標(biāo)題: Titlebook: Immunocytochemical Methods and Protocols; Lorette C. Javois Book 19951st edition Humana Press 1995 [打印本頁(yè)] 作者: 萌芽的心 時(shí)間: 2025-3-21 17:03
書目名稱Immunocytochemical Methods and Protocols影響因子(影響力)
書目名稱Immunocytochemical Methods and Protocols影響因子(影響力)學(xué)科排名
書目名稱Immunocytochemical Methods and Protocols網(wǎng)絡(luò)公開(kāi)度
書目名稱Immunocytochemical Methods and Protocols網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書目名稱Immunocytochemical Methods and Protocols被引頻次
書目名稱Immunocytochemical Methods and Protocols被引頻次學(xué)科排名
書目名稱Immunocytochemical Methods and Protocols年度引用
書目名稱Immunocytochemical Methods and Protocols年度引用學(xué)科排名
書目名稱Immunocytochemical Methods and Protocols讀者反饋
書目名稱Immunocytochemical Methods and Protocols讀者反饋學(xué)科排名
作者: Lasting 時(shí)間: 2025-3-21 20:56
Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration,um sulfate fractionation, is generally employed as the initial step in the isolation of crude antibodies from serum or ascitic fluid (.#x2013;.). Ammonium sulfate precipitation, in many instances, is still the method of choice because it offers a number of advantages. Ammonium sulfate fractionation 作者: 嬉耍 時(shí)間: 2025-3-22 03:56 作者: Thyroiditis 時(shí)間: 2025-3-22 08:09
Purification of Antibodies Using Affinity Chromatography,is type of separation, unlike other chromatographic methods, uses the intrinsic biological activity of a molecule to bind to a substrate, hapten, or antigen. Principles of matrix selection, gel preparation, and coupling of ligands have been reviewed extensively by Ostrove (.). Antibody affinity chro作者: 潛伏期 時(shí)間: 2025-3-22 11:23 作者: albuminuria 時(shí)間: 2025-3-22 16:21
Conjugation of Fluorochromes to Antibodies,t first, the specific antibody itself was labeled and applied to the tissue section to identify the antigenic sites (direct method) (.). Later, the more sensitive and versatile indirect method (.) was introduced. The primary, unlabeled, antibody is applied to the tissue section, and the excess is wa作者: 裁決 時(shí)間: 2025-3-22 19:35
Biotinylation of Antibodies,ty chromatography, and many other areas (.) (.. and .). It was first exploited in immunocytochemical applications in the mid-1970s (.,.), and has since been commonly used to localize antigens in cells and tissues. In this technique, a biotinylated primary or secondary antibody is first applied to th作者: Infantry 時(shí)間: 2025-3-23 01:06 作者: 集合 時(shí)間: 2025-3-23 05:24 作者: 托人看管 時(shí)間: 2025-3-23 08:41
Processing of Cell-Touch Preparations,ing into them precludes the use of frozen sections. Touch preparations or imprints enable the examination of the whole cell apart from the tissue aspect. The cells are obtained by touching a wet tissue with a glass slide. Cells adhere to the glass in roughly the same orientation as they exist on the作者: 廚房里面 時(shí)間: 2025-3-23 09:45
Processing of Cytological Specimens,nclude blood smears, aspirates, and swabs from any cellular site. Each sample is treated differently, and yet all the methods are interchangeable. There is no one way in which to prepare these types of cell samples for immunocytochemical analysis. This chapter will deal with the most common forms of作者: 補(bǔ)助 時(shí)間: 2025-3-23 16:16
Processing of Tissue-Culture Cells,s of cell culture that conditions can be manipulated in order to examine living cells and to understand better their behavior (.). The fact that transformed or malignant cells grow very nicely in rather simple conditions of chemical formulation make these cells ideal laboratories in which to study c作者: Amendment 時(shí)間: 2025-3-23 20:53 作者: Reclaim 時(shí)間: 2025-3-24 01:50
Overview of Fluorophores,tibody to antigen. Fluorescent molecules, termed fluorophores or fluorochromes, can be conjugated directly to antibodies by covalent linkage, or coupled indirectly to antibodies via conjugation to proteins A and G or through an avidin-biotin bridge (..,.,., and .). The basic features of immunofluore作者: 逗它小傻瓜 時(shí)間: 2025-3-24 06:23
Direct Immunofluorescent Labeling of Cells,gated antibody (., .). Although this was not the first published report of a chemically modified antibody, it was the first time the antibody was used as a tool to examine an antigen . Shortly thereafter, this method was adapted, giving rise to the indirect labeling technique (.). Rather than conjug作者: cancer 時(shí)間: 2025-3-24 08:20 作者: CODE 時(shí)間: 2025-3-24 13:57
Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells,on a substratum, the cell’s cytoplasm can be spread over a large surface area, resulting in a thin, broad cytoplasmic layer. In this layer, optical methods can resolve details in much the same way as can be accomplished by sectioning of embedded cells, but without the need for embedding and sectioni作者: 協(xié)議 時(shí)間: 2025-3-24 16:02
Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts,the peripheral nervous system. Tissues were either viewed directly, if sufficiently thin, or further separated into layers by acid maceration (.). Initially, histochemical staining, particularly methylene blue (.), was applied to whole-mounts to characterize neuronal cell types, distributions, and i作者: engender 時(shí)間: 2025-3-24 22:13
Overview of Antigen Detection Through Enzymatic Activity,n with the peroxidase enzyme rather than with a fluorescent compound (.). The difficulty with this approach lies in attaching a relatively large molecule, like an enzyme, to another large molecule, like an immunoglobulin, with neither molecule losing the ability to function. In this case, labeling c作者: 山羊 時(shí)間: 2025-3-25 02:47
ith uniform velocity. These formulae had been developed previously in Chapter 3, from Coulomb’s law taking the transformations of the theory of special relativity as axiomatic. This approach is the reverse of the historical approach. The theory of special relativity evolved from classical optics and作者: 猛烈責(zé)罵 時(shí)間: 2025-3-25 04:31 作者: 倔強(qiáng)一點(diǎn) 時(shí)間: 2025-3-25 08:41 作者: laceration 時(shí)間: 2025-3-25 13:28
Ute M. Kentthematical background material, making this textbook self-co.Scheck’s successful textbook presents a comprehensive treatment, ideally suited for a one-semester course. The textbook describes Maxwell‘s equations first in their integral, directly testable form, then moves on to their local formulation作者: 阻塞 時(shí)間: 2025-3-25 19:32
Ute M. Kentl background material is provided, making this textbook self.The book describes Maxwell‘s equations first in their integral, directly testable form, then moves on to their local formulation. The first two chapters cover all essential properties of Maxwell‘s equations, including their symmetries and 作者: macabre 時(shí)間: 2025-3-25 19:58
Ute M. Kenterpretation only in relation to quantum mechanics of electrons and the Schr?dinger equation. In this chapter, we study the generalization of the concept of a locally invariant gauge theory to non-Abelian gauge groups constructed by following the model of Maxwell theory. This generalization may seem 作者: Anonymous 時(shí)間: 2025-3-26 00:31 作者: 教義 時(shí)間: 2025-3-26 05:06 作者: surmount 時(shí)間: 2025-3-26 11:44 作者: 結(jié)果 時(shí)間: 2025-3-26 15:10 作者: 音的強(qiáng)弱 時(shí)間: 2025-3-26 16:59 作者: 責(zé)難 時(shí)間: 2025-3-26 23:03
Ute M. Kent chapter 6 gives a concise summary of semi-Riemannian geometry as the framework for the classical field theory of gravitation. The chapter concludes with a discussion of the Schwarzschild solution of Einstein‘s978-3-662-58561-0978-3-662-55579-8Series ISSN 1868-4513 Series E-ISSN 1868-4521 作者: FOLLY 時(shí)間: 2025-3-27 04:27
Ute M. Kentitation. The chapter concludes with a discussion of the Schwarzschild solution of Einstein‘s equations and the classical tests of general relativity (perihelion precession of Mercury, and light deflection by th978-3-642-43128-9978-3-642-27985-0Series ISSN 1868-4513 Series E-ISSN 1868-4521 作者: insipid 時(shí)間: 2025-3-27 06:33
Su-Yau Maoture works continuously to further the development of tools for the production of books and on the related technologies to support the authors..978-3-658-32037-9978-3-658-32038-6Series ISSN 2197-6708 Series E-ISSN 2197-6716 作者: 魯莽 時(shí)間: 2025-3-27 10:50 作者: 憲法沒(méi)有 時(shí)間: 2025-3-27 16:38
ht as his second postulate. (It is shown by Rosser., that, if it is assumed that Maxwell’s equations, are correct and obey the principle of relativity, the principle of the constancy of the speed of light follows.) After developing the Lorentz transformations, Einstein. went on to show that the Lore作者: 表示向前 時(shí)間: 2025-3-27 21:51
Su- Yau Mao,Lorette C. Javois,Ute M. Kentht as his second postulate. (It is shown by Rosser., that, if it is assumed that Maxwell’s equations, are correct and obey the principle of relativity, the principle of the constancy of the speed of light follows.) After developing the Lorentz transformations, Einstein. went on to show that the Lore作者: 有特色 時(shí)間: 2025-3-28 01:24 作者: 似少年 時(shí)間: 2025-3-28 06:07 作者: 服從 時(shí)間: 2025-3-28 09:58 作者: 加入 時(shí)間: 2025-3-28 12:41 作者: cardiopulmonary 時(shí)間: 2025-3-28 15:44 作者: recession 時(shí)間: 2025-3-28 22:06
Purification of Antibodies Using Protein A-Sepharose and FPLC,reagent for isolating immune complexes or immunoglobulins from a crude solution (.,.). Protein A has also been useful for separating Fc fragments from Fab fragments after proteolytic digestion. The advantages of protein A are mainly its stability and specificity. Protein A is stable over a wide pH r作者: 箴言 時(shí)間: 2025-3-29 02:41
Preparation of Frozen Sections for Analysis,mens, there is always a bit of denaturation as the sections are being prepared since the cut section thaws in order to adhere to the glass slide. There is also the risk of lyophilization of important components as well as the threat of freeze/thaw conditions occurring in the freezer after the specim作者: Aerophagia 時(shí)間: 2025-3-29 05:32 作者: amphibian 時(shí)間: 2025-3-29 08:45
Processing of Cytological Specimens,dilute medium are best suited for the preparation of cytospins through cytocentrifugation. Cell suspensions that exist in a high-viscosity medium are best suited to be tested as swab preparations (.). The constant among these preparations is that the whole cell is present on the slide surface. For a作者: acolyte 時(shí)間: 2025-3-29 13:20 作者: 鳥(niǎo)籠 時(shí)間: 2025-3-29 17:41 作者: 緯線 時(shí)間: 2025-3-29 22:41
Direct Immunofluorescent Labeling of Cells,fluorescence is weak since only one labeled primary antibody binds to each antigen. The indirect labeling technique adds one more incubation and wash step, but it has the advantage of amplifying the fluorescent signal because several fluorescently labeled secondary antibodies can bind to each primar作者: 新字 時(shí)間: 2025-3-30 02:19
Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells,ighly useful diagnostic tools in clinical medicine. The identification of surface antigenic molecules using antibodies can be performed by several methods, including immunofluorescence microscopy, enzyme and discrete marker microscopy, enzyme and radioactive labeling of mass cultures, and flow cytom作者: 漸強(qiáng) 時(shí)間: 2025-3-30 06:36
Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells, than an hour by immunofluorescence, a result that might take weeks using cell fractionation methods (.,.). On the other hand, there are several important methodological considerations that affect the accuracy and interpretability of the results.作者: 凝結(jié)劑 時(shí)間: 2025-3-30 10:41
Conjugation of Fluorochromes to Antibodies,ed. The primary antigenic site is thus revealed. A major advantage of the indirect method is the enhanced sensitivity. In addition, a labeled secondary antibody can be used to locate any number of primary antibodies raised in the same animal species without the necessity of labeling each primary antibody.作者: interpose 時(shí)間: 2025-3-30 12:48 作者: 省略 時(shí)間: 2025-3-30 18:09 作者: PTCA635 時(shí)間: 2025-3-30 23:55
Overview of Cell Fixation and Permeabilization,d proteins and carbohydrates. This chapter will review some of the advantages and drawbacks of common chemical fixation and permeabilization methods used for immunolocalization at the level of light microscopy.作者: Irritate 時(shí)間: 2025-3-31 02:21 作者: 解脫 時(shí)間: 2025-3-31 06:25
Book 19951st editionring this time the use of immunocytochemical meth- ods has spread to a wide array of biological disciplines. Early applica- tions focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic d作者: maroon 時(shí)間: 2025-3-31 09:43 作者: 機(jī)密 時(shí)間: 2025-3-31 16:33 作者: 抵制 時(shí)間: 2025-3-31 17:32 作者: 兇殘 時(shí)間: 2025-4-1 00:54
