標題: Titlebook: Homing Endonucleases; Methods and Protocol David R. Edgell Book 2014 Springer Science+Business Media New York 2014 Binding and cleavage spe [打印本頁] 作者: 撕成碎片 時間: 2025-3-21 18:49
書目名稱Homing Endonucleases影響因子(影響力)
書目名稱Homing Endonucleases影響因子(影響力)學科排名
書目名稱Homing Endonucleases網絡公開度
書目名稱Homing Endonucleases網絡公開度學科排名
書目名稱Homing Endonucleases被引頻次
書目名稱Homing Endonucleases被引頻次學科排名
書目名稱Homing Endonucleases年度引用
書目名稱Homing Endonucleases年度引用學科排名
書目名稱Homing Endonucleases讀者反饋
書目名稱Homing Endonucleases讀者反饋學科排名
作者: Spirometry 時間: 2025-3-21 20:49 作者: Priapism 時間: 2025-3-22 01:28
A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleasefore, it can be used for assaying in vivo activity of homing endonucleases. Moreover, due to its high sensitivity, it can be applied for directed evolution of homing endonucleases with altered sequence specificity.作者: arcane 時間: 2025-3-22 06:27
Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile,ingle experiment. The base preference at each position of the target sequence can be quantitated based on this assay, and the overall binding specificity of the enzyme can thereby be determined and compared with its cleavage specificity.作者: anchor 時間: 2025-3-22 10:01 作者: 北極人 時間: 2025-3-22 16:38
Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers, homing endonucleases and discuss their strengths and weaknesses in genome editing as compared to other site-specific nucleases such as zinc finger endonucleases, TALEN, and CRISPR-derived endonucleases.作者: 畢業(yè)典禮 時間: 2025-3-22 20:24 作者: 生氣地 時間: 2025-3-23 00:52
Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display, assumptions or knowledge regarding the target site. This protocol is easily integrated into the yeast surface display pipeline and is leveraged by the expansive number of existing tools for both SELEX and yeast surface display.作者: olfction 時間: 2025-3-23 01:42 作者: 不再流行 時間: 2025-3-23 05:42
Bioinformatics Identification of Coevolving Residues,e sequence alignments for coevolution inference. Furthermore, it is important to understand common signs and sources of error. When confounding factors are accounted for, coevolution is a rich resource for protein engineering information.作者: 背書 時間: 2025-3-23 12:56
Book 2014 endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of 作者: 館長 時間: 2025-3-23 15:20 作者: strdulate 時間: 2025-3-23 21:05
PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes,lternative to synthesizing artificial endonucleases. Here, we describe methods for PCR-based screening of fungal mitochondrial rRNA genes for homing endonuclease encoding sequences, and we also provide protocols for the purification and biochemical characterization of putative native homing endonucleases.作者: Carcinogen 時間: 2025-3-24 00:26
,Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE,e often expressed at very low levels making transcript mapping a challenge. Here, I present a 5′RLM-RACE protocol with special considerations for the expected abundance of homing endonucleases and for their potential to be subjected to RNA processing events.作者: 聯(lián)合 時間: 2025-3-24 03:51
Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sen. In order to delineate the target site specificity of HEs and facilitate HE engineering, we have developed a method for comprehensive target site profiling of HEs cleavage specificity using partially randomized target site libraries and high-throughput DNA sequencing.作者: Mortar 時間: 2025-3-24 10:12 作者: concentrate 時間: 2025-3-24 11:19
Taegun Kwon,Obed W. Odom,Weihua Qiu,David L. Herrinisen.Menschliche N?he, Feiern.Wie gehen wir mit Globalisierung um?.Es ist kaum zu verhindern, dass aus einer begrenzten Epidemie erneut eine globale Pandemie wird..Was haben wir gelernt?.Was k?nnen und müssen wir zukünftig besser machen?.Corona zwingt uns zu ?nderungen. Das n?chste Virus 978-3-662-63555-1978-3-662-63556-8作者: Congeal 時間: 2025-3-24 18:24 作者: 語源學 時間: 2025-3-24 19:12 作者: META 時間: 2025-3-24 23:56
Mohamed Hafez,Tuhin Kumar Guha,Chen Shen,Jyothi Sethuraman,Georg Hausnernse electromagnetic radiation are of primary and utmost importance as many of those objects are accessible only by detection of the electromagnetic radiation they emit. As such effects are typical for more remote planetary and astrophysical objects, the present chapter attempts to generalize a few o作者: Emasculate 時間: 2025-3-25 04:07 作者: Progesterone 時間: 2025-3-25 09:57
Lei Zhao,Barry L. Stoddardu besuchen. Mit dem Raucheu hier an Bord war es auch ein eigenes Ding. Unten im Speisesaal wurde Weiu, Stratzburger Bier und andere herzst?rkende Labc gegen die bekannten Tickets verabfolgt, aber — ?é prohibido fumar, expressamente prohibido fumar“ rauute mir der Steward ius Ohr, als ich zum Glase B作者: 虛情假意 時間: 2025-3-25 15:02 作者: largesse 時間: 2025-3-25 18:04 作者: Adenoma 時間: 2025-3-25 20:39
Kyle Jacoby,Andrew M. Scharenbergn schwacher und farbloser Abglanz erscheinen. Schon als Knabe hatte ich bei meiner Lieblingslektüre, den alten ?Reisebeschreibungen“, an nichts so gro?e Freude, als an den Urw?ldern Indiens und Brasiliens; als dann sp?ter Humboldts ?Ansichten der Natur“, Schleibens ?Pslanze und ihr Leben“, Kittlitz’作者: ureter 時間: 2025-3-26 01:10 作者: gerrymander 時間: 2025-3-26 05:46 作者: FAR 時間: 2025-3-26 10:00
Stefan Pellenz,Raymond J. Monnat Jr.von der Küste, die wir besucht hatten, trat ganz deutlich hervor, wie v?llig unbekannt das n?rdlich von der Station gelegene Gebiet noch war. In geologischer Beziehung konnte man deswegen auf besonders reiche Ergebnisse rechnen, in bezug auf die Geographie dieser Gegend war ich, ohne jedoch hinreich作者: accessory 時間: 2025-3-26 15:52
Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selecet sites, or many individual target sites, can be analyzed using a single transformation. Both cleavable and non-cleavable targets can be analyzed by DNA sequencing to gain information about nucleotide preference in the endonuclease’s target site.作者: Jogging 時間: 2025-3-26 17:56
A Yeast-Based Recombination Assay for Homing Endonuclease Activity,sted for their capacity to induce homologous recombination in a yeast cell. The process has been standardized and robotized so that we can generate thousands of I-CreI derivatives, characterize their cleavage profile, and deliver them for further applications in the research, therapeutic, or agrobus作者: 男生如果明白 時間: 2025-3-26 23:45 作者: 移植 時間: 2025-3-27 02:46 作者: Concomitant 時間: 2025-3-27 08:03 作者: Inoperable 時間: 2025-3-27 11:47 作者: exclamation 時間: 2025-3-27 16:53
978-1-4939-6319-5Springer Science+Business Media New York 2014作者: 加花粗鄙人 時間: 2025-3-27 19:49
Homing Endonucleases978-1-62703-968-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Musculoskeletal 時間: 2025-3-27 22:29 作者: 發(fā)炎 時間: 2025-3-28 04:42
David R. EdgellServes as a comprehensive resource to identify and characterize homing endonucleases and much more.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation作者: breadth 時間: 2025-3-28 07:34 作者: 單調性 時間: 2025-3-28 12:38
https://doi.org/10.1007/978-1-62703-968-0Binding and cleavage specificity; Genome editing; Mobile genetic elements; Recombination-based pathways作者: 高貴領導 時間: 2025-3-28 17:27 作者: Respond 時間: 2025-3-28 19:14 作者: Hypopnea 時間: 2025-3-28 23:45
Eyal Privman to terrestrial auroral phenomena, but are of a very general nature. They take place in various different objects and places in the universe when magnetized plasmas of different constitution come into contact. Hot collisionless plasmas in convective motion connected via magnetic fields to cold colli作者: 誤傳 時間: 2025-3-29 04:55 作者: 外觀 時間: 2025-3-29 07:18 作者: 會議 時間: 2025-3-29 13:16 作者: Override 時間: 2025-3-29 17:13 作者: 高談闊論 時間: 2025-3-29 19:44 作者: 是剝皮 時間: 2025-3-30 03:15 作者: 收集 時間: 2025-3-30 07:19 作者: Locale 時間: 2025-3-30 08:39
Sarah K. Baxter,Andrew M. Scharenberg,Abigail R. Lambertndischen Reisebriefen“ zu dem friedlichen und harmlosen Volk der Singhalesen. Schon seit seiner Jugendzeit hegte der berühmte Naturforscher den brennenben Wunsch, eine Tropenreise auszuführen. ?Der Wunsch, die Wunder der Tropennatur von Angesicht zu sehen, ist für jeden Naturforscher, der sich die E作者: crease 時間: 2025-3-30 15:24 作者: ironic 時間: 2025-3-30 16:59
Stefan Pellenz,Raymond J. Monnat Jr.gebende Natur mir nicht mehr neu und unbekannt war, einen ganz anderen Reiz für mich Auch bedeutete der Einsatz an harter Arbeit, deren Lohn je nach den Verh?ltnissen das gro?e Los oder nur eine Niete werden konnte, unter allen Umst?nden die herrlichste Befreiung aus der langen Einf?rmigkeit. In bez作者: 喚醒 時間: 2025-3-31 00:02
Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers,echanisms that govern the function and evolution of these genetic oddities have been well documented over the past few decades at the genetic, biochemical, and structural levels. This wealth of information has led to the manipulation and reprogramming of the endonucleases and to their exploitation i作者: malign 時間: 2025-3-31 03:06
Bioinformatic Identification of Homing Endonucleases and Their Target Sites,The public genomic sequence databases contain thousands of HEGs. This is a large and diverse arsenal of potential genome editing tools. To make use of this natural resource, one needs to identify candidate HEGs. Due to their special relationship with a host gene, it is also possible to predict their作者: ARC 時間: 2025-3-31 05:33 作者: 拉開這車床 時間: 2025-3-31 12:05 作者: 解決 時間: 2025-3-31 17:10
,Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE,uclease promoters often lack recognizable promoters making predicting the transcriptional start site challenging. Furthermore, homing endonucleases are often expressed at very low levels making transcript mapping a challenge. Here, I present a 5′RLM-RACE protocol with special considerations for the 作者: 陰謀 時間: 2025-3-31 18:59
PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydnce I-CreII is from a mobile . intron of ., it cleaves the . gene of an intronless-. strain of .. And it cleaves specifically in the . gene of ., which is naturally intronless. We have shown further that most of the repair products of an I-CreII-induced break in chloroplast DNA can be defined by PCR作者: refraction 時間: 2025-3-31 22:22 作者: 羅盤 時間: 2025-4-1 04:24 作者: Nuance 時間: 2025-4-1 10:05
A Yeast-Based Recombination Assay for Homing Endonuclease Activity,ite of the break. Such mechanisms can thus be used for all the applications covered today by the generic name of “genome engineering”: targeted sequence insertion, removal, or editing. However, before being able to address those applications, the engineering of HEs must be mastered so that any poten
