作者: 江湖騙子 時(shí)間: 2025-3-21 21:56 作者: Champion 時(shí)間: 2025-3-22 02:10 作者: 散步 時(shí)間: 2025-3-22 05:56 作者: enormous 時(shí)間: 2025-3-22 12:03 作者: 破譯密碼 時(shí)間: 2025-3-22 14:07 作者: BILL 時(shí)間: 2025-3-22 20:54 作者: Exhilarate 時(shí)間: 2025-3-22 23:11
Monitoring Global Structural Changes and Specific Metal-Ion-Binding Sites in RNA by In-line Probing structure, and function. Aside from revealing directly metal ion-binding sites these techniques also provide structural information for longer RNA sequences that are out of range to be analyzed with other techniques such as NMR. The cleavage pattern derived from in-line probing experiments reflects作者: Ptsd429 時(shí)間: 2025-3-23 02:44 作者: SYN 時(shí)間: 2025-3-23 07:06
A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding,ne expression. In order to correctly perform its function in the cell, an RNA molecule must fold into a complex tertiary structure. Folding of many large RNAs is slow either due to formation of stable misfolded intermediates or due to high contact order or instability of obligate folding intermediat作者: evince 時(shí)間: 2025-3-23 10:50 作者: Ordnance 時(shí)間: 2025-3-23 14:13
The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis,ndard protein enzymes, for which reactions can usually be started by mixing the enzyme with its substrate, ribozymes are typically self-cleaving. The reaction has to be initiated by folding the RNA into its active conformation. Thus, ribozyme kinetics are influenced by both folding and catalytic com作者: 殘暴 時(shí)間: 2025-3-23 19:17
RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding,RNA chaperone proteins on the folding rate. The crux of this approach is that only the natively folded RNA is able to perform the catalytic reaction. This method can provide a quantitative measure of the fraction of native RNA over time, and it can readily distinguish the native state from all misfo作者: 別炫耀 時(shí)間: 2025-3-24 02:03 作者: 開(kāi)始沒(méi)有 時(shí)間: 2025-3-24 06:08
RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis,formation about such native structures in solution as a function of experimental conditions. The application of native gel electrophoresis in a comparative manner allows to obtain precise information on relative angles subtended between given pair of stems in an RNA molecule. By adapting this approa作者: fulmination 時(shí)間: 2025-3-24 09:41
Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation,r interactions in solution. When operated in a sedimentation velocity (SV) recording mode, it reports precisely on the hydrodynamic properties of a molecule, including its sedimentation and diffusion coefficients, which can be used to calculate its hydrated radius, as well as, to estimate its global作者: 方便 時(shí)間: 2025-3-24 11:17
RNA Folding Dynamics Using Laser-Assisted Single-Molecule Refolding,ys is critical to understanding how RNA molecules acquire their biological function. We have previously developed a novel approach to help characterize such misfolded intermediates. Laser-assisted single-molecule refolding (LASR) is a powerful technique that combines temperature-jump (T-jump) kineti作者: 生命層 時(shí)間: 2025-3-24 16:51 作者: 咯咯笑 時(shí)間: 2025-3-24 19:30
Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Smallopy (FCS) and small angle X-ray scattering (SAXS) measurements can be used to determine intermolecular interactions between RNA and its binding partners. To define oligomerization states of free RNA and its complexes with bound target molecules, hydrodynamic radii, radii of gyration as well as the m作者: 雇傭兵 時(shí)間: 2025-3-25 02:58
1064-3745 ential for reproducible results.Contains key notes and vital.Focusing on in vitro and intracellular RNA structure formation, .RNA Folding: Methods and Protocols. provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the s作者: 支形吊燈 時(shí)間: 2025-3-25 03:43 作者: parsimony 時(shí)間: 2025-3-25 09:06 作者: ALTER 時(shí)間: 2025-3-25 13:10 作者: 激怒 時(shí)間: 2025-3-25 19:07 作者: acrobat 時(shí)間: 2025-3-25 21:37
A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding,ve state under physiological conditions is facilitated by the protein cofactor Mss116. This chapter describes the use of Nucleotide Analog Interference Mapping (NAIM) to identify specific substructures within the intron molecule that are directly affected by the protein.作者: 鋼盔 時(shí)間: 2025-3-26 00:48 作者: BOOM 時(shí)間: 2025-3-26 05:22 作者: 截?cái)?nbsp; 時(shí)間: 2025-3-26 12:18 作者: CHIDE 時(shí)間: 2025-3-26 15:18
Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes,ity of all four nucleobases can be determined. With this fast and inexpensive method local changes in RNA secondary and tertiary structure, as well as the formation of contacts between RNA and its ligands can be detected independent of the RNA’s length.作者: 拒絕 時(shí)間: 2025-3-26 20:01 作者: 翻動(dòng) 時(shí)間: 2025-3-26 23:39 作者: Congruous 時(shí)間: 2025-3-27 04:08 作者: PANEL 時(shí)間: 2025-3-27 07:56 作者: Femish 時(shí)間: 2025-3-27 12:10
RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis,ch, it is possible to obtain very specific structural information such as the amplitude of dihedral angles and helical rotation. As an example, we will describe how native gel electrophoresis can be used to study the folding of the .-adenosylmethionine (SAM) sensing riboswitch.作者: 悅耳 時(shí)間: 2025-3-27 14:08 作者: adumbrate 時(shí)間: 2025-3-27 21:21 作者: 漂泊 時(shí)間: 2025-3-27 22:43 作者: 樹(shù)木中 時(shí)間: 2025-3-28 03:39
978-1-4939-6058-3Springer Science+Business Media, LLC 2014作者: 磨碎 時(shí)間: 2025-3-28 07:52
RNA Folding978-1-62703-667-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Postulate 時(shí)間: 2025-3-28 12:33 作者: LEERY 時(shí)間: 2025-3-28 14:54
Ivo L. Hofacker,Ronny Lorenze before-mentioned task. Firstly, a data warehouse will be built that will collect and load the data from disparate sources in a data schemes within the data warehouse that will suit data analytics. We will analyze the E-government architecture, public administration processes and the available tech作者: Accord 時(shí)間: 2025-3-28 20:24
Mélanie Meyer,Beno?t Masquida-government systems. We apply IGUAN framework [.] for usability investigation, which we developed, in our earlier research as a guideline for improving the usability of e-government systems. We constructed a scenario in usability investigation, a search for medical treatment. Our research follows a 作者: CRUC 時(shí)間: 2025-3-29 02:10
Elisa Biondi,Donald H. Burkele of extracting business process elements from unstructured texts. Process ontologies store these extracted elements and actual business process elements as well for providing a unified base to execute compliance checking investigation between actual and reference business processes.作者: 承認(rèn) 時(shí)間: 2025-3-29 06:05
Pablo Cordero,Wipapat Kladwang,Christopher C. VanLang,Rhiju Das user study has been conducted. The results show that trust-aware VAAs give satisfactorily accurate and helpful recommendations. Furthermore, causal relationships of the evaluated constructs demonstrate that by participating in forum discussions, a user can improve the accuracy of her/his recommenda作者: inhibit 時(shí)間: 2025-3-29 10:26
Nora Sachsenmaier,Stefan Handl,Franka Debeljak,Christina Waldsich user study has been conducted. The results show that trust-aware VAAs give satisfactorily accurate and helpful recommendations. Furthermore, causal relationships of the evaluated constructs demonstrate that by participating in forum discussions, a user can improve the accuracy of her/his recommenda作者: defuse 時(shí)間: 2025-3-29 12:58 作者: 哎呦 時(shí)間: 2025-3-29 19:16 作者: 重疊 時(shí)間: 2025-3-29 21:53 作者: 埋葬 時(shí)間: 2025-3-30 03:24 作者: 樣式 時(shí)間: 2025-3-30 04:43 作者: Demulcent 時(shí)間: 2025-3-30 10:43 作者: HARP 時(shí)間: 2025-3-30 12:33 作者: braggadocio 時(shí)間: 2025-3-30 18:46
Brant Gracia,Rick Russellts show that, among the models used, Convolutional Neural Network (CNN) is the best, with 95% accuracy and 0.91 F1-Score. Association results indicate that, among the rules generated, there is a high probability of drug law crimes leading to a dismissed habeas corpus (which means the maintenance of 作者: 推崇 時(shí)間: 2025-3-31 00:17
Katharina Semrad of collective knowledge, and also the networking collaboration. Considering the potential contributions of crowdstorming to promote innovations and improvements in the public sector and its potential to engage citizens, it is suggested that the project idea should be replicated in other parts of Br作者: miniature 時(shí)間: 2025-3-31 03:55
Sébastien H. Eschbach,Daniel A. Lafontaine of collective knowledge, and also the networking collaboration. Considering the potential contributions of crowdstorming to promote innovations and improvements in the public sector and its potential to engage citizens, it is suggested that the project idea should be replicated in other parts of Br作者: FLEET 時(shí)間: 2025-3-31 06:21
Somdeb MitraBusiness Process Modelling (BPM) and Semantic Annotation into existing SWS solutions as tools for the modeling and engineering of SWS for non-automated government operations and processes. The proposed infrastructure leverages SWS technology in e-government while enabling continuous re-engineering a作者: 方便 時(shí)間: 2025-3-31 11:53
Bishnu Paudel,David Rueda of collective knowledge, and also the networking collaboration. Considering the potential contributions of crowdstorming to promote innovations and improvements in the public sector and its potential to engage citizens, it is suggested that the project idea should be replicated in other parts of Br作者: lobster 時(shí)間: 2025-3-31 17:26 作者: Fretful 時(shí)間: 2025-3-31 17:58 作者: Musculoskeletal 時(shí)間: 2025-4-1 00:23
1064-3745 ng and avoiding known pitfalls..?.Practical and authoritative, .RNA Folding: Methods and Protocols. serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.978-1-4939-6058-3978-1-62703-667-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: gusher 時(shí)間: 2025-4-1 03:34 作者: Paleontology 時(shí)間: 2025-4-1 09:58
Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol, ligation efficiencies of ssDNA adapters, and avoiding problematic heuristics in prior algorithms. We hope that the step-by-step description of MAP-seq 1.0 will help other RNA mapping laboratories to transition from electrophoretic to next-generation sequencing methods and to further reduce the turn
