標(biāo)題: Titlebook: Memory B-Cells; Methods and Protocol Kim L. Good-Jacobson Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licens [打印本頁(yè)] 作者: legerdemain 時(shí)間: 2025-3-21 19:33
書(shū)目名稱Memory B-Cells影響因子(影響力)
作者: 微枝末節(jié) 時(shí)間: 2025-3-21 23:23 作者: opportune 時(shí)間: 2025-3-22 03:32 作者: 仇恨 時(shí)間: 2025-3-22 07:31 作者: SLAY 時(shí)間: 2025-3-22 10:21
Eden S. Hirsch,Jason S. Weinsteinert systems....This book was awarded the first DeGroot Prize by the International Society for Bayesian Analysis for a book making an important, timely, thorough, and notably original contribution to the statistics literature..978-0-387-71823-1978-0-387-22630-9Series ISSN 1613-9011 Series E-ISSN 2197-4128 作者: Diverticulitis 時(shí)間: 2025-3-22 16:08
Ariel Simpson,Sarah A. Jones,Kirsten A. Fairfaxert systems....This book was awarded the first DeGroot Prize by the International Society for Bayesian Analysis for a book making an important, timely, thorough, and notably original contribution to the statistics literature..978-0-387-71823-1978-0-387-22630-9Series ISSN 1613-9011 Series E-ISSN 2197-4128 作者: lymphedema 時(shí)間: 2025-3-22 19:31 作者: 事物的方面 時(shí)間: 2025-3-23 00:34 作者: 責(zé)問(wèn) 時(shí)間: 2025-3-23 03:26 作者: Nonflammable 時(shí)間: 2025-3-23 08:05 作者: lambaste 時(shí)間: 2025-3-23 09:45
Book 2024tory protocols, and key tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Memory B-Cells.: Methods and Protocols .aims to ensure successful results in the further study of this vital field..作者: Rustproof 時(shí)間: 2025-3-23 17:43 作者: 聽(tīng)覺(jué) 時(shí)間: 2025-3-23 18:13 作者: 不公開(kāi) 時(shí)間: 2025-3-23 23:05 作者: 徹底明白 時(shí)間: 2025-3-24 03:15
Clarissa R. Chakma,Andrew Garvie,Lee H. Wong,Kim L. Good-Jacobson作者: AROMA 時(shí)間: 2025-3-24 08:58
Bagdeser Akdogan-Ozdilek,Lou-Ella M. M. George-Alexander,Christopher D. Scharer作者: 陰謀 時(shí)間: 2025-3-24 13:11 作者: intrigue 時(shí)間: 2025-3-24 18:43 作者: 戲法 時(shí)間: 2025-3-24 19:47
Methods in Molecular Biologyhttp://image.papertrans.cn/m/image/630462.jpg作者: gusher 時(shí)間: 2025-3-25 02:04 作者: Outmoded 時(shí)間: 2025-3-25 05:17 作者: 兩種語(yǔ)言 時(shí)間: 2025-3-25 10:46 作者: Camouflage 時(shí)間: 2025-3-25 14:20
https://doi.org/10.1007/978-1-0716-3950-4Secondary pathogen exposure; Vaccine-induced immune memory; Antigen-specific memory B cells; Tetramers; 作者: 的’ 時(shí)間: 2025-3-25 17:31 作者: 牙齒 時(shí)間: 2025-3-25 23:43
In Vivo CRISPR/Cas9-Mediated Gene Ablation in Murine B Cellserentiation of germinal center B cells. Here, we describe an approach to perform an in vivo Crispr-Cas9 screen to specifically ablate genes in activated B cells. Using this approach, we have been able to reveal novel transcriptional regulators of germinal center B cell differentiation following viral infection.作者: MELD 時(shí)間: 2025-3-26 00:39 作者: Malaise 時(shí)間: 2025-3-26 05:06 作者: Forsake 時(shí)間: 2025-3-26 10:09 作者: Friction 時(shí)間: 2025-3-26 12:55
Using Ex Vivo Tonsil Organoids to Study Memory B Cellsvidual variation in response to a variety of stimuli. Lymphoid tissue organoids enable characterization of pre-existing antigen-specific MBCs within an entirely human system and can provide valuable insights into MBC dynamics.作者: rectocele 時(shí)間: 2025-3-26 18:51
Flow Cytometric Identification of Human IgE+ B Lineage Subsetse used to robustly validate this approach before application to primary human samples. Orthogonal confirmatory techniques remain essential; these are not described in detail, but several possible strategies are suggested.作者: 清真寺 時(shí)間: 2025-3-27 00:41
Identification of IgG1 and IgG3 Allotypes by PCR and Sanger Sequencing(SNPs) responsible for the observed amino acid substitutions. Here, we provide a detailed protocol for the amplification of . and . segments by PCR, sample preparation for Sanger sequencing, and analysis of sequencing data to identify SNPs associated with different IgG1 and IgG3 allotypes.作者: Obstruction 時(shí)間: 2025-3-27 01:59 作者: Ganglion 時(shí)間: 2025-3-27 05:46
Visualizing Epigenetic Modifications and Nuclear Bodies by Immunofluorescence Staining in Na?ve, Acte elucidation of their function. Here, we provide a protocol for immunofluorescence staining in the nucleus, which has successfully been used to visualize histone modifications and nuclear bodies in human and mouse B lymphocytes, using as few as 1?×?10.–5?×?10. cells.作者: circumvent 時(shí)間: 2025-3-27 13:30
Measuring B Cell Chromatin Accessibility by One-Step ATAC-seqted and adapter tagged DNA is then purified and PCR amplified with dual indexing primers to generate a size-specific sequencing library. The One-Step workflow below outlines the Tn5 nuclei transposition from a range of cell inputs followed by PCR amplification to generate a sequencing library.作者: 橫條 時(shí)間: 2025-3-27 16:31 作者: 里程碑 時(shí)間: 2025-3-27 21:34 作者: Biguanides 時(shí)間: 2025-3-27 22:52 作者: Fulsome 時(shí)間: 2025-3-28 02:34 作者: AVERT 時(shí)間: 2025-3-28 09:22
Ultrasound-Guided Fine Needle Biopsy of Human Axillary Lymph Nodes to Assess B Cell Responses to Vacthe immune response within the lymph nodes following vaccination. Once acquired, lymph node cells can be characterized via flow cytometric immunophenotyping and/or single-cell RNA sequencing for gene expression and T and B cell receptors. Analysis of the immune cells from the lymph nodes enables the作者: malapropism 時(shí)間: 2025-3-28 12:57 作者: 尾巴 時(shí)間: 2025-3-28 17:05 作者: 欄桿 時(shí)間: 2025-3-28 21:42 作者: mosque 時(shí)間: 2025-3-29 01:06
Epigenomic Profiling of B Cell Subsets by CUT&Tag mechanisms, such as the post-translational modifications to histones, present several technical challenges that prevent a complete understanding of gene regulation. Here, we provide a detailed protocol of the Cleavage Under Targets and Tagmentation (CUT&Tag) chromatin profiling technique for identi作者: GROWL 時(shí)間: 2025-3-29 04:02 作者: Exclaim 時(shí)間: 2025-3-29 09:56
Isolation of Rare Antigen-Specific Memory B Cells via Antigen Tetramerseeper understanding of the mechanisms underlying this phenomenon can aide in research aimed to improve vaccines and therapies. Memory B cells (MBCs) are a fundamental component of immunological memory but can exist in rare populations that prove challenging to study. By combining fluorescent antigen作者: CUB 時(shí)間: 2025-3-29 13:29 作者: GLUE 時(shí)間: 2025-3-29 19:19
ELISpots for Detecting Antigen-Specific Memory B Cells in Infection or Autoimmunitycells cultured in ELISpot plates (96- or 8-well format) bind to a specific antigen bound to a PVDF membrane at the bottom of the well. A detection antibody followed by an enzymatic reaction is used to identify secreted protein bound to the membrane coated antigen. This reaction results in distinct “作者: 鞭子 時(shí)間: 2025-3-29 23:00 作者: SPECT 時(shí)間: 2025-3-30 00:11 作者: 其他 時(shí)間: 2025-3-30 08:07 作者: faction 時(shí)間: 2025-3-30 12:17
Identification of IgG1 and IgG3 Allotypes by PCR and Sanger Sequencingiation along the antibody constant heavy chain referred to as allotypes. . and . are the two most polymorphic IgG subclasses in humans, with 4 classical IgG1 allotypes and 13 allotypes described for IgG3, though recent studies suggest greater allelic diversity, especially in underrepresented ethnic 作者: 仲裁者 時(shí)間: 2025-3-30 15:39 作者: 褲子 時(shí)間: 2025-3-30 19:55
Eden S. Hirsch,Jason S. Weinsteinsystems are graphical networks that support the modelling of uncertainty and decisions in large complex domains, while retaining ease of calculation. Building on original research by the authors over a number of years, this book gives a thorough and rigorous mathematical treatment of the underlying 作者: Wallow 時(shí)間: 2025-3-30 20:57
Ariel Simpson,Sarah A. Jones,Kirsten A. Fairfaxsystems are graphical networks that support the modelling of uncertainty and decisions in large complex domains, while retaining ease of calculation. Building on original research by the authors over a number of years, this book gives a thorough and rigorous mathematical treatment of the underlying 作者: achlorhydria 時(shí)間: 2025-3-31 03:59
systems are graphical networks that support the modelling of uncertainty and decisions in large complex domains, while retaining ease of calculation. Building on original research by the authors over a number of years, this book gives a thorough and rigorous mathematical treatment of the underlying 作者: 寬宏大量 時(shí)間: 2025-3-31 07:40