標(biāo)題: Titlebook: Genetic Library Construction and Screening; Advanced Techniques R. Curtis Bird,Bruce F. Smith Book 2002 Springer-Verlag Berlin Heidelberg [打印本頁(yè)] 作者: estrange 時(shí)間: 2025-3-21 18:42
書(shū)目名稱(chēng)Genetic Library Construction and Screening影響因子(影響力)
書(shū)目名稱(chēng)Genetic Library Construction and Screening影響因子(影響力)學(xué)科排名
書(shū)目名稱(chēng)Genetic Library Construction and Screening網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱(chēng)Genetic Library Construction and Screening網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱(chēng)Genetic Library Construction and Screening被引頻次
書(shū)目名稱(chēng)Genetic Library Construction and Screening被引頻次學(xué)科排名
書(shū)目名稱(chēng)Genetic Library Construction and Screening年度引用
書(shū)目名稱(chēng)Genetic Library Construction and Screening年度引用學(xué)科排名
書(shū)目名稱(chēng)Genetic Library Construction and Screening讀者反饋
書(shū)目名稱(chēng)Genetic Library Construction and Screening讀者反饋學(xué)科排名
作者: 食草 時(shí)間: 2025-3-21 22:10 作者: 規(guī)章 時(shí)間: 2025-3-22 01:36 作者: 左右連貫 時(shí)間: 2025-3-22 05:16 作者: 威脅你 時(shí)間: 2025-3-22 12:28 作者: Petechiae 時(shí)間: 2025-3-22 14:08 作者: Petechiae 時(shí)間: 2025-3-22 18:36
Differential Display to Identify Steroid-Induced Genes in Endocrine Cells likelihood of success comparing different methods, the steadily increasing number of citations using differential display for gene identification supports the notion that the method is effective. Even with these successes, differential display is not without pitfalls and presents challenges at seve作者: 絕食 時(shí)間: 2025-3-23 00:16
Yeast One and Two Hybrid cDNA Cloning used by biologists to clone new genes based on protein-protein interactions, including co-immunoprecipitation, affinity purification, and the yeast two-hybrid system. The yeast two-hybrid cDNA cloning technology is a powerful in vivo protein-protein interaction assay first introduced in 1989 by Fie作者: 向外供接觸 時(shí)間: 2025-3-23 05:16 作者: 燒烤 時(shí)間: 2025-3-23 07:37 作者: AMOR 時(shí)間: 2025-3-23 11:17
Brentanos Versuch der Grundlegung der Ethik,ssion, modification or further subcloning. A wide variety of strategies have been employed and are available in commercially supplied kits (see Page 30 for a listing of manufacturers of PCR cloning kits or vectors). These approaches include simple but relatively inefficient blunt ligation schemes th作者: 借喻 時(shí)間: 2025-3-23 17:48
Das Problem der Industriearbeiting numerous living organisms or cells for naturally existing mutants, often following exposure to a mutagen. Since DNA is reproduced in vivo quite faithfully according to its template and most errors are efficiently detected and reversed by DNA repair systems, naturally occurring mutations are rare作者: FANG 時(shí)間: 2025-3-23 21:17
https://doi.org/10.1007/978-3-322-98641-2nces for target identification, sequence determination, subsequent use as molecular probes, or as functional units which can be expressed in appropriate vector systems. This chapter describes the technique of Long RT-PCR followed by cloning of the PCR products into plasmid vectors. Essentially, this作者: 爵士樂(lè) 時(shí)間: 2025-3-23 22:41 作者: 狂亂 時(shí)間: 2025-3-24 04:46
https://doi.org/10.1007/978-3-642-90660-2on, the transcripts that are newly transcribed as a result of altered physiology. Whether in response to a natural phenomenon such as a growth factor or morphogen stimulation, or in response to environmental stimulation or stress, new transcripts appear as a result of newly stimulated gene expressio作者: 十字架 時(shí)間: 2025-3-24 06:33 作者: intellect 時(shí)間: 2025-3-24 12:14 作者: 愛(ài)了嗎 時(shí)間: 2025-3-24 18:07 作者: 清醒 時(shí)間: 2025-3-24 19:55
978-3-642-47733-1Springer-Verlag Berlin Heidelberg 2002作者: 不適 時(shí)間: 2025-3-25 02:05 作者: Palpate 時(shí)間: 2025-3-25 03:57
R. Curtis Bird,Bruce F. SmithA comprehensive, up-to-date guide on all aspects of synthesis and screening of genetic libraries.Detailed step-by-step protocols and extensive hands-on advice gleaned from the authors‘ experience.Perf作者: assail 時(shí)間: 2025-3-25 10:00
Das Problem der theoretischen TermeThe study of molecular interactions is becoming more and more important in all fields of biomedical and biological research. During the past decade, independent systems for large scale studying of such interactions have been developed and made commercially available.作者: laparoscopy 時(shí)間: 2025-3-25 12:14 作者: Cpap155 時(shí)間: 2025-3-25 18:43
Two Hybrid cDNA CloningThe study of molecular interactions is becoming more and more important in all fields of biomedical and biological research. During the past decade, independent systems for large scale studying of such interactions have been developed and made commercially available.作者: 蒸發(fā) 時(shí)間: 2025-3-25 20:07 作者: Cuisine 時(shí)間: 2025-3-26 01:03
https://doi.org/10.1007/978-3-642-56408-62-Hybrid; DNA; Gene Cloning; Gene Library; Molecular Biology; PCR; Polymerase Chain Reaction; RNA; cDNA; clon作者: gerontocracy 時(shí)間: 2025-3-26 08:07 作者: 匍匐 時(shí)間: 2025-3-26 09:20 作者: 我邪惡 時(shí)間: 2025-3-26 14:30
Die Stellengliederung des Produkt-Managementnal detection on high density filters that allows us to routinely screen about 50 probes per day on membranes each carrying 55,000 individual clones. This fast and cost-effective alternative to massive parallel PCR protocols requires little specialized equipment and can be operational within a few days.作者: 繁重 時(shí)間: 2025-3-26 20:20
High-Throughput Library Screening by Fluorescent Hybridizations on Gridded Membranesnal detection on high density filters that allows us to routinely screen about 50 probes per day on membranes each carrying 55,000 individual clones. This fast and cost-effective alternative to massive parallel PCR protocols requires little specialized equipment and can be operational within a few days.作者: ARCH 時(shí)間: 2025-3-27 00:59 作者: commute 時(shí)間: 2025-3-27 04:20 作者: maculated 時(shí)間: 2025-3-27 09:15 作者: 啞巴 時(shí)間: 2025-3-27 11:39
Strategies for cDNA Cloning and Mapping RNA Transcripts as determining any splicing (including alternative splicing) patterns can lead to erroneous conclusions. Investigations such as the identification of promoters or enhancers also require that the primary structure of the transcript be previously determined.作者: oxidant 時(shí)間: 2025-3-27 16:23
Site Directed Deletion, Insertion, and Substitution using PCR significant improvements have been made in using thermolabile polymerases as enzymes and double-stranded DNA as templates, by integrating strong selection methods, e.g., Unique Site Elimination based on restriction digestion at a site unique to the wild type (.), into the mutagenesis methods.作者: handle 時(shí)間: 2025-3-27 21:21
Yeast One and Two Hybrid cDNA Cloningught to the promoter containing the GAL4 DNA binding sites and will activate the transcription of reporter gene HIS3 or lacZ. The two-hybrid system can be used to clone cDNA encoding a novel protein that interacts with a known protein (bait) in yeast. It can also be used to study protein-protein interactions between two known proteins.作者: 托運(yùn) 時(shí)間: 2025-3-28 02:01 作者: modish 時(shí)間: 2025-3-28 05:04
Long RT-PCR Cloning — Amplification of Full-Length Enterovirus Genomesnd Taq polymerases such as rTth DNA polymerase, XL (PE Applied Biosystems), Deep Vent DNA polymerase (New England Biolabs) and the polymerase mix provided with the Advantage 2 PCR Enzyme System (BD Biosciences, Clonetech, UK) which have high fidelity rates and can generate much longer PCR products. 作者: 相同 時(shí)間: 2025-3-28 07:24
Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzedategies have been developed to add a determined sequence (anchor) at the 3′ end of the first-strand cDNA. These strategies include: (1) oligo (dG) or oligo (dA) tailing by terminal deoxynu- cleotidyltransferase (.; .); ( 2) the use of T4 RNA ligase to covalently attach a single-stranded (ss) anchor 作者: 記憶 時(shí)間: 2025-3-28 11:19 作者: 向宇宙 時(shí)間: 2025-3-28 15:21
Brentanos Versuch der Grundlegung der Ethik,he reaction to proceed in the direction of ligation of the insert, as the ligated product is not digestible by . (.). Other recent innovations include the addition of 5′ AATTC tails to PCR primers followed by amplification in a mix containing phosphorothioate dGTP. The PCR product is then digested w作者: 誹謗 時(shí)間: 2025-3-28 19:10 作者: BACLE 時(shí)間: 2025-3-29 00:56
https://doi.org/10.1007/978-3-663-16173-8ategies have been developed to add a determined sequence (anchor) at the 3′ end of the first-strand cDNA. These strategies include: (1) oligo (dG) or oligo (dA) tailing by terminal deoxynu- cleotidyltransferase (.; .); ( 2) the use of T4 RNA ligase to covalently attach a single-stranded (ss) anchor 作者: 狂怒 時(shí)間: 2025-3-29 03:17
https://doi.org/10.1007/978-3-642-90660-2ining a bias-free sample of unique transcripts in sufficient quantities to allow efficient recombinant library construction. Most techniques are anything but bias-free and so will result in the recovery of differing, though frequently overlapping, subsets of transcripts. Application of the appropria作者: FLIP 時(shí)間: 2025-3-29 10:12
Pieter Glasbergen,Henri J. M. Goverdeite is even considered to be a development site or not — if there is no market for a superstore in a small town, no site within the town can be regarded as a development site for this purpose. Secondly, it has an obvious bearing on the value of the site. Assume two pieces of land on the edge of a to作者: 過(guò)濾 時(shí)間: 2025-3-29 15:20
Lucky Asuelime,Suzanne Franciswo decades. Through a steady stream of experimental research, tool-building efforts, and theoretical studies, the design of an instruction-set architecture, once considered an art, has been transformed into one of the most quantitative branches of computer technology. At the same time, better unders作者: Mosaic 時(shí)間: 2025-3-29 18:45
https://doi.org/10.1007/978-3-322-85818-4the end of geography and the rise of conflicts and clashes both actual and potential between developed and developing countries. Its effects however, are often more misunderstood than understood. The output of literature in this new area in terms of theories, paradigms and opinionated discussion is
