作者: 鬧劇 時(shí)間: 2025-3-21 20:15 作者: 你不公正 時(shí)間: 2025-3-22 01:23 作者: Monocle 時(shí)間: 2025-3-22 04:51
Raghav Rajagopalanrate tissue-specific cell lineage reporters, overexpress genes from a defined locus, and introduce and repair single point mutations in hPSCs. This ability to genetically engineer pluripotent stem cells will significantly facilitate the study of human disease in a defined genetic context. Here we ou作者: 帽子 時(shí)間: 2025-3-22 11:02
Raghav Rajagopalanreby generating Ty5 elements with new target specificities. The mechanism of Ty5 target site choice suggests that integration specificity of other retrotransposons and retroviruses can be altered by engineering integrases to recognize DNA-bound protein partners. Retroelements can also be used to pro作者: Heresy 時(shí)間: 2025-3-22 12:55 作者: constitute 時(shí)間: 2025-3-22 19:43 作者: Camouflage 時(shí)間: 2025-3-22 21:27
Raghav Rajagopalanes a simple antibiotic selection to isolate cell lines in which the introduced plasmid has integrated at the desired . site. A polymerase chain reaction assay is also presented to verify correct chromosomal placement of the introduced plasmid. This integration system based on ?C31 integrase supplies作者: Small-Intestine 時(shí)間: 2025-3-23 02:20 作者: 剝削 時(shí)間: 2025-3-23 07:25 作者: 試驗(yàn) 時(shí)間: 2025-3-23 11:47
1568-2846 e. Each of these systematically harnesses knowing using both modes of consciousness. Therefore, the author insists, such approaches must be included in systemic practice, in purposeful and methodical juxtaposit978-3-030-49137-6978-3-030-49135-2Series ISSN 1568-2846 作者: magnate 時(shí)間: 2025-3-23 15:55 作者: STAT 時(shí)間: 2025-3-23 19:16 作者: NOVA 時(shí)間: 2025-3-24 01:34 作者: chance 時(shí)間: 2025-3-24 05:30
https://doi.org/10.1007/978-3-030-49135-2Immersive Systemic Knowing; Systems Thinking; Systemic Philosophy; Systemic Ontology; Extended Epistemol作者: TAIN 時(shí)間: 2025-3-24 09:44 作者: Feigned 時(shí)間: 2025-3-24 11:50 作者: 規(guī)章 時(shí)間: 2025-3-24 15:35 作者: gustation 時(shí)間: 2025-3-24 21:07 作者: CLAP 時(shí)間: 2025-3-25 00:20
Immersive Systemic Knowing: A Theory for Knowing in Practice, . immersive systemic knowing framework作者: 排出 時(shí)間: 2025-3-25 06:35 作者: 取之不竭 時(shí)間: 2025-3-25 11:09 作者: 侵略者 時(shí)間: 2025-3-25 12:06
Immersive Systemic Knowing978-3-030-49135-2Series ISSN 1568-2846 作者: 焦慮 時(shí)間: 2025-3-25 18:55 作者: 多余 時(shí)間: 2025-3-25 22:11
Raghav Rajagopalans and dose rates, however, still remain ambiguous (Pohl-Rüling 1990; Sagan 1987). The study of interaction of low dose irradiation with subsequent mutagenic treatment could be a way of evaluating the effects of low doses of irradiation. This type of research has linked to a general biological phenom作者: 愉快么 時(shí)間: 2025-3-26 03:03 作者: indifferent 時(shí)間: 2025-3-26 06:45
Raghav Rajagopalan, 2007), collectively referred to as pluripotent stem cells (hPSCs), are currently used in disease modeling to address questions specific to humans and to complement our insight gained from model organisms (Soldner et al. Cell 146:318–331, 2011; Soldner and Jaenisch, Science 338:1155–1156, 2012). Re作者: interrogate 時(shí)間: 2025-3-26 09:27
Raghav Rajagopalan retroelement of specific chromatin states or DNA-bound protein complexes. A well-studied example of such a targeted retroelement is the . Ty5 retrotransposon, which integrates into heterochromatin at the telomeres and silent mating loci. Targeting is mediated by an interaction between Ty5 integrase作者: PACT 時(shí)間: 2025-3-26 15:00 作者: MEEK 時(shí)間: 2025-3-26 16:47
Raghav Rajagopalanbination in ., these protocols have reduced dependence on restriction endonucleases, allowing the fabrication of complex TV constructs with relative ease. Using a methodology based on phage-plasmid recombination, we have developed a comprehensive TV construction protocol dubbed Orpheus recombination作者: oncologist 時(shí)間: 2025-3-26 23:07
Raghav Rajagopalanise, unidirectional recombination between two attachment or . sites called . and .. We have shown that an . site preintegrated into a mammalian chromosome can serve as a target for integration of an introduced plasmid carrying an . site. Recombination leads to precise integration of the plasmid into作者: 繁殖 時(shí)間: 2025-3-27 04:43 作者: Charitable 時(shí)間: 2025-3-27 07:42 作者: 比喻好 時(shí)間: 2025-3-27 10:21
1568-2846 fers a comprehensive examination of systems philosophy, hithThis book advances systems thinking by introducing a new philosophy of systemic knowing. It argues that there are inescapable limits to rational understanding. Humankind has always depended on extended ways of knowing to complement the rati作者: antenna 時(shí)間: 2025-3-27 14:52
Book 2020standing. Humankind has always depended on extended ways of knowing to complement the rational-analytic approach. The book establishes that the application of such methods is fundamental to systemic practice.?.The author advocates embracing two modes of consciousness: intentionality, which Western p作者: 漸變 時(shí)間: 2025-3-27 20:01 作者: 是他笨 時(shí)間: 2025-3-27 22:49
