標題: Titlebook: Parasite Genomics Protocols; Sara E. Melville Book 2004 Humana Press 2004 [打印本頁] 作者: aggression 時間: 2025-3-21 18:55
書目名稱Parasite Genomics Protocols影響因子(影響力)
書目名稱Parasite Genomics Protocols影響因子(影響力)學科排名
書目名稱Parasite Genomics Protocols網絡公開度
書目名稱Parasite Genomics Protocols網絡公開度學科排名
書目名稱Parasite Genomics Protocols被引頻次
書目名稱Parasite Genomics Protocols被引頻次學科排名
書目名稱Parasite Genomics Protocols年度引用
書目名稱Parasite Genomics Protocols年度引用學科排名
書目名稱Parasite Genomics Protocols讀者反饋
書目名稱Parasite Genomics Protocols讀者反饋學科排名
作者: 煤渣 時間: 2025-3-21 23:53
Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics,acrylamide gel electrophoresis. These two methods are then integrated to allow the global changes in RNA and protein expression to be examined. The protocols have been adapted for working on parasites and contain extensive notes.作者: 遵循的規(guī)范 時間: 2025-3-22 03:34 作者: AUGUR 時間: 2025-3-22 07:58
1064-3745 te species is vital in attempts to avoid, prevent, or cure infection or to alleviate symptoms. Of course, the biological characteristics that each parasite has developed to aid survival and tra978-1-61737-331-2978-1-59259-793-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 壓倒 時間: 2025-3-22 10:25 作者: CHASE 時間: 2025-3-22 15:42
RACE and RAGE Cloning in Parasitic Microbial Eukaryotes, full-length mitochondrial Hsp70 could be cloned and sequenced and specific antibody raised against a fusion protein. The highly specific antibody allowed us to demonstrate for the first time the presence of mitochondrial-like organelles in microsporidia.作者: FLASK 時間: 2025-3-22 20:42 作者: Migratory 時間: 2025-3-22 22:57
Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments,from the reaction by . digestion, the pool of mutagenized plasmids can then be used directly in screening procedures..The different random mutagenesis strategies we describe should have wide applications in the production of libraries of large and diverse DNA libraries and in the generation of mutant proteins for structural and functional studies.作者: COLON 時間: 2025-3-23 04:02 作者: 粉筆 時間: 2025-3-23 05:47 作者: 喃喃而言 時間: 2025-3-23 11:08
Annotation of Parasite Genomes,e correspondingly different depths of annotation depending on what methods are employed. Researchers using genome data should, therefore, understand how annotations are generated to assess their validity correctly and to determine what level of inferences can be made accordingly. Thorough annotation作者: 無法破譯 時間: 2025-3-23 17:25 作者: Oration 時間: 2025-3-23 20:40
Expressed Sequence Tags, a single-pass sequence derived from a single complementary DNA (cDNA) clone, and the sequence serves to identify the gene from which it derives. We present a set of tested laboratory protocols for setting up and performing an EST analysis of any chosen species. These medium-throughput protocols do 作者: ENDOW 時間: 2025-3-23 23:18
Expressed Sequence Tags,have significant internal redundancy. To facilitate analysis of small EST datasets from in-house projects, we present an integrated “pipeline” of tools that take EST data from sequence trace to database submission. These tools also can be used to provide clustering of ESTs into putative genes and to作者: 輪流 時間: 2025-3-24 03:31
Positive Selection Scanning of Parasite DNA Sequences,tive mutations can provide a guide to biological processes that are potentially essential to the parasite. Random genetic mutations that confer selective advantage can act to alter amino acids so as to confer gain of function that has positive impact on the survival of the parasite. Directional sele作者: Munificent 時間: 2025-3-24 09:05 作者: 的’ 時間: 2025-3-24 13:17
Amplified (Restriction) Fragment Length Polymorphism (AFLP) Analysis,sity among various organisms with varying genomic complexity, from small bacterial to large plant genomes. AFLP analysis combines the reliability of restriction enzyme digestion with the utility of the polymerase chain reaction. The technique can be applied to studies of DNA of any origin and comple作者: 無辜 時間: 2025-3-24 18:09 作者: 鋸齒狀 時間: 2025-3-24 20:35 作者: conception 時間: 2025-3-25 02:57
Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays,ns to study transcriptional processes in a systematic and high-throughput manner. Specifically in this chapter, we outline the ordered processes involved in large-scale parasite gene expression studies including complementary (cDNA) microarray fabrication, total RNA isolation, cDNA labeling using fl作者: hurricane 時間: 2025-3-25 06:10 作者: deactivate 時間: 2025-3-25 09:54 作者: 開頭 時間: 2025-3-25 14:01
Transfection of the Human Malaria Parasite ,,placement, and transgene expression in this organism. These methods are detailed in this chapter, as are approaches used to monitor transfectants during the selection process. The different plasmid vectors that are currently used for gene targeting and transgene expression (including green fluoresce作者: Fracture 時間: 2025-3-25 16:02
A PCR-Based Method for Gene Deletion and Protein Tagging in ,,. Here, we describe a polymerase chain reaction (PCR)-based method for direct gene deletion and the generation of epitope-tagged fusion proteins. The approach is based on methodologies developed for . and involves PCR amplification of a reporter cassette using primers containing flanking sequences s作者: Ophthalmoscope 時間: 2025-3-25 22:16 作者: 禁止 時間: 2025-3-26 02:21
In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons,ch for gene identification and analysis is transposon mutagenesis. This can be performed directly in vivo, but often it is more convenient to generate transpositions in vitro for subsequent analysis in vivo, in a process termed “shuttle mutagenesis.” The . element . is well suited for application by作者: dendrites 時間: 2025-3-26 06:26
Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments,s strategy. This chapter describes three different methods for random mutagenesis, the use of XL1-red cells, error-prone polymerase chain reaction (PCR), and degenerate oligonucleotides-. (DOP). These mutagenesis strategies possess different benefits and pitfalls; thus, they are differentially usefu作者: 外來 時間: 2025-3-26 11:14
Separation, Digestion, and Cloning of Intact Parasite Chromosomes Embedded in Agarose,ntly at metaphase. Therefore, the development of pulsed field gel electrophoresis allowed the resolution of many parasite karyotypes for the first time. The ability to prepare intact chromosomes in agarose plugs and to isolate individual homologs by electrophoresis has led to many new applications i作者: evaculate 時間: 2025-3-26 13:05 作者: Project 時間: 2025-3-26 18:18 作者: GRIPE 時間: 2025-3-27 00:28 作者: GULF 時間: 2025-3-27 01:29
Claire Whitton,Jennifer Daub,Marian Thompson,Mark Blaxterliegende Nachschlagewerk eignet sich für den ersten schnellen überblick. In 440 übersichtlichen Schlüsselbegriffen werden unter anderem grundlegende Konzepte, Institutionen und Abkommen erl?utert. Die Erkl?rungen sind kompakt und verst?ndlich formuliert und bieten Basiswissen für alle, die einen sch作者: Expertise 時間: 2025-3-27 06:50 作者: 埋葬 時間: 2025-3-27 09:29
Winston A. Hide,Raphael D. Isokpehiliegende Nachschlagewerk eignet sich für den ersten schnellen überblick. In 440 übersichtlichen Schlüsselbegriffen werden unter anderem grundlegende Konzepte, Institutionen und Abkommen erl?utert. Die Erkl?rungen sind kompakt und verst?ndlich formuliert und bieten Basiswissen für alle, die einen sch作者: 用樹皮 時間: 2025-3-27 15:36
Bryony A. P. Williams,Robert P. Hirtliegende Nachschlagewerk eignet sich für den ersten schnellen überblick. In 440 übersichtlichen Schlüsselbegriffen werden unter anderem grundlegende Konzepte, Institutionen und Abkommen erl?utert. Die Erkl?rungen sind kompakt und verst?ndlich formuliert und bieten Basiswissen für alle, die einen sch作者: 團結 時間: 2025-3-27 21:26
