標(biāo)題: Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2015 Springer Science+Business Media New York 2015 DEAD-box Helicase.EM [打印本頁] 作者: deep-sleep 時間: 2025-3-21 16:33
書目名稱RNA Remodeling Proteins影響因子(影響力)
作者: 字形刻痕 時間: 2025-3-21 20:18
Atsushi Furuta,Kazi Abdus Salam,Hidenori Tani,Satoshi Tsuneda,Yuji Sekiguchi,Nobuyoshi Akimitsu,Naoh作者: 生來 時間: 2025-3-22 01:47
Kimberly A. Reynolds,Veronica M. Raney,Kevin D. Raney作者: Inscrutable 時間: 2025-3-22 06:38 作者: 巡回 時間: 2025-3-22 12:46 作者: 消毒 時間: 2025-3-22 16:53 作者: 窩轉(zhuǎn)脊椎動物 時間: 2025-3-22 17:23 作者: minimal 時間: 2025-3-23 00:51
Dynamics of the Spb4 Interactome Monitored by Affinity Purification,s has been established as one of the most useful techniques to explore the yeast ribosome biogenesis pathway. In this chapter, we describe the use of different affinity purification methods to study the physical environment of RNA helicases involved in ribosome biogenesis, using as an example the pu作者: 膽大 時間: 2025-3-23 03:37 作者: mighty 時間: 2025-3-23 05:51
Cellular Localization of RNA Degradation and Processing Components in ,,ion of labeled proteins. In addition, when antibodies against native proteins are not available or lack specificity, epitope tags such as hemagglutinin (HA) or Flag can be used to label chromosomally expressed proteins. The short Flag- and HA-tag, eight or nine amino acids, are unlikely to interfere作者: Exhilarate 時間: 2025-3-23 10:28 作者: Verify 時間: 2025-3-23 14:48
Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-Box f interest, which we modify by classical molecular biological techniques (mutations and deletions). We then use various biochemical techniques to characterize the purified proteins and their variants for their ATPase, RNA binding, and RNA unwinding activities to determine the functional roles of the作者: Isometric 時間: 2025-3-23 19:10
A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitorh a fluorophore-labeled strand hybridized to a quencher-labeled strand and monitors the increase in fluorescence intensity resulting from helicase-catalyzed unwinding of the dsRNA substrate. We further describe radioactive assays to directly visualize RNA strands unwound by helicase and to evaluate 作者: GEAR 時間: 2025-3-24 00:58 作者: 做方舟 時間: 2025-3-24 03:41
https://doi.org/10.1007/978-1-4939-2214-7DEAD-box Helicase; EMOTE; Fluorescence Resonance Energy Transfer (FRET); RHAU; RNA Helicase; RNA targets; 作者: 褲子 時間: 2025-3-24 08:23 作者: 不連貫 時間: 2025-3-24 10:40
Determination of RNA Chaperone Activity Using an , Mutant,splays a cold-sensitive growth phenotype. Plant cold shock domain (CSD) proteins have been shown to complement the cold-sensitive phenotype of the . mutant and to share a function with . CSPs as RNA chaperones. This methodology, which is detailed here, can be utilized to reveal or probe the RNA chaperone activity of heterologous proteins.作者: atrophy 時間: 2025-3-24 17:28
Probing RNA Translocases with DNA,manganate (KMnO.) footprinting is a method used to chemically probe the conformation of DNA as well as the binding of proteins. Combining footprinting methods with rapid mixing methods that utilize a chemical quench-flow instrument can enable tracking of the translocase with nucleotide resolution.作者: BRACE 時間: 2025-3-24 21:11 作者: 哀悼 時間: 2025-3-25 01:30
RNA Remodeling Proteins978-1-4939-2214-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 粗糙 時間: 2025-3-25 03:43
Key Points to Consider When Studying RNA Remodeling by Proteins,dence arises from intrinsic properties of RNA structure. Specifically, RNAs possess stable local structure, largely in the form of helices, and they have abundant opportunities to form alternative helices and tertiary contacts and therefore to populate alternative structures. Proteins with RNA chape作者: 放大 時間: 2025-3-25 10:07 作者: 不妥協(xié) 時間: 2025-3-25 12:31
In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins, understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spe作者: 要求比…更好 時間: 2025-3-25 17:25
Dynamics of the Spb4 Interactome Monitored by Affinity Purification,ukaryotic model organism .. In yeast, genetic and biochemical analyses indicate that these RNA helicases are energy-consuming modulators of local structures inside pre-ribosomal particles that actively promote the establishment or dissociation of snoRNA:pre-rRNA base pairings, the activity of certai作者: Canvas 時間: 2025-3-25 21:34
,Using EMOTE to Map the Exact 5′-Ends of Processed RNA on a Transcriptome-Wide Scale,oswitches, but it is also the case for many mRNAs, where secondary structures in the 5′ or 3′ UTR can determine the efficiency of translation or the half-life of the RNA. There are paths to modify such secondary structures, (1) by the action of a helicase that allows an alternative RNA structure to 作者: CAND 時間: 2025-3-26 03:07 作者: 陳腐思想 時間: 2025-3-26 04:23 作者: 拔出 時間: 2025-3-26 10:30
Determination of RNA Chaperone Activity Using an , Mutant,splays a cold-sensitive growth phenotype. Plant cold shock domain (CSD) proteins have been shown to complement the cold-sensitive phenotype of the . mutant and to share a function with . CSPs as RNA chaperones. This methodology, which is detailed here, can be utilized to reveal or probe the RNA chap作者: Blazon 時間: 2025-3-26 12:45 作者: 職業(yè) 時間: 2025-3-26 18:37 作者: Circumscribe 時間: 2025-3-27 00:52
Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-Box binding ATP and RNA, and that are needed for the enzymatic activities. The conserved sequence motifs and structural homology indicate that these proteins share common origins and underlining functionality. Indeed, the purified proteins generally act as ATP-dependent RNA-binding proteins and RNA-dep作者: 破譯密碼 時間: 2025-3-27 02:25
Measuring Helicase Inhibition of the DEAD-Box Protein Dbp2 by Yra1,ines DEAD-box protein specificity is enzymatic regulation by other protein cofactors. In this chapter, we describe a protocol for purifying the . DEAD-box RNA helicase Dbp2 and RNA-binding protein Yra1 and subsequent analysis of helicase regulation. The experiments described here can be adapted to o作者: 訓(xùn)誡 時間: 2025-3-27 07:05 作者: ORE 時間: 2025-3-27 11:28 作者: EVADE 時間: 2025-3-27 16:38 作者: 壯麗的去 時間: 2025-3-27 19:14 作者: Blanch 時間: 2025-3-28 01:11 作者: 提升 時間: 2025-3-28 05:58
Monitoring RNA Unwinding by the Transcription Termination Factor Rho from ,,iptional RNA–DNA duplexes (R-loops) in bacteria. The molecular mechanisms underlying these biological functions have been essentially studied with Rho enzymes from . or close Gram-negative relatives. However, phylo-divergent Rho factors may have distinct properties. Here, we describe methods for the作者: 坦白 時間: 2025-3-28 09:30
Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1,pled processes. Here we describe an in vitro transcription-termination assay that we have used for the analysis of the mechanism of termination by the yeast helicase Sen1. In this system, we use highly purified proteins to assemble ternary elongation complexes (RNA polymerase, DNA template, and nasc作者: Esalate 時間: 2025-3-28 11:56 作者: 出來 時間: 2025-3-28 16:54
Recombineering Applications for the Mutational Analysis of Bacterial RNA-Binding Proteins and Theirb neighboring sequences. Methods for performing such “markerless” site-directed chromosomal mutagenesis in bacteria have been developed in recent years. One such technique, used routinely in our laboratory, is described here.作者: 卵石 時間: 2025-3-28 18:47
A FRET-Based, Continuous Assay for the Helicase Activity of DEAD-Box Proteins,ion of duplexes and isolated strands. Here, we describe a continuous, FRET-based assay that allows the measurement of the helicase activities of DEAD-box proteins with a precision high enough to detect changes in activation enthalpies associated with cold adaptation.作者: 織物 時間: 2025-3-29 02:08
Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-Molecule Level,nstruction of a simple magnetic trap and, in particular, on the detailed description of image analysis methods to measure the extension changes in nucleic-acid molecules induced by protein activity. Finally, we carefully describe the steps involved in performing a full magnetic tweezers experiment.作者: 耐寒 時間: 2025-3-29 06:00
Monitoring RNA Unwinding by the Transcription Termination Factor Rho from ,, preparation and in vitro characterization (ATPase and helicase activities) of the Rho factor from ., a specimen with uncharacteristic molecular and enzymatic features. These methods set the stage for future studies aimed at better defining the diversity of enzymatic properties of Rho across the bacterial kingdom.作者: ARBOR 時間: 2025-3-29 10:10 作者: 香料 時間: 2025-3-29 12:18 作者: Sinus-Rhythm 時間: 2025-3-29 16:03
Key Points to Consider When Studying RNA Remodeling by Proteins,roteins that function as chaperones and remodelers. We then use these properties as a foundation to explore key points for the design and interpretation of experiments that probe RNA rearrangements and their acceleration by proteins.作者: 帶來墨水 時間: 2025-3-29 22:59
Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU,bination of total RNA and quadruplex-specific dyes to observe the expected quadruplex to duplex conversion. This straightforward method can be extended to study structural changes in other inter- or intramolecular quadruplex containing DNA/RNA molecules with the RHAU helicase or other RNA/DNA remodeling enzymes.作者: abstemious 時間: 2025-3-30 02:58
Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers,resolution optical tweezers study of NS3 helicase, starting from the purification of the recombinant helicase protein from . to setting up a high-resolution single-molecule experiment using optical tweezers.作者: omnibus 時間: 2025-3-30 06:55 作者: ILEUM 時間: 2025-3-30 11:24
Book 2015 community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiologic作者: MELD 時間: 2025-3-30 12:45
Autonomic Neuropathies,athetic, parasympathetic, and enteric nervous systems. Disorders of the ANS can lead to cardiovascular, sudomotor, gastrointestinal, or urogenital dysfunction. When addressing potential disorders of the ANS, a detailed history provides essential information on which autonomic domains are involved, a作者: 銀版照相 時間: 2025-3-30 17:41 作者: Mercurial 時間: 2025-3-30 21:06
A Personal Encounter: Some Reflections on Communication for Development and Social Change,on from the 1970s onwards, and a summary of a number of more systematic meta-analyses, based on academic publications in the same period. It highlights the major shifts which took place at theoretical, professional and institutional levels.作者: staging 時間: 2025-3-31 04:53 作者: 寒冷 時間: 2025-3-31 07:48 作者: ILEUM 時間: 2025-3-31 10:06
Kolumban Hutter,Yongqi Wang,Irina P. Chubarenkoht, developing the strategies to combat racism in and out of schools is vital. These new strategies involve interrogating societal norms that reproduce the issues of racism and education that foster societal reproduction of racial structures.作者: subacute 時間: 2025-3-31 15:36 作者: 贊美者 時間: 2025-3-31 17:47 作者: 智力高 時間: 2025-4-1 00:14 作者: stress-response 時間: 2025-4-1 04:29
Bodo Selmke,Stefan Brummer,Johann Heyszl,Georg Sigl It then goes on to detail the internal representations and local functions of larger wholes in smaller communities, shedding a new light on issues of inter- disciplinary interest: self- governance, participation, local knowledge, social memory, scale, planning and development..Of interest to politi作者: FIR 時間: 2025-4-1 09:09 作者: insolence 時間: 2025-4-1 10:20
2194-5357 ciences. In the last few years, we have seen the surge of a new generation of interdisciplinary scientists that have a strong background in the biological and computational sciences. In this context, the intera978-3-319-00577-5978-3-319-00578-2Series ISSN 2194-5357 Series E-ISSN 2194-5365 作者: delegate 時間: 2025-4-1 18:16
Personalmanagement im Umbruchuktivit?tsfortschritte ergeben sich durch die Verlagerung einfacher Produktions- und Montagearbeiten in Schwellenl?nder, die Substitution von Arbeit durch Technik sowie durch eine verst?rkte Flexibilit?t und Proze?orientierung im Wertsch?pfungsproze?.作者: Brittle 時間: 2025-4-1 21:55 作者: 注視 時間: 2025-4-1 23:57
