作者: Lineage 時間: 2025-3-21 22:23 作者: nostrum 時間: 2025-3-22 03:02 作者: enlist 時間: 2025-3-22 06:56 作者: 格子架 時間: 2025-3-22 12:11 作者: –吃 時間: 2025-3-22 15:31
Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase,?hler and Milstem (.) These workers have shown that lymphocytes can be immortalized and subsequently cultured after so1atic fusion with genetically selected myeloma cells. Usually, once fusion between spleen cells and myeloma cells has been performed, cells are suspended in a large volume of selecti作者: fluoroscopy 時間: 2025-3-22 17:27
Synthesis of Peptides for Use as Immunogens, cells or tissues. With the advent of recombinant DNA technology, it is now often possible to deduce the primary amino acid sequence of a polypeptide without its purification. Two strategies then exist to raise appropriate antibodies. The gene can be expressed in a heterologous species, usually bact作者: 火花 時間: 2025-3-22 22:31 作者: forbid 時間: 2025-3-23 03:08 作者: Perigee 時間: 2025-3-23 06:14
IgG Purification,e supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result can often be obtained if some form of enrichment or isolation of immunoglobulin (Ig) is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity c作者: 繞著哥哥問 時間: 2025-3-23 13:15
Purification of Monoclonal Antibodies,teristics that can be produced in large amounts from immortal cell lines. MAbs can be exquisitely specific, but preparations containing these may be contaminated with tissue-culture additives and nonimmunoglobulin secretion products when produced in vitro, and by host animal proteins when prepared a作者: implore 時間: 2025-3-23 16:02
Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives,heir application in a variety of diagnostic situations that require the crosslinking of two protein surfaces, for example, antigen and detecting agent in lmmunohis-tochemistry or immunoassays. More interestingly, BsAbs have been shown to have considerable therapeutic potential when used as targeting作者: Arthropathy 時間: 2025-3-23 19:46 作者: calamity 時間: 2025-3-24 00:11
,Immunoaffinity Purification and Quantification of Antibody—Toxin Conjugates,.,. and Chapter 13). Gel permeation chromatography is used as a first step to purify conjugate molecules from the reaction mixture. This procedure removes protein aggregates, the excess of RIP employed in the conjugation reaction, and low molecular weight byproducts. However, a significant fraction 作者: Consensus 時間: 2025-3-24 04:47
Competitive ELISA,bodies, monoclonal or polyclonal, to the compounds are available. The technique has found particular application in the monitoring of environmental contaminants and toxins, either studying the primarily contaminated materials, e.g., foodstuffs, or body fluids of potentially exposed humans. The techn作者: Barrister 時間: 2025-3-24 07:48 作者: stress-test 時間: 2025-3-24 13:35 作者: Libido 時間: 2025-3-24 15:02 作者: FOR 時間: 2025-3-24 19:38 作者: Engaged 時間: 2025-3-25 02:51 作者: 釋放 時間: 2025-3-25 03:21
Jonathan A. Green,Margaret M. Mansony material: I am very pleased to have been asked to write the foreword to this book. The technical advances in diagnostic radiology in the last few decades have transformed clinical practice and have been nothing short of astonishing. The subject of diagnostic radiology is now very large and radiolo作者: 妨礙 時間: 2025-3-25 10:42 作者: Pedagogy 時間: 2025-3-25 13:25 作者: LIMN 時間: 2025-3-25 17:21
Christopher J. Deanransformed clinical practice and have been nothing short of astonishing. The subject of diagnostic radiology is now very large and radiology depa- ments are involved in all areas of modern patient care.The defining event in m- ern radiology,and arguably the most significant development in radiology 作者: BOOM 時間: 2025-3-25 22:33 作者: SCORE 時間: 2025-3-26 02:26 作者: 歡樂中國 時間: 2025-3-26 06:10 作者: conspicuous 時間: 2025-3-26 11:24
Boguslaw Szewczyk,Donald F. Summersmption as a primary criterion of market efficiency. When private decisions exert effects that are external to the decision-maker, “ideal” output is not obtained through the competitive organisation of economic activity even if the remaining conditions necessary for efficiency are satisfied. The mark作者: 百科全書 時間: 2025-3-26 13:04
Boguslaw Szewczyk,Donald F. Summersitation. The feeling that these products are now too cheap for the good of future generations, that they are being selfishly exploited at too rapid a rate, and that in consequence of their excessive cheapness they are being produced and consumed wastefully has given rise to the conservation movement作者: 有雜色 時間: 2025-3-26 19:55 作者: 卵石 時間: 2025-3-27 00:28 作者: cartilage 時間: 2025-3-27 02:55 作者: organic-matrix 時間: 2025-3-27 07:22 作者: DALLY 時間: 2025-3-27 10:42 作者: ENNUI 時間: 2025-3-27 15:14
Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins,ncreasingly for expression of glycoproteins, because the recombinant material is glycosylated. It should be remembered, however, that glycosylation may be species-specific and if one of the functions of the protein depends on carbohydrate, then the function of a recombinant glycoprotein may be alter作者: overhaul 時間: 2025-3-27 19:33 作者: Spangle 時間: 2025-3-27 22:02
Synthesis of Peptides for Use as Immunogens, antibody is already well defined (.,.) In this way, antibodies can be raised against novel gene products that are specifically directed against sites of interest, for example, unique regions, highly conserved regions, active sites, or extracellular or intracellular domains. Moreover, the ready avai作者: PHAG 時間: 2025-3-28 02:57
IgG Purification,e supernatant or the isolation of a particular antigen-binding fraction of Ig from such fluids. The former can be achieved by biochemical procedures, whereas the latter usually requires some type of affinity purification.作者: 放棄 時間: 2025-3-28 08:17
Epitope Mapping,cted, and may depend also on the mapping method used. Epitope mapping is usually done with MAbs, though it can be done with polyclonal antisera in a rather less rigorous way, bearing in mind that antisera behave as a mixture of MAbs of variable complexity. Some authors have extended the epitope conc作者: 戰(zhàn)勝 時間: 2025-3-28 10:32
Coupling of Antibodies with Biotin,y of mannose and glucosamine (.), avidin can interact nonspecifically with negative charges on cell surfaces and nucleic acids, or with membrane sugar receptors. At times, this causes background problems in histochemical and cytochemical applications. Streptavidin, a near-neutral, biotin-binding pro作者: Feature 時間: 2025-3-28 18:25 作者: Introvert 時間: 2025-3-28 20:19 作者: 六邊形 時間: 2025-3-28 23:04 作者: 變形詞 時間: 2025-3-29 04:01 作者: 清唱劇 時間: 2025-3-29 07:43 作者: Irksome 時間: 2025-3-29 11:27 作者: NAIVE 時間: 2025-3-29 19:33 作者: Synthesize 時間: 2025-3-29 22:51 作者: EWER 時間: 2025-3-30 01:36
David C. Hancock,Gerard I. Evanhe various sources of these publications. Change is produced by the interactions between the variousdis- plines. The editors have had a very difficult task in selecting the key discoveries and descriptions.The radiological literature is very large.Medical imaging continues to develop rapidly and the作者: 感染 時間: 2025-3-30 05:36 作者: ALTER 時間: 2025-3-30 08:44 作者: 極少 時間: 2025-3-30 13:23
David C. Hancock,Nicola J. O’ Reilly,Gerard I. Evanon, as have also closed seasons for fish and game and statutes forbidding certain highly efficient means of catching fish. Taxation would be a more economic method than publicly ordained inefficiency in the case of purely commercial activities such as mining and fishing for profit, if not also for s作者: 變態(tài) 時間: 2025-3-30 18:07 作者: milligram 時間: 2025-3-30 21:27 作者: NUL 時間: 2025-3-31 02:53
Mark Page,Robin Thorpeconomics, namely, the implication that externalities are either reduced or eliminated by the shift of an activity from market to political organisation. I shall try to show that this implication will stand up to critical scrutiny only under certain highly restricted assumptions about human behaviour作者: 鉤針織物 時間: 2025-3-31 05:18 作者: Champion 時間: 2025-3-31 12:04
Rosaria P. Haugland,Mahesh K. Bhalgatd to mature, interest has increased in tracing the early papers that have served as the foundations of the field. Cheret [Shock Compression of Condensed Matter - 1989, Elsevier Sci. Pub. B. V. , pp 11-19, 1990) has contributed to this literature with his review of the life of Hugoniot on the one-hun作者: radiograph 時間: 2025-3-31 15:18
,A Simple Hollow-Fiber Bioreactor for the “In-House” Production of Monoclonal Antibodies,pernatant and is expensive. This chapter describes a simple method for culturmg hybridomas in vitro in renal dialysis cartridges, which allows several hundred milligrams of MAb to be recovered at high concentration.作者: 到婚嫁年齡 時間: 2025-3-31 21:17 作者: 征服 時間: 2025-3-31 23:02
Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives, reagents to deliver pharmacological substances (drugs or toxins) or cellular effectors (T-cells, monocytes, or NK cells) to tumor cells (.,.). A relatively new development is the design of BsAbs to deliver chelated radiometals for the diagnostic imaging of tumors and for radioimmunotherapy (.).作者: Duodenitis 時間: 2025-4-1 05:16
Production of Polyclonal Antisera,idity may be suitable for immunohistochemistry, where it is essential that the antibody remains attached during the extensive washing procedures, but may be less useful for lmmunoaffinity chromatography, as it may prove impossible to elute the antigen from the column without extensive denaturation.
