標(biāo)題: Titlebook: Genotyping; Methods and Protocol Stefan J. White,Stuart Cantsilieris Book 2017 Springer Science+Business Media New York 2017 DNA.Taqman-bas [打印本頁] 作者: 調(diào)停 時間: 2025-3-21 19:10
書目名稱Genotyping影響因子(影響力)
書目名稱Genotyping影響因子(影響力)學(xué)科排名
書目名稱Genotyping網(wǎng)絡(luò)公開度
書目名稱Genotyping網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Genotyping被引頻次
書目名稱Genotyping被引頻次學(xué)科排名
書目名稱Genotyping年度引用
書目名稱Genotyping年度引用學(xué)科排名
書目名稱Genotyping讀者反饋
書目名稱Genotyping讀者反饋學(xué)科排名
作者: 基因組 時間: 2025-3-21 23:29
Genotyping DNA Variants with High-Resolution Melting Analysis,destructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.作者: 誘使 時間: 2025-3-22 04:20 作者: arsenal 時間: 2025-3-22 08:06
In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification,ys rely on whole specimen extracts, where heterogeneous spatial context of the specimen is lost. This chapter describes an up-to-date protocol for multiplexed, in situ genotyping of RNA in preserved tissue and cell lines, using padlock probes and rolling circle amplification. The presented approach 作者: 處理 時間: 2025-3-22 10:50
,The MassARRAY? System for Targeted SNP Genotyping, The use of genome-wide SNP genotyping arrays has become increasingly more commonplace for gene discovery. However, smaller-scale genotyping platforms capable of efficiently genotyping tens to hundreds of SNPs are still crucial for many aspects of this work, including replication of associations. Th作者: packet 時間: 2025-3-22 15:02
Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs),cing of genomic DNA. Several thousand genomic targets can be selectively captured using long oligonucleotides containing unique targeting arms and universal linkers. The ability to append sequencing adaptors and sample-specific barcodes allows large-scale pooling and subsequent high-throughput seque作者: packet 時間: 2025-3-22 18:06
Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnos4. In unaffected individuals the number of 3.3 kb D4Z4 units varies between 8 and 100, whereas 1–10 units are seen in FSHD1 cases. A homologous and heterogenous D4Z4 array can be found on chromosome 10q, but contractions of this array are typically not associated with FSHD. Discriminating between th作者: 油氈 時間: 2025-3-23 00:56
Analysis of Copy Number Variation Using the Paralogue Ratio Test (PRT),ource of genetic variation within species. However, reliably determining copy number of a particular DNA sequence for a large number of samples can be challenging. Here, I describe and review the paralogue ratio test (PRT) in detail. PRT was developed to robustly type the CNV of the beta-defensin lo作者: 很像弓] 時間: 2025-3-23 02:13
Genotyping Multiallelic Copy Number Variation with Multiplex Ligation-Dependent Probe Amplificationers of specific genes have been associated with different diseases. Precise genotyping of these loci can be complicated, and relies on accurate assays. Multiplex ligation-dependent probe amplification (MLPA) is a PCR-based approach that allows copy number determination of up to 50 genomic loci in a 作者: consolidate 時間: 2025-3-23 08:22
Analysis of Multiallelic CNVs by Emulsion Haplotype Fusion PCR,s very numerous small reaction chambers in which different PCR products from a single genomic DNA molecule are condensed into short joint products, to unite sequences in . from widely separated genomic sites. These products can therefore provide information about the arrangement of sequences and var作者: Hallowed 時間: 2025-3-23 11:23 作者: 反饋 時間: 2025-3-23 15:16 作者: 使增至最大 時間: 2025-3-23 18:36
Targeted Locus Amplification and Next-Generation Sequencing,und genes of interest and in an allele-specific manner remains a challenge. Targeted locus amplification (TLA) is a cross-linking-based technique that generates complex DNA libraries covering >100 kb of contiguous sequence surrounding one primer pair complementary to a short locus-specific sequence.作者: 披肩 時間: 2025-3-24 01:09
Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, andc clades, molecular groups, or subpopulations of a species, as well as individual strains or clones. However, conventional MLST is costly and time consuming, which limits its power for genotyping large numbers of samples. Here, we describe a new MLST method that uses next-generation sequencing, a mu作者: 付出 時間: 2025-3-24 05:01
Rapid SNP Detection and Genotyping of Bacterial Pathogens by Pyrosequencing,ne outbreaks and bioterrorist events. Whole genome sequencing (WGS) is leading the way in terms of expanding our ability to identify and characterize bacteria through the identification of subtle differences between genomes (e.g. single nucleotide polymorphisms (SNPs) and insertions/deletions). Mode作者: MAG 時間: 2025-3-24 09:04
Methods for Genotyping-by-Sequencing,ns a powerful approach for discovering the causal genes underlying phenotypes. For genetic mapping, the process of genotyping was previously a major rate-limiting step. Modern sequencing technology has greatly improved the resolution and speed of genetic mapping by reducing the time, labor, and cost作者: Classify 時間: 2025-3-24 12:04 作者: 注入 時間: 2025-3-24 16:55 作者: myocardium 時間: 2025-3-24 22:21
https://doi.org/10.1007/978-3-662-29087-3ort tandem repeat (STR) systems in a multiplex reaction with simultaneous detection helps to obtain more information from a DNA sample where its availability may be limited. Here, we introduce and describe this commonly used genotyping technique, providing an overview on available resources on STRs, multiplex design, and analysis.作者: Bombast 時間: 2025-3-25 02:49 作者: 清醒 時間: 2025-3-25 05:36 作者: Interdict 時間: 2025-3-25 10:34 作者: Stress 時間: 2025-3-25 15:11 作者: 厭食癥 時間: 2025-3-25 18:29
Targeted Locus Amplification and Next-Generation Sequencing, In combination with next-generation sequencing, TLA enables the complete sequencing and haplotyping of targeted regions of interest. Here we outline the basis of TLA, together with a detailed protocol of the technique.作者: hallow 時間: 2025-3-25 20:40
,The MassARRAY? System for Targeted SNP Genotyping, capable of efficiently genotyping tens to hundreds of SNPs are still crucial for many aspects of this work, including replication of associations. The Agena Bioscience MassARRAY System is one such platform. Here, we provide a guide to using the MassARRAY System, from assay design, through mass spectrometry, to generation of genotype data.作者: 熱烈的歡迎 時間: 2025-3-26 02:07 作者: 壁畫 時間: 2025-3-26 07:34
Genotyping Multiallelic Copy Number Variation with Multiplex Ligation-Dependent Probe Amplification. Multiplex ligation-dependent probe amplification (MLPA) is a PCR-based approach that allows copy number determination of up to 50 genomic loci in a single reaction. In this chapter, we outline the basic protocol, with a particular emphasis on the appropriate approach to accurately genotype multiallelic copy numbers.作者: 大氣層 時間: 2025-3-26 11:19
Quantitative DNA Analysis Using Droplet Digital PCR,ts allows the precise quantification of a given sequence. In this chapter we briefly outline the basis of ddPCR, and describe two different applications using the Bio-Rad QX200 system: genotyping copy number variation and quantification of Illumina sequencing libraries.作者: 火花 時間: 2025-3-26 12:46
Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII,In this setup we first generate long-range PCR products for two partially overlapping 7.7 and 9.2 kb MT DNA-specific amplicons, add sample-specific barcodes, and sequence these on the PacBio RSII system to obtain full-length MT DNA sequences for genotyping/haplotyping purposes.作者: Left-Atrium 時間: 2025-3-26 17:11 作者: 郊外 時間: 2025-3-26 21:00
Die Berufssituation der Sozialarbeitere experimental procedure for typing hundreds of samples, and finally the data evaluation. Our goal is to provide a guideline for developing genotyping assays using these two approaches that render reliable and reproducible genotype calls involving minimal optimization.作者: 猛擊 時間: 2025-3-27 02:29
https://doi.org/10.1007/978-3-322-88566-1to their targets. Following this conversion, padlock probes are copied in situ by rolling circle amplification and labeled with flourophore-conjugated probes, allowing for their detection with conventional fluorescence microscopy.作者: Wordlist 時間: 2025-3-27 06:59 作者: 故意釣到白楊 時間: 2025-3-27 12:00
In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification,to their targets. Following this conversion, padlock probes are copied in situ by rolling circle amplification and labeled with flourophore-conjugated probes, allowing for their detection with conventional fluorescence microscopy.作者: COLIC 時間: 2025-3-27 15:02
Die Beschr?nkung des Betriebsausgabenabzugs designing successful PRT assays using both manual and bioinformatics methods, how to optimize experimental conditions, and approaches for analyzing the data. I discuss strengths and weaknesses of the approach, and how to troubleshoot results, as well as the range of problems to which PRT can be a potential solution.作者: 墻壁 時間: 2025-3-27 20:09
Zusammenfassung der Ergebnisse, typing of inversions, and determining the configuration of sequence variants in multiallelic CNVs. In this description we outline the rationale for the application of emulsion-fusion PCR methods to the analysis of multiallelic CNVs, and give practical details for our own implementation of the method in that context.作者: habile 時間: 2025-3-28 01:06 作者: Bereavement 時間: 2025-3-28 02:45 作者: mosque 時間: 2025-3-28 08:25 作者: 小教堂 時間: 2025-3-28 12:58
https://doi.org/10.1007/978-3-662-29086-6 capable of efficiently genotyping tens to hundreds of SNPs are still crucial for many aspects of this work, including replication of associations. The Agena Bioscience MassARRAY System is one such platform. Here, we provide a guide to using the MassARRAY System, from assay design, through mass spectrometry, to generation of genotype data.作者: 揉雜 時間: 2025-3-28 15:48
https://doi.org/10.1007/978-3-663-06796-2terogenous D4Z4 array can be found on chromosome 10q, but contractions of this array are typically not associated with FSHD. Discriminating between the chromosome 4 and chromosome 10 D4Z4 arrays, as well as determining the array size, requires the use of pulsed-field gel electrophoresis, Southern blotting, and the isolation of high-quality DNA.作者: uncertain 時間: 2025-3-28 22:19
https://doi.org/10.1007/978-3-531-90974-5. Multiplex ligation-dependent probe amplification (MLPA) is a PCR-based approach that allows copy number determination of up to 50 genomic loci in a single reaction. In this chapter, we outline the basic protocol, with a particular emphasis on the appropriate approach to accurately genotype multiallelic copy numbers.作者: parasite 時間: 2025-3-29 00:35
https://doi.org/10.1007/978-3-662-34625-9ts allows the precise quantification of a given sequence. In this chapter we briefly outline the basis of ddPCR, and describe two different applications using the Bio-Rad QX200 system: genotyping copy number variation and quantification of Illumina sequencing libraries.作者: PALL 時間: 2025-3-29 03:33 作者: Rinne-Test 時間: 2025-3-29 09:18 作者: chassis 時間: 2025-3-29 12:08
Auf die richtigen Mitarbeiter kommt es an,t targets canonical single nucleotide polymorphisms (canSNPs) of evolutionary importance in ., the causative agent of Anthrax. The second assay detects Shiga-toxin (.) genes, which are associated with virulence in . and ., and differentiates the subtypes of .-1 and .-2 based on SNP loci. These rapid作者: 愚笨 時間: 2025-3-29 17:01 作者: Conspiracy 時間: 2025-3-29 22:49
https://doi.org/10.1007/978-3-322-81520-0etion, duplication, insertion, inversion, and conversion. The basics of how to apply the recommendations to describe sequence variants will be explained here. An extensive description of the current HGVS guidelines (version 15.11) is available online at ..作者: Catheter 時間: 2025-3-30 02:53 作者: Obligatory 時間: 2025-3-30 07:42 作者: Proponent 時間: 2025-3-30 08:57 作者: Commentary 時間: 2025-3-30 12:37 作者: saphenous-vein 時間: 2025-3-30 19:06 作者: 彎腰 時間: 2025-3-30 21:04 作者: nocturia 時間: 2025-3-31 03:14 作者: 歡樂東方 時間: 2025-3-31 07:05
Die Berufserziehung in der Landwirtschaftdestructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.作者: oracle 時間: 2025-3-31 13:08
Genotyping DNA Variants with High-Resolution Melting Analysis,destructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.作者: 聾子 時間: 2025-3-31 16:12 作者: 喊叫 時間: 2025-3-31 20:08 作者: Mirage 時間: 2025-3-31 22:31 作者: 水槽 時間: 2025-4-1 04:13
Die Berufserziehung in der Landwirtschaftdestructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.作者: condescend 時間: 2025-4-1 06:12 作者: 英寸 時間: 2025-4-1 13:09
