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標題: Titlebook: Extracellular Matrix; Jennie B. Leach,Elizabeth M. Powell Book 2015 Springer Science+Business Media New York 2015 Bioengineering.Cell-extr [打印本頁]

作者: CRUST    時間: 2025-3-21 19:48
書目名稱Extracellular Matrix影響因子(影響力)




書目名稱Extracellular Matrix影響因子(影響力)學科排名




書目名稱Extracellular Matrix網(wǎng)絡公開度




書目名稱Extracellular Matrix網(wǎng)絡公開度學科排名




書目名稱Extracellular Matrix被引頻次




書目名稱Extracellular Matrix被引頻次學科排名




書目名稱Extracellular Matrix年度引用




書目名稱Extracellular Matrix年度引用學科排名




書目名稱Extracellular Matrix讀者反饋




書目名稱Extracellular Matrix讀者反饋學科排名





作者: Glycogen    時間: 2025-3-22 00:19

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作者: 極肥胖    時間: 2025-3-22 15:35
High-Throughput Quantitative Assay for Analyzing Neurite Outgrowth on a Uniform Substratum: The Cells time and effort. A high-throughput assay provides a quick method to obtain quantitative measures of neurite outgrowth. In this chapter, the experimental setup and imaging protocol are described to efficiently process images of primary neurons or cell lines in order to quantitate parameters.
作者: 極肥胖    時間: 2025-3-22 21:02

作者: Pamphlet    時間: 2025-3-22 23:49
Replicating the In Vivo Environment: Organotypic and Submerged Three-Dimensional Culture Methodsrely on modified two-dimensional (2D) substrates. When a cell is attached to a flat surface, the cellular structure is limited by adhesion to the substrate, and the cell is unable to acquire the in vivo morphology and possibly function. In most tissue culture applications, extracellular matrix (ECM)
作者: 侵略者    時間: 2025-3-23 04:35
Culturing Neurons, Glia, and Progenitor Cells in Three-Dimensional Hydrogelsy relevant cell responses occur in 3D culture environments as opposed to flat stiff culture substrates such as tissue culture plates and coverslips. Hydrogels provide significant advantages towards providing cells a biocompatible, all-encompassing culture environment, but pose challenges towards ada
作者: 培養(yǎng)    時間: 2025-3-23 08:03
Investigating Cell-ECM Interactions and ECM Synthesis in Three-Dimensional Hydrogelsapplied as vehicles for delivering cells for therapeutic applications in vivo. Cells secrete extracellular matrix (ECM), the composition of which depends on the type of cell and cues present; the ECM is assembled and remodeled, forming a dynamic cellular niche. While it is generally recognized that
作者: 殺子女者    時間: 2025-3-23 10:49
Characterization and Analysis of Extracellular Matrix in Malignant Brain Tumors and Their Cellular Dr the myriad of roles the ECM plays in a variety of diseases including malignant gliomas, the most prevalent and deadly primary brain tumors. Major components of the glioma ECM include secreted proteoglycans as well as basal lamina proteins such as laminins. Characterization of secreted proteoglycan
作者: CRAMP    時間: 2025-3-23 16:41
Chondroitinase Gene Therapy for Spinal Cord Injurywn to strongly participate in regenerative failure. Shortly after injury, the formation of a glial-fibroblastic scar develops at the injury site. Besides isolating the injury area and stabilizing inflammation and cellular damage, the glial scar is also an important source of both the physical and mo
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作者: 業(yè)余愛好者    時間: 2025-3-24 13:40
Jennie B. Leach,Elizabeth M. PowellIncludes cutting-edge methods and protocols involving the extracellular matrix in native, injured, and engineered neural tissues.Provides step-by-step detail for the utmost clarity.Contains some key i
作者: Arroyo    時間: 2025-3-24 16:19
Neuromethodshttp://image.papertrans.cn/e/image/319929.jpg
作者: 極微小    時間: 2025-3-24 21:50
function. Defects in basement membrane integrity are associated with a large number of brain disorders including cobblestone lissencephaly and intracerebral hemorrhage. Here, we describe immunohistochemical approaches for characterizing the neural and vascular basement membranes in the perinatal mou
作者: Ingredient    時間: 2025-3-25 02:34
https://doi.org/10.1057/978-1-137-51257-4, is specialized and contains factors that are essential for the proper development, maintenance, and regeneration of the synapse after injury. Immunohistochemical studies have been critical in characterizing the composition of the synaptic basal lamina at the NMJ. Although NMJs are extremely stable
作者: 反省    時間: 2025-3-25 04:38

作者: PHAG    時間: 2025-3-25 11:29
nd microglia. In vitro, astrocytes provide support for neuronal cultures for the study of development and regeneration of the brain. Upon injury, astrocytes can produce molecules that protect the harmed area and also prevent further regeneration. Research of astrocyte cell biology and translational
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作者: SPER    時間: 2025-3-25 18:10
Light at the End of the Tunnel?,s time and effort. A high-throughput assay provides a quick method to obtain quantitative measures of neurite outgrowth. In this chapter, the experimental setup and imaging protocol are described to efficiently process images of primary neurons or cell lines in order to quantitate parameters.
作者: 獨輪車    時間: 2025-3-25 20:23
https://doi.org/10.1007/978-3-030-19803-9exhibiting either attractive or repellent functions. Therefore, isolated embryonic spinal cord motoneurons were cultured on a three-dimensional substrate composed of different ECM molecules produced by different glia cell lines. The motoneuron-matrix co-culture could be used to analyze the modifying
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作者: 喚醒    時間: 2025-3-26 11:43
Inequality, Poverty and Development in Indiaapplied as vehicles for delivering cells for therapeutic applications in vivo. Cells secrete extracellular matrix (ECM), the composition of which depends on the type of cell and cues present; the ECM is assembled and remodeled, forming a dynamic cellular niche. While it is generally recognized that
作者: growth-factor    時間: 2025-3-26 15:26
Inequality, Poverty and Well-being,r the myriad of roles the ECM plays in a variety of diseases including malignant gliomas, the most prevalent and deadly primary brain tumors. Major components of the glioma ECM include secreted proteoglycans as well as basal lamina proteins such as laminins. Characterization of secreted proteoglycan
作者: 條約    時間: 2025-3-26 18:40
Mettray: Normalisation or Rescue?,wn to strongly participate in regenerative failure. Shortly after injury, the formation of a glial-fibroblastic scar develops at the injury site. Besides isolating the injury area and stabilizing inflammation and cellular damage, the glial scar is also an important source of both the physical and mo
作者: 輕率的你    時間: 2025-3-27 00:38

作者: 單調女    時間: 2025-3-27 04:14
https://doi.org/10.1007/978-1-4615-0673-7es to modifications in finely tuned three-dimensional (3-D) microenvironments as opposed to traditional two-dimensional (2-D) culture systems. However, moving into a 3-D system presents complications of assessing spatial and temporal alterations in cellular behavior (e.g., protein synthesis, motilit
作者: 辭職    時間: 2025-3-27 06:29
Demand Elasticities for Health Care, particular, aligned, electrospun fibers are capable of directing astrocyte cellular extension and migration. The precise mechanisms by which aligned, electrospun substrates alter glial cell behavior are poorly understood. Therefore, there is a need for designing and refining electrospun fiber platf
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作者: 宿醉    時間: 2025-3-27 13:43

作者: Maximizer    時間: 2025-3-27 18:28
function. Defects in basement membrane integrity are associated with a large number of brain disorders including cobblestone lissencephaly and intracerebral hemorrhage. Here, we describe immunohistochemical approaches for characterizing the neural and vascular basement membranes in the perinatal mouse brain.
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0893-2336 ep-by-step detail for the utmost clarity.Contains some key i.This volume provides comprehensive procedures for analyzing the extracellular matrix in native, injured, and engineered neural tissues. Divided into four parts, each focusing on different aspects of the extracellular matrix and the nervous
作者: installment    時間: 2025-3-29 10:47

作者: BLINK    時間: 2025-3-29 13:14

作者: 帶來墨水    時間: 2025-3-29 18:36
The Measurement of Income Inequality,ollagen gels. We also provide several basic methods to assess cell viability, fix and stain cells while encapsulated in the gels, and digest the gels to retrieve live cells for subculturing or other experiments (e.g., flow cytometry).
作者: Obstacle    時間: 2025-3-29 21:22
Inequality, Poverty and Development in Indiaigations have centered on quantifying these processes in 3D gel microenvironments. Herein, we provide methods to characterize the location and patterns of ECM and cell-ECM interactions using immunofluorescence and collect cell lysate for analysis via immunoblotting or ELISA.
作者: Overdose    時間: 2025-3-30 00:15
https://doi.org/10.1007/978-3-030-17111-7ee common glycan motifs present on ECM glycoproteins of the CNS. Furthermore, we outline a protocol on how to transfect neural stem cells with expression plasmids coding for individual glycosyltransferases. This is a means to modify the glycosylation of cell surface and ECM proteins and thus study the effects of protein glycosylation.
作者: congenial    時間: 2025-3-30 08:07
Culturing Neurons, Glia, and Progenitor Cells in Three-Dimensional Hydrogelsollagen gels. We also provide several basic methods to assess cell viability, fix and stain cells while encapsulated in the gels, and digest the gels to retrieve live cells for subculturing or other experiments (e.g., flow cytometry).
作者: lattice    時間: 2025-3-30 08:21
Investigating Cell-ECM Interactions and ECM Synthesis in Three-Dimensional Hydrogelsigations have centered on quantifying these processes in 3D gel microenvironments. Herein, we provide methods to characterize the location and patterns of ECM and cell-ECM interactions using immunofluorescence and collect cell lysate for analysis via immunoblotting or ELISA.
作者: narcissism    時間: 2025-3-30 16:23
Modulation of Neural Stem Cell Expressed Extracellular Matrix (ECM) by Targeting Glycosyltransferaseee common glycan motifs present on ECM glycoproteins of the CNS. Furthermore, we outline a protocol on how to transfect neural stem cells with expression plasmids coding for individual glycosyltransferases. This is a means to modify the glycosylation of cell surface and ECM proteins and thus study the effects of protein glycosylation.
作者: 尊嚴    時間: 2025-3-30 19:51

作者: gastritis    時間: 2025-3-31 00:29
https://doi.org/10.1007/978-981-99-0518-8in-R are identified in the PNNs. Here, we describe a method which allows the sequential extraction of the main matrix compartments of the CNS, ending with the isolation of the PNN matrix molecules from the normal loose extracellular matrix in the brain. Finally we describe an easy way to quantify the extracted glycans.
作者: nocturia    時間: 2025-3-31 01:58

作者: inhumane    時間: 2025-3-31 07:02
https://doi.org/10.1007/978-3-030-19803-9 effects of matrix components like chondroitin sulfate proteoglycans, bifunctional molecules with modifying properties either due to their protein backbone or the glycan portion subdividing their action into protein and the glycan parts.
作者: 世俗    時間: 2025-3-31 10:28
Perineuronal Nets: A Special Structure in the Central Nervous System Extracellular Matrixin-R are identified in the PNNs. Here, we describe a method which allows the sequential extraction of the main matrix compartments of the CNS, ending with the isolation of the PNN matrix molecules from the normal loose extracellular matrix in the brain. Finally we describe an easy way to quantify the extracted glycans.
作者: 遠足    時間: 2025-3-31 14:47





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