標(biāo)題: Titlebook: Ion Channels; Methods and Protocol James D. Stockand,Mark S. Shapiro Book 2006 Humana Press 2006 Escherichia coli.Lipid.Vivo.molecular biol [打印本頁] 作者: 自由 時(shí)間: 2025-3-21 19:52
書目名稱Ion Channels影響因子(影響力)
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書目名稱Ion Channels網(wǎng)絡(luò)公開度
書目名稱Ion Channels網(wǎng)絡(luò)公開度學(xué)科排名
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作者: 生存環(huán)境 時(shí)間: 2025-3-21 20:13
und Rohrleitungsbaus ein.Enth?lt ein spezielles Kapitel überDieses Fachbuch vermittelt wesentliche Grundlagen für die Entwicklung und Planung verfahrenstechnischer Anlagen, bei der technische, betriebliche, wirtschaftliche und rechtliche Forderungen beachtet werden müssen. Nach einer Einführung in d作者: 沉積物 時(shí)間: 2025-3-22 00:50
Alexander Staruschenko,Rachell E. Booth,Oleh Pochynyuk,James D. Stockand,Qiusheng Tongen Vorschriften, die insbesondere Sicherheitsanforderungen betreffen, beachtet werden. Meist sind verschiedene L?sungen m?glich. Die Dimensionierung und Gestaltung von Elementen des Apparate- und Maschinenbaues ist Gegenstand eigenst?ndiger Fachdisziplinen mit einer umfangreichen Fachliteratur. Viel作者: compel 時(shí)間: 2025-3-22 07:45 作者: 貪心 時(shí)間: 2025-3-22 08:56 作者: Negotiate 時(shí)間: 2025-3-22 16:33 作者: 掃興 時(shí)間: 2025-3-22 19:42 作者: Debility 時(shí)間: 2025-3-23 01:07
Farhad Kosari,Shaohu Sheng,Thomas R. KleymanAkzentuierung erfahren und müssen unter neuen Bedingungen von den Jugendlichen bearbeitet werden. Das betrifft vor allem die schulische und berufliche "Karriere" mit neuen M?glichkeiten und Risiken. So ist denn auch ein Begriff, der in allen vier Beitr?gen eine bedeutende Rolle spielt, hervorzuheben作者: 惹人反感 時(shí)間: 2025-3-23 03:10 作者: NIB 時(shí)間: 2025-3-23 06:38 作者: NEX 時(shí)間: 2025-3-23 13:29 作者: evasive 時(shí)間: 2025-3-23 17:22 作者: 哎呦 時(shí)間: 2025-3-23 19:21 作者: 雪崩 時(shí)間: 2025-3-24 01:05
Lyubov V. Parfenova,Brad S. Rothbergsst?rken aus dem sichtbaren Wellenl?ngenbereich ausgesetzt sind. In diesem Buch werden daher Untersuchungen des Einflusses von kontinuierlicher und gepulster blauer LED-Strahlung auf transparente Kunststoffe pr?sentiert. Hierzu wird ein neu entwickeltes Prüfverfahren namens .Monitored Liquid Thermos作者: Watemelon 時(shí)間: 2025-3-24 04:34 作者: facilitate 時(shí)間: 2025-3-24 09:31 作者: 催眠 時(shí)間: 2025-3-24 11:07 作者: 發(fā)芽 時(shí)間: 2025-3-24 18:36 作者: 他日關(guān)稅重重 時(shí)間: 2025-3-24 19:35 作者: 者變 時(shí)間: 2025-3-25 01:17 作者: 沒有準(zhǔn)備 時(shí)間: 2025-3-25 06:11
Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using thods to determine the functional subunit stoichiometry of ENaC based on a biophysical approach that was first introduced in 1991 to determine the subunit stoichiometry of a voltage-gated K. channel. The strategy is to analyze channel sensitivity to a specific blocker when various mixtures of block-s作者: 放牧 時(shí)間: 2025-3-25 08:55
Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer AnalysisA key to successful application of green fluorescent protein-based FRET is to reliably separate the FRET signal from various non-FRET fluorescence emissions that coexist in any experimental system. This chapter introduces a FRET quantification method that is based on fluorescence spectroscopic micro作者: Accede 時(shí)間: 2025-3-25 13:00
Probing the Effects of Phosphoinositides on Ion Channels. Membrane phospho-lipids containing phosphoinositides have recently emerged as important regulators of many ion channels, including inward rectifier K . channel, voltage-gated K . and Ca . channels, transient receptor potential channels, and intracellular inositol-1,4,5-trisphos-phate receptor ion 作者: 變態(tài) 時(shí)間: 2025-3-25 18:38
Epithelial Sodium Channel in Planar Lipid Bilayersodium handling, they serve diverse functions in many tissues. Improper functioning of ENaC has been implicated in several diseases, including salt-sensitive hypertension (Liddle’s syndrome), salt-wasting syndrome (pseudohypoaldosteronism type I), pulmonary edema, and cystic fibrosis. We have utilize作者: Melodrama 時(shí)間: 2025-3-25 23:18
A Simple In Vivo Method for Assessing Changes of Membrane-Bound Ion Channel Density in , Oocytesd transporters. In the case of ion channels, activity can be easily measured by conventional two-electrode voltage clamping. However, this method only measures the sum of the activity of all plasma membrane-bound channels. Therefore, this measurement cannot discriminate between effects on channel de作者: Muscularis 時(shí)間: 2025-3-26 01:59
Preparation of Cortical Brain Slices for Electrophysiological Recordingnd whole-cell function in juvenile and adult tissue. Ion channel recordings from small dendritic branches axons in brain slices have demonstrated considerable functional differences in ion channel function within subregions of single cells. These findings have greatly increased understanding of neur作者: 蔑視 時(shí)間: 2025-3-26 04:26 作者: HARP 時(shí)間: 2025-3-26 12:19
Carbon Fiber Amperometry in the Study of Ion Channels and Secretion. signals, the induction of exocytosis—the process in which the contents of secretory vesicles are released by their fusion to the plasma membrane—is one of the most important. For example, in neurons and endocrine cells, it allows intercellular communication and secretion of biomolecules. Exocytosi作者: Narcissist 時(shí)間: 2025-3-26 13:17 作者: 大都市 時(shí)間: 2025-3-26 19:08
KCNQ1 K+ Channel—Mediated Cardiac Channelopathiesciates with different β-subunits of the KCNE protein family. In the human heart, KCNQ1 associates with KCNE1 to generate the .. current characterized by its slow activation and deactivation kinetics. Mutations in either KCNQ1 or KCNE1 are responsible for at least four channelopathies that lead to ca作者: 過多 時(shí)間: 2025-3-27 00:29
Tissue-Specific Transgenic and Knockout Miceciates with different β-subunits of the KCNE protein family. In the human heart, KCNQ1 associates with KCNE1 to generate the .. current characterized by its slow activation and deactivation kinetics. Mutations in either KCNQ1 or KCNE1 are responsible for at least four channelopathies that lead to ca作者: floodgate 時(shí)間: 2025-3-27 03:42 作者: 白楊 時(shí)間: 2025-3-27 08:54 作者: 大炮 時(shí)間: 2025-3-27 12:01
Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression Systemtate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na. channel heterologously expressed in Chinese hamster ovary cells.作者: 一大群 時(shí)間: 2025-3-27 16:18
James D. Stockand,Mark S. ShapiroIncludes supplementary material: 作者: conjunctiva 時(shí)間: 2025-3-27 18:58 作者: hemoglobin 時(shí)間: 2025-3-27 23:21
1064-3745 e their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy作者: 賞錢 時(shí)間: 2025-3-28 04:24
Overexpression of Proteins in Neurons Using Replication-Deficient Virusiological techniques to assess the effects of overexpressing wild-type or mutant proteins in neurons. Here, a general protocol for the production of replicationdeficient Semliki Forest virus constructs directing the overexpression of proteins of interest in cultured mammalian neurons is described.作者: Tracheotomy 時(shí)間: 2025-3-28 09:14
Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using tensitive and blocker-insensitive subunits are coexpressed in a heterologous expression system. Details related to the expression of wild type and mutant ENaCs in . oocytes and the examination of blocker sensitivity by two-electrode voltage clamp, as well as analysis of data are provided.作者: SYN 時(shí)間: 2025-3-28 14:19 作者: 惡心 時(shí)間: 2025-3-28 16:18
Book 2006eterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies.作者: endarterectomy 時(shí)間: 2025-3-28 19:14 作者: AVOID 時(shí)間: 2025-3-29 02:16
A Simple In Vivo Method for Assessing Changes of Membrane-Bound Ion Channel Density in , Oocytesent. This assay is linear over a wide range of channel expression levels and provides a simple cost-effective way of monitoring changes of membrane-bound channel density. Moreover, when the heterologous proteins poorly express at the plasma membrane, this method becomes advantageous to complex biochemical cell fractionation.作者: CURB 時(shí)間: 2025-3-29 06:11
KCNQ1 K+ Channel—Mediated Cardiac Channelopathiesng QT syndrome, characterized by prolonged QT interval, syncopes, and sudden death. Patch clamp and immunofluorescence techniques are instrumental for characterization of the molecular mechanisms responsible for the altered function of KCNQ1 and its partners.作者: Proclaim 時(shí)間: 2025-3-29 10:33
Tissue-Specific Transgenic and Knockout Miceng QT syndrome, characterized by prolonged QT interval, syncopes, and sudden death. Patch clamp and immunofluorescence techniques are instrumental for characterization of the molecular mechanisms responsible for the altered function of KCNQ1 and its partners.作者: 混亂生活 時(shí)間: 2025-3-29 12:48 作者: Hyperplasia 時(shí)間: 2025-3-29 17:34
Probing the Effects of Phosphoinositides on Ion ChannelsK . channel, voltage-gated K . and Ca . channels, transient receptor potential channels, and intracellular inositol-1,4,5-trisphos-phate receptor ion channels. Discussed here are several methods for studying regulation of ion channels by phospholipids.作者: 難聽的聲音 時(shí)間: 2025-3-29 21:22 作者: adduction 時(shí)間: 2025-3-30 01:05
Preparation of Cortical Brain Slices for Electrophysiological Recordingiderable functional differences in ion channel function within subregions of single cells. These findings have greatly increased understanding of neuronal computation. This chapter presents methods for obtaining high-quality brain slices developed to aid visualization of small neuronal structures using differential interference contrast microscopy作者: 擴(kuò)張 時(shí)間: 2025-3-30 05:11
Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System cell cycling and proliferation and is similar to intranuclear microinjection, with both avoiding cDNA delivery through the cytosol. Examples of successful transfection using PDS of rat superior cervical ganglion and trigeminal ganglion neurons are discussed.
