標題: Titlebook: Insoluble Proteins; Methods and Protocol Elena García-Fruitós Book 2015 Springer Science+Business Media New York 2015 Biotechnology.Express [打印本頁] 作者: Radiofrequency 時間: 2025-3-21 18:32
書目名稱Insoluble Proteins影響因子(影響力)
書目名稱Insoluble Proteins影響因子(影響力)學(xué)科排名
書目名稱Insoluble Proteins網(wǎng)絡(luò)公開度
書目名稱Insoluble Proteins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Insoluble Proteins被引頻次
書目名稱Insoluble Proteins被引頻次學(xué)科排名
書目名稱Insoluble Proteins年度引用
書目名稱Insoluble Proteins年度引用學(xué)科排名
書目名稱Insoluble Proteins讀者反饋
書目名稱Insoluble Proteins讀者反饋學(xué)科排名
作者: 放氣 時間: 2025-3-21 22:45
978-1-4939-4675-4Springer Science+Business Media New York 2015作者: Haphazard 時間: 2025-3-22 03:20 作者: coagulation 時間: 2025-3-22 05:15
Transient Expression in HEK 293 Cells: An Alternative to , for the Production of Secreted and IntracTransient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.作者: DIKE 時間: 2025-3-22 11:20 作者: 不適 時間: 2025-3-22 13:18 作者: lactic 時間: 2025-3-22 19:32
General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins,lic bacteria, are gaining interest in this field. However, it is worth mentioning that prokaryotic expression system poses, in many cases, severe restrictions for a successful heterologous protein production. Thus, eukaryotic systems such as mammalian cells, insect cells, yeast, filamentous fungus, 作者: Irritate 時間: 2025-3-23 01:12
Co-translational Stabilization of Insoluble Proteins in Cell-Free Expression Systemsworking concentrations. Examples of screening experiments in order to systematically identify compounds or compound mixtures that stabilize particular proteins of interest are given. The method can primarily be considered for the production of unstable soluble proteins or of membrane proteins contai作者: 反應(yīng) 時間: 2025-3-23 02:55 作者: Affluence 時間: 2025-3-23 07:59
Book 2015ies format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and practical, .Insoluble Proteins: Methods and Protoc作者: 滔滔不絕的人 時間: 2025-3-23 13:26
Ranjana Arya,Jamal S. M. Sabir,Roop S. Bora,Kulvinder S. Sainieceptors Exclusively Activated by Designer Drugs .aims to ensure successful results in the further study of this vital field.978-1-4939-4957-1978-1-4939-2944-3Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: dysphagia 時間: 2025-3-23 15:50
Judit Osz-Papai,Laura Radu,Wassim Abdulrahman,Isabelle Kolb-Cheynel,Nathalie Troffer-Charlier,Cathern six sections according to the main fields of influence, it provides a survey of the state of scientifically-based knowledge and the trends of engineering design research on the influences leading to successful product development.978-1-4471-1270-9978-1-4471-1268-6作者: 填滿 時間: 2025-3-23 19:20 作者: consolidate 時間: 2025-3-23 22:47 作者: 讓步 時間: 2025-3-24 03:13 作者: Biomarker 時間: 2025-3-24 07:05
n six sections according to the main fields of influence, it provides a survey of the state of scientifically-based knowledge and the trends of engineering design research on the influences leading to successful product development.978-1-4471-1270-9978-1-4471-1268-6作者: 無政府主義者 時間: 2025-3-24 10:43
Zhanglin Lin,Qing Zhao,Bihong Zhou,Lei Xing,Wanghui Xuvation. These receptors are modified to couple through cellular machinery resulting in either stimulation or inhibition of neural activity. This allows researchers to acutely and explicitly “turn on” and “turn off” a cell type of choice and assess the resulting behavioral output. Here, I describe ho作者: 考古學(xué) 時間: 2025-3-24 18:01 作者: 憲法沒有 時間: 2025-3-24 22:41 作者: HAIL 時間: 2025-3-25 02:57 作者: 看法等 時間: 2025-3-25 03:45
res, conference presentations and journal papers. In this book I have brought together a selected series of these reports, trying to trace a coherent thread, and to lay out some of the network of arguments and evidence referred to above. My goal has been to understand how designers think, or the nat作者: paltry 時間: 2025-3-25 09:54 作者: Heterodoxy 時間: 2025-3-25 14:11
Anna Villar-Pique,Susanna Navarro,Salvador Venturaelected series of these reports, trying to trace a coherent thread, and to lay out some of the network of arguments and evidence referred to above. My goal has been to understand how designers think, or the nat978-1-84996-573-6978-1-84628-301-7作者: 磨碎 時間: 2025-3-25 16:26 作者: 癡呆 時間: 2025-3-25 20:48
Lei Kai,Erika Orbán,Erik Henrich,Davide Proverbio,Volker D?tsch,Frank Bernhardelected series of these reports, trying to trace a coherent thread, and to lay out some of the network of arguments and evidence referred to above. My goal has been to understand how designers think, or the nat978-1-84996-573-6978-1-84628-301-7作者: 殘廢的火焰 時間: 2025-3-26 03:57
Yasuaki Kawarasaki,Takeshi Kurose,Keisuke Itoelected series of these reports, trying to trace a coherent thread, and to lay out some of the network of arguments and evidence referred to above. My goal has been to understand how designers think, or the nat978-1-84996-573-6978-1-84628-301-7作者: Encumber 時間: 2025-3-26 05:43
1064-3745 ary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and practical, .Insoluble Proteins: Methods and Protoc978-1-4939-4675-4978-1-4939-2205-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Flinch 時間: 2025-3-26 09:57
Neus Ferrer-Miralles,Paolo Saccardo,José Luis Corchero,Zhikun Xu,Elena García-Fruitós作者: aggravate 時間: 2025-3-26 16:13
Book 2015esearchers working in the recombinant protein production field by describing a wide number of protocols and examples. .Insoluble Proteins: Methods and Protocols. includes chapters that describe not only the recombinant protein production in different expression systems but also different purificatio作者: Instinctive 時間: 2025-3-26 19:35 作者: 你正派 時間: 2025-3-27 00:21 作者: PRO 時間: 2025-3-27 02:20
Functional Expression of Plant Membrane Proteins in ,o provide detailed protocols for (1) the expression of plant peripheral or intrinsic membrane proteins and then for (2) their solubilization, from . membranes, for further purification steps and biochemical characterization.作者: initiate 時間: 2025-3-27 07:43 作者: Frequency 時間: 2025-3-27 11:02
High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range ofs to the stationary phase in non-inducing condition, (2) suspend the cells to a small aliquot of inducing medium to form a high cell-density suspension or “a phalanx,” and then (3) give a sufficient aeration to the phalanx. Factors and pitfalls that affect the system’s performance are also described.作者: mettlesome 時間: 2025-3-27 13:56
Insect Cells–Baculovirus System for the Production of Difficult to Express Proteinse the procedure, starting from the cloning of a gene of interest into an expression transfer baculovirus vector, followed by generation of the recombinant virus by homologous recombination, evaluation of protein expression, and scale-up. Handling of insect cell cultures and preparation of bacmid for co-transfection are also detailed.作者: antidote 時間: 2025-3-27 21:11 作者: Presbycusis 時間: 2025-3-27 22:55
Beyond the Cytoplasm of ,: Localizing Recombinant Proteins Where You Want Themcan often be overcome by targeting protein expression to extracytoplasmic compartments (e.g., membrane, periplasm) or to the culture medium. This chapter discusses various strategies for exporting proteins out of the cytoplasm as well as tools for monitoring and optimizing these different export mechanisms.作者: OUTRE 時間: 2025-3-28 04:52
Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purificationed intein self-cleavage reaction releases the target protein into solution. These cleavable self-aggregating tags (cSAT, intein-18A/ELK16) provide a quick and efficient route for the production of proteins with modest purity (around 90 % in the case of intein-ELK16). Two application examples are included in the chapter.作者: 漸強 時間: 2025-3-28 09:36
General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins,luble and functional recombinant proteins is among the main goals in the biotechnological field. In this context, it is important to point out that under stress conditions, protein folding machinery is saturated and this promotes protein misfolding and, consequently, protein aggregation. Thus, the s作者: 裹住 時間: 2025-3-28 12:47
Overcoming the Solubility Problem in ,: Available Approaches for Recombinant Protein Productionneous product are still unsolved. Although several strategies were developed to overcome these obstacles, at present there is no magic bullet that can be applied for all cases. Indeed, several key expression parameters need to be evaluated for each protein. Among the different hosts for protein expr作者: 珊瑚 時間: 2025-3-28 14:43 作者: 極小 時間: 2025-3-28 20:01 作者: notice 時間: 2025-3-29 00:17 作者: Mnemonics 時間: 2025-3-29 03:06 作者: 讓空氣進入 時間: 2025-3-29 09:52
Co-translational Stabilization of Insoluble Proteins in Cell-Free Expression Systemsccessibility of cell-free reactions allows addressing such critical steps by the addition of protein stabilizers such as chemical chaperones or detergents directly into the expression reactions. This approach could therefore reduce or even prevent initial protein precipitation already in the transla作者: 熟練 時間: 2025-3-29 13:56
Functional Expression of Plant Membrane Proteins in ,c bacterium, has been traditionally used in food fermentations and is nowadays widely used in biotechnology for large-scale production of heterologous proteins. This system has been successfully used for the production of prokaryotic and eukaryotic membrane proteins. The purpose of this chapter is t作者: 健談的人 時間: 2025-3-29 16:26 作者: Initiative 時間: 2025-3-29 23:17 作者: 串通 時間: 2025-3-30 00:46
Recombinant Glycoprotein Production in Human Cell Linesified by a large number of posttranslational modifications. These modifications can occur during or after the synthesis process, and glycosylation appears as the most common posttranslational modification. It is estimated that 50 % of human proteins have some kind of glycosylation, which has a key r作者: regale 時間: 2025-3-30 04:54
Ranjana Arya,Jamal S. M. Sabir,Roop S. Bora,Kulvinder S. Sainiation advice from the expertsThis volume provides current methods to analyze the properties of designer receptors exclusively activated by designer drugs (DREADDs) in vitro and to measure the biological responses of DREADD activation in different neuronal populations in vivo. Chapters focus on the u作者: 收集 時間: 2025-3-30 09:49
Zhanglin Lin,Qing Zhao,Bihong Zhou,Lei Xing,Wanghui Xuh human history by examining how it has shaped our genes, cultures, and landscapes. Yet, despite the shared familiarity of eating, the neural circuits underlying the motivational processes underlying shifts in appetite have remained elusive due to a lack of methods sensitive enough to probe defined 作者: FANG 時間: 2025-3-30 15:58
Jason T. Boock,Dujduan Waraho-Zhmayev,Dario Mizrachi,Matthew P. DeLisathat research in design seemed to have no clear goal of what it was trying to achieve. A key insight for me was to realise that if we wanted to develop a robust, independent discipline of design (rather than let design be subsumed within paradigms of science or the arts), then we had to be much more作者: 合唱團 時間: 2025-3-30 20:01
Anna Villar-Pique,Susanna Navarro,Salvador Ventura of design research.Provides a unique insight into a field oThis book traces the development of a personal research programme over a period of many years. The starting point for the programme was a realisation that research in design seemed to have no clear goal of what it was trying to achieve. A k作者: Trochlea 時間: 2025-3-31 00:20
