標(biāo)題: Titlebook: Difference Gel Electrophoresis; Methods and Protocol Kay Ohlendieck Book 2023Latest edition The Editor(s) (if applicable) and The Author(s) [打印本頁] 作者: 女孩 時(shí)間: 2025-3-21 19:42
書目名稱Difference Gel Electrophoresis影響因子(影響力)
書目名稱Difference Gel Electrophoresis影響因子(影響力)學(xué)科排名
書目名稱Difference Gel Electrophoresis網(wǎng)絡(luò)公開度
書目名稱Difference Gel Electrophoresis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Difference Gel Electrophoresis被引頻次
書目名稱Difference Gel Electrophoresis被引頻次學(xué)科排名
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書目名稱Difference Gel Electrophoresis年度引用學(xué)科排名
書目名稱Difference Gel Electrophoresis讀者反饋
書目名稱Difference Gel Electrophoresis讀者反饋學(xué)科排名
作者: Jingoism 時(shí)間: 2025-3-21 22:17 作者: auxiliary 時(shí)間: 2025-3-22 04:26 作者: Accede 時(shí)間: 2025-3-22 05:08
Native DIGE for Quantitative and Functional Analysis of Protein Interactomesrt functional heterogeneity. One needs to consider this aspect while studying changes in abundance and activities of proteins in response to any physiological stimulus. Abundance changes in the components of a given proteome can be best visualized and efficiently quantified using electrophoresis-bas作者: 無能的人 時(shí)間: 2025-3-22 09:28
Comparative Two-Dimensional Fluorescence Gel Electrophoresist for protein spots visualized on 2D polyacrylamide gels. This is particularly important for samples that need to be compared without the availability of replicates and thus cannot be studied using differential gel electrophoresis (DIGE). CoFGE corrects for gel-to-gel variability by co-running with 作者: 涂掉 時(shí)間: 2025-3-22 15:19 作者: 涂掉 時(shí)間: 2025-3-22 17:54
DIGE-Based Phosphoproteomic Analysisls. A standard 2D-DIGE protocol is combined with subsequent post-staining with phosphospecific fluorescent dye. The combination of these two methods complements 2D-DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitiv作者: Supplement 時(shí)間: 2025-3-22 22:15 作者: nocturia 時(shí)間: 2025-3-23 02:07
DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) bal detection of expressed proteins in formalin-fixed paraffin-embedded (FFPE) tissues and its use for biomarker discovery/identification of proteins that may contribute to cancer development and progression. Formalin fixation and paraffin embedding of tissue is the standard processing methodology p作者: Offset 時(shí)間: 2025-3-23 09:26 作者: 灰姑娘 時(shí)間: 2025-3-23 12:28
Comparative 3-Sample 2D-DIGE Analysis of Skeletal Muscles muscles are characterized by a distinctive combination of contractile cells with differing physiological and biochemical properties, it is essential to determine specific differences in the protein composition of fast, slow, and hybrid fibers. Fluorescence two-dimensional difference gel electrophor作者: Anonymous 時(shí)間: 2025-3-23 17:01
Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkerse comparative and mass spectrometry-based proteomic analysis of body fluids can be particularly instrumental for the targeted identification of novel protein biomarkers with pathological relevance. In this respect, new research efforts in biomarker discovery focus on the systematic mapping of the hu作者: 軟膏 時(shí)間: 2025-3-23 19:14 作者: Hypopnea 時(shí)間: 2025-3-24 00:34 作者: 希望 時(shí)間: 2025-3-24 03:51
DIGE Analysis of Animal Tissuesn in complex mixtures. The technique addresses some of the drawbacks of conventional 2D polyacrylamide gel electrophoresis (2D-PAGE), offering improved sensitivity, more limited experimental variation, and accurate within-gel matching. 2D-DIGE is based on direct labeling of proteins with isobaric fl作者: 繼承人 時(shí)間: 2025-3-24 08:13 作者: Aids209 時(shí)間: 2025-3-24 11:14
DIGE-Based Biomarker Discovery in Blood Cancersy to couple 2D-DIGE with additional downstream mass spectrometry-based techniques yields comprehensive workflows capable of identifying proteins of biological and clinical significance. The application of 2D-DIGE in blood cancer research has significantly contributed to the increasingly important in作者: 圣歌 時(shí)間: 2025-3-24 15:44
DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) biopsies are precious samples that can generally be acquired in very small amounts due to the invasive nature of the sample collection, mainly during surgery or biopsy. Subsequently, the amount of extracted protein can be, in many cases, very limited. The saturation 2D-DIGE technology has emerged a作者: 山崩 時(shí)間: 2025-3-24 22:57
DIGE Analysis of Animal Tissuesdividual protein spot, intensities recorded at the different wavelengths are integrated and the ratio between volumes normalized to that of the internal standard. This provides an immediate appreciation of protein amount variations under the different conditions tested. In addition, proteins of inte作者: 鑲嵌細(xì)工 時(shí)間: 2025-3-25 00:09
Book 2023Latest edition and avoiding known pitfalls...?..Authoritative and cutting-edge, .Difference Gel Electrophoresis: Methods and Protocols, Second Edition. aims to ensure successful results in the further study of this vital field...?.作者: indices 時(shí)間: 2025-3-25 04:09 作者: 燦爛 時(shí)間: 2025-3-25 07:47 作者: 人類 時(shí)間: 2025-3-25 13:23
https://doi.org/10.1007/978-94-009-7526-2 biopsies are precious samples that can generally be acquired in very small amounts due to the invasive nature of the sample collection, mainly during surgery or biopsy. Subsequently, the amount of extracted protein can be, in many cases, very limited. The saturation 2D-DIGE technology has emerged a作者: cardiopulmonary 時(shí)間: 2025-3-25 19:24 作者: 易發(fā)怒 時(shí)間: 2025-3-25 23:21 作者: Saline 時(shí)間: 2025-3-26 00:09
Kay OhlendieckIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: goodwill 時(shí)間: 2025-3-26 05:19 作者: Adulterate 時(shí)間: 2025-3-26 11:19 作者: separate 時(shí)間: 2025-3-26 16:01
978-1-0716-2833-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 蓋他為秘密 時(shí)間: 2025-3-26 18:06 作者: 臭名昭著 時(shí)間: 2025-3-26 23:29 作者: 對(duì)手 時(shí)間: 2025-3-27 02:20 作者: Statins 時(shí)間: 2025-3-27 08:32
Monetary and Financial Backgroundby fluorescent tag labeling of protein samples. Scanned images of 2D-DIGE gels show thousands of protein spots, each spot representing a single or a group of protein isoforms. By using commercially available software, each protein spot is defined by an outline, which is digitized and correlated with作者: HARD 時(shí)間: 2025-3-27 10:42
https://doi.org/10.1007/978-94-009-4966-9rt functional heterogeneity. One needs to consider this aspect while studying changes in abundance and activities of proteins in response to any physiological stimulus. Abundance changes in the components of a given proteome can be best visualized and efficiently quantified using electrophoresis-bas作者: evasive 時(shí)間: 2025-3-27 15:23
Alice M. Bobra,Wan Ying Shiu,Donald Mackayt for protein spots visualized on 2D polyacrylamide gels. This is particularly important for samples that need to be compared without the availability of replicates and thus cannot be studied using differential gel electrophoresis (DIGE). CoFGE corrects for gel-to-gel variability by co-running with 作者: 背書 時(shí)間: 2025-3-27 20:50 作者: 沙發(fā) 時(shí)間: 2025-3-28 00:00
2. The Scientific Work of Luigi Solarils. A standard 2D-DIGE protocol is combined with subsequent post-staining with phosphospecific fluorescent dye. The combination of these two methods complements 2D-DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitiv作者: 熟練 時(shí)間: 2025-3-28 04:49
https://doi.org/10.1007/978-94-009-7526-2es of blood cancers have improved our understanding of disease mechanisms and identified numerous proteins of clinical relevance. For many years, gel-based proteomic studies have aided in the discovery of novel diagnostic, prognostic, and predictive biomarkers, as well as therapeutic targets, in var作者: 搖晃 時(shí)間: 2025-3-28 07:22 作者: 范例 時(shí)間: 2025-3-28 10:28
Quantification of Circulating Proteinsrange of protein abundances in biofluids such as blood and the fact that only a small number of proteins constitute the vast majority of total blood protein mass. Various separation, depletion, enrichment, and quantitative developments coupled with improvements in gel-based protein quantification te作者: Functional 時(shí)間: 2025-3-28 15:18
Turnover and Distribution of Plasma Proteins muscles are characterized by a distinctive combination of contractile cells with differing physiological and biochemical properties, it is essential to determine specific differences in the protein composition of fast, slow, and hybrid fibers. Fluorescence two-dimensional difference gel electrophor作者: 憤世嫉俗者 時(shí)間: 2025-3-28 21:12 作者: DENT 時(shí)間: 2025-3-29 00:07
Quadrilingual Economics Dictionaryent of biomolecules, including proteins, metabolites, RNA, DNA, and microorganisms. Numerous biomolecules enter saliva from the blood by passing through the intercellular spaces, reflecting the physiological state of the body. Saliva can be collected directly or using one of the numerous devices/sys作者: 大酒杯 時(shí)間: 2025-3-29 04:31
Quadrilingual Economics Dictionaryased on two-dimensional gel electrophoresis (2D-GE) separation of fluorescently labeled protein extracts. The tagging procedures are designed to not interfere with the chemical properties of proteins with respect to their p. and electrophoretic mobility, once a proper labeling protocol is followed. 作者: DIKE 時(shí)間: 2025-3-29 10:31
Quadrilingual Economics Dictionaryn in complex mixtures. The technique addresses some of the drawbacks of conventional 2D polyacrylamide gel electrophoresis (2D-PAGE), offering improved sensitivity, more limited experimental variation, and accurate within-gel matching. 2D-DIGE is based on direct labeling of proteins with isobaric fl作者: 新字 時(shí)間: 2025-3-29 14:34 作者: photophobia 時(shí)間: 2025-3-29 17:26 作者: 剛毅 時(shí)間: 2025-3-29 19:59
Book 2023Latest editionhe development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology a作者: TEN 時(shí)間: 2025-3-30 01:07
Monetary and Financial Backgroundl difference gel electrophoresis can be combined with the systematic analysis of crucial post-translational modifications. The concept of on-membrane digestion following the electrophoretic transfer of proteins and the usefulness of comparative two-dimensional immunoblotting are discussed.作者: 焦慮 時(shí)間: 2025-3-30 07:27
Top-Down Proteomics and Comparative 2D-DIGE Analysisl difference gel electrophoresis can be combined with the systematic analysis of crucial post-translational modifications. The concept of on-membrane digestion following the electrophoretic transfer of proteins and the usefulness of comparative two-dimensional immunoblotting are discussed.作者: 膽小懦夫 時(shí)間: 2025-3-30 12:00 作者: 倒轉(zhuǎn) 時(shí)間: 2025-3-30 12:52 作者: Minatory 時(shí)間: 2025-3-30 19:46
https://doi.org/10.1007/978-94-009-7526-2 enzymes, and deubiquitinating proteases. Using fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) to detect and quantitate cellular proteins associated with the ubiquitination process will facilitate the evaluation of this post-translational modification associated with the pathophysiological phenotype.作者: 思想 時(shí)間: 2025-3-30 23:27
Quantification of Circulating Proteinser technology utilizes hexapeptide bead library with huge diversity to bind and enrich low-abundance proteins but at the same time suppresses the concentration of high-abundance proteins in subsequent analysis.作者: 考博 時(shí)間: 2025-3-31 03:49
Turnover and Distribution of Plasma Proteinsn detail. A standardized proteomic workflow is described, involving sample preparation, protein extraction, differential fluorescence labeling using a 3-CyDye system, first-dimension isoelectric focusing, second-dimension slab gel electrophoresis, 2D-DIGE image analysis, protein digestion, and mass spectrometry.作者: Little 時(shí)間: 2025-3-31 07:24
Quadrilingual Economics Dictionaryantly, saliva represents a body fluid that is continuously available for diagnostic and prognostic assessments. This chapter gives an overview of saliva proteomics, including a discussion of the usefulness of both liquid chromatography and two-dimensional gel electrophoresis for efficient protein separation in saliva proteomics.
