標題: Titlebook: Gene Transfer and Expression; A Laboratory Manual Michael Kriegler Book 1990 Stockton Press 1990 Calcium.DNA.nucleic acid.protein.RNA [打印本頁] 作者: Intermediary 時間: 2025-3-21 19:11
書目名稱Gene Transfer and Expression影響因子(影響力)
書目名稱Gene Transfer and Expression影響因子(影響力)學(xué)科排名
書目名稱Gene Transfer and Expression網(wǎng)絡(luò)公開度
書目名稱Gene Transfer and Expression網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Gene Transfer and Expression被引頻次
書目名稱Gene Transfer and Expression被引頻次學(xué)科排名
書目名稱Gene Transfer and Expression年度引用
書目名稱Gene Transfer and Expression年度引用學(xué)科排名
書目名稱Gene Transfer and Expression讀者反饋
書目名稱Gene Transfer and Expression讀者反饋學(xué)科排名
作者: Irrepressible 時間: 2025-3-21 21:17
Vectorsrengths and weaknesses, many of which are presented in abbreviated form in Table 2-1. In my review of these creations, I will illustrate how they can be used, as well as provide sufficient information to allow you to incorporate your own gene or cDNA inserts into these vectors. Before I describe thi作者: Default 時間: 2025-3-22 01:22
Processing of Proteins Encoded by Transferred Genes study the gene product of interest. If that gene product is a protein molecule, you have a substantial armamentarium of methods at your disposal. In this section, I briefly describe (1) factors that can be experimentally manipulated to enhance mRNA translation, (2) the mechanism of protein processi作者: 完成 時間: 2025-3-22 06:15 作者: 熱烈的歡迎 時間: 2025-3-22 09:02
DNA Transfer (2) create DNA-containing complexes whose charge characteristics are compatible with DNA uptake by the target cell; or (3) result in the transient formation of pores in the plasma membrane of a cell exposed to an electric pulse, pores of sufficient size to allow DNA to enter the target cell.作者: frenzy 時間: 2025-3-22 13:08
Selection and Amplificationtion can be infected with a recombinant retrovirus. All other means of stable transformation are less efficient. To facilitate the isolation of cells stably transformed with the DNA of interest, a gene encoding a dominant selectable marker is usually included in the transfection protocol. Such a gen作者: frenzy 時間: 2025-3-22 19:05
Expression Cloningis type include selectable markers and oncogenes, as well as cytokines (Wong et al., 1985) and membrane proteins including cell-surface antigens (Aruffo and Seed, 1987) and cellular receptors (D’Andrea et al., 1989).作者: Glaci冰 時間: 2025-3-22 23:32
PCR-Based Expression chosen because it involves many of the types of problems one can run into when one chooses to pursue this approach. The solutions to these problems are also presented. In these experiments, we assembled a functional cDNA clone encoding a human leukocyte interferon a/d hybrid protein. We chose to sy作者: 抑制 時間: 2025-3-23 05:06
Assays for Gene Transferferred DNA by a Southern blot of genomic DNA or, if the transferred DNA has some unique sequence feature, by the polymerase chain reaction (PCR) carried out on genomic DNA. One may also monitor successful gene transfer by assaying for mRNA production by the exogenous DNA through Northern analysis of作者: CAJ 時間: 2025-3-23 08:51 作者: archaeology 時間: 2025-3-23 13:28 作者: 老人病學(xué) 時間: 2025-3-23 17:19 作者: nuclear-tests 時間: 2025-3-23 19:05
DNA Transfer (2) create DNA-containing complexes whose charge characteristics are compatible with DNA uptake by the target cell; or (3) result in the transient formation of pores in the plasma membrane of a cell exposed to an electric pulse, pores of sufficient size to allow DNA to enter the target cell.作者: 熱情贊揚 時間: 2025-3-23 22:48
Expression Cloningis type include selectable markers and oncogenes, as well as cytokines (Wong et al., 1985) and membrane proteins including cell-surface antigens (Aruffo and Seed, 1987) and cellular receptors (D’Andrea et al., 1989).作者: MUTED 時間: 2025-3-24 05:32 作者: 蒸發(fā) 時間: 2025-3-24 08:48 作者: ANIM 時間: 2025-3-24 11:13 作者: 苦笑 時間: 2025-3-24 16:05
Clinical Anatomy and Embryologylled incubator, an inverted microscope, a 4°C refrigerator, a ?20°C freezer, and a liquid-nitrogen freezer for long-term cell storage. It is important that the flow hood, incubator, and microscope are located such that they are separate from the facilities used for the manipulation of bacterial or y作者: 孵卵器 時間: 2025-3-24 22:55 作者: anachronistic 時間: 2025-3-25 03:11
https://doi.org/10.1007/978-981-10-0240-3tion can be infected with a recombinant retrovirus. All other means of stable transformation are less efficient. To facilitate the isolation of cells stably transformed with the DNA of interest, a gene encoding a dominant selectable marker is usually included in the transfection protocol. Such a gen作者: 小卒 時間: 2025-3-25 05:03 作者: 溫順 時間: 2025-3-25 10:20 作者: coagulation 時間: 2025-3-25 15:03
Autonomic Nervous System in the Neonateferred DNA by a Southern blot of genomic DNA or, if the transferred DNA has some unique sequence feature, by the polymerase chain reaction (PCR) carried out on genomic DNA. One may also monitor successful gene transfer by assaying for mRNA production by the exogenous DNA through Northern analysis of作者: aspect 時間: 2025-3-25 17:28 作者: obtuse 時間: 2025-3-25 21:50 作者: Cabinet 時間: 2025-3-26 04:02 作者: inventory 時間: 2025-3-26 08:22 作者: cutlery 時間: 2025-3-26 09:56 作者: 中和 時間: 2025-3-26 15:56 作者: BIPED 時間: 2025-3-26 18:52
Book 1990A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.作者: 江湖郎中 時間: 2025-3-26 22:02 作者: 圍裙 時間: 2025-3-27 03:41 作者: 具體 時間: 2025-3-27 08:46 作者: exceed 時間: 2025-3-27 09:37 作者: 圍裙 時間: 2025-3-27 16:43
Selection and Amplificationsformation rate is approximately 80 percent and if three different DNAs are transfected, including one selectable marker, the phenotypic co-transformation rate for all three is approximately 60 percent (Kriegler et al., 1983).作者: 暫停,間歇 時間: 2025-3-27 20:36 作者: inflame 時間: 2025-3-27 23:24 作者: 彎彎曲曲 時間: 2025-3-28 04:32 作者: chapel 時間: 2025-3-28 08:07
Eukaryotic Control Elements, you must familiarize yourself with these elements. Despite the apparent complexity of the interaction of these .-acting elements during the regulation of gene expression, there are a number of rules you should keep in mind to guide genetic design.作者: BIBLE 時間: 2025-3-28 14:04
Assays for Gene Expressionitates the detection of nanogram quantities of proteins in Western blotting experiments with exposure times of but a few seconds. Lastly, procedures for the analysis of post-translational modification of proteins are described.作者: Palliation 時間: 2025-3-28 18:22
Shannon R. Kotch,Matthew D. Taylorng and secretion, and (3) the biochemistry of post-translational modifications. The experimental methodology for the manipulation and analysis of these processes is described in Part III of this manual.作者: –DOX 時間: 2025-3-28 21:30
Processing of Proteins Encoded by Transferred Genesng and secretion, and (3) the biochemistry of post-translational modifications. The experimental methodology for the manipulation and analysis of these processes is described in Part III of this manual.作者: 拖債 時間: 2025-3-29 01:12 作者: 連系 時間: 2025-3-29 06:08
External Vaporizer Leak During Anesthesia,two naturally occurring interferon genes, and this approach was simpler than cloning out the two cDNAs encoding the sequences and subsequently assembling them. The completed product was a 591-bp fragment that could be further utilized by insertion into an expression plasmid or used in PCR-based expression.作者: 薄荷醇 時間: 2025-3-29 11:01 作者: 發(fā)源 時間: 2025-3-29 11:52 作者: Rodent 時間: 2025-3-29 19:39 作者: Insubordinate 時間: 2025-3-29 20:26 作者: fender 時間: 2025-3-30 01:18 作者: 枯燥 時間: 2025-3-30 06:42 作者: 公豬 時間: 2025-3-30 11:04
https://doi.org/10.1007/978-94-010-2727-4t verwunderlich, dass Unternehmen aus dem privatwirtschaftlichen Sektor zunehmend als Marktpartner für ausl?ndische Unternehmen in Betracht kommen. Die M?glichkeiten reichen dabei vom Export, der Lizenzvergabe über das Katalog- und OEM-Gesch?ft bis hin zum Joint-Venture.作者: 南極 時間: 2025-3-30 14:56 作者: 血友病 時間: 2025-3-30 19:43
2947-8847 ism? What does global rectificatory justice require in practice? The author seeks to answer these questions covering a significant gap in the literature on global justice.978-1-349-49978-6978-1-137-46612-9Series ISSN 2947-8847 Series E-ISSN 2947-8855
