標(biāo)題: Titlebook: Light Microscopy; Methods and Protocol Yolanda Markaki,Hartmann Harz Book 2017 Springer Science+Business Media LLC 2017 confocal laser scan [打印本頁] 作者: 啞劇表演 時間: 2025-3-21 16:43
書目名稱Light Microscopy影響因子(影響力)
書目名稱Light Microscopy影響因子(影響力)學(xué)科排名
書目名稱Light Microscopy網(wǎng)絡(luò)公開度
書目名稱Light Microscopy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Light Microscopy被引頻次
書目名稱Light Microscopy被引頻次學(xué)科排名
書目名稱Light Microscopy年度引用
書目名稱Light Microscopy年度引用學(xué)科排名
書目名稱Light Microscopy讀者反饋
書目名稱Light Microscopy讀者反饋學(xué)科排名
作者: CRAFT 時間: 2025-3-21 22:11
Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenere imaging, and multi-day window imaging. We carefully compare and explain in depth how to set up each method. Lastly, in the notes section we mention some alternative solutions for the 2P-IVM methods described. In conclusion, this protocol can be used as a guide towards deciding which 2P-IVM method to use and to enable the setup of this method.作者: Fluctuate 時間: 2025-3-22 02:53 作者: 格言 時間: 2025-3-22 07:25
Book 2017sed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging f作者: Stable-Angina 時間: 2025-3-22 10:37 作者: 最高點 時間: 2025-3-22 14:10 作者: 變白 時間: 2025-3-22 19:38
Introduction to Modern Methods in Light Microscopy,n of modern microscopy techniques. We briefly discuss the basics of optics ., super-resolution imaging, quantitative image analysis, live-cell imaging, and provide an outlook on active research areas pertaining to light microscopy.作者: Protein 時間: 2025-3-22 23:35
FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cellsted in intact cells or tissues and even in various subcellular compartments. Here, we describe how to perform FRET measurements in living cells expressing FRET-based biosensors and how to evaluate these data. This general protocol can be applied for FRET measurements with various fluorescent biosensors.作者: MAPLE 時間: 2025-3-23 04:12 作者: AUGUR 時間: 2025-3-23 08:04 作者: Commemorate 時間: 2025-3-23 12:47
STED Imaging in , Brain Slicesimmunolabeling and imaging with STED microscopy. This protocol allowed us to image small dendritic branches from neurons located deep in the fly brain with improved resolution compared with conventional light microscopy.作者: CARK 時間: 2025-3-23 15:39 作者: 懸崖 時間: 2025-3-23 20:11 作者: 最高峰 時間: 2025-3-24 01:01 作者: Conscientious 時間: 2025-3-24 03:59
Erinke van Grinsven,Chloé Prunier,Nienke Vrisekoop,Laila Ritsmaeory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o作者: 容易生皺紋 時間: 2025-3-24 06:57
Peter Quicke,Samuel J. Barnes,Thomas Kn?pfeleory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o作者: Abjure 時間: 2025-3-24 12:13 作者: Prostaglandins 時間: 2025-3-24 15:57
David Domozych,Anna Lietz,Molly Patten,Emily Singer,Berke Tinaz,Sandra C. Raimundoions of crystallographic statistics, the theory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o作者: MUTE 時間: 2025-3-24 22:53 作者: Capture 時間: 2025-3-25 02:19 作者: IOTA 時間: 2025-3-25 06:42 作者: 支柱 時間: 2025-3-25 09:00
Sandra Fendl,Jesús Pujol-Martí,Joel Ryan,Alexander Borst,Robert Kaspereory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o作者: Abbreviate 時間: 2025-3-25 12:12 作者: 總 時間: 2025-3-25 19:27
Rainer A. Leitgebions of crystallographic statistics, the theory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o作者: laparoscopy 時間: 2025-3-25 23:09 作者: Nutrient 時間: 2025-3-26 02:01
Ignacio Arganda-Carreras,Philippe Andreyeory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o作者: flutter 時間: 2025-3-26 07:21
Yao Yao,Ihor Smal,Ilya Grigoriev,Maud Martin,Anna Akhmanova,Erik Meijeringeory of direct methods, Patterson techniques, isomorphous replacement and anomalous scattering, and treatments of the role of electron microscopy and diffraction in crystal structure determination, including applications of direct methods to electron crystallography. Part 3 deals with applications o作者: 正論 時間: 2025-3-26 09:04 作者: 巧思 時間: 2025-3-26 15:06 作者: Creditee 時間: 2025-3-26 19:35
Designing Image Analysis Pipelines in Light Microscopy: A Rational Approachch is to follow an iterative, backwards procedure from the target objectives of analysis. The proposed goal-oriented strategy should help biologists to better apprehend image analysis in the context of their research and should allow them to efficiently interact with image processing specialists.作者: 焦慮 時間: 2025-3-26 21:19
Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP)analysis of protein kinetics using FRAP. We focus on the most commonly encountered challenges and technical or computational pitfalls and their troubleshooting so that valid and robust insight into protein dynamics within living cells is gained.作者: archenemy 時間: 2025-3-27 03:33 作者: Debrief 時間: 2025-3-27 05:23 作者: Vasodilation 時間: 2025-3-27 12:56 作者: 榨取 時間: 2025-3-27 14:51
Francesco Pampaloni,Laura Knuppertz,Andrea Hamann,Heinz D. Osiewacz,Ernst H. K. Stelzerscientists who employ crystallographic methods and who are concerned with the structure and the properties of crystalline materials...The fifth edition of Volume A has been reviewed by P. Paufler [Acta Cryst. (作者: observatory 時間: 2025-3-27 21:33 作者: 邊緣 時間: 2025-3-27 23:13
https://doi.org/10.1007/978-1-4939-6810-7confocal laser scanning and spinning disk confocal microscopy; Fluorescence Resonance Energy Transfer作者: 荒唐 時間: 2025-3-28 03:21
978-1-4939-8305-6Springer Science+Business Media LLC 2017作者: 游行 時間: 2025-3-28 09:34 作者: Perigee 時間: 2025-3-28 13:07
Yolanda Markaki,Hartmann HarzIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: incarcerate 時間: 2025-3-28 15:43 作者: Axillary 時間: 2025-3-28 20:19 作者: 熱心 時間: 2025-3-29 00:44
Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model .. The protocol explains the mounting of the live fungi for the light sheet imaging, the imaging procedure and illustrates basic image processing of data.作者: 粗野 時間: 2025-3-29 04:09
Imaging of Brain Slices with a Genetically Encoded Voltage Indicatorring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.作者: hypnotic 時間: 2025-3-29 08:43 作者: constitute 時間: 2025-3-29 14:22 作者: 夸張 時間: 2025-3-29 16:41
Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model .. The protocol explains the mounting of the live fungi for the light sheet imaging, the imaging procedure a作者: Inferior 時間: 2025-3-29 22:20
Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopytworks and whole organs in three dimensions. Because this technique requires specimens to be translucent they commonly have to be cleared before microscopy inspection. Here, we provide 3DISCO based protocols for preparing cleared samples of immuno-stained neural networks, lectin-labeled vascular net作者: 噱頭 時間: 2025-3-30 01:08 作者: ellagic-acid 時間: 2025-3-30 06:23 作者: frugal 時間: 2025-3-30 12:08 作者: CRATE 時間: 2025-3-30 16:21
Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, ,contains several polymers that are highly similar to those found in the primary cell walls of land plants. . is easily grown in laboratory culture and is effectively manipulated in various experimental protocols including microplate assays and correlative microscopy. Most importantly, . can be live 作者: apropos 時間: 2025-3-30 19:09
Targeted Ablation Using Laser Nanosurgerythis technique more precise, powerful, and easy to use. Today pulsed lasers can be operated with diffraction limited, sub-micrometer precision to ablate intracellular structures. Here, we discuss laser nanosurgery setups and the instrumentation in our laboratory. We describe how to use this techniqu作者: 密切關(guān)系 時間: 2025-3-31 00:00 作者: 負(fù)擔(dān) 時間: 2025-3-31 01:11
STED Imaging in , Brain Slicesance, stimulated emission depletion (STED) microscopy has been successfully used in recent years to investigate the arrangement and colocalization of different protein species in cells in culture and on the surface of specimens. However, because of its extreme sensitivity to light scattering, super-作者: 間諜活動 時間: 2025-3-31 05:49
Optical Coherence Microscopye review the functional imaging capabilities of OCM focusing on lable-free optical angiography. We conclude with a section on digital wavefront control and a short outlook on future developments, in particular for contrast enhancement techniques.作者: mosque 時間: 2025-3-31 12:42 作者: obstinate 時間: 2025-3-31 13:43
Automated Analysis of Intracellular Dynamic Processesand therapies. Advanced fluorescence microscopy imaging systems nowadays allow the recording of virtually any type of process in space and time with super-resolved detail and with high sensitivity and specificity. The large volume and high information content of the resulting image data, and the des作者: PUT 時間: 2025-3-31 21:32
Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cellse gene of interest. The resulting reporter transgene is then integrated in the genome of cells that express the phage protein fused to a fluorescent tag. Upon transcription, binding of the fluorescent protein to its target sequence makes the RNA visible. With this approach it is possible to track, i作者: Cardioplegia 時間: 2025-4-1 00:16
