標(biāo)題: Titlebook: Drug‘DNA Interaction Protocols; Keith R. Fox Book 19971st edition Humana Press 1997 [打印本頁(yè)] 作者: ANNOY 時(shí)間: 2025-3-21 16:56
書(shū)目名稱Drug‘DNA Interaction Protocols影響因子(影響力)
作者: Costume 時(shí)間: 2025-3-21 22:25
The Ins and Outs of the Third DimensionA large category of anticancer drugs owe their cytotoxicity to their abihty to Interact with, and damage, DNA. Many agents form bulky adducts that block RNA and DNA polymerases, inhibiting transcription, and DNA rephcation.作者: 初次登臺(tái) 時(shí)間: 2025-3-22 03:11 作者: Sad570 時(shí)間: 2025-3-22 08:26 作者: 惡意 時(shí)間: 2025-3-22 11:56 作者: 高調(diào) 時(shí)間: 2025-3-22 13:03
New Window on the World with OpenGL ESe, but they are involved in the pathogenesls of the disease through the formation of immune complexes that indirectly cause tissue damage. Alternatively, the antibodies may crossreact with cell surface antigens and cause tissue damage directly. Thus, understanding the specificity of autounmune antibodies is important.作者: 高調(diào) 時(shí)間: 2025-3-22 19:27 作者: 群島 時(shí)間: 2025-3-22 21:36 作者: scotoma 時(shí)間: 2025-3-23 05:13
Strong, Em, and Other Phrase Elements the technique was developed in 1978 for studying the interaction of DNA-binding proteins with then target sites (.), it has proved invaluable for determining the sequence specificity of many small ligands作者: venous-leak 時(shí)間: 2025-3-23 08:55 作者: 藥物 時(shí)間: 2025-3-23 10:49
Strong, Em, and Other Phrase Elementsses are oxidized by the photochemically excited state of the uranyl1 ion, a very strong oxidant (.). Thus the uranyl ion is an efficient DNA photocleavage reagent (.,.) that has been used to study the sequence specific interaction with the phosphates of the DNA backbone of the respective DNA recogin作者: Polydipsia 時(shí)間: 2025-3-23 15:37
Strong, Em, and Other Phrase Elementscleic acids (.). Secondary structures of native DNA and RNA as well as ligandinduced changes in conformation can be probed by the use of a variety of chemical reagents, either in vitro with purified nucleic acids in a reconstituted acellular environment, or directly within the framework of a cell Ov作者: 條街道往前推 時(shí)間: 2025-3-23 21:49
Strong, Em, and Other Phrase Elementsively concerned their binding to naked DNA. In contrast, cellular DNA is packed into chromatm, generating higher order structures, which may alter the local DNA conformation and/or mask potential binding sites. This chapter describes the preparation of nucleosome particles containing radiolabeled DN作者: Itinerant 時(shí)間: 2025-3-23 23:54 作者: enchant 時(shí)間: 2025-3-24 04:46 作者: fringe 時(shí)間: 2025-3-24 06:51 作者: grotto 時(shí)間: 2025-3-24 14:20
Strong, Em, and Other Phrase Elementso interact with DNA, either by intercalation (e.g., doxorubicin, mitoxantrone), groove binding (e.g., distamycin), formation of adducts or crosslinks (e.g., melphalan, cisplatin, mitomycin C), or by incorporation of modified bases (e.g., 5-fluorouracil, 6-thioguanine). There has therefore been a gre作者: Radiation 時(shí)間: 2025-3-24 17:23
Strong, Em, and Other Phrase Elementsh are known to bind covalently to, and in many cases crosslink, the bases of DNA. These include the nitrogen mustard, chloroethyl-nitrosourea, triazene, and dimethanesulfonate classes of agents. Most simple alkylating agents of this type show a base specificity for guanine, binding primarily to the 作者: accordance 時(shí)間: 2025-3-24 20:15
New Window on the World with OpenGL ESe, but they are involved in the pathogenesls of the disease through the formation of immune complexes that indirectly cause tissue damage. Alternatively, the antibodies may crossreact with cell surface antigens and cause tissue damage directly. Thus, understanding the specificity of autounmune antib作者: Ventricle 時(shí)間: 2025-3-25 03:07
Further OpenGL Features and Techniquesfic antrbody. Most DNA is not immunogeinc, although DNA-binding antibodies are produced spontaneously in the autoimmune drsease systemrc lupus erythematosus (.). In general, these antibodies exhtbtt little sequence or structural specificity and, therefore, are not useful for probing chromosomes exce作者: PSA-velocity 時(shí)間: 2025-3-25 07:23
The Ins and Outs of the Third Dimension interactions frequently involve a reversible mechanism, a determination of the equilibrium binding constant can provide insight into the nature and strength of the underlying intermolecular events. Analysis of the induced spectral effects can also reveal considerable detail about the host-drug stoi作者: 貿(mào)易 時(shí)間: 2025-3-25 07:33 作者: 犬儒主義者 時(shí)間: 2025-3-25 15:26 作者: maudtin 時(shí)間: 2025-3-25 18:57
measurements both (.) the affinity (ΔG) and enthalpy change (ΔH) for the process. There are, however, many sites on the DNA lattice for interactions and assigning AH values for each IS dependent on the model used in the mathematical analysis. What can be obtained in most cases from careful calorimet作者: 冷峻 時(shí)間: 2025-3-25 23:57 作者: 惰性氣體 時(shí)間: 2025-3-26 02:30
DNase I Footprinting, the technique was developed in 1978 for studying the interaction of DNA-binding proteins with then target sites (.), it has proved invaluable for determining the sequence specificity of many small ligands作者: 反復(fù)拉緊 時(shí)間: 2025-3-26 05:34
Analysis of DNA-Binding Antibodies,e, but they are involved in the pathogenesls of the disease through the formation of immune complexes that indirectly cause tissue damage. Alternatively, the antibodies may crossreact with cell surface antigens and cause tissue damage directly. Thus, understanding the specificity of autounmune antibodies is important.作者: GET 時(shí)間: 2025-3-26 11:33 作者: staging 時(shí)間: 2025-3-26 15:08 作者: Brain-Waves 時(shí)間: 2025-3-26 20:25 作者: muster 時(shí)間: 2025-3-27 00:33 作者: 主講人 時(shí)間: 2025-3-27 03:23
Strong, Em, and Other Phrase Elements local DNA conformation and/or mask potential binding sites. This chapter describes the preparation of nucleosome particles containing radiolabeled DNA fragments that can be used as substrates for footprinting experiments.作者: 較早 時(shí)間: 2025-3-27 07:00
Quantitative DNA Footprinting,, electrophoresis in a polyacrylamide gel. If the ligand, when bound, inhibits cleavage by the probe, the oligonucleotides that terminate at the ligand binding site will be underrepresented among the products analyzed using the sequencing gel. This appears as omissions or “footprints” in the spots on the sequencing autoradiogram作者: OWL 時(shí)間: 2025-3-27 11:17
,Uranyl Photoprobing of DNA Structures and Drug–DNA Complexes,tion sites of various proteins, such as the λ-repressor/O. complex (.), RNA polymerase-.P1 promoter of . (.), RNA polymerase/cyclic AMP receptor protein (CRP)-.P2 promoter of . (.), CRP/CytR repressor-.P2 promoter of . (.), and transcription factor IIIA/. 5s internal control region (.).作者: Morbid 時(shí)間: 2025-3-27 15:34 作者: alabaster 時(shí)間: 2025-3-27 20:44 作者: Mri485 時(shí)間: 2025-3-27 22:30
The Ins and Outs of the Third Dimensionssful studies have been reported for both RNA and DNA systems, including condensed triple- and four-stranded systems. The present discussion, restricted to examples involving duplex DNA, illustrates the general principles involved in these powerful optical techniques.作者: anus928 時(shí)間: 2025-3-28 02:46
,Optical Absorbance and Fluorescence Techniques for Measuring DNA–Drug Interactions,ssful studies have been reported for both RNA and DNA systems, including condensed triple- and four-stranded systems. The present discussion, restricted to examples involving duplex DNA, illustrates the general principles involved in these powerful optical techniques.作者: DOSE 時(shí)間: 2025-3-28 07:45 作者: 吞沒(méi) 時(shí)間: 2025-3-28 13:44
https://doi.org/10.1007/978-1-4302-1921-7, electrophoresis in a polyacrylamide gel. If the ligand, when bound, inhibits cleavage by the probe, the oligonucleotides that terminate at the ligand binding site will be underrepresented among the products analyzed using the sequencing gel. This appears as omissions or “footprints” in the spots on the sequencing autoradiogram作者: Daily-Value 時(shí)間: 2025-3-28 15:39
Strong, Em, and Other Phrase Elementstion sites of various proteins, such as the λ-repressor/O. complex (.), RNA polymerase-.P1 promoter of . (.), RNA polymerase/cyclic AMP receptor protein (CRP)-.P2 promoter of . (.), CRP/CytR repressor-.P2 promoter of . (.), and transcription factor IIIA/. 5s internal control region (.).作者: 闡釋 時(shí)間: 2025-3-28 19:49 作者: 健談的人 時(shí)間: 2025-3-29 02:48
Strong, Em, and Other Phrase Elementsat effort over many years to establish where on the DNA these drugs interact, with the expectation that a good understanding of the nature of the DNA receptor site would lead to the design of a new generation of these drugs.作者: 使害羞 時(shí)間: 2025-3-29 06:39 作者: 廣口瓶 時(shí)間: 2025-3-29 09:47 作者: 態(tài)學(xué) 時(shí)間: 2025-3-29 11:26
ently available calorimeters can be used at concentrations approaching levels used in UV-visible studies (.–.). Combination of the calorimetric measurements with an analysis of the corresponding binding isotherm can provide both the enthalpy and entropy changes (ΔG = ΔH - TΔS) for attaching a drug to a DNA lattice site (.–.,.).作者: 數(shù)量 時(shí)間: 2025-3-29 16:29
,DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug–DNA Interactions,lators and nonintercalative binding agents, such as netropsin (unpublished results). This method is relatively safe for the user, as it employs less radioisotope than is required for the detection of alkylation products by conventional postlabeling or Maxam and Gilbert chemical cleavage techniques.作者: expansive 時(shí)間: 2025-3-29 20:44 作者: Hectic 時(shí)間: 2025-3-30 02:56 作者: duplicate 時(shí)間: 2025-3-30 05:58 作者: 高談闊論 時(shí)間: 2025-3-30 11:27
,A Gel Mobility Shift Assay for Probing the Effect of Drug–DNA Adducts on DNA-Binding Proteins,rizing drug-DNA interactions is to ascertain the ability of DNA binding proteins to recognize then drug-modified DNA consensus sequences (.–.). This approach is particularly relevant because such 5′untranslated regions are unwound when assembled on the nuclear matrix and thus accessrble to drugs dur作者: GENRE 時(shí)間: 2025-3-30 12:37
Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays, which show a limited discrimination between the target base in different sequence contexts, to those such as CC-1065 and talhinustine, which show a high degree of selectivity to certain unique sequences, with the corresponding avoidance of others.作者: Noisome 時(shí)間: 2025-3-30 19:48 作者: 有權(quán)威 時(shí)間: 2025-3-30 23:55
Book 19971st edition experimental techniques or to improve their current techniques will also find these powerful methods particularly helpful in elucidating the action of existing therapeutic agents, as well as in designing new anticancer drugs with improved action and reduced toxicity. .......
