標題: Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2016 Springer Science+Business Media New York 2016 RNA-bindi [打印本頁] 作者: Annihilate 時間: 2025-3-21 17:45
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書目名稱RNA-Protein Complexes and Interactions網絡公開度學科排名
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書目名稱RNA-Protein Complexes and Interactions被引頻次學科排名
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書目名稱RNA-Protein Complexes and Interactions讀者反饋
書目名稱RNA-Protein Complexes and Interactions讀者反饋學科排名
作者: ARBOR 時間: 2025-3-21 20:29 作者: 膠狀 時間: 2025-3-22 04:08
Ren-Jang LinIncludes cutting-edge methods and protocols for studying RNA-protein interactions.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the 作者: aneurysm 時間: 2025-3-22 05:53 作者: labyrinth 時間: 2025-3-22 11:53 作者: Polydipsia 時間: 2025-3-22 13:16
Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases,s can be directly assigned to the steps associated with the chemistry of methyl transfer, including the substrate binding affinity to the methyltransferase, the pre-chemistry re-arrangement of the active site, and the chemical step of methyl transfer. An additional advantage is that kinetic constant作者: FIS 時間: 2025-3-22 21:04
,Genome-Wide Profiling of RNA–Protein Interactions Using CLIP-Seq,ence genome and the identification of the crosslinking sites. Meanwhile, one or more amino acids of a crosslinked RBP can remain attached to its bound RNA due to incomplete digestion of the protein. As a result, reverse transcriptase may not read through the crosslink sites, and produce cDNA ending 作者: 流浪者 時間: 2025-3-22 21:41
Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 anr U1” snRNAs with restored base-pairing to mutant 5′ss in different registers. Cells are transfected with combinations of minigenes and suppressor U1s, and the splicing patterns are analyzed by reverse transcription and semiquantitative PCR, followed by gel electrophoresis. The identity of suppresso作者: GNAT 時間: 2025-3-23 05:26 作者: 撤退 時間: 2025-3-23 07:57 作者: foreign 時間: 2025-3-23 11:39 作者: 血統 時間: 2025-3-23 15:41 作者: Exonerate 時間: 2025-3-23 19:22
Shuai Hou,Lei Shi,Haixin LeiShelly Goldstein, Daniel Greenberger, Lucien Hardy, Anthony Leggett, Tim Maudlin, David Mermin, Lee Smolin, Antony Valentini, David Wallace, Anton Zeilinger, and Wojciech Zurek. .978-3-642-26981-3978-3-642-20880-5Series ISSN 1612-3018 Series E-ISSN 2197-6619 作者: Infantry 時間: 2025-3-24 01:15 作者: dandruff 時間: 2025-3-24 06:26
Keith T. Gagnon Ph.D.Shelly Goldstein, Daniel Greenberger, Lucien Hardy, Anthony Leggett, Tim Maudlin, David Mermin, Lee Smolin, Antony Valentini, David Wallace, Anton Zeilinger, and Wojciech Zurek. .978-3-642-26981-3978-3-642-20880-5Series ISSN 1612-3018 Series E-ISSN 2197-6619 作者: 馬賽克 時間: 2025-3-24 07:58
Hua-Lin Zhou,Hua LouShelly Goldstein, Daniel Greenberger, Lucien Hardy, Anthony Leggett, Tim Maudlin, David Mermin, Lee Smolin, Antony Valentini, David Wallace, Anton Zeilinger, and Wojciech Zurek. .978-3-642-26981-3978-3-642-20880-5Series ISSN 1612-3018 Series E-ISSN 2197-6619 作者: inflame 時間: 2025-3-24 11:23 作者: glamor 時間: 2025-3-24 17:58 作者: 人類學家 時間: 2025-3-24 21:28
Cheryl Stork,Sika ZhengShelly Goldstein, Daniel Greenberger, Lucien Hardy, Anthony Leggett, Tim Maudlin, David Mermin, Lee Smolin, Antony Valentini, David Wallace, Anton Zeilinger, and Wojciech Zurek. .978-3-642-26981-3978-3-642-20880-5Series ISSN 1612-3018 Series E-ISSN 2197-6619 作者: TOXIN 時間: 2025-3-25 02:08 作者: profligate 時間: 2025-3-25 03:19
Lindsey Skrdlant,Ren-Jang Lingaluppi, Caslav Brukner, Jeffrey Bub, Arthur Fine, Christopher Fuchs, GianCarlo Ghirardi, Shelly Goldstein, Daniel Greenberger, Lucien Hardy, Anthony Leggett, Tim Maudlin, David Mermin, Lee Smolin, Antony Valentini, David Wallace, Anton Zeilinger, and Wojciech Zurek. .作者: 主動脈 時間: 2025-3-25 10:15
Naoyuki Kataokagaluppi, Caslav Brukner, Jeffrey Bub, Arthur Fine, Christopher Fuchs, GianCarlo Ghirardi, Shelly Goldstein, Daniel Greenberger, Lucien Hardy, Anthony Leggett, Tim Maudlin, David Mermin, Lee Smolin, Antony Valentini, David Wallace, Anton Zeilinger, and Wojciech Zurek. .作者: PHONE 時間: 2025-3-25 14:17 作者: BROOK 時間: 2025-3-25 16:06 作者: Noctambulant 時間: 2025-3-25 21:03
Sarah F. Mitchell,Roy Parkers bzw. die Verst?rkung einer bereits bestehenden Hypertonie bei einem Teil der Patienten. Sowohl die Ergebnisse der Tiermodellstudien als auch die klinischen Ergebnisse bei Patienten im Eigenblutspendeprogramm zeigen, da? eine Therapie mit rhEPO in dieser Indikation nicht mit dem aus der Behandlung 作者: FRAX-tool 時間: 2025-3-26 01:06 作者: 不利 時間: 2025-3-26 06:32
Fu-Lung Yeh,Luh Tung,Tien-Hsien Changs bzw. die Verst?rkung einer bereits bestehenden Hypertonie bei einem Teil der Patienten. Sowohl die Ergebnisse der Tiermodellstudien als auch die klinischen Ergebnisse bei Patienten im Eigenblutspendeprogramm zeigen, da? eine Therapie mit rhEPO in dieser Indikation nicht mit dem aus der Behandlung 作者: 脖子 時間: 2025-3-26 09:01 作者: Compass 時間: 2025-3-26 12:56 作者: 抗原 時間: 2025-3-26 18:32
Book 2016 introductory chapter,the book continues with ways to purify RNA-protein complexes assembled in cellsor in isolated cellular extracts, methods for measuring various biochemicalactivities of RNA-interacting proteins or ribonucleoproteins, biochemicalmethods for measuring direct RNA-protein contact, a作者: BRINK 時間: 2025-3-26 21:05 作者: 粗魯性質 時間: 2025-3-27 04:23 作者: 刺耳的聲音 時間: 2025-3-27 09:01 作者: 灰姑娘 時間: 2025-3-27 11:11
Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichmentidentifying yeast mRNA-binding proteins in a systematic manner using UV crosslinking, purification of polyA(+) mRNAs under denaturing conditions, and mass spectrometry to identify covalently bound proteins.作者: Bronchial-Tubes 時間: 2025-3-27 16:45
,Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reactihere to prepare splicing-active extracts from whole cells is particularly useful when studying pre-mRNA splicing in various cell types, and the expression of a tagged spliceosomal protein allows one to purify and analyze the RNA–protein complexes by simple immunoprecipitation.作者: Cloudburst 時間: 2025-3-27 21:08 作者: Endometrium 時間: 2025-3-27 23:41
Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Spling factors with diverse activities in regulating different types of alternative splicing. The artificial splicing factors can be used to change splicing of either minigenes or endogenous genes in cultured human cells, providing a new strategy to study the regulation of alternative splicing and function of alternatively spliced products.作者: endure 時間: 2025-3-28 03:51 作者: echnic 時間: 2025-3-28 09:13
Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles,ny target site. Here, we describe a method for the reconstitution of functional box H/ACA RNPs using designer box H/ACA guide RNAs, which in principle can be adopted to reconstitute other RNA–protein complexes as well.作者: allergen 時間: 2025-3-28 10:25 作者: inchoate 時間: 2025-3-28 15:24 作者: 創(chuàng)作 時間: 2025-3-28 20:04
,Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro,ion of RNA in nuclear extract and fractionated using gel filtration, and RNP fractions are pooled for biotin–streptavidin affinity purification. The amount of RNA–protein complexes purified following this protocol is sufficient for mass spectrometry.作者: neutralize 時間: 2025-3-29 02:46 作者: 謙虛的人 時間: 2025-3-29 06:44 作者: 兒童 時間: 2025-3-29 09:13 作者: 技術 時間: 2025-3-29 12:28
mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA-Binding Protei motif conservation, RNA secondary structure, and the tendency of RBP binding sites to cluster together. A probabilistic model is learned from bona fide binding sites determined by CLIP and applied genome wide to generate high specificity binding site predictions.作者: 破譯密碼 時間: 2025-3-29 16:50
1064-3745 sults.Contains key notes and implementation advice from the .This detailed volume explores the continuing techniques ofstudying RNA-protein complexes and interactions as research in these areasexpand.? After an introductory chapter,the book continues with ways to purify RNA-protein complexes assembl作者: Pigeon 時間: 2025-3-29 21:23
,Characterization of RNA–Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF the SR protein family were once thought to have redundant functions, in-depth biochemical analysis of their RNA-binding abilities has revealed distinct binding profiles for each SR protein, that often lead to either synergistic or antagonistic functions. SR protein family members SRSF1 and SRSF2 ar作者: 拍下盜公款 時間: 2025-3-30 02:17
Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification),RBPs and ncRNAs jointly influence all aspects of posttranscriptional metabolism, including pre-mRNA splicing and maturation, mRNA transport, editing, stability, and translation. Given the impact of mRNA-interacting molecules on gene expression, there is great interest in identifying mRNA-binding fac作者: 難取悅 時間: 2025-3-30 06:17 作者: COUCH 時間: 2025-3-30 09:10 作者: Overdose 時間: 2025-3-30 12:36 作者: jovial 時間: 2025-3-30 17:53
Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts,to regulate gene expression through RNAi they must be loaded, or “programmed,” with a single strand of small RNA. Natural small RNAs are typically double-stranded duplexes that require additional factors for efficient and specific loading into Ago proteins. Here, a protocol is described for investig作者: Ptsd429 時間: 2025-3-30 22:42 作者: Phenothiazines 時間: 2025-3-31 01:04 作者: fastness 時間: 2025-3-31 05:41
Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles,. In eukaryotes and archaea, RNA pseudouridylation is catalyzed largely by box H/ACA ribonucleoproteins (RNPs), a distinct group of RNA–protein complexes each consisting of a unique RNA and four common proteins. The RNA component of the complex serves as a guide that base-pairs with its substrate RN作者: CAMEO 時間: 2025-3-31 10:02
,Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixtu to prepare .P-labeled RNA probes and to use them to assay for RNA–protein interactions after partially purified protein preparations are resolved on denaturing SDS-polyacrylamide gels. The method can unambiguously determine whether the protein of interest can directly and independently bind RNA eve作者: 偽造者 時間: 2025-3-31 15:13 作者: 失望未來 時間: 2025-3-31 21:07 作者: 內部 時間: 2025-4-1 01:03
Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichmentation of the complete set of mRNA-binding proteins is a key step in understanding the regulation of gene expression. Herein, we describe a method for identifying yeast mRNA-binding proteins in a systematic manner using UV crosslinking, purification of polyA(+) mRNAs under denaturing conditions, and 作者: hemorrhage 時間: 2025-4-1 05:05
,Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks,many cases, these interactions are transient and highly dynamic. Therefore, capturing stable as well as transient interactions in living cells for the identification of protein-binding partners and the mapping of RNA-binding sequences is key to a successful establishment of the molecular interaction作者: 耐寒 時間: 2025-4-1 09:24
,Detection of Protein–Protein Interaction Within an RNA–Protein Complex Via Unnatural-Amino-Acid-Medction to remodel RNA–protein complexes. Precisely how the latter is achieved remains a mystery. We investigated this critical issue by using yeast Prp28p, an evolutionarily conserved DExD/H-box splicing factor, as a model system. To probe how Prp28p interacts with spliceosome, we strategically place作者: Bronchial-Tubes 時間: 2025-4-1 10:59
Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX,d to a cell-surface antigen or a particular target cell population. In particular, it offers a facile selection strategy for some special cell-surface proteins that are original glycosylated or heavily post-translationally modified, and are unavailable in their native/active conformation after in vi作者: 和諧 時間: 2025-4-1 16:37 作者: 知道 時間: 2025-4-1 21:14
Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Spliing have been found to be closely associated with various human diseases; thus new approaches to modulate disease-associated splicing events will provide great therapeutic potentials. Here we report protocols for constructing novel artificial splicing factors that can be designed to specifically mod
