標題: Titlebook: Expanded Bed Chromatography; Bo Mattiasson Book 1999 Springer Science+Business Media Dordrecht 1999 Chromatography.DNA.Downstream Processi [打印本頁] 作者: chondrocyte 時間: 2025-3-21 19:01
書目名稱Expanded Bed Chromatography影響因子(影響力)
書目名稱Expanded Bed Chromatography影響因子(影響力)學(xué)科排名
書目名稱Expanded Bed Chromatography網(wǎng)絡(luò)公開度
書目名稱Expanded Bed Chromatography網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Expanded Bed Chromatography被引頻次
書目名稱Expanded Bed Chromatography被引頻次學(xué)科排名
書目名稱Expanded Bed Chromatography年度引用
書目名稱Expanded Bed Chromatography年度引用學(xué)科排名
書目名稱Expanded Bed Chromatography讀者反饋
書目名稱Expanded Bed Chromatography讀者反饋學(xué)科排名
作者: 公社 時間: 2025-3-21 21:59 作者: tangle 時間: 2025-3-22 03:36
Monitoring, modeling, and control strategies for expanded-bed adsorption processes,mise for bed-height measurement in EBA systems. Second, we present modeling strategies for bed-height dynamics due to flow rate and fluid property changes, and lastly, we show how monitoring and modeling information can be used for the control and regulation of bed expansion.作者: 宮殿般 時間: 2025-3-22 07:35 作者: compel 時間: 2025-3-22 10:13 作者: 相一致 時間: 2025-3-22 15:23 作者: 相一致 時間: 2025-3-22 19:01 作者: 拔出 時間: 2025-3-22 21:53 作者: Receive 時間: 2025-3-23 03:57 作者: Mechanics 時間: 2025-3-23 09:36 作者: 確認 時間: 2025-3-23 11:52 作者: hypnogram 時間: 2025-3-23 15:15
ious feedstocks comprising bovine albumin in the absence and presence of 20 g dry cell weight biomass litre.. Earlier classification of the simple contactor as a single-stage, well mixed fluidised bed is reviewed. The relative merits of STREAMLINE DEAE and DEAE Spherodex LS as fluidisable, anion exchange adsorbents are discussed.作者: 衣服 時間: 2025-3-23 21:52
Mitregieren im Halbschatten der Verfassung,s are Ca.- binding proteins. Upon Ca. removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique property we develop a protocol for fast and efficient purification of α-lactalbumin from milk. The use of this technique results in a reduction of the number of chromatographic purification steps.作者: 內(nèi)部 時間: 2025-3-24 02:04
,Verschiedene Einzelausführungen,significantly when elution was carried out at high liquid velocities (300 cm/h) at high sample loads. The eluted peak volume increased with liquid velocity and increased sample load. It was approx. 80% higher in expanded bed mode than in packed bed from an adsorbent completely saturated with protein eluted at 300 cm/h.作者: HARP 時間: 2025-3-24 04:14 作者: 刺激 時間: 2025-3-24 10:13
,Konflikte moderieren und l?sen,ost applications. The key to the success of this method seems to be the use of EBA matrices with a relatively low degree of expansion (i.e. a high density) at the linear flow rates employed for elution of bound product.作者: Axillary 時間: 2025-3-24 12:41 作者: BOGUS 時間: 2025-3-24 15:29 作者: Endoscope 時間: 2025-3-24 19:48 作者: dominant 時間: 2025-3-25 00:54 作者: Breach 時間: 2025-3-25 04:15 作者: SLAG 時間: 2025-3-25 08:18 作者: flavonoids 時間: 2025-3-25 13:24 作者: 木質(zhì) 時間: 2025-3-25 16:28
the possibility of protein recovery in a single step, eliminating the often costly clarification processing steps such as ultrafiltration, centrifugation, and precipitation. An obstacle to the successful commercialization of this technology is the inability to accurately monitor and control the bed 作者: Metamorphosis 時間: 2025-3-25 21:46 作者: 一大群 時間: 2025-3-26 01:19
acterised by identical dimensions, and operated at various settled bed heights with two anion exchange adsorbents. The contactors were tested with various feedstocks comprising bovine albumin in the absence and presence of 20 g dry cell weight biomass litre.. Earlier classification of the simple con作者: GRE 時間: 2025-3-26 07:00
Kompetenz: Den Alltag meistern,cribed. An expanded bed column (5 cm diameter) has been modified to allow the abstraction of liquid samples at various heights along the bed, in an automated, semi-continuous manner throughout the separation. The withdrawn samples were filtered in-line and the level of the target protein assayed by 作者: PATHY 時間: 2025-3-26 11:14
Denktaktik: Notiere Ideen sofort,iameter columns. Column misalignment of only 0.15° resulted in the reduction of the Bodenstein number from 140 to 50 for the 1 cm dia. column and from 75 to 45 for the 5 cm dia. column. This degree of misalignment was not detectable by visual assessment of adsorbent particle movement within the colu作者: 矛盾 時間: 2025-3-26 14:59
height of an expanded bed. As the adsorbent particles were fluidized, in-bed monitoring of key component concentrations during feedstock application, washing and elution was achieved by the withdrawal of liquid samples from the voids within the expanded bed through ports along the wall of the column作者: Conserve 時間: 2025-3-26 19:26 作者: 軌道 時間: 2025-3-26 23:02 作者: esculent 時間: 2025-3-27 05:12
,Konfliktpr?vention durch Leadership, allows a qualitative and quantitative understanding of the main mechanisms governing the interaction of biomass with fluidised resins. A pulse response technique was used to determine the adsorption of various cell types (yeast, Gram positive and Gram negative bacteria, mammalian cells and yeast ho作者: propose 時間: 2025-3-27 05:45 作者: Benzodiazepines 時間: 2025-3-27 12:01
Philosophien und ihr praktischer Nutzen, broad chemical stability. Moreover, combinations of small particle diameters with high densities allow for high fluidization velocities while preserving a rapid mass transfer..This approach has been implemented by combining stable porous mineral oxide of high density (2.2, 4.7, 5.7, 9.4 g/ml) with 作者: Valves 時間: 2025-3-27 17:33 作者: Pelago 時間: 2025-3-27 18:54
Ziele und Hypothesen der Forschungsstudie STREAMLINE matrix via highly stable ether linkages. Within this development project the chemical and chromatographic stability as well as the breakthrough capacity for human IgG has been studied. The chemical stability was monitored as the carbon leakage from the matrix to the storage solution, pH 作者: AVANT 時間: 2025-3-27 23:20
Praktische Anwendung des WVBSW-Modells,ography (IMAC). The 6 × His-tagged protein binds to a chelating resin charged with metal ions such as Ni., Cu. or Zn., and can therefore be separated from proteins which have lower, or no, affinity for the resin. Two recombinant proteins, a malaria transmission-blocking vaccine candidate secreted ex作者: 范圍廣 時間: 2025-3-28 04:11
Mitregieren im Halbschatten der Verfassung,be the use of expanded bed adsorption for the recovery of a-lactalbumins from defatted milk using the hydrophobic Streamline Phenyl gel. a-Lactalbumins are Ca.- binding proteins. Upon Ca. removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique pr作者: conception 時間: 2025-3-28 09:33
Grundlegung des Problemhorizontes,tation medium. The pH of the loading and elution buffer were optimised in a packed bed with cell-free culture supernatant. Bound bacteriocin was eluted with 1.0 M NaCl. The highest recovery (30%) was obtained at the lowest pH (3.6). At higher pH values the recovery was lower, namely 12%, 15% and 7% 作者: 切割 時間: 2025-3-28 10:33
Eigenschaften der Generation Y,G. from undiluted and unclarified hybridoma cell culture broth using an ion exchange matrix in expanded bed mode. The tested new sulfopropyl-based ion exchange matrix (Streamline. SP XL, Amersham Pharmacia Biotech) stands out due to its enhanced capacity compared to its precursor (Streamline. SP). D作者: 擺動 時間: 2025-3-28 16:28
978-90-481-5380-0Springer Science+Business Media Dordrecht 1999作者: 富饒 時間: 2025-3-28 20:30
https://doi.org/10.1007/978-94-017-1519-5Chromatography; DNA; Downstream Processing; Fermentation; Glucose; Scale up; gene therapy; proteins; biochem作者: 巨碩 時間: 2025-3-28 23:24
http://image.papertrans.cn/e/image/318669.jpg作者: 才能 時間: 2025-3-29 05:09 作者: Parley 時間: 2025-3-29 09:30 作者: 高歌 時間: 2025-3-29 14:46
do not significantly affect liquid dispersion. Using the adsorption of glucose-6-phosphate dehydrogenase from yeast homogenate on Streamline DEAE as a model system, comparison of breakthrough curves for runs when in-bed monitoring was and was not performed also suggested that separation efficiency 作者: conception 時間: 2025-3-29 18:10
,Konfliktpr?vention durch Leadership,all matrices while hybridoma cells attached to all the adsorbents except the protein A affinity matrix. The method presented may be employed for screening of suitable biomass/adsorbent combinations, which yield a robust and reliable initial capture step by expanded bed adsorption from unclarified fe作者: BOLUS 時間: 2025-3-29 22:07 作者: 使堅硬 時間: 2025-3-30 01:48 作者: 排斥 時間: 2025-3-30 04:07 作者: Intrepid 時間: 2025-3-30 10:48
Praktische Anwendung des WVBSW-Modells,ation or filtration. The extracellular malaria vaccine candidate was also directly captured from a high cell density yeast culture. Detailed information on the experimental work performed, and the capture processes developed, is provided.作者: palpitate 時間: 2025-3-30 16:17
Grundlegung des Problemhorizontes,and 6.0, respectively). With pediocin, produced by . ATCC 8042, 26% recovery was obtained at a pH of 6.5 during loading and elution. Low recoveries can be ascribed to non-optimal operation conditions (pH of loading and elution buffer), inactivation of the bacteriocin on a cationic resin, and the for作者: Expostulate 時間: 2025-3-30 19:18
Eigenschaften der Generation Y,ed mode no break-through of the target protein was achieved even after 6.5 mg IgG per ml matrix were applied. These results could not be reproduced in expanded bed mode with cell-free supernatant, where the dynamic capacity was found to be only 1.5 mg IgG/ml SP XL. Processing cell-containing broth r作者: Needlework 時間: 2025-3-30 22:16
On-line monitoring of the purification of GST-(His), from an unclarified , homogenate within an imm GST-(His). in the abstracted homogenate samples was measured using Zn. loaded NTA-silica as an affinity chromatographic sensor. The approach described demonstrates potential for the on-line monitoring and control of expanded bed separations and for providing a greater understanding of adsorption/de作者: defendant 時間: 2025-3-31 03:52 作者: BRAND 時間: 2025-3-31 07:44
Evaluation of the effect of in-bed sampling on expanded bed adsorption, do not significantly affect liquid dispersion. Using the adsorption of glucose-6-phosphate dehydrogenase from yeast homogenate on Streamline DEAE as a model system, comparison of breakthrough curves for runs when in-bed monitoring was and was not performed also suggested that separation efficiency 作者: 割公牛膨脹 時間: 2025-3-31 10:24
Cell/adsorbent interactions in expanded bed adsorption of proteins,all matrices while hybridoma cells attached to all the adsorbents except the protein A affinity matrix. The method presented may be employed for screening of suitable biomass/adsorbent combinations, which yield a robust and reliable initial capture step by expanded bed adsorption from unclarified fe作者: 罐里有戒指 時間: 2025-3-31 17:15 作者: DEBT 時間: 2025-3-31 18:48 作者: 大方不好 時間: 2025-4-1 00:47
Characterisation of STREAMLINETM Phenyl,. The breakthrough capacity study demonstrates the importance of the bed height for obtaining maximal dynamic capacity. Further, there is a good correlation between breakthrough data generated from packed bed and expanded bed runs.作者: Obverse 時間: 2025-4-1 03:30 作者: 銼屑 時間: 2025-4-1 07:42
Expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentatand 6.0, respectively). With pediocin, produced by . ATCC 8042, 26% recovery was obtained at a pH of 6.5 during loading and elution. Low recoveries can be ascribed to non-optimal operation conditions (pH of loading and elution buffer), inactivation of the bacteriocin on a cationic resin, and the for作者: 愚笨 時間: 2025-4-1 12:07 作者: BIBLE 時間: 2025-4-1 17:56
Expanded bed adsorption: elution in expanded bed mode,erent liquid velocities using bovine serum albumin as a model. The effect on mixing in the liquid phase and on the volume of the eluted peak were determined. Mixing in the liquid phase was almost unaffected when elution was performed at 100 cm/h, regardless of sample load. However, mixing increased 作者: 刻苦讀書 時間: 2025-4-1 19:00
