標(biāo)題: Titlebook: Engineering and Analyzing Multicellular Systems; Methods and Protocol Lianhong Sun,Wenying Shou Book 2014 Springer Science+Business Media N [打印本頁(yè)] 作者: JADE 時(shí)間: 2025-3-21 16:15
書目名稱Engineering and Analyzing Multicellular Systems影響因子(影響力)
書目名稱Engineering and Analyzing Multicellular Systems影響因子(影響力)學(xué)科排名
書目名稱Engineering and Analyzing Multicellular Systems網(wǎng)絡(luò)公開度
書目名稱Engineering and Analyzing Multicellular Systems網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Engineering and Analyzing Multicellular Systems被引頻次
書目名稱Engineering and Analyzing Multicellular Systems被引頻次學(xué)科排名
書目名稱Engineering and Analyzing Multicellular Systems年度引用
書目名稱Engineering and Analyzing Multicellular Systems年度引用學(xué)科排名
書目名稱Engineering and Analyzing Multicellular Systems讀者反饋
書目名稱Engineering and Analyzing Multicellular Systems讀者反饋學(xué)科排名
作者: 阻擋 時(shí)間: 2025-3-21 22:44 作者: quiet-sleep 時(shí)間: 2025-3-22 02:15
Lawrence Freedman,Jeffrey Michaelspes of natural communities might have persisted and evolved. Here, we first discuss important considerations when designing a synthetically engineered system. Then, we describe the steps required to create a two-partner cooperative system based on the yeast ..作者: 飾帶 時(shí)間: 2025-3-22 07:58 作者: 急急忙忙 時(shí)間: 2025-3-22 12:11
https://doi.org/10.1057/9781137483553 changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we describe a method for transcriptome analysis of a microbial coculture in which two different cell populations are separated by a porous membrane.作者: maverick 時(shí)間: 2025-3-22 13:10 作者: maverick 時(shí)間: 2025-3-22 20:14
https://doi.org/10.1007/978-3-319-17476-1microbial communities is important for investigating metabolic exchange between species without affecting the viability of the colonies. We describe the protocol for bacterial growth, sample preparation, ambient profiling, and data analysis of microbial communities using nano-DESI MS.作者: 比目魚 時(shí)間: 2025-3-23 00:15 作者: BUMP 時(shí)間: 2025-3-23 03:56 作者: 神化怪物 時(shí)間: 2025-3-23 06:02 作者: 預(yù)兆好 時(shí)間: 2025-3-23 10:25 作者: Sedative 時(shí)間: 2025-3-23 16:38
An Observation Method for Autonomous Signaling-Mediated Synthetic Diversification in ,cation system were diversified into two distinct cell states, high and low, through autonomous signaling-mediated bifurcation, when all cells were initialized to the low state. In this chapter, we illustrate the detailed experimental procedures involved in the initialization of cells and the observation of the phenotypic diversification.作者: parasite 時(shí)間: 2025-3-23 18:18 作者: regale 時(shí)間: 2025-3-24 00:14 作者: Freeze 時(shí)間: 2025-3-24 05:42 作者: 恩惠 時(shí)間: 2025-3-24 07:16 作者: creatine-kinase 時(shí)間: 2025-3-24 13:53
Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System the interplay between the synthetic gene circuits with underline cellular processes and verify the mechanism determining the pattern formation. Here, we describe simple optical setups for quantitative measurements of the cell density and growth and spatial–temporal dynamic characterization of . pattern formation in soft agar plates.作者: HUMP 時(shí)間: 2025-3-24 16:48
Transcriptome Analysis of a Microbial Coculture in which the Cell Populations Are Separated by a Mem changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we describe a method for transcriptome analysis of a microbial coculture in which two different cell populations are separated by a porous membrane.作者: deface 時(shí)間: 2025-3-24 22:35 作者: 充氣球 時(shí)間: 2025-3-25 01:03
The Characterization of Living Bacterial Colonies Using Nanospray Desorption Electrospray Ionizationmicrobial communities is important for investigating metabolic exchange between species without affecting the viability of the colonies. We describe the protocol for bacterial growth, sample preparation, ambient profiling, and data analysis of microbial communities using nano-DESI MS.作者: ornithology 時(shí)間: 2025-3-25 06:57 作者: Nuance 時(shí)間: 2025-3-25 10:48
The Context Awareness Challenges for PIS,cation system were diversified into two distinct cell states, high and low, through autonomous signaling-mediated bifurcation, when all cells were initialized to the low state. In this chapter, we illustrate the detailed experimental procedures involved in the initialization of cells and the observation of the phenotypic diversification.作者: 使高興 時(shí)間: 2025-3-25 15:14 作者: Militia 時(shí)間: 2025-3-25 16:40 作者: 專心 時(shí)間: 2025-3-25 23:19 作者: ablate 時(shí)間: 2025-3-26 01:34
978-1-4939-5272-4Springer Science+Business Media New York 2014作者: 閑聊 時(shí)間: 2025-3-26 07:08 作者: 生銹 時(shí)間: 2025-3-26 12:12
Lianhong Sun,Wenying ShouIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Chemotherapy 時(shí)間: 2025-3-26 12:40 作者: antecedence 時(shí)間: 2025-3-26 19:01
https://doi.org/10.1007/978-1-349-04271-5 novel beneficial interactions provides insight into the mechanisms of cooperation as well as the potential to improve the productivity of industrially relevant systems. Here, we present a general scheme for evolving a mutualism from a bacterial consortium in which one species consumes the by-products of another.作者: Dysarthria 時(shí)間: 2025-3-26 22:51
https://doi.org/10.1007/978-3-031-23054-7To resolve this, we have applied synthetic biology principles to engineer human commensal microbe that can specifically sense and kill an antibiotic-resistant strain of .. In this chapter, we describe the methods used to assemble, characterize, and evaluate the effectiveness of our engineered microbe in multicellular systems.作者: limber 時(shí)間: 2025-3-27 04:20 作者: 焦慮 時(shí)間: 2025-3-27 08:44
Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species novel beneficial interactions provides insight into the mechanisms of cooperation as well as the potential to improve the productivity of industrially relevant systems. Here, we present a general scheme for evolving a mutualism from a bacterial consortium in which one species consumes the by-products of another.作者: Expurgate 時(shí)間: 2025-3-27 12:12 作者: 眨眼 時(shí)間: 2025-3-27 15:54
3D-Fluorescence In Situ Hybridization of Intact, Anaerobic Biofilms. Biofilm conformation can be altered during typical sample preparation for FISH, which can impact observations in multispecies biofilms, including the relative positions of cells. Here, we describe methods to preserve 3-D structure during FISH for visualization of an anaerobic coculture biofilm of . Hildenborough and ..作者: curettage 時(shí)間: 2025-3-27 17:51
Introduction: The First Twenty-Five Yearsticellular organisms. The consortia of microbes that reside on the human body, the human-associated microbiota, are particularly interesting as targets for forward engineering and manipulation due to their relevance in health and disease. New technologies in analysis and perturbation of the human mi作者: 討人喜歡 時(shí)間: 2025-3-28 01:41
Lawrence Freedman,Jeffrey Michaelsll retaining essential features of living organisms. The degree of control afforded by this approach has been critical in understanding how similar types of natural communities might have persisted and evolved. Here, we first discuss important considerations when designing a synthetically engineered作者: ODIUM 時(shí)間: 2025-3-28 02:46
https://doi.org/10.1007/978-1-349-04271-5 novel beneficial interactions provides insight into the mechanisms of cooperation as well as the potential to improve the productivity of industrially relevant systems. Here, we present a general scheme for evolving a mutualism from a bacterial consortium in which one species consumes the by-produc作者: DECRY 時(shí)間: 2025-3-28 08:47
Strategy for an Atomic Stalemates because of their ability to simultaneously optimize multiple tasks, to test fundamental systems science, and to understand the microbial ecology of environments like chronic wounds. Metabolic engineering, synthetic biology, and microbial ecology provide a sound scientific basis for designing, buil作者: 有雜色 時(shí)間: 2025-3-28 12:26 作者: Feigned 時(shí)間: 2025-3-28 16:30 作者: Eulogy 時(shí)間: 2025-3-28 19:37 作者: 暫時(shí)過來 時(shí)間: 2025-3-28 23:58 作者: 修改 時(shí)間: 2025-3-29 03:49
https://doi.org/10.1007/978-3-031-23054-7To resolve this, we have applied synthetic biology principles to engineer human commensal microbe that can specifically sense and kill an antibiotic-resistant strain of .. In this chapter, we describe the methods used to assemble, characterize, and evaluate the effectiveness of our engineered microb作者: Trypsin 時(shí)間: 2025-3-29 08:51 作者: biosphere 時(shí)間: 2025-3-29 13:09
https://doi.org/10.1057/9781137483553undergo dynamic changes because of cell–cell and cell–environment interactions. Transcriptome analysis allows us to study the molecular basis of these changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we作者: REP 時(shí)間: 2025-3-29 17:01 作者: 無法取消 時(shí)間: 2025-3-29 20:54
The Evolution of the British Welfare States. Biofilm conformation can be altered during typical sample preparation for FISH, which can impact observations in multispecies biofilms, including the relative positions of cells. Here, we describe methods to preserve 3-D structure during FISH for visualization of an anaerobic coculture biofilm of作者: NEXUS 時(shí)間: 2025-3-30 02:52
https://doi.org/10.1007/978-3-319-17476-1le detailed molecular characterization of living bacterial colonies directly from nutrient agar. The ability to detect molecular signatures of living microbial communities is important for investigating metabolic exchange between species without affecting the viability of the colonies. We describe t作者: bronchodilator 時(shí)間: 2025-3-30 04:35
,De Pougy’s Innovative Courtesan Fiction,ntrolling natural systems. Concurrently, the rise in open-source software has greatly increased the ease at which researchers can implement their own mathematical models. With a reasonably sound understanding of mathematics and programming skills, a researcher can quickly and easily use such tools f作者: 遠(yuǎn)地點(diǎn) 時(shí)間: 2025-3-30 10:16 作者: 禮節(jié) 時(shí)間: 2025-3-30 14:39 作者: mortuary 時(shí)間: 2025-3-30 19:52 作者: ILEUM 時(shí)間: 2025-3-30 21:10
Regulation and Public Sector Management modifications to newly reprogrammed iPSCs have to be avoided in order to meet stringent safety requirements for clinical therapy. These safety challenges can be overcome by new technology platforms that enable cellular reprogramming to iPSCs without the need to utilize either recombinant DNA or vir作者: Incorporate 時(shí)間: 2025-3-31 03:22
Joseph W. Dellapenna,Joyeeta Guptadiscovery. . is a high-performance software framework for accelerating discrete agent-based simulation of biological systems with millions to trillions of cells. Simulations of comparable scale and accuracy to those taking a week of computer time using MATLAB require just hours using . on a multicor作者: 周興旺 時(shí)間: 2025-3-31 05:48
Integration-Free Reprogramming of Human Somatic Cells to Induced Pluripotent Stem Cells (iPSCs) With modifications to newly reprogrammed iPSCs have to be avoided in order to meet stringent safety requirements for clinical therapy. These safety challenges can be overcome by new technology platforms that enable cellular reprogramming to iPSCs without the need to utilize either recombinant DNA or vir
