作者: 思考 時(shí)間: 2025-3-21 21:38 作者: single 時(shí)間: 2025-3-22 02:12 作者: 驚奇 時(shí)間: 2025-3-22 07:49 作者: rheumatism 時(shí)間: 2025-3-22 12:33 作者: 手工藝品 時(shí)間: 2025-3-22 16:43 作者: 外科醫(yī)生 時(shí)間: 2025-3-22 21:01
Bernard Thisse,Christine Thisseofferedin Information Studies, Information Systems, or Information Technology. It also serves as an excellent practical guide for professionals in information management and provides important support material for courses in Computer Science and in Business.978-1-4899-9380-9978-1-4614-0992-2作者: –LOUS 時(shí)間: 2025-3-23 00:00 作者: 把手 時(shí)間: 2025-3-23 03:58 作者: Transfusion 時(shí)間: 2025-3-23 09:11 作者: 跳脫衣舞的人 時(shí)間: 2025-3-23 10:24 作者: 來這真柔軟 時(shí)間: 2025-3-23 15:00
Pernille A. Usher,Elisabeth D. Galsgaard,Kimberly Kruse,Jishu Wang,Berit O. Krogh,Jette Mandelbaum,Kofferedin Information Studies, Information Systems, or Information Technology. It also serves as an excellent practical guide for professionals in information management and provides important support material for courses in Computer Science and in Business.978-1-4899-9380-9978-1-4614-0992-2作者: 休戰(zhàn) 時(shí)間: 2025-3-23 21:25 作者: 維持 時(shí)間: 2025-3-24 00:13 作者: absolve 時(shí)間: 2025-3-24 03:56 作者: Odyssey 時(shí)間: 2025-3-24 07:31
,Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris? RNA Fluorescencbe sets with spectrally distinct fluorophores, multiplex analysis of specific RNAs, or RNA variants, can be achieved. We focus on the detection of a cytoplasmic mRNA and a nuclear long noncoding RNA to illustrate the benefits of this method for cell-specific detection and subcellular localization. P作者: FOLLY 時(shí)間: 2025-3-24 12:29
Quantitative Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope,uantitative manual scoring and software-assisted quantitative analysis. Here, we present detailed protocols of RNAscope for FFPE tissue sections. The step-by-step protocols describe tissue preparation, pretreatment, probe hybridization, signal amplification, visualization, and analysis. We also high作者: falsehood 時(shí)間: 2025-3-24 18:00
Hongwei Wang,Nan Su,Li-Chong Wang,Xingyong Wu,Son Bui,Allissa Nielsen,Hong-Thuy Vo,Yuling Luo,Xiao-J作者: 空氣傳播 時(shí)間: 2025-3-24 22:54 作者: 稱贊 時(shí)間: 2025-3-25 01:04 作者: 頭腦冷靜 時(shí)間: 2025-3-25 06:50 作者: CURB 時(shí)間: 2025-3-25 10:49
Fully Automated Fluorescence-Based Four-Color Multiplex Assay for Co-detection of MicroRNA and Protfor co-detection of a microRNA (e.g., let-7a, miR-10b, miR-21, miR-34a, miR-126, miR-145, miR-155, miR-205, miR-210), reference RNA (e.g., U6 snRNA, 18S rRNA), and protein markers (e.g., CD11b, CD20, CD45, collagen I, cytokeratin 7, cytokeratin 19, smooth muscle actin, tubulin, vimentin) in FDA-approved Leica Bond-MAX staining station.作者: foliage 時(shí)間: 2025-3-25 13:40 作者: rectum 時(shí)間: 2025-3-25 19:49 作者: 尾巴 時(shí)間: 2025-3-25 21:56 作者: 破布 時(shí)間: 2025-3-26 03:08
Whole-Mount In Situ Hybridization Using DIG-Labeled Probes in Planarian,ization (WFISH) protocol optimized over the last years by several labs from the rapidly growing planaria field and give an overview of recently introduced modifications which can be critical in the study of low abundant transcripts.作者: burnish 時(shí)間: 2025-3-26 08:05 作者: 繞著哥哥問 時(shí)間: 2025-3-26 10:18
Fluorescence In Situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections,ent in situ hybridization method, combining locked nucleic acid technology and tyramide signal amplification system, has been developed and presented for detection of microRNAs in frozen section at a cellular resolution and with high sensitivity.作者: prosthesis 時(shí)間: 2025-3-26 14:17 作者: 耐寒 時(shí)間: 2025-3-26 19:11
Book 2014Latest editionps on troubleshooting and avoiding known pitfalls..Authoritative and practical, .In Situ Hybridization Protocols, Fourth Edition .seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions..作者: 水槽 時(shí)間: 2025-3-26 21:33 作者: Hearten 時(shí)間: 2025-3-27 04:00
Dual-Color Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope,e 2-Plex assays for FFPE tissue sections. The protocols describe the tissue preparation, pretreatment, probe hybridization, signal amplification, visualization, and analysis, as well as emphasize the critical steps for ensuring successful staining.作者: Subdue 時(shí)間: 2025-3-27 07:10 作者: SOBER 時(shí)間: 2025-3-27 09:45 作者: micturition 時(shí)間: 2025-3-27 16:58 作者: 圍裙 時(shí)間: 2025-3-27 20:23
,Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epint protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of low-expressing transcripts in formalin-fixed paraffin-embedded murine tissues. Examples of positive (high-expressing) and negative (knock out) control samples are provided to describe a case study.作者: 命令變成大炮 時(shí)間: 2025-3-28 00:39 作者: EXCEL 時(shí)間: 2025-3-28 06:02 作者: jeopardize 時(shí)間: 2025-3-28 06:36
Boye Schnack NielsenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: laparoscopy 時(shí)間: 2025-3-28 14:08 作者: 承認(rèn) 時(shí)間: 2025-3-28 14:36 作者: 取之不竭 時(shí)間: 2025-3-28 21:24
978-1-4939-5526-8Springer Science+Business Media New York 2014作者: 系列 時(shí)間: 2025-3-28 23:25 作者: 高深莫測(cè) 時(shí)間: 2025-3-29 03:33
Fixation/Permeabilization Procedure for mRNA In Situ Hybridization of Zebrafish Whole-Mount Oocyteshe simultaneous fixation/permeabilization of samples using formaldehyde as fixative and short C-chain aliphatic carboxylic acids, particularly glacial acetic acid, as permeabilizers. As compared with in situ hybridization performed with routine methods, our procedure is simpler and provides better s作者: ineluctable 時(shí)間: 2025-3-29 09:28 作者: Onerous 時(shí)間: 2025-3-29 11:32 作者: ROOF 時(shí)間: 2025-3-29 19:03
Whole-Mount In Situ Hybridization Using DIG-Labeled Probes in Planarian,ybridization (WISH) and fluorescent in situ hybridization (FISH) are key and most commonly used techniques to determine and visualize gene expression patterns in planaria. Here, we present the established version of whole-mount in situ hybridization (WISH) and whole-mount fluorescence in situ hybrid作者: 沒花的是打擾 時(shí)間: 2025-3-29 22:27 作者: 外露 時(shí)間: 2025-3-30 02:06
LNA-Based In Situ Hybridization Detection of mRNAs in Embryos,perimental manipulations. ISH using short DNA probes containing locked nucleic acid nucleotides (LNAs) holds the additional advantage of allowing the detection of specific RNA splice variants or of closely related family members that differ in only short regions, creating new diagnostic and detectio作者: 影響深遠(yuǎn) 時(shí)間: 2025-3-30 04:38
,Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNA? Probe Technology,sections has been facilitated by locked nucleic acid (LNA)-based probe technology and can be performed within a single working day. In the current method paper, we present a similar simple 1-day ISH method developed for cryostat sections obtained from clinical cryo-embedded tissue samples. The prese作者: 人類 時(shí)間: 2025-3-30 11:53 作者: Parabola 時(shí)間: 2025-3-30 15:11
Sensitive and Specific In Situ Hybridization for Early Drug Discovery,ted genes. To further characterize the expression of selected genes, in situ hybridization (ISH) using RNA probes (riboprobes) is a powerful tool to localize mRNA expression at the cellular level in normal and diseased tissues, especially for novel drug targets, where research tools like specific an作者: Orthodontics 時(shí)間: 2025-3-30 16:41 作者: 生氣的邊緣 時(shí)間: 2025-3-30 20:46
Dual-Color Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope,iple targets simultaneously is especially valuable, since it allows dissecting gene expression of distinct cell types with precise cellular and subcellular resolution within morphological context. Recently, we have reported using a novel dual-color ultrasensitive bright-field RNA in situ hybridizati作者: NOTCH 時(shí)間: 2025-3-31 02:35 作者: Negotiate 時(shí)間: 2025-3-31 05:19
Multiplexed miRNA Fluorescence In Situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues, other reference RNAs, in archival tissues. Some miRNAs are excellent disease biomarkers due to their abundance and cell-type specificity. However, these short RNA molecules are difficult to visualize due to loss by diffusion, probe mishybridization, and signal detection and signal amplification iss作者: 不自然 時(shí)間: 2025-3-31 11:04 作者: Jogging 時(shí)間: 2025-3-31 16:53 作者: 可憎 時(shí)間: 2025-3-31 19:12 作者: Palatial 時(shí)間: 2025-4-1 01:14 作者: 溫和女孩 時(shí)間: 2025-4-1 03:26
