標題: Titlebook: RT-PCR Protocols; Joe O’Connell Book 20021st edition Humana Press 2002 [打印本頁] 作者: 呻吟 時間: 2025-3-21 20:04
書目名稱RT-PCR Protocols影響因子(影響力)
書目名稱RT-PCR Protocols影響因子(影響力)學科排名
書目名稱RT-PCR Protocols網絡公開度
書目名稱RT-PCR Protocols網絡公開度學科排名
書目名稱RT-PCR Protocols被引頻次
書目名稱RT-PCR Protocols被引頻次學科排名
書目名稱RT-PCR Protocols年度引用
書目名稱RT-PCR Protocols年度引用學科排名
書目名稱RT-PCR Protocols讀者反饋
書目名稱RT-PCR Protocols讀者反饋學科排名
作者: 拋媚眼 時間: 2025-3-21 23:28 作者: Cursory 時間: 2025-3-22 03:59
Joe O’Connellseparierbare Netzwerke ausgeschlossen werden. Man nennt ein Netzwerk separierbar, wenn es in zwei Teilnetzwerke zerlegt werden kann, die nicht oder nur über einen Knoten miteinander verbunden sind. Weiterhin sei vorausgesetzt, da? die Elemente in einem Netzwerk so zusammengeschaltet sind, da? keine 作者: 肌肉 時間: 2025-3-22 06:05
Franz Bachmair,Christian G. Huber,Guenter Daxenbichlerpeicherger?te (Pkt. 7.5) weitgehendst entsprochen werden, sofern neben der thermostatisch geregelten Entladung für eine au?entemperaturabh?ngige Aufladung der Ger?te mit jeweiliger Erfassung der in den Ger?ten vom Vortrag allenfalls noch verbliebenen Restw?rme gesorgt ist. Damit werden vor allem W?r作者: HERE 時間: 2025-3-22 09:49
Kaisa Nieminen,Markus Halminen,Matti Waris,Mika M?kel?,Johannes Savolainen,Minna Sj?roos,Jorma Ilonepeicherger?te (Pkt. 7.5) weitgehendst entsprochen werden, sofern neben der thermostatisch geregelten Entladung für eine au?entemperaturabh?ngige Aufladung der Ger?te mit jeweiliger Erfassung der in den Ger?ten vom Vortrag allenfalls noch verbliebenen Restw?rme gesorgt ist. Damit werden vor allem W?r作者: moribund 時間: 2025-3-22 16:21
Jian-Ping Lai,Steven D. Douglas,Wen-Zhe Hompreisen, die dem Strompreis des Falles B entsprechen, kostendeckend w?re. Die Gruppe der direkt elektrisch beheizten Haushalte ist somit als akzeptable Abnehmergruppe anzusehen, genauso wie dies für andere Abnehmer bzw. Abnehmergruppen im gegenst?ndlichen Benutzungsdauerbereich gilt.作者: Motilin 時間: 2025-3-22 19:13 作者: 噴油井 時間: 2025-3-22 23:42
Brian Mullan,Liam J. Fanning,Fergus Shanahan,Daniel G. Sullivaner dem sp?ter behandelten allgemeinsten Falle ungleichartiger Kreise. Kapazit?tswirkungen sollen in den Stromkreisen nicht auftreten. Wir betrachten zun?chst den Verlauf der Ausgleichsstr?me für sich, die in den beiden verketteten Kreisen ohne die Wirkung ?u?erer elektromotorischer Kr?fte flie?en. I作者: 大炮 時間: 2025-3-23 03:37
er dem sp?ter behandelten allgemeinsten Falle ungleichartiger Kreise. Kapazit?tswirkungen sollen in den Stromkreisen nicht auftreten. Wir betrachten zun?chst den Verlauf der Ausgleichsstr?me für sich, die in den beiden verketteten Kreisen ohne die Wirkung ?u?erer elektromotorischer Kr?fte flie?en. I作者: 帶來 時間: 2025-3-23 06:58
Yi Caogebend beeinflu?t. In der Tat erhielten wir im Kapitel 18 unendliche Ausschaltespannungen, wenn wir annahmen, da? der Schalterwiderstand momentan von null auf unendlich gesteigert wurde, und konnten nur dadurch eine Begrenzung der Ausschaltespannung erzielen, da? wir dem Strom einen Nebenweg zur Sch作者: languor 時間: 2025-3-23 11:16 作者: 故意釣到白楊 時間: 2025-3-23 14:45
Detection of mRNA Expression and Alternative Splicing in a Single Cellroy the cell by freezing and thawing, reverse-transcribe RNA, and PCR-amplify. The second step is composed of PCR amplification with another nested PCR-primer set. Using this nested PCR technique, we could detect the expression of FN. We also succeeded in analyzing alternative splicing of FN mRNA in作者: 漂亮 時間: 2025-3-23 20:47 作者: 歌曲 時間: 2025-3-24 00:44
RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus its antigenicity, is believed to hinder humoral immune-mediated clearance of the virus. The ISDR encodes an approx 40 amino-acid sequence in the C-terminal half of the nonstructural 5A (NS5A) protein. Protein kinase inhibition and transcriptional activation activities have been ascribed to NS5A, bo作者: BYRE 時間: 2025-3-24 04:00
Immuno-PCRhigh specificity of immunocytochemistry, we have developed a new method known as . immuno-PCR to detect antigens at low levels in intact cells or tissue sections (.). The concept of . immuno-PCR is illustrated in .. Our study demonstrated that . immuno-PCR is more sensitive for the detection of Hepa作者: 圓木可阻礙 時間: 2025-3-24 06:51
Book 20021st editionations of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data f作者: 分解 時間: 2025-3-24 14:19 作者: 灌輸 時間: 2025-3-24 16:55 作者: Eructation 時間: 2025-3-24 21:13
Triona Goode,Wen-Zhe Ho,Terry O’Connor,Sandra Busteed,Steven D. Douglas,Fergus Shanahan,Joe O’Connel作者: forestry 時間: 2025-3-24 23:46
Joe O’Connell,Aileen Houston,Raymond Kelly,Darren O’Brien,Aideen Ryan,Michael W. Bennett,Kenneth Nal作者: Adenocarcinoma 時間: 2025-3-25 06:14
Book 20021st editiont. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the nee作者: Albinism 時間: 2025-3-25 07:46
Rapid Development of a Quantitative-Competitive (qc) RT-PCR Assay Using a Composite Primer Approachnd a shift in the equilibrium of template denaturation, which favors association rather than denaturation of the template DNA strands as product concentration becomes high. This results in a “plateau effect,” such that there is no linearity in the relationship between product yield and initial template.作者: Scintigraphy 時間: 2025-3-25 15:06
Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Productsuantitation (.). Recently, real-time PCR, in which the generated PCR-products are quantified fluorimetrically after each cycle, has become widely used (.). However, determination of fragment size for positive fragment identification is not—at least not directly—possible with this method.作者: 著名 時間: 2025-3-25 18:33
RT-PCR in Biomedicineechnique permits specific mRNA to be detected and quantified has been a major asset in the molecular investigation of disease pathogenesis. Disease-related imbalances in the expression of specific mRNAs can be sensitively and quantitatively determined by RT-PCR. RT-PCR also offers many opportunities作者: 欲望小妹 時間: 2025-3-25 23:33
The Basics of RT-PCRe fundamental considerations for such basics as primer design, good laboratory set up and practice, and techniques for performing RT-PCR have been fully examined elsewhere (.,.). Thus, I will include only observations from my own experience in this chapter. Although the purpose of this chapter is to作者: collagenase 時間: 2025-3-26 01:14 作者: Foreknowledge 時間: 2025-3-26 06:26 作者: fructose 時間: 2025-3-26 09:02
Nested RT-PCRed by performing “nested” RT-PCR. This involves taking an aliquot of the product from the primary RT-PCR, and using it as a template for a secondary round of PCR amplification. To avoid further amplification of primer-dimer artifacts or nonspecific products generated in the primary PCR, a different 作者: 新手 時間: 2025-3-26 13:55
Quantitative RT-PCRost sensitive technique available for mRNA detection and quantification. It can accurately quantify genes present at only a few hundred copies per sample, and has become the method of choice for the examination of gene expression. The technique consists of two parts: synthesis of cDNA from RNA by re作者: LAPSE 時間: 2025-3-26 17:56 作者: carotenoids 時間: 2025-3-26 23:54
Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Productstive method requiring microgram amounts of RNA. It is time consuming and semi-quantitative at best. Because of the limitations of Northern blotting, various strategies have been developed for quantitation of cDNA by polymerase chain reaction (PCR)-based methods (.–.). Competitive PCR, in which a syn作者: 隱士 時間: 2025-3-27 01:34 作者: BLOT 時間: 2025-3-27 06:29 作者: 吼叫 時間: 2025-3-27 11:28 作者: 一起平行 時間: 2025-3-27 17:13
Semi-Quantitative Detection of Hepatitis C Virus RNA by “Real-Time” RT-PCRol to tailor and monitor antiviral therapies for this disease (.). Although a low viral load is associated with a higher efficacy of Interferonm (IFN)-α/ribavirin combination therapies, patients with a high viral load respond more poorly to this treatment and require a longer duration of therapy. In作者: Ablation 時間: 2025-3-27 21:40
RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virusen an intensive focus on understanding the underlying biology of its disease process. Of significant interest has been the study of heterogenous populations of closely related, but genetically non-identical HCV virions, commonly termed quasispecies (.,.), and their relationship to the pathogenesis o作者: 大門在匯總 時間: 2025-3-27 22:49 作者: 惡名聲 時間: 2025-3-28 02:25 作者: STERN 時間: 2025-3-28 08:47
Quantitative RT-PCRverse transcription, and amplification of a specific cDNA by polymerase chain reaction (PCR). The method requires very little RNA and differs from Northern blotting because it is somewhat tolerant of degraded RNA, as long as the RNA is intact within the region of interest.作者: 變白 時間: 2025-3-28 11:49
Detection and Quantification of the Hepatitis C Viral Genomeen instrumental in assessing the natural history of HCV, in which viral RNA levels have a large dynamic range. Assessment of the HCV viral load is now a routine part of the algorithm for the diagnosis of chronic hepatitis C (.–.). Patient’s response to anti-viral therapy can be assessed, in part, by quantification of serum viral loads (.,.).作者: 新陳代謝 時間: 2025-3-28 17:35
1064-3745 undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to ra作者: 一夫一妻制 時間: 2025-3-28 22:24
Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in was provided by Wang et al. (.). As an internal standard, an RNA template with the same primer sites as the target mRNA was reverse-transcribed. The amplified PCR products differed in size and were separated by agarose gel electrophoresis. The presence of .P-labeled 5′ primer allowed quantification by scintillation counting.作者: DUCE 時間: 2025-3-29 00:39
Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytesding murine macrophages (.,.), human endothelial cells (.,.), eosinophils (.), and Leydig cells derived from human and mouse testis (.). We have demonstrated that human monocytes and lymphocytes express the SP gene mRNA transcripts and produce SP protein (.–.).作者: 充氣女 時間: 2025-3-29 03:41
Semi-Quantitative Detection of Hepatitis C Virus RNA by “Real-Time” RT-PCR-α/ribavirin combination therapies, patients with a high viral load respond more poorly to this treatment and require a longer duration of therapy. In addition, the HCV-RNA status in serum during treatment is used as a marker for the short-term success of therapy.作者: 因無茶而冷淡 時間: 2025-3-29 09:04
Nested RT-PCRer amplicon (.). Because their sequences are different from the primary set of primers, they will not amplify artifacts or nonspecific products generated in the primary PCR, enabling product specificity to be maintained over the high number of amplification cycles combined in the primary and secondary PCRs.作者: Admire 時間: 2025-3-29 11:44 作者: 完成才能戰(zhàn)勝 時間: 2025-3-29 17:26
Joe O’ConnellIncludes supplementary material: 作者: Water-Brash 時間: 2025-3-29 22:17 作者: 煉油廠 時間: 2025-3-30 01:50 作者: Canyon 時間: 2025-3-30 06:59
om-Spannungsbeziehungen für die Netzwerkelemente bestimmt. Im folgenden sollen systematische Verfahren entwickelt werden, die dazu geeignet sind, auch kompliziertere Netzwerke in verh?ltnism??ig einfacher Weise zu analysieren und allgemeine Aussagen zu machen. Unter einem Netzwerk wird im Sinne von 作者: 光亮 時間: 2025-3-30 08:59
