標(biāo)題: Titlebook: Intracellular Pathogens; Methods and Protocol Aneesh Thakur Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lice [打印本頁(yè)] 作者: 分期 時(shí)間: 2025-3-21 18:36
書目名稱Intracellular Pathogens影響因子(影響力)
作者: 傲慢人 時(shí)間: 2025-3-21 23:41
Methods for Genomic Epidemiology of Bacterial Pathogens: Example Salmonella,oved drastically within the past decade. In this chapter, we discuss the prerequisites for good bacterial genomics studies and make note of advantages and disadvantages of this research approach. We discuss methods for outbreak detection and the evolutionary and epidemiological characterization of .作者: Gratulate 時(shí)間: 2025-3-22 02:48 作者: avarice 時(shí)間: 2025-3-22 05:59 作者: 委托 時(shí)間: 2025-3-22 10:36 作者: 放氣 時(shí)間: 2025-3-22 13:38
Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR,r) to the inside of the host cell (intracellular). Nuclear Magnetic Resonance (NMR) is a versatile analytical method, which lends itself for metabolic studies. In this chapter, we describe how .H NMR can be combined with a cellular infection model to study the metabolic crosstalk between a bacterial作者: 割讓 時(shí)間: 2025-3-22 19:34
Quantitative Proteomics of the Intracellular Bacterial Pathogen , Serovar Typhimurium,l as mechanisms of regulation, signaling cascades, interaction partners, and communication patterns across biological systems. For profiling of intracellular pathogens, proteomic profiling can be performed in the absence of a host to singularly define the pathogenic proteome or during an infection-l作者: 輕打 時(shí)間: 2025-3-23 01:09 作者: 籠子 時(shí)間: 2025-3-23 05:15 作者: Critical 時(shí)間: 2025-3-23 07:59
Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research,ese models are particularly valuable for studying emerging respiratory viral and bacterial infections. Here, we describe an optimized protocol to obtain the ALI airway culture models using normal human bronchial epithelial cells (NHBECs). The protocol outlined below enables the generation of differe作者: HUMID 時(shí)間: 2025-3-23 10:05 作者: 王得到 時(shí)間: 2025-3-23 14:57 作者: 財(cái)主 時(shí)間: 2025-3-23 21:33
Intravital Microscopy for Imaging and Live Cell Tracking of Alveolar Macrophages in Real Time,everal pathogens. Coculture assays with pathogens and eukaryotic cells can be analyzed through various techniques including plating for colony-forming units (CFU), confocal microscopy, and flow cytometry. However, findings from in vitro assays require validation in an in vivo model. Several physiolo作者: 領(lǐng)巾 時(shí)間: 2025-3-23 23:22 作者: surmount 時(shí)間: 2025-3-24 02:57
Bacteriophages Isolation and Efficacy Testing,n alternative to antibiotics due to their specificity and ability to efficiently lyse antimicrobial resistant bacterial pathogens. Before using phages, they must be isolated from the environment and tested to ensure purity and lytic ability against various hosts. This protocol walks through the enti作者: configuration 時(shí)間: 2025-3-24 07:48
Production, Isolation, and Detection of Poly IC in Extracellular Vesicles from U937 Cells, body. Extracellular vesicles (EVs) are lipid-bound particles released from cells homeostatically. Recent studies have shown that a commercially available dsRNA, poly inosinic: poly cytidylic acid (poly IC), can be detected within EVs. This finding opens the door for studying EVs as (1) carriers for作者: Defiance 時(shí)間: 2025-3-24 13:51
In Silico Tools for Predicting Novel Epitopes, and adaptation to host immune responses. Historically, antigenicity was studied with experiments that evaluate the immune response against selected fragments of pathogens. Using this approach, the scientific community has gathered abundant information regarding which pathogenic fragments are immuno作者: Kaleidoscope 時(shí)間: 2025-3-24 18:45 作者: 芳香一點(diǎn) 時(shí)間: 2025-3-24 21:29 作者: monologue 時(shí)間: 2025-3-24 23:36 作者: 神圣不可 時(shí)間: 2025-3-25 06:02 作者: 改正 時(shí)間: 2025-3-25 09:54 作者: follicular-unit 時(shí)間: 2025-3-25 15:02 作者: Lime石灰 時(shí)間: 2025-3-25 16:59 作者: 單純 時(shí)間: 2025-3-25 20:31
Nooran Abu Mazen,Jessica Luc,Briallen Lobb,Jeremy Alexander Hirota,Arinjay Banerjee,Andrew C. Doxeyutions.Applies an axiomatic approach, deriving the laws of e.This book is devoted to the fundamentals of classical electrodynamics, one of the most beautiful and productive theories in physics. A general survey on the applicability of physical theories shows that only few theories can be compared to作者: maudtin 時(shí)間: 2025-3-26 01:05 作者: 暴發(fā)戶 時(shí)間: 2025-3-26 05:14
Jennifer Geddes-McAlister,Nicole Hansmeiernowledge to practical developments.Supported throughout with.This engaging text offers an accessible and clear treatment of the fundamentals of electromagnetics and optics, a core part of the standard undergraduate physics curriculum.? Starting with static electric and magnetic fields, the book work作者: quiet-sleep 時(shí)間: 2025-3-26 10:41
Kaushal Baid,Kim R. Chiok,Arinjay Banerjeenowledge to practical developments.Supported throughout with.This engaging text offers an accessible and clear treatment of the fundamentals of electromagnetics and optics, a core part of the standard undergraduate physics curriculum.? Starting with static electric and magnetic fields, the book work作者: APNEA 時(shí)間: 2025-3-26 13:11
Kim R. Chiok,Kaushal Baid,Arinjay Banerjeelectrodynamics of Plasma and Plasma-Like Media (Silin and RuThis book presents a sequential representation of the electrodynamics of conducting media with dispersion. In addition to the general electrodynamic formalism, specific media such as classical nondegenerate plasma, degenerate metal plasma, 作者: GIDDY 時(shí)間: 2025-3-26 17:10
Kim R. Chiok,Nuraina A. Dahlan,Arinjay Banerjee,Neeraj Dharormalism, specific media such as classical nondegenerate plasma, degenerate metal plasma, magnetoactive anisotropic plasma, atomic hydrogen gas, semiconductors, and molecular crystals are considered..The book draws on such classics as .Electrodynamics of plasma and plasma-like media. (Silin and Rukh作者: Esophagitis 時(shí)間: 2025-3-26 22:19 作者: 抑制 時(shí)間: 2025-3-27 03:17
Chiara Toniolo,Daniel Sage,John D. McKinney,Neeraj Dhare- formable bodies are considered to be continua endowed with continuous distributions of mass and charge. The theory of electromagnetic continua is concerned with the determination of deformations, motions, stress, and elec- tromagnetic fields developed in bodies upon the applications of external l作者: optic-nerve 時(shí)間: 2025-3-27 08:11
Ashley Kwak,Ajitha Thanabalasuriarontemporary continuum of physics. The first volume was devoted mainly to the development of the theory and applications to deformable solid media. This volume extends the developments of the first volume to richer and newer grounds. It contains discussions on fluid media, magnetohydrodynamics, eletr作者: Cardioversion 時(shí)間: 2025-3-27 10:14 作者: 極力證明 時(shí)間: 2025-3-27 17:26
Ayesha Lone,Carlos E. Martinez-Soto,Hany Anany the centres of gravity are not ordered in this sense. As a result, the states of matter differ by their mechanical properties; for example, a liquid flows easily. Certain organic materials do not show a single transition from solid to liquid, but rather a cascade of transitions involving new phases作者: 珍奇 時(shí)間: 2025-3-27 20:23 作者: Grating 時(shí)間: 2025-3-27 22:11
Methods for Genomic Epidemiology of Bacterial Pathogens: Example Salmonella, spp. We provide an outline for determining the sequence type and serotype of isolates, building a core genome phylogenetic tree, and detecting antimicrobial resistance genes, virulence factors, and mobile genetic elements. These methods can be used to study other pathogenic bacterial species.作者: 不幸的人 時(shí)間: 2025-3-28 02:34
Quantification of , Growth in Cell-Based Infection Assays by Time-Lapse Fluorescence Microscopy,r quantification of . growth dynamics in host cells. In addition, this approach provides the capability to capture other readouts from the same experimental setup, such as host cell viability, bacterial localization as well as the dynamics of propagation of infection between the host cells.作者: FIN 時(shí)間: 2025-3-28 08:24
Bacteriophages Isolation and Efficacy Testing,re multi-day procedure of enriching?and processing?raw environmental samples (seawater, primary sludge, and soil), testing for lytic activity, selecting and picking potential phage plaques, verifying phage purity, and finally, propagation (liquid and solid) of phages to obtain high-titer crude phage lysates.作者: CORE 時(shí)間: 2025-3-28 10:48 作者: defendant 時(shí)間: 2025-3-28 17:13
Median Tissue Culture Infectious Dose 50 (TCID50) Assay to Determine Infectivity of Cytopathic Virung viruses. Here, we describe the discontinuous median tissue culture infectious dose 50 (TCID.) assay to quantitatively determine the titer of any virus that can produce a visible cytopathic effect in infected cells.作者: 干涉 時(shí)間: 2025-3-28 22:35 作者: Painstaking 時(shí)間: 2025-3-29 00:44 作者: 我說(shuō)不重要 時(shí)間: 2025-3-29 04:41 作者: 咯咯笑 時(shí)間: 2025-3-29 09:31
Phagocytosis,ling to activate the internalization machinery, (iii) phagosome formation, and (iv) phagolysosome maturation. In this chapter, we present a general view of our current knowledge on phagocytosis performed mainly by professional phagocytes through antibody and complement receptors and discuss aspects that remain incompletely understood.作者: Feigned 時(shí)間: 2025-3-29 14:28 作者: N斯巴達(dá)人 時(shí)間: 2025-3-29 18:52
Quantitative Proteomics of the Intracellular Bacterial Pathogen , Serovar Typhimurium,ncluding protein extraction, digestion, and purification, as well as mass spectrometry measurements and bioinformatics analysis. The data generated from our dual perspective profiling approach provides new insight into pathogen and host protein modulation under infection-like conditions.作者: magnate 時(shí)間: 2025-3-29 19:51
Production, Isolation, and Detection of Poly IC in Extracellular Vesicles from U937 Cells,an anti-dsRNA antibody (J2) for detection. Two methods for isolating the EVs and two methods for isolating the RNA from these EVs are described. Together, these methods effectively produce, isolate, and detect long dsRNA from EVs.作者: 機(jī)制 時(shí)間: 2025-3-30 02:56
In Situ Characterization of Human Follicular Helper CD4 T Cells,ity, understanding the cellular and molecular mechanisms regulating Tfh cell development is of great importance. Here, we describe novel approaches for the comprehensive understanding of these cells and possible implications for future studies in vaccine development and the understanding of the pathogenesis of relevant diseases.作者: 諷刺 時(shí)間: 2025-3-30 06:20
Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR, studies. In this chapter, we describe how .H NMR can be combined with a cellular infection model to study the metabolic crosstalk between a bacterial pathogen and its host both in the extracellular and intracellular compartments. Central carbon metabolism is highlighted by using glucose labeled with the stable isotope .C.作者: Pepsin 時(shí)間: 2025-3-30 08:17
Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions,nteractions, accelerating the development of diagnostics, vaccines, and therapeutics. Here, we describe quantitative PCR (qPCR) in studies of virus-host interactions to dissect host responses and viral kinetics and how these relate to one another.作者: outrage 時(shí)間: 2025-3-30 14:02
Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research,in the ALI airway culture models using normal human bronchial epithelial cells (NHBECs). The protocol outlined below enables the generation of differentiated mucociliary airway epithelial cultures by day 28 following exposure to air.作者: 耐寒 時(shí)間: 2025-3-30 18:14 作者: 精美食品 時(shí)間: 2025-3-30 22:31
Pig Acute Phase Proteins as Non-Antibody Systemic Biomarkers of Intracellular Infections,), haptoglobin, serum amyloid A (SAA), and pig major acute phase protein (pig-MAP). The performance of these APPs as biomarkers in a range of experimental infection studies in pigs is described as examples on their use for estimating the severity of infection, vaccine efficacy, herd health character作者: 六個(gè)才偏離 時(shí)間: 2025-3-31 02:17 作者: 形容詞詞尾 時(shí)間: 2025-3-31 05:34 作者: 公豬 時(shí)間: 2025-3-31 10:55 作者: alcoholism 時(shí)間: 2025-3-31 13:45
r regions including limbs, as seen in ALS, or may remain restricted to the bulbar muscles. There are many other diseases that can manifest with some of the classic symptoms of MND such as: weakness, fasciculations, spasticity, cramps, and pathologic hyperreflexia. It is imperative that the clinician作者: laparoscopy 時(shí)間: 2025-3-31 19:18 作者: Isolate 時(shí)間: 2025-3-31 23:40 作者: 敲詐 時(shí)間: 2025-4-1 04:40
Ke-Chuan Wang,Pernille Rose Jensenf undergraduate and graduate studies in physics, astronomy, engineering, applied mathematics and for researchers working in related subjects..We hope that the reader will not only acquire knowledge, but will also grasp the beauty of theoretical physics. A set of about 130 solved and proposed problem作者: 有斑點(diǎn) 時(shí)間: 2025-4-1 08:26 作者: 種子 時(shí)間: 2025-4-1 12:22
Peter M. H. Heegaard,Sofie M. R. Starb?k,Davide Lelli,Kerstin Skovgaardlass of continua. This is done by means of the constitutive theory. For example, an elastic dielectric responds to electromagnetic fields in a totally different way than a magnetic fluid. The present book is intended to present a unified approach to the subject matter, based on the principles of con作者: hardheaded 時(shí)間: 2025-4-1 17:44
