標題: Titlebook: In Situ Detection of DNA Damage; Methods and Protocol Vladimir V. Didenko Book 20021st edition Springer Science+Business Media New York 200 [打印本頁] 作者: Sediment 時間: 2025-3-21 19:00
書目名稱In Situ Detection of DNA Damage影響因子(影響力)
書目名稱In Situ Detection of DNA Damage影響因子(影響力)學科排名
書目名稱In Situ Detection of DNA Damage網(wǎng)絡(luò)公開度
書目名稱In Situ Detection of DNA Damage網(wǎng)絡(luò)公開度學科排名
書目名稱In Situ Detection of DNA Damage被引頻次
書目名稱In Situ Detection of DNA Damage被引頻次學科排名
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書目名稱In Situ Detection of DNA Damage年度引用學科排名
書目名稱In Situ Detection of DNA Damage讀者反饋
書目名稱In Situ Detection of DNA Damage讀者反饋學科排名
作者: TRACE 時間: 2025-3-21 21:06
Vladimir V. DidenkoIncludes supplementary material: 作者: prediabetes 時間: 2025-3-22 04:06
Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/462982.jpg作者: NEG 時間: 2025-3-22 07:47
978-1-61737-272-8Springer Science+Business Media New York 2002作者: Hippocampus 時間: 2025-3-22 12:05
In Situ Detection of DNA Damage978-1-59259-179-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: humectant 時間: 2025-3-22 16:13
1064-3745 ing apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the ex作者: 窗簾等 時間: 2025-3-22 20:29 作者: 不容置疑 時間: 2025-3-23 01:13
DNA Damage Detection Using DNA Polymerase I or its Klenow Fragmenty fields of cellular biology and medicine. This interest has greatly stimulated research on technical improvements of the assays and has led to the availability of a variety of commercial kits for that purpose.作者: MOTIF 時間: 2025-3-23 03:33 作者: 放氣 時間: 2025-3-23 07:29 作者: 裙帶關(guān)系 時間: 2025-3-23 09:51
DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections 3′ ends of DNA molecules, thus providing a sensitive assay for detecting apoptotic cells in tissues. Since the publication of this method, this work has been cited in over 4,300 publications, attesting to the usefulness of the assay.作者: Vulnerable 時間: 2025-3-23 17:53
The Comet Assayage is present in cells, but what form it takes. Although it is essentially a method for measuring DNA breaks, the introduction of lesion-specific endonucleases allows detection of, for example, UV-induced pyrimidine dimers, oxidized bases and alkylation damage.作者: 不規(guī)則 時間: 2025-3-23 18:12
Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and o known that normal epithelial cell proliferation and death in mammalian prostatic glands depend upon an appropriate level of circulating androgen in blood (.,.). Therefore, the prostatic epithelial cells routinely undergo apoptosis by androgen withdrawal following castration in experimental male animals (.,.,.).作者: 扔掉掐死你 時間: 2025-3-23 23:11
Nick Translation at the Electron Microscopic Levelhe new DNA strand behind it using 5′→3′ polymerase activity (.). When labeled deoxyribonucleotides are used as substrates, the original unlabeled nucleotides in the DNA template are replaced by the labeled ones.作者: 性行為放縱者 時間: 2025-3-24 04:52
The Comet Assaysing a fluorescence microscope. Individual images can then be digitized and analyzed for informative properties such as the distance the DNA has migrated and the percent of DNA that has migrated. These features give an indication of the number of strand breaks present in the cell.作者: 易受騙 時間: 2025-3-24 10:10
Book 20021st edition, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently,作者: 小蟲 時間: 2025-3-24 14:13 作者: EVEN 時間: 2025-3-24 15:28
zu geben. Im Gegensatz zu früheren Darstellungen der photographischen Optik, die mehr oder weniger vollst?ndige Konstruktionsdaten und Korrektionskurven bekannter Objektive gaben, soll hier mehr die Wesenheit der . herausgearbeitet werden. An Hand ausgew?hlter Beispiele (siehe Anhang) für den jeweil作者: Promotion 時間: 2025-3-24 23:02 作者: TRUST 時間: 2025-3-25 02:32 作者: Debark 時間: 2025-3-25 06:44 作者: 催眠藥 時間: 2025-3-25 10:14
Yoshinori Otsuki,Yuko Itobesonderer Bedeutung sind: Der Nuklearkrieg spielt als bislang – mit Ausnahme der beiden ersten und einzigen Atomwaffeneins?tze 1945 – nur theoretische, aber gleichwohl praktisch wie konzeptionell intensiv vorbereitete ?ultimative“ Form des Krieges dahingehend eine besondere Rolle, dass er wohl . de作者: 歌曲 時間: 2025-3-25 13:15 作者: Ruptured-Disk 時間: 2025-3-25 15:53 作者: 擋泥板 時間: 2025-3-25 23:00 作者: 不可侵犯 時間: 2025-3-26 01:19 作者: 小卷發(fā) 時間: 2025-3-26 06:28
Katherine A. Woodtzten Ausführungen verdeutlichen, in seinen Grundfesten tief in der klassischen Paradigmatik managementtheoretischer Konzeptionen verhaftet bleibt. Sei es das Grundverst?ndnis der Unternehmensrolle im gesellschaftlichen Kontext, sei es der prinzipielle Organisationszugang, das Verst?ndnis gegenüber 作者: legislate 時間: 2025-3-26 12:17 作者: FILTH 時間: 2025-3-26 15:33
tzten Ausführungen verdeutlichen, in seinen Grundfesten tief in der klassischen Paradigmatik managementtheoretischer Konzeptionen verhaftet bleibt. Sei es das Grundverst?ndnis der Unternehmensrolle im gesellschaftlichen Kontext, sei es der prinzipielle Organisationszugang, das Verst?ndnis gegenüber 作者: 拘留 時間: 2025-3-26 20:24 作者: AMITY 時間: 2025-3-26 21:48
Vladimir V. Didenkoreproduktivit?t und Mutabilit?t, bei Reinhard W. KAPLAN in etwas abge?nderter Form ?Stoffwechsel, Vermehrung und Entwicklung“ und bei Konrad LORENZ ?Ganzheitlichkeit, Finalit?t und historisches Gewordensein“. Versuchen wir demgegenüber nicht das Lebewesen, sondern seinen lebendigen Zustand in seinem作者: 暫時休息 時間: 2025-3-27 04:10 作者: Arroyo 時間: 2025-3-27 08:24 作者: DEVIL 時間: 2025-3-27 09:51 作者: 輕快來事 時間: 2025-3-27 14:00 作者: Obverse 時間: 2025-3-27 19:38
Andrei L. Kindzelskii,Howard R. Petty mittleren Unternehmen (KMU) am 2. Oktober 1998 in Raunheim bei Frankfurt.. Der damalige Werksleiter eines Herstellers für Bodenbearbeitungsger?te aus Nordrhein-Westfalen rüttelte damit sein Auditorium ordentlich wach. über 50 deutsche KMU-Vertreter hatten sich zu einem gemeinsamen Erfahrungsaustaus作者: aristocracy 時間: 2025-3-27 21:55 作者: 背叛者 時間: 2025-3-28 04:20 作者: 蔑視 時間: 2025-3-28 08:18 作者: 委派 時間: 2025-3-28 12:57 作者: SUE 時間: 2025-3-28 14:50
Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and lly defined by its unique ultrastructural features, which were detected by electron microscopy (.,.). They are cytoplasmic shrinkage, nuclear chromatin condensation along nuclear margin, cell fragmentation into apoptotic bodies, and phagocytosis by adjacent epithelial cells or macrophages. It is als作者: 撤退 時間: 2025-3-28 20:24
Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometryristic features of apoptotic cells (.,.). A widely used methodology to detect apoptotic cells, thus, relies on labeling DNA strand breaks in situ, within the nuclear chromatin, either with fluorochromes (.–.) or absorption dyes (.–.). The overview of the techniques (TUNEL techniques), which were dev作者: obstinate 時間: 2025-3-28 23:09
DNA Damage Detection Using DNA Polymerase I or its Klenow Fragmentf cell death, during the past few years polymerase-based assays for detection of DNA breaks in cellular nuclei have found worldwide application in many fields of cellular biology and medicine. This interest has greatly stimulated research on technical improvements of the assays and has led to the av作者: patriot 時間: 2025-3-29 06:23
Labeling DNA Breaks , by Klenow Enzymeerg polymerase of .. Klenow is now usually obtained as a recombinant protein expressed from a truncated . gene (.). To polymerize the addition of nucleotides to the 3′-OH end of DNA, Klenow requires a single stranded DNA template and a DNA or RNA primer with a 3′?hydroxyl terminus. Klenow lacks the 作者: avarice 時間: 2025-3-29 10:39
Nick Translation at the Electron Microscopic Levelies on combined 5′→3′ polymerase and 5′→3′ exonuclease activities of . DNA polymerase I. If a nick or single strand break with a 3′ hydroxyl terminus is present in a duplex DNA molecule, the enzyme will translocate it, removing nucleotides ahead of it by 5′→3′ exonuclease activity and synthesizing t作者: 不利 時間: 2025-3-29 11:58
DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sectionshe ability of terminal deoxyribonucleotidyl transferase to add nucleotides to breaks in DNA, and has generally been termed TUNEL (terminal dUTP nick end labeling), although ISEL (. end labeling) would be a more appropriate description. During the late stages of apoptosis, nucleases are activated tha作者: Offset 時間: 2025-3-29 17:03
Detection of Specific Double-Strand DNA Breaks and Apoptosis , Using T4 DNA LigaseYet historically, the labeling of DNA breaks . was most often limited to the identification of apoptotic cells. Consequently, the major techniques for analysis of DNA cleavage in tissue sections were initially developed for the visualization of apoptotic death. These assays rely on enzymatic labelin作者: ESPY 時間: 2025-3-29 19:58 作者: BUCK 時間: 2025-3-30 01:52
The Comet Assay applications in genototoxicity testing, human biomonitoring and molecular epidemiology, ecotoxicology, as well as fundamental research in DNA damage and repair. The assay attracts adherents by its simplicity, sensitivity, versatility, speed and economy, and the number of publications it spawns rise作者: AWRY 時間: 2025-3-30 04:59
The Comet Assayimplest form, cells are embedded in agarose on a microscope slide, immersed in a lysis solution to remove lipids and proteins, and exposed to a weak electric field to attract broken, negatively-charged DNA towards the anode. After electrophoresis, DNA is stained using a fluorescent dye, and viewed u作者: 一大群 時間: 2025-3-30 11:59
Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assaysl science. A variety of experimental techniques including alkaline sucrose gradient centrifugation, alkaline elution, nucleoid sedimentation, viscoelastic measurements of DNA, alkaline unwinding, and gel electrophoresis (.–.) have been developed to detect DNA damage in large cell populations. Howeve作者: 眨眼 時間: 2025-3-30 14:20
1064-3745 Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluoresc978-1-61737-272-8978-1-59259-179-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: maintenance 時間: 2025-3-30 19:25 作者: Ingredient 時間: 2025-3-30 22:51 作者: 不連貫 時間: 2025-3-31 01:46 作者: 宿醉 時間: 2025-3-31 06:40
Quantitative Differentiation of Both Free 3′ OH and 5′ OH DNA Ends Using Terminal Transferase-Based .,.). Recently, Sahara et al. reported the discovery of a caspase-3-activated protein that is required for apoptotic chromatin condensation without DNA fragmentation (.). Therefore, chromatin condensation and DNA fragmentation are independent events of apoptosis. Both agarose gel electrophoresis usi
