標(biāo)題: Titlebook: Epigenomics; Methods and Protocol Izuho Hatada,Takuro Horii Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice [打印本頁(yè)] 作者: LEVEE 時(shí)間: 2025-3-21 17:01
書目名稱Epigenomics影響因子(影響力)
書目名稱Epigenomics影響因子(影響力)學(xué)科排名
書目名稱Epigenomics網(wǎng)絡(luò)公開度
書目名稱Epigenomics網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Epigenomics被引頻次
書目名稱Epigenomics被引頻次學(xué)科排名
書目名稱Epigenomics年度引用
書目名稱Epigenomics年度引用學(xué)科排名
書目名稱Epigenomics讀者反饋
書目名稱Epigenomics讀者反饋學(xué)科排名
作者: 食品室 時(shí)間: 2025-3-21 20:30
https://doi.org/10.1007/3-540-11571-4igenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for quantifying distances between . gene and clusters of cofactor BRD4 using the STREAMING-tag system in mouse embryonic stem cells.作者: 發(fā)展 時(shí)間: 2025-3-22 02:01
https://doi.org/10.1007/978-3-319-08897-6ust activation of target genes even when strongly silenced. In this chapter, we describe our protocol to design, construct, and validate the TREE-mediated target gene activation in cultured human cells.作者: 態(tài)學(xué) 時(shí)間: 2025-3-22 06:04
Book 2023s across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for ep作者: 干涉 時(shí)間: 2025-3-22 10:09 作者: CREST 時(shí)間: 2025-3-22 16:51
https://doi.org/10.1007/978-1-4615-6424-9 Our protocols typically require nine cycles of polymerase chain reaction (PCR) amplification to obtain a sufficient amount of library for sequencing when 100?ng of genomic DNA is used as a starting material; moreover, it takes 3?days to complete library preparation and quality control procedures for up to eight samples.作者: CREST 時(shí)間: 2025-3-22 17:55 作者: 波動(dòng) 時(shí)間: 2025-3-23 00:27
An Authentic Account of Adam Smithilized embryos, followed by sonication. The nucleoplasmin treatment dramatically increased the efficiency of sperm chromatin solubilization, while a?relatively large amount of recombinant nucleoplasmin was required. Here, we describe an improvement of nucleoplasmin method with a less amount of recombinant protein and a shorter reaction time.作者: projectile 時(shí)間: 2025-3-23 05:25 作者: MOTTO 時(shí)間: 2025-3-23 07:32 作者: Flagging 時(shí)間: 2025-3-23 11:32 作者: 虛度 時(shí)間: 2025-3-23 17:09 作者: thwart 時(shí)間: 2025-3-23 18:53
STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear igenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for quantifying distances between . gene and clusters of cofactor BRD4 using the STREAMING-tag system in mouse embryonic stem cells.作者: deceive 時(shí)間: 2025-3-24 00:53 作者: machination 時(shí)間: 2025-3-24 02:55 作者: 傾聽 時(shí)間: 2025-3-24 09:41
Ventricular Tachycardia Ablationmary?antibody and protein?A-MNase binding steps are completed?before the cells are bound?to Concanavalin A-coated magnetic beads.?This modification?prevents crush of oocytes and early?embryos and unwanted?loss of chromatin?during CUT&RUN?procedures.作者: Efflorescent 時(shí)間: 2025-3-24 12:29 作者: Defraud 時(shí)間: 2025-3-24 16:56 作者: Compassionate 時(shí)間: 2025-3-24 22:20
https://doi.org/10.1007/978-1-4612-4948-1 to CUT&RUN and CUT&Tag, alternative technologies to ChIP-seq, allows us to identify genomic locations of SEs and their 3D genome configuration, whereby multiple SEs act in concert. We show an analysis of mouse spermatogenesis as an example of this application.作者: 背帶 時(shí)間: 2025-3-25 03:11 作者: Coordinate 時(shí)間: 2025-3-25 07:21 作者: precede 時(shí)間: 2025-3-25 07:46 作者: Medicaid 時(shí)間: 2025-3-25 14:30
DNA Methylation Analysis Using Bisulfite Pyrosequencinguseful for identifying biomarkers that could help with the diagnosis, prognosis, treatment selection, and onset risk assessment for several diseases. We describe the principles of pyrosequencing and detail a bisulfite pyrosequencing protocol based on our experience and the PyroMark Q24 User Manual.作者: 去世 時(shí)間: 2025-3-25 19:31 作者: 必死 時(shí)間: 2025-3-25 20:58
A Method for Detection of Somatic LINE-1 Insertions at the Single-Cell Level from Postmortem Human Bnrich the genomic locus of L1Hs insertions, and (5) bioinformatic analysis to detect novel somatic L1Hs insertions. This method can detect approximately 97% of L1Hs originally existing in reference human genome and approximately 10–20 newly inserted L1Hs copies in a neuronal cell of a postmortem human brain.作者: patriot 時(shí)間: 2025-3-26 01:40 作者: 爵士樂(lè) 時(shí)間: 2025-3-26 07:37
Concatenated Coiled-Coil Tag for Highly Efficient, Small Molecule-Inducible Upregulation of Endogenonomic state. In this chapter, we describe the protocol for achieving highly efficient small molecule-inducible transcriptional activation of endogenous mammalian genes, mediated by a dCas9-based system that recruits transcriptional activation domains binding to a chain of concatenated coiled-coil peptides.作者: plasma 時(shí)間: 2025-3-26 10:58 作者: 名字 時(shí)間: 2025-3-26 13:24 作者: 焦慮 時(shí)間: 2025-3-26 18:57 作者: 易碎 時(shí)間: 2025-3-26 22:23 作者: justify 時(shí)間: 2025-3-27 02:11 作者: 向下 時(shí)間: 2025-3-27 06:46
Reduced Representation Bisulfite Sequencing (RRBS)s, regardless of their DNA methylation status at the CG site—and enables the measurement of DNA methylation levels at 5%?~?10% of all CpG sites in the mammalian genome. RRBS has been utilized in a large number of studies as a cost-effective method to investigate DNA methylation patterns, mainly at g作者: 百靈鳥 時(shí)間: 2025-3-27 13:17 作者: GIST 時(shí)間: 2025-3-27 14:38 作者: Optic-Disk 時(shí)間: 2025-3-27 20:49 作者: Vital-Signs 時(shí)間: 2025-3-28 00:17
Imaging Chromatin Accessibility by Assay of Transposase-Accessible Chromatin with Visualizationadvantages to analyze chromatin accessibility begun to explore the dynamics of local chromatin structures. Here I describe protocols for Assay of Transposase-Accessible Chromatin with Visualization (ATAC-see), which allows us to analyze subnuclear localization of accessible chromatin and quantify ac作者: 巧辦法 時(shí)間: 2025-3-28 02:42 作者: 擺動(dòng) 時(shí)間: 2025-3-28 10:06 作者: 壓迫 時(shí)間: 2025-3-28 11:50 作者: 絕種 時(shí)間: 2025-3-28 18:08
Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Proteins us unable to solubilize the chromatin due to its resistance to sonication and enzymes usually used for chromatin fragmentation in somatic cells. Even when intense enzymatic treatment is applied, it appears to solubilize only certain portions of sperm chromatin presumably because of the heterogeneo作者: 就職 時(shí)間: 2025-3-28 21:42 作者: exquisite 時(shí)間: 2025-3-28 23:54
Regulation of Gene Expression Using dCas9-SunTag PlatformsA methylation and histone modifications, and epigenetic changes can play a key role in modifying gene expression. With the advent of genome editing technologies, it has become possible to manipulate the epigenome of specific genomic regions to control gene expression. In particular, CRISPR–Cas9 syst作者: 胰島素 時(shí)間: 2025-3-29 03:36 作者: 使高興 時(shí)間: 2025-3-29 09:52 作者: Anticlimax 時(shí)間: 2025-3-29 12:32
Book 2023s on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Epigenomics: Methods and Protocols. serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research..作者: 裝飾 時(shí)間: 2025-3-29 19:05
1064-3745 and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Epigenomics: Methods and Protocols. serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research..978-1-0716-2726-6978-1-0716-2724-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: RAG 時(shí)間: 2025-3-29 20:25 作者: 閑逛 時(shí)間: 2025-3-30 01:28 作者: deadlock 時(shí)間: 2025-3-30 06:51
https://doi.org/10.1007/978-1-4615-6424-9s, regardless of their DNA methylation status at the CG site—and enables the measurement of DNA methylation levels at 5%?~?10% of all CpG sites in the mammalian genome. RRBS has been utilized in a large number of studies as a cost-effective method to investigate DNA methylation patterns, mainly at g作者: UTTER 時(shí)間: 2025-3-30 09:17 作者: Cursory 時(shí)間: 2025-3-30 15:55
Rheumatic Mitral Valve Disease,e Accessible Chromatin sequencing (ATAC-seq) is one of the most popular techniques to investigate chromatin accessibility across the genome. Here we describe, step by step, a series of optimized experimental methods and bioinformatics pipelines for ATAC-seq analysis. As an example, we present an ana作者: Graphite 時(shí)間: 2025-3-30 17:04 作者: Brain-Imaging 時(shí)間: 2025-3-30 20:50 作者: BOOM 時(shí)間: 2025-3-31 02:10
https://doi.org/10.1007/3-540-11571-4 the transcription activity of a specific endogenous gene at sub-100-nm resolution in living cells. The use of this system combined with imaging of epigenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for qu作者: ethnology 時(shí)間: 2025-3-31 05:18
https://doi.org/10.1007/978-1-4612-4948-1multiple enhancers, play critical roles in regulating cell type-specific gene expression via 3D chromatin, thereby defining the cellular identities of given cells. Here we provide optimized bioinformatics pipelines to identify SEs and 3D chromatin contacts. Our pipelines encompass the processing of 作者: atopic 時(shí)間: 2025-3-31 13:14
The Classic Text: Hobbes’ Leviathancific active subtype of L1 reported to amplify its copy in neural progenitor cells causing genomic mosaicism. This chapter describes a new method named NECO-seq (Novel Elements Concentrated-sequence) to identify the genomic locus of L1Hs insertions at the single-cell level. This protocol contains th作者: 玩笑 時(shí)間: 2025-3-31 15:45
