 時間: 2025-3-30 18:33
Shanshan Feng,Bin Wu,Johan Per?nen,Wei Guoeinungen nur von Masse, Raum und Zeit abh?ngig sind, und da? also die Festsetzung von drei Einheiten genügend sein mu? zur Messung aller Gr??en. Ist eine Einheit der Natur entnommen, so hei?t sie ?natürliche Einheit“, ist sie hingegen durch übereinkunft festgesetzt, so hei?t sie ?konventionelle Einh作者: MELD 時間: 2025-3-30 22:21
Zhanna Lipatova,Jane J. Kim,Nava Segevedesmal angeschlossene übergang zum komplexen Potential bringt es dann aber ganz von selbst mit sich, da? auch die andere M?glichkeit, das Feld aus einem zwar vieldeutigen, aber s kalaren Potential abzuleiten, ihre Daseinsberechtigung erf?hrt. Die Kenntnis der Potentialfunktionen in den verschiedene作者: hereditary 時間: 2025-3-31 03:51
Juan Wang,Serena Cervantes,Saralin Davis,Susan Ferro-Novickedesmal angeschlossene übergang zum komplexen Potential bringt es dann aber ganz von selbst mit sich, da? auch die andere M?glichkeit, das Feld aus einem zwar vieldeutigen, aber s kalaren Potential abzuleiten, ihre Daseinsberechtigung erf?hrt. Die Kenntnis der Potentialfunktionen in den verschiedene作者: RAGE 時間: 2025-3-31 05:43
978-1-4939-5594-7Springer Science+Business Media New York 2015作者: 可互換 時間: 2025-3-31 12:48
Rab GTPases978-1-4939-2569-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Ophthalmoscope 時間: 2025-3-31 17:04
Assaying the Interaction of the Rab Guanine Nucleotide Exchange Protein Sec2 with the Upstream Rab,fferent assays that have been used to delineate the mechanisms by which Sec2p, the exchange factor for the Rab Sec4p, is regulated. These assays assess the interaction of Sec2p with the upstream Rab, Ypt32p, a downstream Sec4p effector, Sec15p, and the lipid, phosphatidylinositol-4-phosphate.作者: indigenous 時間: 2025-3-31 20:06 作者: 和音 時間: 2025-3-31 23:13
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820250.jpg作者: FADE 時間: 2025-4-1 04:13 作者: 一個攪動不安 時間: 2025-4-1 08:06 作者: 賞心悅目 時間: 2025-4-1 13:25
Yoan Diekmann,José B. Pereira-Lealef?higt zur qualifizierten Arbeit mit FEM-Programmen.Geeigne.In diesem kompakten Lehrbuch werden die elektrischen und magnetischen Felder so dargestellt, dass sie auch dem mehr praxisorientierten Studenten zug?nglich sind. Hervorzuheben sind die auf der Basis moderner numerischer Verfahren erstellte
