標(biāo)題: Titlebook: Drosophila Oogenesis; Methods and Protocol Michelle S. Giedt,Tina L. Tootle Book 2023 The Editor(s) (if applicable) and The Author(s), unde [打印本頁] 作者: 難免 時間: 2025-3-21 20:09
書目名稱Drosophila Oogenesis影響因子(影響力)
書目名稱Drosophila Oogenesis影響因子(影響力)學(xué)科排名
書目名稱Drosophila Oogenesis網(wǎng)絡(luò)公開度
書目名稱Drosophila Oogenesis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Drosophila Oogenesis被引頻次
書目名稱Drosophila Oogenesis被引頻次學(xué)科排名
書目名稱Drosophila Oogenesis年度引用
書目名稱Drosophila Oogenesis年度引用學(xué)科排名
書目名稱Drosophila Oogenesis讀者反饋
書目名稱Drosophila Oogenesis讀者反饋學(xué)科排名
作者: Electrolysis 時間: 2025-3-21 23:20 作者: 典型 時間: 2025-3-22 02:39 作者: 謙卑 時間: 2025-3-22 07:59 作者: 喚醒 時間: 2025-3-22 10:17
Analysis of Physiological Control of Adult , Oogenesis by Interorgan Communication, sensitive to environmental challenges and has recently been shown to be regulated by signaling from peripheral organs. To dissect the intricate coordination between overall organism health and reproduction, it is necessary to meticulously characterize both experimental tools and oogenesis processes作者: 偉大 時間: 2025-3-22 13:27
Immunohistochemical Analysis of Nuclear Lamina Structures in the , Ovary Using CRISPR-Tagged Genes,ver, as females age, fecundity declines, in part, due to changes in ovarian niche function and germline stem cell (GSC) homeostasis. Understanding the dynamics of GSC maintenance will provide needed insights into how coordinated tissue homeostasis is lost during aging. Critical regulators of GSC mai作者: 偉大 時間: 2025-3-22 19:48 作者: EXUDE 時間: 2025-3-22 22:54 作者: 斜谷 時間: 2025-3-23 02:17
Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Millective migration. The basal surface of each follicle cell has lamellipodial and filopodial protrusions that extend from its leading edge and an array of stress fibers that mediate its adhesion to the extracellular matrix; these migratory structures are all globally aligned in the direction of tiss作者: 爭議的蘋果 時間: 2025-3-23 05:37 作者: 領(lǐng)帶 時間: 2025-3-23 12:56 作者: 音樂會 時間: 2025-3-23 14:28 作者: HALO 時間: 2025-3-23 21:57 作者: 思想上升 時間: 2025-3-23 22:39
A Low-Tech Flow Chamber for Live Imaging of , Egg Chambers During Drug Treatments,tem is the ability to capture these dynamic processes in ex vivo organ culture using high-resolution live imaging. Ex vivo culture also allows acute pharmacological or labeling treatments, extending the versatility of the system. However, many current ex vivo egg chamber culture setups do not permit作者: 租約 時間: 2025-3-24 03:18 作者: MUT 時間: 2025-3-24 07:07
Measuring Transposable Element Activity in Adult , Ovaries,ession of transposable elements (TEs) involves the complex PIWI pathway and several proteins associated with heterochromatinization. All players of TE repression are indispensable for proper reproductive fitness, as loss-of-function mutations in these genes result primarily in sterility and impaired作者: 文藝 時間: 2025-3-24 11:57 作者: 裁決 時間: 2025-3-24 18:21 作者: ALB 時間: 2025-3-24 22:47 作者: 軍火 時間: 2025-3-25 01:47 作者: Acumen 時間: 2025-3-25 05:06
978-1-0716-2972-7The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 占線 時間: 2025-3-25 11:10
Drosophila Oogenesis978-1-0716-2970-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: output 時間: 2025-3-25 15:12
https://doi.org/10.1007/978-1-349-15490-6is as a model tissue. We begin with an overview of ovary development and adult oogenesis. Then we summarize how the adult . ovary continues to advance our understanding of stem cells, cell cycle, cell migration, cytoplasmic streaming, nurse cell dumping, and cell death. We also review emerging areas作者: Dawdle 時間: 2025-3-25 15:57 作者: Solace 時間: 2025-3-25 22:48
https://doi.org/10.1007/978-1-349-15490-6d animal development. To characterize mutants with defects in oogenesis or to observe the distribution of gene products involved in egg production, the ovaries must be carefully extracted and prepared for analysis. This chapter describes the manual dissection of ovaries from adult . females, followe作者: grieve 時間: 2025-3-26 03:58 作者: CURB 時間: 2025-3-26 08:22 作者: Contend 時間: 2025-3-26 09:48 作者: 的事物 時間: 2025-3-26 13:43 作者: 馬具 時間: 2025-3-26 19:33 作者: 催眠 時間: 2025-3-26 23:16 作者: Genistein 時間: 2025-3-27 03:21
Exploiting the United Kingdom’s Oil Reserves Studies of live border cell cluster migration have revealed similarities with other migrating collectives, including formation and restriction of cellular protrusions to the front of the cluster, supracellular actomyosin contractility of the entire collective, and intra-collective cell motility. He作者: Cardiac 時間: 2025-3-27 06:49
Housing,ermline and somatic cells, the ease of genetic manipulation in fruit flies, and the large number of egg chambers produced by each fly. Recent improvements in live imaging and ex vivo culturing protocols have enabled researchers to conduct more detailed, longer-term studies of egg chamber development作者: hypnogram 時間: 2025-3-27 10:30 作者: dermatomyositis 時間: 2025-3-27 15:32
The Prospects for Global Energy, 1985–2000tion of multiple cellular and molecular events including the spatiotemporal breakdown of the follicle wall. Recent work has shown that ovulation in . utilizes conserved cellular processes and molecular pathways as in mammals. Thus, . ovulation can serve as a good model to decipher the fundamental me作者: Conduit 時間: 2025-3-27 21:15
The Prospects for Global Energy, 1985–2000tem is the ability to capture these dynamic processes in ex vivo organ culture using high-resolution live imaging. Ex vivo culture also allows acute pharmacological or labeling treatments, extending the versatility of the system. However, many current ex vivo egg chamber culture setups do not permit作者: 圓木可阻礙 時間: 2025-3-27 23:17
The Prospects for Global Energy, 1985–2000s, it is critical for researchers to be able to identify which of their stocks maintain this infection to avoid any potential confounding variables. Here, we describe methods for detecting the bacterium and assessing the infection, including polymerase chain reaction (PCR) of DNA, multi-locus sequen作者: 描述 時間: 2025-3-28 05:37
The Prospects for Global Energy, 1985–2000ession of transposable elements (TEs) involves the complex PIWI pathway and several proteins associated with heterochromatinization. All players of TE repression are indispensable for proper reproductive fitness, as loss-of-function mutations in these genes result primarily in sterility and impaired作者: Prognosis 時間: 2025-3-28 07:44
,,ar heterogeneity makes the ovary a rich subject of study, but also makes it challenging to identify transcriptional differences between individual cell types using methods such as bulk RNA sequencing. The development of single-cell RNA sequencing (scRNA-seq) techniques addresses this limitation by p作者: Extort 時間: 2025-3-28 11:30
,,ogenesis. In order to understand the molecular basis of chromatin-based processes, such as transcription, it is essential to be able to study how and when different proteins, such as transcription factors, histones and RNA polymerases, interact with chromatin. One of the most popular methods to stud作者: 蔑視 時間: 2025-3-28 18:03
,,ncertain. Of the cytoskeletal proteins, the activity and biological significance of nuclear actin has been the most extensively researched. It is now clear that actin performs essential tasks both in the cytoplasm and the nucleus, and that the dynamic balance between the large cytoplasmic and the si作者: hedonic 時間: 2025-3-28 20:20 作者: 側(cè)面左右 時間: 2025-3-29 01:34
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/282978.jpg作者: licence 時間: 2025-3-29 04:17
Exploiting the United Kingdom’s Oil Reservesope. We further outline steps to quantify various cellular behaviors and protein dynamics of live migrating border cells using the Fiji image processing package of ImageJ. These methods can be adapted to other migrating cell collectives in cultured tissues and organs.作者: DIS 時間: 2025-3-29 10:12
Housing,t ovaries, apply a fluorescent LD dye, and culture follicles such that they remain alive and healthy during imaging. Finally, a method is provided that employs in vivo centrifugation to assess colocalization of markers with LDs.作者: nominal 時間: 2025-3-29 14:25
Quantitative Image Analysis of Dynamic Cell Behaviors During Border Cell Migration,ope. We further outline steps to quantify various cellular behaviors and protein dynamics of live migrating border cells using the Fiji image processing package of ImageJ. These methods can be adapted to other migrating cell collectives in cultured tissues and organs.作者: expansive 時間: 2025-3-29 19:31 作者: BUMP 時間: 2025-3-29 22:38
1064-3745 expertsThis detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing作者: Liability 時間: 2025-3-30 00:05 作者: 一再遛 時間: 2025-3-30 07:07 作者: 被告 時間: 2025-3-30 11:58 作者: ANTH 時間: 2025-3-30 15:17
The Vast Utility of , Oogenesis, of study, including the roles of lipid droplets, ribosomes, and nuclear actin in egg development. Finally, we conclude by discussing the growing conservation of processes and signaling pathways that regulate oogenesis and female reproduction from flies to humans.作者: 發(fā)出眩目光芒 時間: 2025-3-30 18:56 作者: garrulous 時間: 2025-3-30 23:58 作者: monologue 時間: 2025-3-31 03:39
Detection and Assessment of , Infection,ce typing (MLST) to identify strains, western blotting for protein detection, and immunohistochemistry and fluorescence in situ hybridization (FISH) of . ovaries to visually detect . by fluorescence microscopy.作者: 處理 時間: 2025-3-31 08:33 作者: 猛烈責(zé)罵 時間: 2025-3-31 11:43
Chromatin Immunoprecipitation Experiments from , Ovaries,y this is chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Here, we describe a ChIP-seq protocol that has been optimized for . ovaries, focusing on sample preparation through preliminary data processing.作者: 利用 時間: 2025-3-31 15:48
Detection of Actin in Nuclear Protein Fraction Isolated from Adult , Ovary,gnificantly smaller nuclear actin pools is maintained by robust transport mechanisms. Therefore, the compartment-specific manipulation or investigation of actin has been an enormous challenge. Here, we present a protocol for the detection of actin in isolated nuclear protein fractions from . ovaries.作者: 前面 時間: 2025-3-31 19:05 作者: 飾帶 時間: 2025-4-1 01:41
The Prospects for Global Energy, 1985–2000ze egg chambers in an easy-to-construct flow chamber that permits imaging of the same egg chamber through repeated solution exchanges. This will allow researchers to take greater advantage of the wide variety of available pharmacological perturbations and other treatments like dyes to study dynamic processes in the egg chamber.作者: 慎重 時間: 2025-4-1 04:01
,,ovary for scRNA-seq. This protocol emphasizes a short preparation time, high cell viability, prevention of RNA-degradation, and reduction of technical variation to achieve highly reproducible single-cell profiles.
