標題: Titlebook: Methods of Protein Microcharacterization; A Practical Handbook John E. Shively Book 1986 Springer Science+Business Media LLC 1986 amino aci [打印本頁] 作者: 閃爍 時間: 2025-3-21 19:17
書目名稱Methods of Protein Microcharacterization影響因子(影響力)
書目名稱Methods of Protein Microcharacterization影響因子(影響力)學科排名
書目名稱Methods of Protein Microcharacterization網(wǎng)絡公開度
書目名稱Methods of Protein Microcharacterization網(wǎng)絡公開度學科排名
書目名稱Methods of Protein Microcharacterization被引頻次
書目名稱Methods of Protein Microcharacterization被引頻次學科排名
書目名稱Methods of Protein Microcharacterization年度引用
書目名稱Methods of Protein Microcharacterization年度引用學科排名
書目名稱Methods of Protein Microcharacterization讀者反饋
書目名稱Methods of Protein Microcharacterization讀者反饋學科排名
作者: 放逐某人 時間: 2025-3-21 22:09
978-1-4899-4132-9Springer Science+Business Media LLC 1986作者: Host142 時間: 2025-3-22 04:16 作者: 敲詐 時間: 2025-3-22 05:03 作者: carotid-bruit 時間: 2025-3-22 11:46 作者: 受傷 時間: 2025-3-22 16:00
Michael W. Hunkapiller,Kristina Granlund-Moyer,Norman W. Whiteley作者: Cardiac 時間: 2025-3-22 19:56 作者: Efflorescent 時間: 2025-3-22 23:41
pre-embedding immunogold method, which uses a small-sized gold particle. The pre-embedding immunoperoxidase technique provides valuable information on the regional distribution of proteins of relevance for neurotransmission, while the pre-embedding immunogold technique is reliable for the localizati作者: 治愈 時間: 2025-3-23 04:42
Petro E. Petridesg brains. While this technique lacks cellular resolution, it provides high sensitivity and much improved consistency compared to conventional immunohistochemical techniques, which is essential for reliable quantitative comparisons of overall expression levels of proteins in different brain regions. 作者: 半球 時間: 2025-3-23 09:23 作者: llibretto 時間: 2025-3-23 12:26 作者: 變形 時間: 2025-3-23 15:37 作者: Glaci冰 時間: 2025-3-23 18:14
Yu-Ching E. Pan,Stanley Stein and the existence of recep- tor-ligand complexes at highest density in coated pits define the process as selective and concentrative. This topic has received ever increasing attention during the past few years. The evolving mechanisms are especially exciting because they come at a time when the con作者: nocturnal 時間: 2025-3-24 01:39 作者: COUCH 時間: 2025-3-24 03:12
PAGE under non-reducing conditions. We have recently described a new method for the detection of bound lipoproteins based on the high affinity streptavidin-biotin interaction (Wade ., 1985), and in this paper we report that a protein of MW approx. 130,000–150,000 can be detected in extracts of both 作者: DUCE 時間: 2025-3-24 06:56
Michael W. Hunkapiller,Chin-Sook Kimy fusing the fragments to two interacting proteins. The advantage of BiFC over alternative resonance energy transfer techniques is a high signal-to-noise ratio due to its strong intrinsic fluorescence without exogenous fluorogenic or chromogenic agents required. Here we provide a detailed descriptio作者: INERT 時間: 2025-3-24 11:25 作者: 羞辱 時間: 2025-3-24 15:59
Amino Acid Analysis With Postcolumn Fluorescent Derivatization primary method utilizes separation of the amino acids on a cation-exchange column, followed by postcolumn reaction with fluorescamine (.–.). This methodology will be presented in this article, but it will be placed in perspective with respect to the other approaches for amino acid analysis.作者: CODA 時間: 2025-3-24 21:14 作者: EVICT 時間: 2025-3-24 23:34
Comparison of the Spinning Cup and Gas-Phase Instrumentso sequence low nanomolar to subnanomolar amounts of sample. The use of polybrene film to immobilize the sample was critical to the methodology, and was first introduced by Tarr (.). As demonstrated by Wittmann-Liebold (.), rigorous purification of reagents and solvents is also important for improved作者: penance 時間: 2025-3-25 04:58 作者: Anemia 時間: 2025-3-25 10:14 作者: Infantry 時間: 2025-3-25 15:35 作者: 提名的名單 時間: 2025-3-25 17:37
Analysis of Phenylthiohydantoin Amino Acids by HPLC. It is the only technique commonly used for PTH analysis that can resolve all of the PTH amino acids in a single chromatographic run. Because it provides quantitative data at the pmol level, HPLC is also the only available method suitable for microsequencing that uses the new generation of automated Edman sequencers (.,.).作者: Interregnum 時間: 2025-3-25 22:19 作者: initiate 時間: 2025-3-26 01:47
Manual Edman Sequencing Systementific discipline, existing as it does merely to supply probes to the molecular geneticists and occasionally to check their DNA reading frames, and one has a method in deep psychological crisis. Fortunately, manual sequencing can provide any of the structural data that requires or advantageously utilizes protein chemistry.作者: 寬大 時間: 2025-3-26 06:27
Gas-Phase Protein/Peptide Sequencer). The resulting amino acid derivative-an anilinothiazolinone (ATZ)- is converted to the more stable phenylthiohydantoin (PTH) amino acid, which is analyzed by high performance liquid chromatography (HPLC). The remaining peptide is subjected to further degradation cycles to generate its amino acid sequence.作者: Genome 時間: 2025-3-26 09:47
Design of a Multipurpose Sequencer-called liquid-phase technique, in which the protein is degraded as thin film in a spinning cup, has been widely used and successfully applied to the sequence determination of proteins and polypeptide fragments.作者: nitroglycerin 時間: 2025-3-26 15:55 作者: 先驅(qū) 時間: 2025-3-26 18:00
COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methodsrminal modifications, especially for many regulatory pep-tides (.,.). Thus, whenever Edman degradation is used as the prinicpal method of complete amino acid sequence determination, it must be supplemented by COOH-terminal analysis.作者: arousal 時間: 2025-3-26 21:08 作者: 難取悅 時間: 2025-3-27 04:48 作者: 薄荷醇 時間: 2025-3-27 06:25 作者: 全國性 時間: 2025-3-27 13:10
Amino Acid Analysis With Postcolumn Fluorescent Derivatizationbut also for confirming the results from other types of analyses. Most current commercial analyzers are essentially modernized versions of the original autoanalyzer of Spackman, Stein, and Moore (.), designed some 30 yr ago. However, other methodologies for amino acid analysis are now becoming more 作者: radiograph 時間: 2025-3-27 17:38 作者: 帶來墨水 時間: 2025-3-27 19:58 作者: Override 時間: 2025-3-28 00:26
Comparison of the Spinning Cup and Gas-Phase Instrumentscer became the workhorse of protein chemists. The nominal operating range of the Beckman instrument was 100–500 nmol of protein. With the exception of a few minor changes, the Beckman instrument has remained largely unchanged over a period of 15 yr. Major changes in the reagent/solvent delivery syst作者: Uncultured 時間: 2025-3-28 02:52
Gas-Phase Protein/Peptide Sequenceructure of proteins and peptides (.). This chemical process is a repetitive reaction in which the alpha-amino group of the polypeptide chain is coupled to phenylisothiocyanate (PITC) under alkaline conditions, whereupon the terminal amino acid is cleaved from the remainder of the peptide with acid (.作者: 織布機 時間: 2025-3-28 09:27
Design of a Multipurpose Sequencerthe effectiveness of the sequential degradations, increased the repetitive yields, lowered the background contaminations of the released amino acid derivatives, and allowed considerably extended degradation of polypeptides in comparison to any of the manual techniques available at that time. This so作者: MELON 時間: 2025-3-28 10:48 作者: 遭遇 時間: 2025-3-28 17:58 作者: 法律的瑕疵 時間: 2025-3-28 19:52 作者: MOCK 時間: 2025-3-29 00:23 作者: neuron 時間: 2025-3-29 03:45
COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methodsvolume), this cannot be achieved by Edman degradation because this stepwise procedure does not allow discrimination between polypeptides with free or blocked COOH-terminal carboxylic groups. This shortcoming of Edman degradation must not be ignored in view of the significant biologic role of COOH-te作者: 闖入 時間: 2025-3-29 10:35
nsights into the structure-function of neurotransmitter receptors and ion channels in the brain. These techniques involve immunoreactions with antibodies before embedding and ultrathin sectioning. As a result, they are very sensitive and show excellent resolution and good ultrastructural and antigen作者: ARM 時間: 2025-3-29 14:56
nsights into the structure-function of neurotransmitter receptors and ion channels in the brain. These techniques involve immunoreactions with antibodies before embedding and ultrathin sectioning. As a result, they are very sensitive and show excellent resolution and good ultrastructural and antigen作者: Commission 時間: 2025-3-29 19:34
Petro E. Petrides without compromising the integrity of antibody-binding sites by tissue fixation, which is required for conventional immunocytochemistry. Fixation introduces covalent modifications, cross-linking and/or denaturation of proteins. These chemical modifications often alter the antibody-binding sites, an作者: CLAM 時間: 2025-3-29 21:31
John E. Shivelyins. For this purpose, expression vectors based on Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus, all members of the alphavirus genus, have been engineered in the form of naked RNA replicons, recombinant viral particles, and layered DNA vectors. By applying these alph作者: transplantation 時間: 2025-3-30 00:39 作者: indignant 時間: 2025-3-30 07:01 作者: defuse 時間: 2025-3-30 10:50
Yu-Ching E. Pan,Stanley Stein process, cell surface receptors specifically bind protein ligands and localize them to specialized invaginations of the plasma membrane. These regions are coated pits, so named because they are lined on the cytoplasmic face with an ordered array of the protein, clathrin. It is this ‘coat‘ which pro作者: Embolic-Stroke 時間: 2025-3-30 14:47
Barry Nelson Jonests to apply this concept to the elimination of neoplastic and other target cells using antibodies coupled to toxic agents. A cell-binding antibody conjugated to a plant or bacterial toxin has been termed an “immunotoxin”. One such toxin, ricin, like most toxic proteins produced by bacteria and plant作者: 橫截,橫斷 時間: 2025-3-30 17:53 作者: 細頸瓶 時間: 2025-3-31 00:41
