標(biāo)題: Titlebook: Directed Enzyme Evolution: Advances and Applications; Miguel Alcalde Book 2017 Springer International Publishing AG 2017 biocatalysis.biom [打印本頁] 作者: 軍械 時(shí)間: 2025-3-21 19:59
書目名稱Directed Enzyme Evolution: Advances and Applications影響因子(影響力)
書目名稱Directed Enzyme Evolution: Advances and Applications影響因子(影響力)學(xué)科排名
書目名稱Directed Enzyme Evolution: Advances and Applications網(wǎng)絡(luò)公開度
書目名稱Directed Enzyme Evolution: Advances and Applications網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Directed Enzyme Evolution: Advances and Applications被引頻次
書目名稱Directed Enzyme Evolution: Advances and Applications被引頻次學(xué)科排名
書目名稱Directed Enzyme Evolution: Advances and Applications年度引用
書目名稱Directed Enzyme Evolution: Advances and Applications年度引用學(xué)科排名
書目名稱Directed Enzyme Evolution: Advances and Applications讀者反饋
書目名稱Directed Enzyme Evolution: Advances and Applications讀者反饋學(xué)科排名
作者: 進(jìn)取心 時(shí)間: 2025-3-22 00:02
Discussion and Framework Development,y mutant libraries requiring minimal screening effort. This chapter constitutes a critical analysis of recent developments. Saturation mutagenesis at sites lining the binding pocket using highly reduced amino acid alphabets has emerged as the superior approach for evolving stereoselectivity.作者: 富饒 時(shí)間: 2025-3-22 03:17
Book 2017ches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art..Directed molecular evolution has become the most relia作者: Etching 時(shí)間: 2025-3-22 07:14
Recent Advances in Directed Evolution of Stereoselective Enzymes,y mutant libraries requiring minimal screening effort. This chapter constitutes a critical analysis of recent developments. Saturation mutagenesis at sites lining the binding pocket using highly reduced amino acid alphabets has emerged as the superior approach for evolving stereoselectivity.作者: Cardiac-Output 時(shí)間: 2025-3-22 11:21
Konstruktionsvalidierung durch Simulation, attributes of this versatile enzyme while reflecting on the directed evolution campaigns recently followed in our laboratory that set out to enhance the functional expression of UPO in yeast and improve the activity, as well as approximating its properties to the required industrial standards.作者: 凈禮 時(shí)間: 2025-3-22 16:36 作者: 凈禮 時(shí)間: 2025-3-22 20:52
https://doi.org/10.1007/978-3-658-03047-6iversity only. We describe and critically review recent advances in the methods for DNA assembly, random mutagenesis, focused mutagenesis, and DNA recombination. We also identify trends in these areas and highlight commercial kits that are developed to streamline and expedite these molecular biology techniques.作者: NOT 時(shí)間: 2025-3-22 23:18
https://doi.org/10.1007/978-3-531-94062-5modeling, discussing their strengths, limitations, and directions. In addition, we suggest a possible future template for . directed evolution where we underline two main points: a hierarchical computational protocol combining several different techniques and a synergic effort between simulations and experimental validation.作者: Incise 時(shí)間: 2025-3-23 05:05
Molecular Modeling in Enzyme Design, Toward In Silico Guided Directed Evolution,modeling, discussing their strengths, limitations, and directions. In addition, we suggest a possible future template for . directed evolution where we underline two main points: a hierarchical computational protocol combining several different techniques and a synergic effort between simulations and experimental validation.作者: MURKY 時(shí)間: 2025-3-23 07:53 作者: 書法 時(shí)間: 2025-3-23 10:06
Strain Development by Whole-Cell Directed Evolution,r, we summarize recent technological advances and applications of directed evolution in strain development, focusing on the efforts for accelerating evolution workflows, expanding the range of target phenotypes, and facilitating mechanistic understanding of evolved mutations.作者: frivolous 時(shí)間: 2025-3-23 16:36 作者: MAZE 時(shí)間: 2025-3-23 21:35 作者: 信任 時(shí)間: 2025-3-24 01:49
http://image.papertrans.cn/e/image/280661.jpg作者: Morphine 時(shí)間: 2025-3-24 04:18 作者: 合唱隊(duì) 時(shí)間: 2025-3-24 10:12 作者: BRINK 時(shí)間: 2025-3-24 13:25
Sprinkler Irrigation Types and Componentsderivatives are, the more complex the synthesis becomes. In this chapter, we summarize the development of an engineered enzymatic platform for synthesis of diverse tryptophan analogs. This endeavor utilizes the tryptophan synthase (TrpS) enzyme, an α.β. heterodimeric protein complex that catalyzes t作者: 空氣 時(shí)間: 2025-3-24 14:56
Uniform Irrigation with Sprinklersopment, protein-based compounds represent an important subset among these biomacromolecular drug candidates, ranging from antibodies, anticoagulants, growth factors, hormones, interferons, and interleukins to enzymes. While recombinant gene technology has traditionally played a key role in developme作者: Neuropeptides 時(shí)間: 2025-3-24 21:12 作者: 過渡時(shí)期 時(shí)間: 2025-3-25 00:01
Konstruktionsvalidierung durch Simulation,). The complicated catalytic chemistry of Rubisco slows its CO. fixation rate, allows for competitive inhibition by oxygen and permits the production of misfire products that can self-inhibit activity. Significant effort has been invested into better understanding the structure-function details of R作者: 無能力 時(shí)間: 2025-3-25 04:04
Konstruktionsvalidierung durch Simulation,ed by catalytic concentrations of H.O., which acts as both oxygen donor and as final electron acceptor, this stable, soluble, and extracellular enzyme is a potential biocatalyst for dozens of transformations that are of considerable interest in organic synthesis. In this chapter we describe the main作者: 使閉塞 時(shí)間: 2025-3-25 10:52
https://doi.org/10.1007/978-3-658-03047-6gains, oilseeds, and legumes and is indigestible by monogastric animals such as poultry and swine. Supplementation of phytase in animal feed proved to improve animal nutrition and decrease phosphorous pollution. Several phytases were discovered in the last century, and today a highly competitive mar作者: Nutrient 時(shí)間: 2025-3-25 13:04
https://doi.org/10.1007/978-3-658-03047-6traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapte作者: 大看臺(tái) 時(shí)間: 2025-3-25 19:15
https://doi.org/10.1007/978-3-658-03047-6satisfy a wide range of biotechnological applications, from synthetic biology through to industrial biocatalysis. Laboratory evolution is an iterative process, alternating between creating genetic diversity and selection/screening to identify improved variants. This book chapter focuses on genetic d作者: Perigee 時(shí)間: 2025-3-26 00:00
https://doi.org/10.1007/978-3-531-94062-5es in protein scaffolds for laboratory-directed evolution and molecular design. Furthermore, the two features are not necessarily incompatible, and proteins may simultaneously be promiscuous/flexible and highly stable. In fact, it appears plausible that the combination of the two features was not un作者: 滔滔不絕地說 時(shí)間: 2025-3-26 01:12
https://doi.org/10.1007/978-3-531-94062-5lysis. Although this technique does not require any structural/mechanistic knowledge of the system, the frequency of improved mutations is usually low. For this reason, computational tools are increasingly used to focus the search in sequence space, enhancing the efficiency of laboratory evolution. 作者: Ledger 時(shí)間: 2025-3-26 06:12
Directed Evolution of an Allosteric Tryptophan Synthase to Create a Platform for Synthesis of Noncao acids. We used directed evolution to enhance the activity of TrpB from . (.TrpB), so that it can act as a stand-alone biocatalyst. Remarkably, we found that mutational activation mimics the allosteric activation induced by binding of TrpA. Toward our goal of expanding the substrate scope of this r作者: 任意 時(shí)間: 2025-3-26 09:32
Engineering Therapeutic Enzymes,ng of therapeutic enzymes. More specifically, our review will focus on three categories of therapeutic enzymes: pharmaceutical enzymes where the protein itself constitutes the therapeutic agent, prodrug-activating enzymes where the protein indirectly triggers a clinical effect, and diagnostic enzyme作者: INCH 時(shí)間: 2025-3-26 13:41
Improving CO2 Fixation by Enhancing Rubisco Performance, focus on using .. The inherent sensitivity of . viability to the pentose sugar substrate of Rubisco, RuBP, is being exploited in an increasingly effective manner to select for Rubisco mutants with increased activity. Here we review the differing directed evolution technologies used to evolve Rubisc作者: sebaceous-gland 時(shí)間: 2025-3-26 19:30
Recent Advances in Directed Phytase Evolution and Rational Phytase Engineering,ty, pH stability, pH optima, and protease resistance have been discussed with respect to structural perspective and potential molecular mechanism for improvement. Importance of cooperative substitutions and a way to identify these interactions are discussed. Recent development in screening technolog作者: pineal-gland 時(shí)間: 2025-3-26 23:51
Resurrected Ancestral Proteins as Scaffolds for Protein Engineering,ted” ancestral proteins. The combination of the two features, i.e., the ancestral hyperstable generalist phenotype, has actually been obtained in recent studies. Ancestral protein resurrection thus emerges as a useful source of scaffolds for protein engineering.作者: 故意 時(shí)間: 2025-3-27 04:49
Book 2017from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the b作者: extemporaneous 時(shí)間: 2025-3-27 06:32
Sprinkler Irrigation Types and Componentso acids. We used directed evolution to enhance the activity of TrpB from . (.TrpB), so that it can act as a stand-alone biocatalyst. Remarkably, we found that mutational activation mimics the allosteric activation induced by binding of TrpA. Toward our goal of expanding the substrate scope of this r作者: intimate 時(shí)間: 2025-3-27 10:31 作者: 巧思 時(shí)間: 2025-3-27 15:08
Konstruktionsvalidierung durch Simulation, focus on using .. The inherent sensitivity of . viability to the pentose sugar substrate of Rubisco, RuBP, is being exploited in an increasingly effective manner to select for Rubisco mutants with increased activity. Here we review the differing directed evolution technologies used to evolve Rubisc作者: FAST 時(shí)間: 2025-3-27 17:56 作者: Exonerate 時(shí)間: 2025-3-27 22:10
https://doi.org/10.1007/978-3-531-94062-5ted” ancestral proteins. The combination of the two features, i.e., the ancestral hyperstable generalist phenotype, has actually been obtained in recent studies. Ancestral protein resurrection thus emerges as a useful source of scaffolds for protein engineering.作者: 性滿足 時(shí)間: 2025-3-28 02:18 作者: 錫箔紙 時(shí)間: 2025-3-28 09:36
Directed Enzyme Evolution: Advances and Applications作者: 安撫 時(shí)間: 2025-3-28 13:49
Directed Enzyme Evolution: Advances and Applications978-3-319-50413-1作者: 槍支 時(shí)間: 2025-3-28 15:34 作者: 鞭打 時(shí)間: 2025-3-28 21:51 作者: 事情 時(shí)間: 2025-3-28 23:40 作者: etidronate 時(shí)間: 2025-3-29 05:47 作者: collagenase 時(shí)間: 2025-3-29 08:58 作者: ligature 時(shí)間: 2025-3-29 11:58
Recent Advances in Directed Phytase Evolution and Rational Phytase Engineering,gains, oilseeds, and legumes and is indigestible by monogastric animals such as poultry and swine. Supplementation of phytase in animal feed proved to improve animal nutrition and decrease phosphorous pollution. Several phytases were discovered in the last century, and today a highly competitive mar作者: Modicum 時(shí)間: 2025-3-29 16:59
Strain Development by Whole-Cell Directed Evolution,traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapte作者: 改正 時(shí)間: 2025-3-29 22:06 作者: 不可磨滅 時(shí)間: 2025-3-30 01:20
Resurrected Ancestral Proteins as Scaffolds for Protein Engineering,es in protein scaffolds for laboratory-directed evolution and molecular design. Furthermore, the two features are not necessarily incompatible, and proteins may simultaneously be promiscuous/flexible and highly stable. In fact, it appears plausible that the combination of the two features was not un作者: 檢查 時(shí)間: 2025-3-30 07:18
Molecular Modeling in Enzyme Design, Toward In Silico Guided Directed Evolution,lysis. Although this technique does not require any structural/mechanistic knowledge of the system, the frequency of improved mutations is usually low. For this reason, computational tools are increasingly used to focus the search in sequence space, enhancing the efficiency of laboratory evolution.
