作者: CLAIM 時(shí)間: 2025-3-21 23:30
CRISPR-Cas9-Mediated Gene Silencing in Cultured Human Epithelia,f cell delivery, and (3) the possibility to do multigene editing. Here, we describe a method to introduce single gene disruption in lung bronchial epithelial cells. This approach can be used to study host factors important for pathogen interaction or to identify and study genetic markers determining susceptibility to fungal disease.作者: 多骨 時(shí)間: 2025-3-22 01:11
A Murine Model for Chronic , Airway Infections,cheally infected with . conidia embedded in agar beads. This approach results in the establishment of hyphal infection within the airways of mice for up to a 28-day period and is amenable to the study of innate and adaptive antifungal responses, fungal mutant strains, and antifungal agents.作者: 蠟燭 時(shí)間: 2025-3-22 06:20
Book 2021 the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge,?.Host-Fungal Interactions: Methods and Protocols .aims to ensure successful results in the further study of this vital field..作者: Graduated 時(shí)間: 2025-3-22 12:13 作者: meritorious 時(shí)間: 2025-3-22 12:54
Book 2021se. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfall作者: 哀悼 時(shí)間: 2025-3-22 20:38 作者: STALE 時(shí)間: 2025-3-22 22:53 作者: 裂縫 時(shí)間: 2025-3-23 05:08 作者: 內(nèi)閣 時(shí)間: 2025-3-23 08:42 作者: B-cell 時(shí)間: 2025-3-23 10:30 作者: 外貌 時(shí)間: 2025-3-23 16:15
,Fungal Bioreporters to Monitor Outcomes of ,: Host–Cell Interactions,o interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the filamentous mold 作者: persistence 時(shí)間: 2025-3-23 18:31 作者: 苦笑 時(shí)間: 2025-3-24 02:06
Measuring In Vivo Neutrophil Trafficking Responses During Fungal Infection Using Mixed Bone Marrow vo, using mixed bone marrow chimeras and flow cytometry. This method directly assesses the relative contribution of a receptor or intracellular molecule in controlling neutrophil chemotaxis during fungal infection and can be adapted to a variety of other non-fungal infection experimental settings.作者: Repetitions 時(shí)間: 2025-3-24 06:24 作者: apropos 時(shí)間: 2025-3-24 06:48 作者: 使厭惡 時(shí)間: 2025-3-24 12:08 作者: Metamorphosis 時(shí)間: 2025-3-24 15:10 作者: LAPSE 時(shí)間: 2025-3-24 22:51
Spyridoula Nikou,Jemima Ho,Olivia Hepworth,Nicole Ponde,Ruth Dickenson,Jonathan P. Richardson,Julianinclude experiments in organic and physical chemistry. Later Eliot chaired the ‘Committee of Ten’ which encouraged all schools to make practical chemistry an essential part of chemistry curricula in the United States. The story is thus of the slow process of change in the teaching of chemistry over 作者: 向宇宙 時(shí)間: 2025-3-25 00:41 作者: 改良 時(shí)間: 2025-3-25 04:07 作者: tackle 時(shí)間: 2025-3-25 11:05 作者: 共同確定為確 時(shí)間: 2025-3-25 13:16
Patawee Asamaphan,Gordon D. Brown,Janet A. Willmentnforms to this conception, allowing it to proceed accurately without puzzles or paradoxes in its factfinding and allowing me to avoid calling for radical reform. Section .: The underlying multivalent logic comprises a group of valid logic systems, such as fuzzy logic and possibility theory. They are作者: etiquette 時(shí)間: 2025-3-25 19:33
Rebecca A. Drummond,Michail S. Lionakisntion of probability and on to the present. They are also reevaluating such intuitive practices as those that humans use in daily life to combine inferences upon inferences. A hotly contested literature has emerged...In a significant, comprehensive, and original contribution, this book develops a th作者: Cumbersome 時(shí)間: 2025-3-25 23:22 作者: 膽大 時(shí)間: 2025-3-26 00:21 作者: 缺陷 時(shí)間: 2025-3-26 07:19 作者: Itinerant 時(shí)間: 2025-3-26 10:49 作者: 單挑 時(shí)間: 2025-3-26 13:52 作者: Preserve 時(shí)間: 2025-3-26 17:52
Experimental Evolution of , by Serial Passaging in Host Cells,very evolution experiment is different, and these techniques should be taken as a general guideline to be adapted to different organisms and questions. Then, it is a powerful tool with many potential applications in pathobiology research.作者: infantile 時(shí)間: 2025-3-27 00:44
Quantifying Receptor-Mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells,the β-1,3 glucan receptor, Dectin-1 (CLEC7A), as an example. The use of ELISA-based assays to measure cytokine production by immune cells that are induced in response to fungi and methods for isolating and culturing primary macrophages from various murine tissues are described.作者: 可耕種 時(shí)間: 2025-3-27 04:22 作者: arterioles 時(shí)間: 2025-3-27 08:34 作者: Neuralgia 時(shí)間: 2025-3-27 10:58
categories, such emotional intentionality and emotional habits. Finally, the third part of the book concludes by drawing some consequences of the layered theory of emotions for philosophy of mind, and for the 978-3-031-63201-3978-3-031-63199-3作者: instructive 時(shí)間: 2025-3-27 16:13 作者: Accolade 時(shí)間: 2025-3-27 20:22 作者: Gratuitous 時(shí)間: 2025-3-28 00:38
https://doi.org/10.1007/978-1-0716-1182-1next gen sequencing; Nanostring; CRISPR; Macrophages; T cells作者: burnish 時(shí)間: 2025-3-28 02:11 作者: Nucleate 時(shí)間: 2025-3-28 08:29 作者: 逃避現(xiàn)實(shí) 時(shí)間: 2025-3-28 10:29
Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections,cell transplantation (HCT) is described. This model can be used to investigate the establishment and progression of fungal infections after HCT and to elucidate how different transplant variables affect the recovery of host immunity.作者: 有特色 時(shí)間: 2025-3-28 18:03 作者: 的染料 時(shí)間: 2025-3-28 19:59 作者: gastritis 時(shí)間: 2025-3-28 23:34 作者: 放肆的你 時(shí)間: 2025-3-29 03:59 作者: Protein 時(shí)間: 2025-3-29 10:49 作者: 馬籠頭 時(shí)間: 2025-3-29 14:43 作者: Dappled 時(shí)間: 2025-3-29 17:47
Analysis of Epithelial Cell Responses to Microbial Pathogens,ystander” cells with barrier function, the epithelial cell is now known to be a sentinel cell in the recognition and discrimination of commensal and pathogenic microorganisms and a key cell in initiating subsequent innate and adaptive immune responses. Here, we describe the main assays utilized in a作者: DIS 時(shí)間: 2025-3-29 20:57
Single-Cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluoreuman .irway .pithelial .ells (AECs) play a crucial role in host defense by promoting uptake and killing of inhaled microorganisms and concomitant cytokine production in order to recruit professional phagocytes to the site of infection. However, inhaled pathogens can also reside and replicate intrace作者: 橫條 時(shí)間: 2025-3-30 02:20
,Fungal Bioreporters to Monitor Outcomes of ,: Host–Cell Interactions,nters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells t作者: 搖晃 時(shí)間: 2025-3-30 06:53 作者: judiciousness 時(shí)間: 2025-3-30 09:22 作者: Affectation 時(shí)間: 2025-3-30 15:03 作者: GEN 時(shí)間: 2025-3-30 19:48
Quantifying Receptor-Mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells,macrophages, control and regulate immunity to fungi. These cellular responses are initiated when conserved pathogen components, known as pathogen-associated molecular patterns (PAMPs), are recognized by pattern-recognition receptors (PRRs), which include members of the C-type lectin receptor (CLR) f作者: 愛(ài)好 時(shí)間: 2025-3-30 23:33
Measuring In Vivo Neutrophil Trafficking Responses During Fungal Infection Using Mixed Bone Marrow he molecular factors that mediate neutrophil chemotaxis is therefore critical for our understanding of disease pathogenesis and the mechanisms underlying protective immunity. Here, we describe a protocol that enables analysis of neutrophil recruitment from the blood into fungal-infected organs in vi作者: FLAT 時(shí)間: 2025-3-31 03:12 作者: Baffle 時(shí)間: 2025-3-31 06:28
Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections,cell transplantation (HCT) is described. This model can be used to investigate the establishment and progression of fungal infections after HCT and to elucidate how different transplant variables affect the recovery of host immunity.
