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標題: Titlebook: Drosophila Oogenesis; Methods and Protocol Diana P. Bratu,Gerard P. McNeil Book 2015 Springer Science+Business Media New York 2015 RNA-prot [打印本頁]

作者: 戲弄    時間: 2025-3-21 19:59
書目名稱Drosophila Oogenesis影響因子(影響力)




書目名稱Drosophila Oogenesis影響因子(影響力)學(xué)科排名




書目名稱Drosophila Oogenesis網(wǎng)絡(luò)公開度




書目名稱Drosophila Oogenesis網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Drosophila Oogenesis被引頻次




書目名稱Drosophila Oogenesis被引頻次學(xué)科排名




書目名稱Drosophila Oogenesis年度引用




書目名稱Drosophila Oogenesis年度引用學(xué)科排名




書目名稱Drosophila Oogenesis讀者反饋




書目名稱Drosophila Oogenesis讀者反饋學(xué)科排名





作者: IVORY    時間: 2025-3-21 22:16
1064-3745 ation advice from the experts.This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analys
作者: 灰姑娘    時間: 2025-3-22 01:02
https://doi.org/10.1007/978-3-642-97843-2H method to label small RNAs (200–500 nucleotides in length) that are enriched in nuclear bodies in . ovaries, such as Cajal bodies (CBs) and histone locus bodies (HLBs). This technique can also be applied to other . tissues, and to abundant mRNAs such as histone transcripts.
作者: CHASE    時間: 2025-3-22 05:14

作者: seroma    時間: 2025-3-22 10:07
https://doi.org/10.1007/978-3-642-98102-9al new mRNA target(s) of the . master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA–protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.
作者: Irrepressible    時間: 2025-3-22 14:46

作者: Irrepressible    時間: 2025-3-22 17:04
https://doi.org/10.1007/978-3-642-98102-9e. The newly transcribed nascent RNA is then isolated and cloned using a small RNA cloning protocol. Although it is time-consuming, the global run-on method allows the user to profile the position, orientation and amount of transcriptionally engaged RNA polymerases across the genome, therefore providing a snapshot of genome-wide transcription.
作者: 吸氣    時間: 2025-3-22 21:15

作者: 遺傳    時間: 2025-3-23 03:48
Global Run-On Sequencing (GRO-seq) Library Preparation from , Ovaries,e. The newly transcribed nascent RNA is then isolated and cloned using a small RNA cloning protocol. Although it is time-consuming, the global run-on method allows the user to profile the position, orientation and amount of transcriptionally engaged RNA polymerases across the genome, therefore providing a snapshot of genome-wide transcription.
作者: 菊花    時間: 2025-3-23 08:57

作者: 斜谷    時間: 2025-3-23 10:38

作者: Pamphlet    時間: 2025-3-23 17:09
,Bioinformatics Analysis to Identify RNA–Protein Interactions in Oogenesis,al new mRNA target(s) of the . master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA–protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.
作者: hidebound    時間: 2025-3-23 19:52

作者: nugatory    時間: 2025-3-24 01:30

作者: hegemony    時間: 2025-3-24 05:18

作者: Influx    時間: 2025-3-24 08:01

作者: 詩集    時間: 2025-3-24 10:44
Culturing , Egg Chambers and Investigating Developmental Processes Through Live Imaging,ent microscopy. This technique is suitable for observation of early- or late-stage egg development, and can be adapted to study a variety of cellular, molecular, or developmental processes. Ongoing analysis of oogenesis in living egg chambers has tremendous potential for discovery of new developmental mechanisms.
作者: neutral-posture    時間: 2025-3-24 16:11

作者: NEG    時間: 2025-3-24 20:27

作者: acetylcholine    時間: 2025-3-24 23:38
https://doi.org/10.1007/978-3-642-97360-4. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with wide-field or confocal microscopes in either an upright or an inverted configuration.
作者: Charade    時間: 2025-3-25 04:16

作者: 譏諷    時間: 2025-3-25 11:34
https://doi.org/10.1007/978-3-642-98102-9e switches, which furthers our knowledge about cell cycle control machinery and sheds new light on potential cancer treatments. Here, we give a brief summary of cell cycle switches, the associated signaling pathways and factors, and the detailed experimental procedures used to study the cell cycle switches.
作者: JADED    時間: 2025-3-25 14:14
Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Move. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with wide-field or confocal microscopes in either an upright or an inverted configuration.
作者: 尊嚴    時間: 2025-3-25 18:20

作者: PHIL    時間: 2025-3-25 23:02

作者: aerobic    時間: 2025-3-26 02:24

作者: arsenal    時間: 2025-3-26 07:50

作者: Hiatal-Hernia    時間: 2025-3-26 09:57
https://doi.org/10.1007/978-3-642-97360-4nalyzed through microscopy or biochemical and molecular biology techniques. Here we describe the isolation of ovarian tissues, techniques to enrich for egg chambers at distinct developmental stages, preparation of protein and nucleic acid extracts, and preparation for microscopic analysis of fixed tissues.
作者: 柱廊    時間: 2025-3-26 13:32
Einmalgabetherapie mit Valproins?urees an excellent model system for visualizing the actin cytoskeleton. Here, we present methods for imaging the actin cytoskeleton in . egg chambers in both fixed samples by phalloidin staining and in live egg chambers using transgenic actin labeling tools.
作者: 減少    時間: 2025-3-26 19:10
Vom Vertriebsingenieur zum Market-Ing.pproach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond . ovaries.
作者: 反復(fù)無常    時間: 2025-3-26 21:31

作者: Graves’-disease    時間: 2025-3-27 03:05

作者: 細胞膜    時間: 2025-3-27 09:21

作者: 敘述    時間: 2025-3-27 11:20

作者: Alopecia-Areata    時間: 2025-3-27 14:48
Vom neurologischen Symptom zur Diagnosement, meiosis, cell migration, morphogenesis, cell death, intercellular signaling, mRNA localization, and translational control. This review provides a brief introduction to . oogenesis, along with a survey of its diverse biological topics and the advanced genetic tools that continue to make this a
作者: 山間窄路    時間: 2025-3-27 19:45

作者: 公豬    時間: 2025-3-28 00:52

作者: 異端邪說2    時間: 2025-3-28 05:58

作者: Mercantile    時間: 2025-3-28 10:04

作者: gene-therapy    時間: 2025-3-28 13:15

作者: META    時間: 2025-3-28 18:16
https://doi.org/10.1007/978-3-642-97360-4f cytoplasmic molecules and organelles, through which cell polarity is established and maintained. The role of microtubule-mediated transport in the asymmetric localization of axis polarity determinants, in the . oocyte, has been the subject of extensive studies in the past years. However, imaging t
作者: debble    時間: 2025-3-28 20:11

作者: 友好    時間: 2025-3-28 23:11

作者: Chauvinistic    時間: 2025-3-29 06:46

作者: Estrogen    時間: 2025-3-29 08:15

作者: 燕麥    時間: 2025-3-29 14:15

作者: ABYSS    時間: 2025-3-29 18:46
Vom Vertriebsingenieur zum Market-Ing.pproach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond . ovaries.
作者: 的是兄弟    時間: 2025-3-29 21:58

作者: 全國性    時間: 2025-3-30 01:47
https://doi.org/10.1007/978-3-642-98102-9f egg chambers in each ovariole progressively develops toward maturity. Egg chamber development consists of 14 stages. From stage 1 to stage 6 (mitotic cycle), main-body follicle cells undergo mitotic divisions. From stage 7 to stage 10a (endocycle), follicle cells cease mitosis but continue three r
作者: LITHE    時間: 2025-3-30 04:30

作者: commute    時間: 2025-3-30 11:00
https://doi.org/10.1007/978-3-642-98102-9sing or biogenesis such as splicing, polyadenylation, and translation. Here I describe a bioinformatics approach that we developed to identify potential new mRNA target(s) of the . master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigati
作者: 平靜生活    時間: 2025-3-30 15:13

作者: 入會    時間: 2025-3-30 20:19
Basic Techniques in , Ovary Preparation,nalyzed through microscopy or biochemical and molecular biology techniques. Here we describe the isolation of ovarian tissues, techniques to enrich for egg chambers at distinct developmental stages, preparation of protein and nucleic acid extracts, and preparation for microscopic analysis of fixed tissues.
作者: Yourself    時間: 2025-3-31 00:26

作者: absorbed    時間: 2025-3-31 01:09

作者: 痛苦一生    時間: 2025-3-31 06:53

作者: Wordlist    時間: 2025-3-31 09:20
Vom neurologischen Symptom zur Diagnosee adult female ovary. We then describe in practical terms how to generate mosaics with examples of cross schemes and recombining strains. We also explain how to identify the appropriate progeny and how to prepare clonal tissues for phenotypic analysis.
作者: CUB    時間: 2025-3-31 15:22

作者: HAWK    時間: 2025-3-31 17:34
Mosaic Analysis in the , Ovary,e adult female ovary. We then describe in practical terms how to generate mosaics with examples of cross schemes and recombining strains. We also explain how to identify the appropriate progeny and how to prepare clonal tissues for phenotypic analysis.
作者: 等級的上升    時間: 2025-3-31 23:35

作者: 債務(wù)    時間: 2025-4-1 03:26

作者: Pulmonary-Veins    時間: 2025-4-1 09:23





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