標題: Titlebook: Glioblastoma; Methods and Protocol Dimitris G.‘Placantonakis Book 2018 Springer Science+Business Media, LLC 2018 xenografts.GBM cancer stem [打印本頁] 作者: BROOD 時間: 2025-3-21 17:35
書目名稱Glioblastoma影響因子(影響力)
書目名稱Glioblastoma影響因子(影響力)學科排名
書目名稱Glioblastoma網絡公開度
書目名稱Glioblastoma網絡公開度學科排名
書目名稱Glioblastoma被引頻次
書目名稱Glioblastoma被引頻次學科排名
書目名稱Glioblastoma年度引用
書目名稱Glioblastoma年度引用學科排名
書目名稱Glioblastoma讀者反饋
書目名稱Glioblastoma讀者反饋學科排名
作者: 聲音刺耳 時間: 2025-3-21 22:26 作者: follicular-unit 時間: 2025-3-22 01:42 作者: Crayon 時間: 2025-3-22 04:44 作者: 聯想 時間: 2025-3-22 10:00 作者: largesse 時間: 2025-3-22 16:20 作者: largesse 時間: 2025-3-22 19:01 作者: 中世紀 時間: 2025-3-22 22:54
https://doi.org/10.1007/978-1-4939-7659-1xenografts; GBM cancer stem cells; embryonic stem cells; neuro-oncology; tumor作者: ARCHE 時間: 2025-3-23 01:43 作者: BRACE 時間: 2025-3-23 09:22 作者: Figate 時間: 2025-3-23 12:25
Hong Kong Architecture 1945-2015issection (LCM) offers a unique opportunity to study cells in their topological contexts. This chapter focuses on the preparation of LCM membrane slides, tissue staining and laser microdissection of cells of interest from frozen or formalin-fixed, paraffin-embedded glioblastoma tissue.作者: 鴿子 時間: 2025-3-23 14:54
Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF,ype. The variability in blood perfusion and oxygen tension within tumors suggests that ambient pH values fluctuate across different tumor territories. This chapter describes a detailed protocol for measuring intracellular pH in patient-derived GBM cells in vitro, using the fluorescent pH sensitive dye BCECF.作者: Override 時間: 2025-3-23 21:52 作者: obsession 時間: 2025-3-23 23:54 作者: Yourself 時間: 2025-3-24 04:44
978-1-4939-8536-4Springer Science+Business Media, LLC 2018作者: chronicle 時間: 2025-3-24 10:33
Glioblastoma978-1-4939-7659-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: AVANT 時間: 2025-3-24 13:15
Hom?opathie - die Fakten [unverdünnt]ent of disease. The goal is to use high-throughput sequencing to identify specific variants that drive tumorigenesis within each individual’s tumor genomic profile. The significance of copy number and structural variants in glioblastoma makes it essential to broaden the search beyond oncogenic singl作者: ear-canal 時間: 2025-3-24 17:15
https://doi.org/10.1007/978-3-662-55549-1 glioblastoma molecular profiles. The procedure spans four days, and can be performed by a single laboratory technician. Starting with as little as 250 ng of DNA input, this method allows the flexibility to begin with DNA derived from either formalin-fixed, paraffin-embedded (FFPE) or fresh tissue a作者: 鄙視讀作 時間: 2025-3-24 21:04
https://doi.org/10.1007/978-3-642-77601-4 or by tumorsphere culture in suspension. Here, we provide a detailed protocol for establishing patient-derived tumorsphere cultures. Such cultures are enriched for GBM stem cells (GSCs) and can be used to generate orthotopic tumor xenografts in the brain of immunocompromised mice. We also point out作者: 蔑視 時間: 2025-3-24 23:49
Honda‘s Global Local Corporationcell sorting (FACS) using CD133 as a surface marker. The use of a directly conjugated antibody to an APC fluorophore against the CD133 molecule provides sufficient and clear detection of positive cells from the rest of the population. This strategy avoids an unnecessary secondary antibody incubation作者: conscribe 時間: 2025-3-25 03:31 作者: 符合規(guī)定 時間: 2025-3-25 09:44
Honey in Food Science and Physiology or progression and recurrence, by virtue of their robust tumor-propagating potential and resistance to conventional chemoradiotherapy. Therefore, targeting GSCs holds significant therapeutic promise. Expression of CD133 (PROM1), a cell surface glycoprotein, has been associated with the GSC phenotype作者: Biomarker 時間: 2025-3-25 14:43
H.R. Hepburn,C.W.W. Pirk,O. Duangphakdeeype. The variability in blood perfusion and oxygen tension within tumors suggests that ambient pH values fluctuate across different tumor territories. This chapter describes a detailed protocol for measuring intracellular pH in patient-derived GBM cells in vitro, using the fluorescent pH sensitive d作者: 現存 時間: 2025-3-25 16:54
Honeybee Neurobiology and Behaviorcussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.作者: 流出 時間: 2025-3-25 21:25 作者: 窗簾等 時間: 2025-3-26 02:13
Swarming, Migration and Absconding,al approaches have been described toward quantitative proteomics. In this chapter, we focus on Tandem Mass Tag (TMT) isobaric labeling of peptides for global, relative quantitation of proteins and phosphopeptides. To date, there has been no published protocol describing chemical labeling of small am作者: 吃掉 時間: 2025-3-26 06:45 作者: 輕推 時間: 2025-3-26 09:56 作者: 指令 時間: 2025-3-26 16:22 作者: 玉米 時間: 2025-3-26 19:19
https://doi.org/10.1007/978-1-349-08784-6noninvasive imaging. This chapter describes in detail how to assess xenograft size and growth using bioluminescent imaging with IVIS (in vivo imaging system). This form of imaging (a) can be performed without the help of a trained technician, (b) is a very quick procedure, allowing simultaneous imag作者: Pituitary-Gland 時間: 2025-3-26 23:51 作者: 有幫助 時間: 2025-3-27 01:15 作者: 無思維能力 時間: 2025-3-27 06:47 作者: 昏睡中 時間: 2025-3-27 11:49 作者: 牽索 時間: 2025-3-27 15:48
Whole Genome Sequencing-Based Discovery of Structural Variants in Glioblastoma,ent of disease. The goal is to use high-throughput sequencing to identify specific variants that drive tumorigenesis within each individual’s tumor genomic profile. The significance of copy number and structural variants in glioblastoma makes it essential to broaden the search beyond oncogenic singl作者: CREST 時間: 2025-3-27 18:03
Whole Genome DNA Methylation Analysis of Human Glioblastoma Using Illumina BeadArrays, glioblastoma molecular profiles. The procedure spans four days, and can be performed by a single laboratory technician. Starting with as little as 250 ng of DNA input, this method allows the flexibility to begin with DNA derived from either formalin-fixed, paraffin-embedded (FFPE) or fresh tissue a作者: 真實的你 時間: 2025-3-27 22:28
Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens, or by tumorsphere culture in suspension. Here, we provide a detailed protocol for establishing patient-derived tumorsphere cultures. Such cultures are enriched for GBM stem cells (GSCs) and can be used to generate orthotopic tumor xenografts in the brain of immunocompromised mice. We also point out作者: Cosmopolitan 時間: 2025-3-28 03:11
Isolation of Glioblastoma Stem Cells with Flow Cytometry,cell sorting (FACS) using CD133 as a surface marker. The use of a directly conjugated antibody to an APC fluorophore against the CD133 molecule provides sufficient and clear detection of positive cells from the rest of the population. This strategy avoids an unnecessary secondary antibody incubation作者: 幸福愉悅感 時間: 2025-3-28 09:37
Lentiviral Transduction of Primary Human Glioblastoma Cultures, cultures. Lentiviruses stably transduce both dividing and non-dividing cells, such as quiescent cancer stem cell populations. The viral envelope is pseudotyped with the vesicular stomatitis virus envelope glycoprotein G (VSV-G), which renders the lentiviral particles pantropic, so that they can inf作者: Admonish 時間: 2025-3-28 10:30
Selective Targeting of CD133-Expressing Glioblastoma Stem Cells Using Lentiviral Vectors,or progression and recurrence, by virtue of their robust tumor-propagating potential and resistance to conventional chemoradiotherapy. Therefore, targeting GSCs holds significant therapeutic promise. Expression of CD133 (PROM1), a cell surface glycoprotein, has been associated with the GSC phenotype作者: ineffectual 時間: 2025-3-28 14:45
Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF,ype. The variability in blood perfusion and oxygen tension within tumors suggests that ambient pH values fluctuate across different tumor territories. This chapter describes a detailed protocol for measuring intracellular pH in patient-derived GBM cells in vitro, using the fluorescent pH sensitive d作者: 光明正大 時間: 2025-3-28 21:49
Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro,cussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.作者: 不自然 時間: 2025-3-29 02:31 作者: NEXUS 時間: 2025-3-29 06:45 作者: 規(guī)章 時間: 2025-3-29 09:41
Single-Cell RNA Sequencing of Glioblastoma Cells,ional RNASeq in which entire populations are sequenced in bulk, sc-RNASeq can be beneficial when trying to better understand gene expression patterns in markedly heterogeneous populations of cells or when trying to identify transcriptional signatures of rare cells that may be underrepresented when u作者: 傳染 時間: 2025-3-29 15:02 作者: ELUDE 時間: 2025-3-29 15:52
Orthotopic Patient-Derived Glioblastoma Xenografts in Mice,se brain are obtained by injection of GBM stem-like cells derived from fresh surgical specimens. These xenografts reproduce GBM’s histologic complexity and hallmark biological behaviors, such as brain invasion, angiogenesis, and resistance to therapy. This method has become essential for analyzing m作者: 使饑餓 時間: 2025-3-29 23:17
Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice,noninvasive imaging. This chapter describes in detail how to assess xenograft size and growth using bioluminescent imaging with IVIS (in vivo imaging system). This form of imaging (a) can be performed without the help of a trained technician, (b) is a very quick procedure, allowing simultaneous imag作者: 詳細目錄 時間: 2025-3-30 03:23 作者: Asymptomatic 時間: 2025-3-30 04:12 作者: conformity 時間: 2025-3-30 12:14
Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma,protocol is unique from many others in that the use of a selective lymphocyte isolation procedure, such as a Ficoll or Percoll gradient, is not used. We find that staining of TILs and analysis by flow cytometry is not affected by the presence of heterogeneous populations, while other selective isola作者: 組裝 時間: 2025-3-30 15:43
Modeling Glioma with Human Embryonic Stem Cell-Derived Neural Lineages,es mutations leading to oncogenic transformation. Thanks to advances in human stem cell biology, it has become possible to derive human cell types that represent putative cells-of-origin in vitro and model the gliomagenesis process by systematically introducing genetic alterations in these human cel作者: Cardiac-Output 時間: 2025-3-30 18:41
1064-3745 ation advice from the experts.This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures. Chapters focus on subpopulations of cells with stem-like properties, laser capture micro作者: cardiopulmonary 時間: 2025-3-31 00:25
https://doi.org/10.1007/978-3-662-55549-10 ng of DNA input, this method allows the flexibility to begin with DNA derived from either formalin-fixed, paraffin-embedded (FFPE) or fresh tissue and is compatible with an Illumina iScan or HiScan system.作者: hemoglobin 時間: 2025-3-31 04:51 作者: Bumble 時間: 2025-3-31 06:11
Swarming, Migration and Absconding, global, relative quantitation of proteins and phosphopeptides. To date, there has been no published protocol describing chemical labeling of small amounts of peptides specifically extracted from small tumor samples, for which rigorous sample preparation is necessary to ensure reproducible TMT labeling.作者: TOXIC 時間: 2025-3-31 11:43
https://doi.org/10.1057/9780230245433We find that staining of TILs and analysis by flow cytometry is not affected by the presence of heterogeneous populations, while other selective isolation procedures can significantly decrease lymphocyte yield from already rare populations.作者: UNT 時間: 2025-3-31 16:22 作者: 燈泡 時間: 2025-3-31 19:29
https://doi.org/10.1007/978-3-642-71458-0ification and differential analysis are performed using commercial or open source software. Protocols outlined in this chapter describe how nano-LC-MS can be applied to investigate metabolic profiling with limited biomass amount.作者: Coronary-Spasm 時間: 2025-4-1 00:27
Honeybees in Natural Ecosystems,s using the C1 Fluidigm system. The protocol details the use of the C1 integrated fluidics circuit (IFC) for capturing, imaging and lysing cells; performing reverse transcription; and generating cDNA libraries that are ready for sequencing and analysis.作者: Charade 時間: 2025-4-1 05:05 作者: 序曲 時間: 2025-4-1 07:41 作者: 厭煩 時間: 2025-4-1 11:25
