標(biāo)題: Titlebook: Immunochemical Protocols; Margaret M. Manson Book 19921st edition Humana Press 1992 [打印本頁(yè)] 作者: 淹沒(méi) 時(shí)間: 2025-3-21 18:16
書目名稱Immunochemical Protocols影響因子(影響力)
作者: Acupressure 時(shí)間: 2025-3-21 20:58 作者: 微枝末節(jié) 時(shí)間: 2025-3-22 01:47
Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins,systems are being used increasingly for expression of glycoproteins because the recombinant material is glycosylated. A number of protocols will be described here that we have used successfully with both rat (Y3 and IR983F) and mouse (SP2/0) myelomas to generate MAbs to recombinant material or peptides based on cDNA sequences.作者: inconceivable 時(shí)間: 2025-3-22 08:27
Immunogold Probes for Light Microscopy,nt technique, are all routinely used for immunohistochemistry. Recently, the use of colloidal gold probes as immunocytochemical markers has been described for electron immunocytochemistry (.), and these are now proving to be of use at the light microscope level.作者: occurrence 時(shí)間: 2025-3-22 12:45 作者: inhibit 時(shí)間: 2025-3-22 16:19 作者: 中古 時(shí)間: 2025-3-22 17:51 作者: precede 時(shí)間: 2025-3-23 00:11
Immunogold Probes in Electron Microscopy,on microscopy is exactly that which damages antigens with respect to reaction with specific antibody. Cell preparation for electron microscopy is therefore a compromise between retaining sufficient antigenicity while preserving the cell ultrastructure.作者: gerrymander 時(shí)間: 2025-3-23 04:36 作者: TEM 時(shí)間: 2025-3-23 08:44
Efficient Elution of Purified Proteins from Polyvinylidene Difluoride Membranes (lmmobilon) After Tgels is immense (for example, . refs. 1–5), In spite of the numerous variations in the procedure of elution, none of the available methods is entirely satisfactory. Some of the methods are very laborious, and others lead to loss of resolution or poor recovery.作者: zonules 時(shí)間: 2025-3-23 13:03 作者: 易達(dá)到 時(shí)間: 2025-3-23 16:04 作者: WAIL 時(shí)間: 2025-3-23 21:34
Edward A. Bayer,Meir Wilchek the classic papers in this collection have stood the test of time, others have gained classic status by virtue of their profound influence in relatively quick measure or because of timeliness in filling a void978-1-61737-874-4978-1-59745-428-5作者: Generalize 時(shí)間: 2025-3-24 02:16 作者: FRAX-tool 時(shí)間: 2025-3-24 03:51 作者: BALE 時(shí)間: 2025-3-24 08:16 作者: 山間窄路 時(shí)間: 2025-3-24 14:23 作者: 典型 時(shí)間: 2025-3-24 15:36 作者: 不法行為 時(shí)間: 2025-3-24 20:58
Edward A. Bayer,Meir Wilchekes the importance of a paper and its ability to retain its impact over time. While many of the classic papers in this collection have stood the test of time, others have gained classic status by virtue of their profound influence in relatively quick measure or because of timeliness in filling a void作者: Gustatory 時(shí)間: 2025-3-25 00:44
Julian E. Beesleyhe various sources of these publications. Change is produced by the interactions between the variousdis- plines. The editors have had a very difficult task in selecting the key discoveries and descriptions.The radiological literature is very large.Medical imaging continues to develop rapidly and the作者: floodgate 時(shí)間: 2025-3-25 03:44
Synthesis of Peptides for Use as Immunogens,roducts that are specifically directed against sites of interest, for example, unique regions, highly conserved regions, active sites, extracellular or intracellular domains. Moreover, the ready availability of the pure peptide immunogen against which the antibody was raised means that sera can be r作者: 同時(shí)發(fā)生 時(shí)間: 2025-3-25 07:33 作者: outer-ear 時(shí)間: 2025-3-25 13:39
Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase,umber of wells to be tested (often a few hundred), and to the small quantities of MAbs available (at best, 300 μL at a few μg/mL), it is not easy at this stage to characterize the fine specificity of the antibodies (i.e., recognition of a precise epitope, inhibitory effect on a biological system, pr作者: Manifest 時(shí)間: 2025-3-25 17:13
Purification of Immunoglobulin G (IgG),tant, or the isolation of a particular antigen binding fraction from such fluids. The former can be achieved by biochemical procedures, whereas the latter usually requires some form of affinity purification.作者: ONYM 時(shí)間: 2025-3-25 20:02
Immunohistochemical detection of BromodeoxyurIDine-Labeled Nuclei for In Vivo Cell Kinetic Studies,f tissue by light microscopy. It has also proved extremely valuable for studies in conjunction with flow cytometry and even, for in vivo studies of human tumor cell kinetics (. this vol., Chapter .). We describe here a method to detect dNA synthesis by in vivo labeling of nuclei with BrdU, followed 作者: 與野獸博斗者 時(shí)間: 2025-3-26 00:35
Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen,neous proteins, are often difficult to obtain. The main reasons for this are the small amounts of protein available after the various classical purification processes and the low purity of the proteins.作者: neurologist 時(shí)間: 2025-3-26 07:21
Electron Microscopic Silver Enhancement for Double Labeling with Antibodies Raised in the Same Specodies (Ab) to be used are raised in the same species, as is usually the case with monoclonal antibodies (MAb), the difficulty arises that the labeled secondary, antispecies Ab used in the first labeling step traps the primary Ab directed against the second antigen, thus leading to a nonspecific signal for the second antigen.作者: N斯巴達(dá)人 時(shí)間: 2025-3-26 10:14
Epitope Mapping,Monoclonal antibodies (MAbs) are specific immunological tools because they bind to a precise determinant (the epitope) on the surface of a protein. The procedure of identifying the binding site of a MAb is often termed “epitope mapping.”作者: Indolent 時(shí)間: 2025-3-26 15:21 作者: ALOFT 時(shí)間: 2025-3-26 20:50 作者: jarring 時(shí)間: 2025-3-27 00:26 作者: Androgen 時(shí)間: 2025-3-27 03:40 作者: homocysteine 時(shí)間: 2025-3-27 05:45 作者: hypnotic 時(shí)間: 2025-3-27 13:00 作者: AVID 時(shí)間: 2025-3-27 14:32 作者: V洗浴 時(shí)間: 2025-3-27 18:21 作者: 拋媚眼 時(shí)間: 2025-3-28 01:45 作者: 領(lǐng)導(dǎo)權(quán) 時(shí)間: 2025-3-28 03:29
Kurt Bienz,Denise Eggery material: I am very pleased to have been asked to write the foreword to this book. The technical advances in diagnostic radiology in the last few decades have transformed clinical practice and have been nothing short of astonishing. The subject of diagnostic radiology is now very large and radiolo作者: ligature 時(shí)間: 2025-3-28 09:18
Production of Polyclonal Antisera,sed by injecting an immunogen into an animal and, after an appropriate time, collecting the blood fraction containing the antibodies of interest. In producing antibodies, several parameters must be consIDered with respect to the final use to which the antibody will be put. These include (1) the spec作者: 受辱 時(shí)間: 2025-3-28 11:05 作者: NADIR 時(shí)間: 2025-3-28 15:42
Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen,neous proteins, are often difficult to obtain. The main reasons for this are the small amounts of protein available after the various classical purification processes and the low purity of the proteins.作者: musicologist 時(shí)間: 2025-3-28 20:15 作者: 喊叫 時(shí)間: 2025-3-29 02:50
Production and Characterization of Antibodies Against Synthetic Peptides,he methods described below are designed to give optimal results with minimal injury to the test animal, and we have used them extensively and successfully for several years (.–.). Peptide immunizations differ from those in which the immunogen is a larger macromolecule in that maximal antipeptide tit作者: Pantry 時(shí)間: 2025-3-29 05:19 作者: 相互影響 時(shí)間: 2025-3-29 10:14
Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase,?hler and Milstein (.). These workers have shown that lymphocytes can be immortalized and subsequently cultured after somatic fusion with genetically selected myeloma cells. Usually, once fusion between spleen cells and myeloma cells has been performed, cells are suspended in a large volume of selec作者: COMA 時(shí)間: 2025-3-29 11:48
Purification of Immunoglobulin G (IgG),re supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result can often be obtained if some form of enrichment or isolation of immunoglobulin is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity colum作者: 門閂 時(shí)間: 2025-3-29 19:12
Enzyme-Antienzyme Method for Immunohistochemistry,is labeled with an enzyme that can be reacted with a suitable substrate to give a colored product. The alternative is to use a fluorescent label. The advantage of an enzyme label is that the nuclei can be counterstained, thereby revealing the tissue architecture, and that the stain fades slowly, if 作者: 責(zé)問(wèn) 時(shí)間: 2025-3-29 22:45 作者: fetter 時(shí)間: 2025-3-30 03:49 作者: Abbreviate 時(shí)間: 2025-3-30 04:17
,Avidin–Biotin Technology, application (for general reviews, . refs. 1 and 2). For example, a fluorescent form of avidin can be used for fluorescence microscopy, fluorescence-activated cell sorting, and in some cases, for immunoassay. Like-wise, an avidin-enzyme conjugate can be used for immunoblotting, immunoassay, light mi作者: 鎮(zhèn)痛劑 時(shí)間: 2025-3-30 11:31
Immunochemical Applications of Avidin-Biotin Technology,sults (.,.). The antibody, bound to the column via an avidin-biotin bridge, is less affected by the chemistry of immobilization or by physical interactions (hydrophobic, electrostatic, salting-out effects, precipitation, and so on) with the solid support. Such potential deleterious effects are borne作者: ARK 時(shí)間: 2025-3-30 14:36
Immunogold Probes for Light Microscopy,gen localization. All other immunocytochemical techniques are based on the same philosophy, but use different microscopically dense markers. For instance, Avrameas and Uriel (.) and Nakane and Pierce (.) described the use of the enzyme peroxidase as a dense marker for immunocytochemistry, and this w作者: 臥虎藏龍 時(shí)間: 2025-3-30 18:43 作者: collagen 時(shí)間: 2025-3-30 21:19
Electron Microscopic Silver Enhancement for Double Labeling with Antibodies Raised in the Same Specodies (Ab) to be used are raised in the same species, as is usually the case with monoclonal antibodies (MAb), the difficulty arises that the labeled secondary, antispecies Ab used in the first labeling step traps the primary Ab directed against the second antigen, thus leading to a nonspecific sign作者: Euthyroid 時(shí)間: 2025-3-31 01:21
A Recursive Least Squares Solution for Recovering Robust Planar Homographiesm its ability in dealing with corrupted and periodic measurements to provide the best solution. Furthermore, its capacity for providing reliable results for time varying parameter estimation also motivates its use in the context of real time cooperative image mosaicing where optimal transformation b作者: 信任 時(shí)間: 2025-3-31 06:28
2194-1009 ves as an anthology of research with acommon basis?in algebra, functional analysis and their applications.Special attention is devoted to?non-commutative algebras, non-associativealgebras, operator theory and ring and module theory.?These themes arerelevant in research and development in coding theo作者: JEER 時(shí)間: 2025-3-31 12:13
https://doi.org/10.1007/978-1-4612-1632-2 Lagerstelle gerecht wird, anderseits unter diesen Bedingungen eine minimale Reibung aufweist. Dies führt in der Anwendung selbst nochmals zu einer merklichen Effizienzsteigerung und global zu einer weiteren Reduktion der CO.-Emission.作者: Restenosis 時(shí)間: 2025-3-31 16:17
A Multi-agent Home Automation System for Power Managementrgency mechanism, which protects from constraint violations, and an anticipation mechanism, which computes the best future set-points according to predicted consumptions and productions and to user criteria. The paper details a negotiation protocol used by the both mechanisms and presents some preliminary simulation results.作者: 壟斷 時(shí)間: 2025-3-31 17:34
https://doi.org/10.1057/9780230590526n of EMI effects are based on primitive power grid infrastructure. Smart Grid is itself a developing technology which demands painstaking research endeavors. Safety of Smart Grid infrastructure is a crucial issue to be addressed due to its heterogeneous infrastructure. In this paper, the possible el作者: 裂縫 時(shí)間: 2025-3-31 22:21
Jiaqi Guo,Xiao Zhang,Junhua Xiao,Nan Wu with the highest scores by the VISIGRAPP Program Committee members and then they were extended and revised by the authors. Special thanks go to all contributors and re- rees, without whom this book would not have been possible. VISIGRAPP 2007 included four invited keynote lectures, presented by int作者: 改革運(yùn)動(dòng) 時(shí)間: 2025-4-1 03:15 作者: 嚴(yán)重傷害 時(shí)間: 2025-4-1 09:35 作者: 無(wú)孔 時(shí)間: 2025-4-1 11:03
Discrete Mathematics and Theoretical Computer Science4th International Co作者: septicemia 時(shí)間: 2025-4-1 14:46
Johannes Bilstein,Jutta EcariusAuf dem Weg zu einem neuen Bildungskanon作者: 誘騙 時(shí)間: 2025-4-1 20:07
