標題: Titlebook: RNA; Methods and Protocol Henrik Nielsen Book 2011 Springer Science+Business Media, LLC 2011 Bioinformatics.Mammalian genome.Messenger RNAs [打印本頁] 作者: Wilson 時間: 2025-3-21 16:16
書目名稱RNA影響因子(影響力)
書目名稱RNA影響因子(影響力)學(xué)科排名
書目名稱RNA網(wǎng)絡(luò)公開度
書目名稱RNA網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱RNA被引頻次
書目名稱RNA被引頻次學(xué)科排名
書目名稱RNA年度引用
書目名稱RNA年度引用學(xué)科排名
書目名稱RNA讀者反饋
書目名稱RNA讀者反饋學(xué)科排名
作者: Initial 時間: 2025-3-21 21:29 作者: Ambiguous 時間: 2025-3-22 04:27
Working with RNAk rules have to be implemented to maintain the integrity of the RNA. Alkaline pH, high temperatures, and heavy metal ions should be avoided when possible and ribonucleases kept in check. The chapter outlines the specific precautions recommended for work with RNA and describes some of the modificatio作者: antidepressant 時間: 2025-3-22 07:57
Synthesis of RNA by In Vitro Transcriptionto those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts 作者: 遺傳 時間: 2025-3-22 11:08
Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capturepoly(A). RNA directly from guanidinium thiocyanate (GuSCN)-containing cell or tissue extract by combining the design of biotinylated LNA oligo(T) capture probes with subsequent immobilization of the captured poly(A). RNA onto streptavidin-coated magnetic particles. In contrast to DNA oligo-dT and po作者: ATRIA 時間: 2025-3-22 15:39 作者: Axillary 時間: 2025-3-22 20:52 作者: muscle-fibers 時間: 2025-3-22 21:50 作者: interior 時間: 2025-3-23 01:47
Rapid Amplification of cDNA Ends (RACE)ly known. Starting with an mRNA mixture, gene-specific primers generated from the known regions of the gene and non-specific anchors, full-length sequences can be identified in as little as 3 days. RACE can also be used to identify alternative transcripts of a gene when the partial or complete seque作者: 整頓 時間: 2025-3-23 07:45
Fractionation of mRNA Based on the Length of the Poly(A) Taile mRNAs according to the length of the poly(A) tail. Poly(A) fractionation can be used to detect small changes in poly(A) tail length or to prepare samples for microarray analysis. RNA or crude lysate is mixed with biotinylated oligo(dT), which is then bound to paramagnetic streptavidin beads. Oligo作者: Prosaic 時間: 2025-3-23 12:30 作者: DEVIL 時間: 2025-3-23 16:45 作者: interior 時間: 2025-3-23 18:04
Promises and Challenges in Developing RNAi as a Research Tool and Therapyo, and many innovative screens have been reported on the use of RNAi to identify potential drug targets. Despite several technical advances, however, there are still many challenges in determining the ideal design of siRNA sequence, the activation of the immune system, off-target effects, and compet作者: languid 時間: 2025-3-23 23:11 作者: 異教徒 時間: 2025-3-24 04:49
The Post-transcriptional Operonroteins and/or small non-coding RNAs so that protein expression is coordinated at the post-transcriptional level. The post-transcriptional operon model (PTO) is used to describe data from an assortment of methods (e.g. RIP-Chip, CLIP-Chip, miRNA profiling, ribosome profiling) that globally address t作者: myocardium 時間: 2025-3-24 07:32
RIP-Chip Analysis: ,NA-Binding Protein ,mmuno,recipitation-Microarray (,) Profilinge expression through combinatorial binding to multiple, physically dispersed cis elements, mRNA binding proteins can regulate the translation of functionally related gene products by coordinately binding to subsets of mRNAs. The networks of mRNA binding proteins that facilitate this fine-tuning of g作者: Optimum 時間: 2025-3-24 11:24
Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Captureperatures compared to standard oligo(dT) technology. The successful use of the LNA oligo(T) capture method in recovery of mRNA from human cells and the subsequent use of the mRNA in northern blotting analysis, RT-PCR and qRT-PCR are demonstrated.作者: Tortuous 時間: 2025-3-24 16:43
The Transcriptional Landscapeependent transcription from within the unit. In genomic regions separating those that encode proteins or highly abundant RNA molecules with known function, transcripts are generally of low abundance and short-lived. In most of these cases, it is unclear to what extent a function is related to transcription . or to the RNA products.作者: FLAT 時間: 2025-3-24 20:18
Web-Based Tools for Studying RNA Structure and Function Here we present a comprehensive list of web based resources that can be used in the study and identification of RNA-based structural and regulatory motifs and provide a survey of the informatic resources that can have been developed to facilitate this research.作者: 煤渣 時間: 2025-3-25 01:05 作者: 胖人手藝好 時間: 2025-3-25 05:38 作者: 假 時間: 2025-3-25 08:05
Promises and Challenges in Developing RNAi as a Research Tool and Therapylving these challenges. This chapter summarizes recent progress in siRNA design, sensing by the immune system, and discusses some of the promising approaches that are currently being explored in separating siRNA unwanted effects from gene silencing.作者: 鞭子 時間: 2025-3-25 14:00 作者: emulsify 時間: 2025-3-25 18:22 作者: grotto 時間: 2025-3-25 23:00 作者: magenta 時間: 2025-3-26 02:05 作者: 改變 時間: 2025-3-26 06:02 作者: Sigmoidoscopy 時間: 2025-3-26 09:39
Genome Browsersshould be one of the very first steps taken in designing experiments. This can save considerable time in your research or as expressed by Alan Bleasby “Two months in the lab can easily save an afternoon on the computer.”作者: 1分開 時間: 2025-3-26 14:31 作者: Electrolysis 時間: 2025-3-26 20:31 作者: 圣歌 時間: 2025-3-27 00:22 作者: gratify 時間: 2025-3-27 02:53
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820144.jpg作者: 不知疲倦 時間: 2025-3-27 06:37 作者: glans-penis 時間: 2025-3-27 10:09
Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA)RNAi is now the preferred method for silencing gene expression in a variety of systems. In this chapter we describe the procedure for applying short-hairpin RNA (shRNA) to study gene function. Detailed descriptions of target site selection, shRNA construction, shRNA transfection and target knockdown validation are included.作者: 搜尋 時間: 2025-3-27 17:14 作者: 胖人手藝好 時間: 2025-3-27 20:06 作者: 勉勵 時間: 2025-3-28 00:12
(z.B. keine Reisekosten, Zeitersparnis) vor allem die Aktualit?t und die schnellere Informationsübermittlung betont. Business TV ist eine Multimedia-L?sung und ein Online-Informationsmedium. Im Unterschied zum ?ffentlichen Fernsehen, das an die Allgemeinheit gerichtet ist, dient BTV ?der Bearbeitung作者: Clinch 時間: 2025-3-28 03:14
Knud Josefsen,Henrik Nielseny different contributors. The author,together with colleague authors who provide valuable additionalexperience, presents examples of actual industrial world applications.Furthermore the academic concepts presented in this book provideexcellent theories to student readers and the concepts described a作者: maintenance 時間: 2025-3-28 08:50 作者: 真繁榮 時間: 2025-3-28 10:28
Hedda A. Meijer,Cornelia H. de Moory different contributors. The author,together with colleague authors who provide valuable additionalexperience, presents examples of actual industrial world applications.Furthermore the academic concepts presented in this book provideexcellent theories to student readers and the concepts described a作者: 埋葬 時間: 2025-3-28 16:35
Zhaiyi Zhang,Stefan Stamme products have also been used to vaporize other psychoactive substances, including cannabis products containing tetrahydrocannabinol (THC) and cannabidiol (CBD). In this chapter, we describe the basic structure of e-cigarettes and the significant differences among the different generations of produ作者: 廢除 時間: 2025-3-28 22:36 作者: intuition 時間: 2025-3-29 01:54 作者: chuckle 時間: 2025-3-29 06:24
Knud Josefsen,Ying C. Lees and develops them step-by-step into a broad range of applications of real circuits and systems..Written to be accessible to students of varying backgrounds, this textbook presents the design of realistic, working analog electronic circuits for key systems;.Includes worked examples of functioning c作者: kindred 時間: 2025-3-29 08:30
Joel Nelson,Oleg Denisenko,Karol Bomsztyks and develops them step-by-step into a broad range of applications of real circuits and systems..Written to be accessible to students of varying backgrounds, this textbook presents the design of realistic, working analog electronic circuits for key systems;.Includes worked examples of functioning c作者: gratify 時間: 2025-3-29 14:13
Scott A. Tenenbaum,Jan Christiansen,Henrik NielsenDs and FPGA s. Design examples and a short form guide for the digital synthesis toolispLever are included on the CD enclosed. Part II is dedicated to board level design. The main chapters of this part describe the use of operational ampli?ers for signal conditioning including signal ampli?cation, ?l作者: 下船 時間: 2025-3-29 16:43 作者: chastise 時間: 2025-3-29 23:23
Fractionation of mRNA Based on the Length of the Poly(A) Tail dependent on visualising the smears representing the variations in poly(A) tail length. The method is technically easy, fast, highly reproducible and can be performed on almost any sample containing RNA.作者: opalescence 時間: 2025-3-30 00:19 作者: 逃避系列單詞 時間: 2025-3-30 04:15 作者: Mercantile 時間: 2025-3-30 09:22 作者: 支架 時間: 2025-3-30 12:32 作者: 彩色 時間: 2025-3-30 17:55 作者: 戰(zhàn)勝 時間: 2025-3-30 22:40
Nana Jacobsen,Jens Eriksen,Peter Stein Nielsen’ oxidation and phase impurity, is demonstrated. Representing a unique reference guide, the book will be of considerable interest and value to members of the photoemission community engaged in solar cell research, and to a wider materials science audience as well.978-3-030-06276-7978-3-319-91665-1Series ISSN 2190-5053 Series E-ISSN 2190-5061 作者: BANAL 時間: 2025-3-31 03:05
Elfar Torarinsson’ oxidation and phase impurity, is demonstrated. Representing a unique reference guide, the book will be of considerable interest and value to members of the photoemission community engaged in solar cell research, and to a wider materials science audience as well.978-3-030-06276-7978-3-319-91665-1Series ISSN 2190-5053 Series E-ISSN 2190-5061 作者: Affectation 時間: 2025-3-31 05:36 作者: 啤酒 時間: 2025-3-31 11:41
J?rn Remi Henriksen,Jochen Buechner,Cecilie L?kke,Trond Fl?gstad,Christer Einvikystems..Written to be accessible to students of varying backgrounds, this textbook presents the design of realistic, working analog electronic circuits for key systems;.Includes worked examples of functioning c978-3-030-46991-7978-3-030-46989-4
