標(biāo)題: Titlebook: Dynein; Methods and Protocol Steven M. Markus Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springe [打印本頁] 作者: Lensometer 時(shí)間: 2025-3-21 17:02
書目名稱Dynein影響因子(影響力)
書目名稱Dynein影響因子(影響力)學(xué)科排名
書目名稱Dynein網(wǎng)絡(luò)公開度
書目名稱Dynein網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Dynein被引頻次
書目名稱Dynein被引頻次學(xué)科排名
書目名稱Dynein年度引用
書目名稱Dynein年度引用學(xué)科排名
書目名稱Dynein讀者反饋
書目名稱Dynein讀者反饋學(xué)科排名
作者: Hearten 時(shí)間: 2025-3-21 23:43
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/284356.jpg作者: 煉油廠 時(shí)間: 2025-3-22 04:25
Dynein978-1-0716-2958-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: PALMY 時(shí)間: 2025-3-22 05:33
https://doi.org/10.1007/978-1-0716-2958-1organelles; DNA origami; complex molecular machines; molecular motor; interkinetic nuclear migration; spi作者: Esophagitis 時(shí)間: 2025-3-22 12:33
978-1-0716-2960-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Detonate 時(shí)間: 2025-3-22 14:03 作者: Detonate 時(shí)間: 2025-3-22 17:47 作者: 混合物 時(shí)間: 2025-3-23 00:56 作者: 手段 時(shí)間: 2025-3-23 03:46
Elsbeth von Staehr,Hermann von Staehr termed interkinetic nuclear migration. The nucleus of postmitotic neurons derived from these neural stem cells also translocates in a saltatory manner to enable neuronal migration toward the cortical plate. In these processes, various molecular motors, including cytoplasmic dynein, myosin II, and k作者: Offensive 時(shí)間: 2025-3-23 07:31 作者: 運(yùn)氣 時(shí)間: 2025-3-23 10:59
Wissenschaftliche Reisen mit Loki Schmidtotors. However, due to the difficulty in visualizing single molecules of motor proteins in vivo, the physiological function and regulation of motors at the single-molecule level have not been studied widely. Here, we describe how highly inclined and laminated optical sheet (HILO) microscopy can be a作者: 歡樂東方 時(shí)間: 2025-3-23 15:47 作者: Osteoarthritis 時(shí)間: 2025-3-23 20:32
https://doi.org/10.1007/978-3-642-56354-6 transported along microtubules by teams of kinesin and dynein motors and their cargo-specific adaptor proteins. Observations from living cells show that organelles and vesicular cargoes exhibit diverse motility characteristics. Yet, our knowledge of the molecular mechanisms by which intracellular t作者: 無節(jié)奏 時(shí)間: 2025-3-24 01:24 作者: paroxysm 時(shí)間: 2025-3-24 05:07
https://doi.org/10.1007/978-3-642-56984-5e energy-converting mechanisms and interactions with an increasing variety of dynein cargos and regulators, rapid expression and purification of a variety of dynein proteins and fragments are important. Here we describe transient expression of cytoplasmic dynein in HEK293 cells and fast small-scale 作者: VEST 時(shí)間: 2025-3-24 10:03
https://doi.org/10.1007/978-3-642-57192-3ssays, DDB displays processive runs, diffusive episodes, and transient pauses. The switching rates and durations of the different phases can be determined by tracking gold nanoparticle-labeled DDB complexes with interferometric scattering (iSCAT) microscopy and using an algorithm to separate out dif作者: 騷擾 時(shí)間: 2025-3-24 12:31 作者: prostate-gland 時(shí)間: 2025-3-24 18:38
https://doi.org/10.1007/978-3-642-57192-3ell division. These motors experience resistance from the viscoelastic environment of the surrounding cytoplasm, and opposing forces that can originate from other motors bound to cytoskeleton. Optical trapping is the most widely used method to measure the force-generating and force-response?characte作者: 同謀 時(shí)間: 2025-3-24 19:04 作者: 高射炮 時(shí)間: 2025-3-25 02:51 作者: 種類 時(shí)間: 2025-3-25 04:17 作者: ungainly 時(shí)間: 2025-3-25 08:08
https://doi.org/10.1007/978-3-642-57192-3es. We provide updated and optimized protocols for the expression and purification of a tail-truncated single-headed . dynein, for polarity-marked MT polymerization, and for the non-covalent attachment of MTs to cover glass surfaces for the measurement of dynein-MT detachment forces.作者: fatty-streak 時(shí)間: 2025-3-25 15:08
Employing Live-Cell Imaging to Study Motor-Mediated Transport users to examine the net effect of motors and motor-regulatory proteins on organelle distribution. Together, these methods open the door to motility experiments that more fully interrogate the complex cellular environment.作者: 百靈鳥 時(shí)間: 2025-3-25 17:13
Measurements of the Force-Dependent Detachment Rates of Cytoplasmic Dynein from Microtubuleses. We provide updated and optimized protocols for the expression and purification of a tail-truncated single-headed . dynein, for polarity-marked MT polymerization, and for the non-covalent attachment of MTs to cover glass surfaces for the measurement of dynein-MT detachment forces.作者: 寵愛 時(shí)間: 2025-3-25 22:40 作者: 改正 時(shí)間: 2025-3-26 02:37
Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cellstion and manipulate downstream events. In this chapter, I will present methods for locally activating cytoplasmic dynein at the mitotic cell cortex in human cells, with a focus on how to generate knock-in cell lines and set up a microscope system.作者: GRE 時(shí)間: 2025-3-26 06:44
Reconstitution of Dynein/Dynactin Transport Using Recombinant Dynein adaptors, and endogenous dynactin and detail a protocol for the single-molecule motility assay. In this assay, microtubules are first immobilized on a coverslip. A fluorescently labeled dynein/dynactin/cargo adaptor complex is then added, allowing for the measurement of key motility parameters as the complex walks along the microtubule.作者: hermetic 時(shí)間: 2025-3-26 10:25
Fast and Easy Transient Mammalian Cell Expression and Purification of Cytoplasmic Dyneinpurification for high-throughput protein engineering. Mammalian cell expression might be generally considered to be a laborious process, but with recent technology and some simple inexpensive custom-built labware, dynein expression and purification from mammalian cells can be fast and easy.作者: achlorhydria 時(shí)間: 2025-3-26 16:25
https://doi.org/10.1007/978-3-642-57192-3external factors affect the microtubule-detachment rate and velocity of dynein. We also briefly discuss the remaining challenges and future directions of optical trapping studies of dyneins and other microtubule-based motors.作者: 胡言亂語 時(shí)間: 2025-3-26 19:33
Studying Dynein Mechanochemistry with an Optical Trapexternal factors affect the microtubule-detachment rate and velocity of dynein. We also briefly discuss the remaining challenges and future directions of optical trapping studies of dyneins and other microtubule-based motors.作者: 我還要背著他 時(shí)間: 2025-3-26 22:59 作者: 極小量 時(shí)間: 2025-3-27 03:19
Wissenschaftliche Reisen mit Loki Schmidtt the single-molecule level have not been studied widely. Here, we describe how highly inclined and laminated optical sheet (HILO) microscopy can be adapted to visualize single molecules of the motor protein cytoplasmic dynein-1 in mammalian cells with high signal-to-noise ratio and temporal resolution.作者: drusen 時(shí)間: 2025-3-27 06:37 作者: ARENA 時(shí)間: 2025-3-27 11:52 作者: Buttress 時(shí)間: 2025-3-27 16:47 作者: 向下 時(shí)間: 2025-3-27 19:43 作者: 外露 時(shí)間: 2025-3-27 23:11
High-Resolution Tracking of Dynein-Dynactin-BicD2 Complexesined by tracking gold nanoparticle-labeled DDB complexes with interferometric scattering (iSCAT) microscopy and using an algorithm to separate out different motility phases. Here we describe methods for purifying DDB complexes from brain lysate, labeling with gold nanoparticles, imaging by iSCAT, and analyzing the resulting trajectories.作者: 不溶解 時(shí)間: 2025-3-28 02:51 作者: brassy 時(shí)間: 2025-3-28 09:16
Problemstellung und Zielsetzung, can image fungal hyphae that naturally attach to the bottom of the chambers, using wide-field epifluorescence microscopes or the new Zeiss LSM 980 (with Airyscan 2) microscope. In addition to methods for preparing cells for imaging, a procedure for . transformation is also described.作者: 獨(dú)輪車 時(shí)間: 2025-3-28 10:49
Zur Beatmung der verschiedenen Wehen,tion and manipulate downstream events. In this chapter, I will present methods for locally activating cytoplasmic dynein at the mitotic cell cortex in human cells, with a focus on how to generate knock-in cell lines and set up a microscope system.作者: 粗俗人 時(shí)間: 2025-3-28 14:46
https://doi.org/10.1007/978-3-642-56533-5 adaptors, and endogenous dynactin and detail a protocol for the single-molecule motility assay. In this assay, microtubules are first immobilized on a coverslip. A fluorescently labeled dynein/dynactin/cargo adaptor complex is then added, allowing for the measurement of key motility parameters as the complex walks along the microtubule.作者: 叢林 時(shí)間: 2025-3-28 22:05
https://doi.org/10.1007/978-3-642-56984-5purification for high-throughput protein engineering. Mammalian cell expression might be generally considered to be a laborious process, but with recent technology and some simple inexpensive custom-built labware, dynein expression and purification from mammalian cells can be fast and easy.作者: 枯萎將要 時(shí)間: 2025-3-29 01:19
Using Budding Yeast as a Model to Understand Dynein-Mediated Cargo Transporthe mitotic spindle at the site of cell division. Monitoring dynein-mediated spindle movements in budding yeast provides a powerful tool for the quantitative measurements of various motility parameters, and a system with which to assess the consequence of mutations in dynein or its regulators. Here, 作者: 嫌惡 時(shí)間: 2025-3-29 06:51 作者: PATHY 時(shí)間: 2025-3-29 07:20
Single-Molecule Studies of Motor Adaptors Using Cell Lysatesous microtubule motors and additional factors that regulate motor function. Further, this system is highly tractable as individual proteins can readily be added or removed via overexpression or knockdown in cells. Here, we describe a protocol for in vitro reconstitution of motor-driven transport alo作者: Morsel 時(shí)間: 2025-3-29 13:07
Reconstitution of Organelle Transport Along Microtubules In Vitrolity of isolated cargoes along microtubules using TIRF microscopy and quantitative force measurements using an optical trap. These methods enable us to study how the sets of motors and adaptors that drive the transport of endogenous cargoes regulate their trafficking in cells.作者: 考古學(xué) 時(shí)間: 2025-3-29 18:57
Physiologie der W?rmeregulationhe mitotic spindle at the site of cell division. Monitoring dynein-mediated spindle movements in budding yeast provides a powerful tool for the quantitative measurements of various motility parameters, and a system with which to assess the consequence of mutations in dynein or its regulators. Here, 作者: avarice 時(shí)間: 2025-3-29 22:55
Elsbeth von Staehr,Hermann von Staehrs during proliferation and migration. The procedures described in this chapter enable the monitoring of cell cycle progression, mitosis, morphological changes, and migratory patterns in situ. This approach facilitates the elucidation of gene functions in cortical development and neurodevelopmental d作者: 歡笑 時(shí)間: 2025-3-30 00:51 作者: 爭論 時(shí)間: 2025-3-30 06:52
https://doi.org/10.1007/978-3-642-56354-6lity of isolated cargoes along microtubules using TIRF microscopy and quantitative force measurements using an optical trap. These methods enable us to study how the sets of motors and adaptors that drive the transport of endogenous cargoes regulate their trafficking in cells.作者: 拉開這車床 時(shí)間: 2025-3-30 11:01 作者: 我就不公正 時(shí)間: 2025-3-30 12:31
Live-Cell Imaging of Dynein-Mediated Cargo Transport in rganism used for studying dynein function and regulation in vivo. Here, we describe how we grow . strains for live-cell imaging and how we observe the dynein-mediated distribution of early endosomes and secretory vesicles. Using an on-stage incubator and culture chambers for inverted microscopes, we作者: tendinitis 時(shí)間: 2025-3-30 16:46 作者: Formidable 時(shí)間: 2025-3-31 00:24
Employing Live-Cell Imaging to Study Motor-Mediated Transport including activating adaptors and scaffolding proteins, and microtubule tracks that also provide regulatory cues. While in vitro studies are invaluable, fully replicating the physiological conditions under which motility occurs in cells is not yet possible. Here, we describe two methods that can be作者: 彩色的蠟筆 時(shí)間: 2025-3-31 04:06
Assessment of Dynein-Mediated Nuclear Migration in the Developing Cortex by Live-Tissue Microscopy termed interkinetic nuclear migration. The nucleus of postmitotic neurons derived from these neural stem cells also translocates in a saltatory manner to enable neuronal migration toward the cortical plate. In these processes, various molecular motors, including cytoplasmic dynein, myosin II, and k
