標(biāo)題: Titlebook: In Situ Hybridization Protocols; K. H. Andy Choo Book Oct 19941st edition Humana Press 1995 [打印本頁(yè)] 作者: Remodeling 時(shí)間: 2025-3-21 17:33
書(shū)目名稱(chēng)In Situ Hybridization Protocols影響因子(影響力)
作者: CREST 時(shí)間: 2025-3-21 22:30
Mapping Human YAC Clones by Fluorescence In Situ Hybridization Using Alu-PCR from Single Yeast ColonThe yeast artificial chromosome (YAC) cloning system is capable of cloning large segments of DNA (50–2000 kb) from complex genomes (.). YAC genomic libraries have been constructed using DNA from human as well as other species and have been proven to be powerful tools for the analysis of the human genome.作者: Uncultured 時(shí)間: 2025-3-22 02:20 作者: 監(jiān)禁 時(shí)間: 2025-3-22 04:35 作者: 有節(jié)制 時(shí)間: 2025-3-22 09:27 作者: amplitude 時(shí)間: 2025-3-22 14:32 作者: 潛移默化 時(shí)間: 2025-3-22 17:23 作者: 古董 時(shí)間: 2025-3-23 00:06 作者: 思想 時(shí)間: 2025-3-23 03:47
Reverse Chromosome Paintinglabeled, the composition of the aberrant chromosome is revealed by the distribution of the fluorescence signals obtained with the different probes. The combination of different labeled probes and multicolor techniques simplifies the evaluation.作者: Narcissist 時(shí)間: 2025-3-23 09:07
In Situ Hybridization Using Synthetic Oligomers as Probes for Centromere and Telomere Repeats the genome have the same terminal repeat. During the last 5 years, . hybridization of the telomere sequence has been used for a variety of studies (.– .). The integrity of the telomere as a structural and functional necessity for accurate chromosomal distribution during cell division has been supported by these studies.作者: Fermentation 時(shí)間: 2025-3-23 11:50
Gene Mapping Using 3H-Labeled Heterologous Probesng may be accomplished without the necessity of cloning each gene. Thus it is possible to construct physical genetic maps for any mammalian species without the time-consuming process of cloning the homologous sequences.作者: 障礙 時(shí)間: 2025-3-23 16:55 作者: overrule 時(shí)間: 2025-3-23 21:20
Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase s. Blocks of consecutive monomers comprise a chromosome-specific higher-order repeat of up to several kilobases (kb) in size. For most human chromosomes, specific multimeric higher-order repeat units have been described (for review, . ref. .).作者: 輕彈 時(shí)間: 2025-3-23 23:00
Primed In Situ (PRINS) Labeling of DNAetection methods have become available, resulting in a much more widespread use of . hybridization. These methods have become of great value, e.g., as a supplement to traditional cytogenetic analysis in the identification and quantification of numerical and/or structural aberrations in both clinical genetics and cancer diagnosis.作者: 許可 時(shí)間: 2025-3-24 04:19 作者: 指令 時(shí)間: 2025-3-24 08:09
Free Chromatin Mapping by FISHn provide all the information required for detailed analysis of genomes with greater than several billion base pairs (bp) and each method has its limitation in range of resolution. A combination of different techniques is therefore necessary for any large-scale gene mapping endeavors.作者: 嬉耍 時(shí)間: 2025-3-24 12:31 作者: 粘連 時(shí)間: 2025-3-24 14:50
Book Oct 19941st editionThere is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more sophisticated biological questions and clinical problems. For the n- ice, there should thus be a sense 作者: lavish 時(shí)間: 2025-3-24 22:01 作者: 考博 時(shí)間: 2025-3-25 02:05
putes the idea that spontaneous spoken discourse is flawed, .In contrast to traditional approaches of mainstream psycholinguists, the authors of Communicating with One Another approach spontaneous spoken discourse as a dynamic process, rich with structures, patterns, and rules other than conventiona作者: Range-Of-Motion 時(shí)間: 2025-3-25 04:48
Nicoletta Archidiacono,Rachele Antonacci,Antonino Forabosco,Mariano Rocchiputes the idea that spontaneous spoken discourse is flawed, .In contrast to traditional approaches of mainstream psycholinguists, the authors of Communicating with One Another approach spontaneous spoken discourse as a dynamic process, rich with structures, patterns, and rules other than conventiona作者: 整潔漂亮 時(shí)間: 2025-3-25 09:11
William G. Kearns,Peter L. Pearsonrse as a dynamic process, rich with structures, patterns, and rules other than conventional grammar and syntax. Daniel C. O’Connell and Sabine Kowal thoroughly critique mainstream psycholinguistics, proposing instead a shift in theoretical focus from experimentation to field observation, from monolo作者: 長(zhǎng)矛 時(shí)間: 2025-3-25 13:30 作者: 對(duì)待 時(shí)間: 2025-3-25 16:57 作者: 籠子 時(shí)間: 2025-3-25 22:11
Christoph Lengauer,Ian Dunham,Terry Featherstone,Thomas Cremerrse as a dynamic process, rich with structures, patterns, and rules other than conventional grammar and syntax. Daniel C. O’Connell and Sabine Kowal thoroughly critique mainstream psycholinguistics, proposing instead a shift in theoretical focus from experimentation to field observation, from monolo作者: SCORE 時(shí)間: 2025-3-26 02:10 作者: NOCT 時(shí)間: 2025-3-26 04:37
Antonio Baldini,Elizabeth A. Lindsayputes the idea that spontaneous spoken discourse is flawed, .In contrast to traditional approaches of mainstream psycholinguists, the authors of Communicating with One Another approach spontaneous spoken discourse as a dynamic process, rich with structures, patterns, and rules other than conventiona作者: 擔(dān)憂(yōu) 時(shí)間: 2025-3-26 10:52 作者: Cumbersome 時(shí)間: 2025-3-26 15:09 作者: 盡責(zé) 時(shí)間: 2025-3-26 20:41 作者: Heterodoxy 時(shí)間: 2025-3-26 21:26 作者: NOVA 時(shí)間: 2025-3-27 02:11 作者: 緩解 時(shí)間: 2025-3-27 07:50 作者: 正常 時(shí)間: 2025-3-27 09:54 作者: pacifist 時(shí)間: 2025-3-27 15:38 作者: 整體 時(shí)間: 2025-3-27 20:58 作者: amyloid 時(shí)間: 2025-3-27 23:08
Preparation of Human Chromosomal Painting Probes From Somatic Cell Hybridshis technique allows the painting of specific chromosomes in metaphase spreads and in interphase nuclei (.–.). The usefulness of FSCL is particularly evident, for instance, in identifying the chromosomal origin of . unbalanced translocations and marker chromosomes, or, more generally, in characteriz作者: Cryptic 時(shí)間: 2025-3-28 05:46
Fluorescent In Situ Hybridization Using Chromosome- Specific DNA Librariesroutine and high resolution cytogenetic studies plays an important role in the diagnosis and treatment of disease. However, this analysis is relatively gross and only permits the visual diagnosis of aberrations of single chromosome bands on the order of seven million or so base pairs. FISH using chr作者: 受人支配 時(shí)間: 2025-3-28 07:07
Reverse Chromosome Paintingy fluorescence in . hybridization (FISH) of such mixtures established from a number of different sources (.–.). This technique is a valuable supplemental method in the detailed analysis of specific chromosome aberrations, complex chromosome rearrangements, and chromosomes of poor quality, which have作者: 游行 時(shí)間: 2025-3-28 10:46
FISH Detection on DAPI—Banded Chromosomesequences on human metaphase chromosomes (.). It is often difficult, however, to precisely position the hybridization signals with respect to the conventional chromosome bands. Various approaches have been introduced, including G banding before or after FISH detection (., .), cohybridization with Alu作者: Fortify 時(shí)間: 2025-3-28 17:02
Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase basic unit is a monomer repeat of approx 170 basepair (bp) that contains both sequences conserved among the different chromosomes and variable regions. Blocks of consecutive monomers comprise a chromosome-specific higher-order repeat of up to several kilobases (kb) in size. For most human chromosom作者: dry-eye 時(shí)間: 2025-3-28 19:42 作者: 積習(xí)難改 時(shí)間: 2025-3-29 00:04 作者: Bmd955 時(shí)間: 2025-3-29 07:06
Primed In Situ (PRINS) Labeling of DNAdetection was possible, which to some extent made these techniques unsuited for routine purposes. Within the last decade, a number of nonradioactive detection methods have become available, resulting in a much more widespread use of . hybridization. These methods have become of great value, e.g., as作者: gentle 時(shí)間: 2025-3-29 08:28
Free Chromatin Mapping by FISHctrophoresis and blot-hybridization analysis, DNA sequencing, construction of somatic cell hybrid lines, . hybridization, as well as genetic (linkage) analysis. Because of their wide application, numerous improvements have been made in each of these areas in recent years, for example, the use of pul作者: 戲服 時(shí)間: 2025-3-29 12:39 作者: 固定某物 時(shí)間: 2025-3-29 16:12
In Situ Hybridization of Meiotic Prophase Chromosomesis in part, a result of traditional fixation procedures that use alcohol, acetic acid, and/or relatively strong ionic conditions, all of which can cause chromosome condensation. The limit of resolution may be improved by using free chromatin or DNA-halo preparations as described in . and . of this v作者: 無(wú)彈性 時(shí)間: 2025-3-29 21:24
Strand-Specific Fluorescence In Situ Hybridization for Determining Orientation and Direction of DNA tation and strand direction of DNA sequences (.). Standard fluorescent . hybridization methods rely on nucleotide base pairing between a labeled probe and the complementary chromosomal target sequence. Both probe and target DNAs must be functionally single-stranded at the start of the procedure. Sin作者: 懲罰 時(shí)間: 2025-3-30 01:30 作者: 不可救藥 時(shí)間: 2025-3-30 06:15
Gene Mapping Using 3H-Labeled Heterologous Probese cloned probe, labeled by a radioactive or fluorescent tag, hybridizes specifically to the single-stranded sequence(s) in the genome, and the location of the gene or sequence of interest on the chromosome is visualized by autoradiography or fluorography. Here we will discuss only radioactive . hybr作者: Antarctic 時(shí)間: 2025-3-30 11:16
Dual- label fluorescence in situ hybridization detection of individual chromosomes in sperm using ejtion (FISH) techniques, has added much to the power of the technique for the detection of specific chromosomes in sperm. The technique has many uses, for example, screening for aneuploidy either at the base rate in untreated individuals or in those who have been exposed to cytotoxic chemicals and/or作者: 斷言 時(shí)間: 2025-3-30 14:48
In situ hybridization to drosophila polytene chromosomestic analysis of .. The power of . hybridization is due largely to the scale of polytene chromosomes and consequently the high degree of resolution they offer the researcher. The use of radiolabeled probes has now been largely superseded by nonradioactive signal detection systems, generally using bio作者: 小溪 時(shí)間: 2025-3-30 19:35
In situ hybridization to polytene chromosomes of drosophila melanogaster and other dipteran speciesradioactively labeled probes by Pardue, who has recently published an extensive account of . polytene chromosomes and hybridization to these chromosomes (.). The original method was later adapted to allow the use of the more sensitive and safer nonradioactive labeling methods (.). The method has bee作者: 展覽 時(shí)間: 2025-3-30 20:51
Book Oct 19941st editionnique in order to assist such workers in rapidly advancing toward this goal. With this aim in mind, each of the protocol chapters opens with a detailed Materials section, and an easy-to-follow Methods section. Many readers will find the section headed Notes particularly useful. This s- tion contains作者: neurologist 時(shí)間: 2025-3-31 04:31
1064-3745 ation technique in order to assist such workers in rapidly advancing toward this goal. With this aim in mind, each of the protocol chapters opens with a detailed Materials section, and an easy-to-follow Methods section. Many readers will find the section headed Notes particularly useful. This s- tion contains作者: HERTZ 時(shí)間: 2025-3-31 07:21 作者: 漂亮才會(huì)豪華 時(shí)間: 2025-3-31 13:04 作者: acrobat 時(shí)間: 2025-3-31 16:28 作者: 現(xiàn)任者 時(shí)間: 2025-3-31 19:43 作者: collateral 時(shí)間: 2025-3-31 22:53
Antonio Baldini,Elizabeth A. Lindsaycation, including: (1) The speaker’s use of prosody. (2) The functions of interjections. (3) What fillers do for a living. (4) Turn-taking: Smooth and otherwise. (5) Laughter, applause, and booing: from individ978-1-4419-2660-9978-0-387-77632-3作者: oblique 時(shí)間: 2025-4-1 05:07
Christoph Lengauer,Michael R. Speicher,Thomas Cremercation, including: (1) The speaker’s use of prosody. (2) The functions of interjections. (3) What fillers do for a living. (4) Turn-taking: Smooth and otherwise. (5) Laughter, applause, and booing: from individ978-1-4419-2660-9978-0-387-77632-3作者: cancellous-bone 時(shí)間: 2025-4-1 06:56 作者: GULLY 時(shí)間: 2025-4-1 11:35
