標題: Titlebook: Developmental Biology Protocols; Volume III Rocky S. Tuan,Cecilia W. Lo Book 2000 Humana Press 2000 [打印本頁] 作者: subcutaneous 時間: 2025-3-21 19:07
書目名稱Developmental Biology Protocols影響因子(影響力)
作者: 范例 時間: 2025-3-21 21:21
Clonal Analysis in the Examination of Gene Function in ,lineage, fate determination, autonomy of gene action and pattern formation (., .). Clonal analysis has been particularly useful for the study of genes that would be lethal in a homo-zygous mutant state; this approach also makes it possible to recover essential genes in mosaic screens (.).作者: cutlery 時間: 2025-3-22 00:51
Application of Antisense Oligodeoxynucleotides in Developing Chick EmbryosOne technique used for perturbing the level of expression of a specific gene is the application of gene-specific antisense oligodeoxyribonucleotides (ODNs). ODNs are a quick, affordable, and effective means to “knock down” the expression of a gene in order to learn more of its function in vivo. It i作者: implore 時間: 2025-3-22 06:18 作者: CARE 時間: 2025-3-22 09:38
Gene Expression Analyzed by Ribonuclease Protection Assays as well as for the analysis of mRNA and gene structure (.). This solution hybridization approach is at least 10-fold more sensitive than Northern blot analysis, and thus is useful for the evaluation of low-abundance mRNAs. Furthermore, the greater stability of the RNA duplex structure over RNA-DNA作者: observatory 時間: 2025-3-22 13:07
Relative Reverse Transcription-Polymerase Chain ReactionPCR), Northern blot analysis, primer extension, and RNA protection assays (.). Because of the PCR amplification step, RT-PCR has the important advantage that little RNA is required. In addition, RT-PCR is a relatively simple procedure that does not have an absolute requirement for the use of radiois作者: observatory 時間: 2025-3-22 19:34
Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Musuming task that necessitates the use of carefully controlled amplification procedures. Reverse transcription (RT) of the mRNA to cDNA followed by amplification using the polymerization chain reaction (RT-PCR) is widely used for simple detection purposes, but the procedure can be employed for accura作者: hemophilia 時間: 2025-3-22 21:34 作者: 可以任性 時間: 2025-3-23 03:24 作者: hyperuricemia 時間: 2025-3-23 07:04
Radio-Isotopic , Hybridization on Tissue Sectionsental studies. Different strategies are to be used for different purposes. Whole mount . hybridization allows the rapid global analysis of changes in the spatiotemporal patterns of gene expression. Its use is limited to early developmental stages owing to penetration problems with increasing size of作者: ARC 時間: 2025-3-23 12:47
mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric , Hybridization and ipts using a labeled nucleotide probe and 2. immunohistochemistry (IMH), which detects protein gene products by means of labeled primary or secondary antibodies. Because these methods may be, and often are, done with whole-mount specimens to obtain resolution at the cellular level, they are performe作者: 粗鄙的人 時間: 2025-3-23 17:33 作者: 貴族 時間: 2025-3-23 21:23
Multicolor Whole-Mount , Hybridization. Since its introduction into developmental biology (.), this procedure has become an indispensable tool to investigate gene expression; initial protocols employed tritiated probes. These procedures required very long exposure times and allowed the detection of transcripts only on tissue sections. T作者: GONG 時間: 2025-3-24 02:00 作者: Legend 時間: 2025-3-24 04:37 作者: 木訥 時間: 2025-3-24 09:35 作者: 愛了嗎 時間: 2025-3-24 13:03
Guide to Teaching Puzzle-based Learning expression to be clearly discerned (.). Similar to conventional in situ hybridization, one can determine the precise cellular distribution of expression by embedding and sectioning embryos following WMISH. For these reasons WMISH has become an essential and standard tool for studying gene expressio作者: tendinitis 時間: 2025-3-24 17:30
Bo Zhou,Curtis Maines,Stephen Tang,Qi Shi, however, where the placement of the large GFP probe has interfered with the nearby domains of proteins. For example, coupling GFP to the N-terminal region of alpha-actinin, near the actin-binding domain resulted in a GFP probe that could not bind actin, whereas GFP linked to the C-terminus of alph作者: neolith 時間: 2025-3-24 20:12 作者: filicide 時間: 2025-3-24 23:28
Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins, however, where the placement of the large GFP probe has interfered with the nearby domains of proteins. For example, coupling GFP to the N-terminal region of alpha-actinin, near the actin-binding domain resulted in a GFP probe that could not bind actin, whereas GFP linked to the C-terminus of alph作者: 精確 時間: 2025-3-25 06:21 作者: 緩解 時間: 2025-3-25 09:16 作者: 專橫 時間: 2025-3-25 13:16
Guide to Software Systems Developmentlineage, fate determination, autonomy of gene action and pattern formation (., .). Clonal analysis has been particularly useful for the study of genes that would be lethal in a homo-zygous mutant state; this approach also makes it possible to recover essential genes in mosaic screens (.).作者: 極小量 時間: 2025-3-25 16:46 作者: agenda 時間: 2025-3-25 21:44
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/270062.jpg作者: Dislocation 時間: 2025-3-26 00:43 作者: 不能逃避 時間: 2025-3-26 06:35 作者: 離開可分裂 時間: 2025-3-26 12:00
Guide to Software Systems Developmentlineage, fate determination, autonomy of gene action and pattern formation (., .). Clonal analysis has been particularly useful for the study of genes that would be lethal in a homo-zygous mutant state; this approach also makes it possible to recover essential genes in mosaic screens (.).作者: IRATE 時間: 2025-3-26 14:54 作者: arcane 時間: 2025-3-26 17:13
Orit Hazzan,Tami Lapidot,Noa Ragoniser gain or loss of function to elucidate the specific roles of their protein products. One such procedure is the introduction of antigen-specific antibodies that are capable of precisely interfering with the function of a particular protein in a specific anatomical structure of the developing embryo作者: GLUT 時間: 2025-3-26 21:04
The Variety of Data Science Learnerss as well as for the analysis of mRNA and gene structure (.). This solution hybridization approach is at least 10-fold more sensitive than Northern blot analysis, and thus is useful for the evaluation of low-abundance mRNAs. Furthermore, the greater stability of the RNA duplex structure over RNA-DNA作者: Irascible 時間: 2025-3-27 04:06 作者: Obstreperous 時間: 2025-3-27 06:33
https://doi.org/10.1007/978-3-031-24758-3suming task that necessitates the use of carefully controlled amplification procedures. Reverse transcription (RT) of the mRNA to cDNA followed by amplification using the polymerization chain reaction (RT-PCR) is widely used for simple detection purposes, but the procedure can be employed for accura作者: Sarcoma 時間: 2025-3-27 11:01 作者: 珍奇 時間: 2025-3-27 15:55 作者: Nerve-Block 時間: 2025-3-27 19:24
Guide to Teaching Puzzle-based Learningental studies. Different strategies are to be used for different purposes. Whole mount . hybridization allows the rapid global analysis of changes in the spatiotemporal patterns of gene expression. Its use is limited to early developmental stages owing to penetration problems with increasing size of作者: maudtin 時間: 2025-3-28 01:59 作者: 圍裙 時間: 2025-3-28 04:59 作者: Exclude 時間: 2025-3-28 06:42
Alexandros Chrysikos,Stephen McGuire. Since its introduction into developmental biology (.), this procedure has become an indispensable tool to investigate gene expression; initial protocols employed tritiated probes. These procedures required very long exposure times and allowed the detection of transcripts only on tissue sections. T作者: Fibrillation 時間: 2025-3-28 10:33
Masquerade Detection on Mobile Devicesle mount . hybridization and immunocytochemistry allows for simultaneous detection of messenger ribonucleic acid (mRNA) and protein. The protocol for combined whole mount . hybridization and β-galactosidase staining allows for simultaneous detection of mRNA and transgene-directed β-galactosidase exp作者: Incorporate 時間: 2025-3-28 17:40 作者: collagen 時間: 2025-3-28 19:52 作者: 注入 時間: 2025-3-28 23:05
Application of Antisense Oligodeoxynucleotides in Developing Chick Embryoss a particularly useful tool to study the function of a gene in a specific target tissue, as it acts in a spatially (i.e., site of injection) and temporally (i.e., time of treatment) restricted manner.作者: Decimate 時間: 2025-3-29 03:44
Relative Reverse Transcription-Polymerase Chain Reactionotopes. Northern blot analysis requires at least ten times as much sample, radioisotopes, and the results obtained are, at best, semiquantitative. Northern blot analysis also is technically more difficult since electrophoresis of mRNA, a blotting step, and autoradiography are required.作者: FLINT 時間: 2025-3-29 09:45 作者: 一個攪動不安 時間: 2025-3-29 12:55 作者: 確定無疑 時間: 2025-3-29 15:55 作者: 有常識 時間: 2025-3-29 20:25
Methods for Double Detection of Gene Expressioncombined whole mount . hybridization and β-galactosidase staining allows for simultaneous detection of mRNA and transgene-directed β-galactosidase expression. Simultaneous detection allows for the most direct comparison of expression patterns. These procedures are derived from protocols used in . (.) and mice (.,.).作者: FLUSH 時間: 2025-3-30 03:20
https://doi.org/10.1007/978-1-4471-6799-0 study of development, a panoramic collection of experimental models of morphogenesis, development, and cellular differentiation are detailed. Both in vivo and in vitro systems are included. The volume concludes with various examples of developmental models of diseases and their molecular basis.作者: BILL 時間: 2025-3-30 05:10
Guide to Teaching Computer Sciences a particularly useful tool to study the function of a gene in a specific target tissue, as it acts in a spatially (i.e., site of injection) and temporally (i.e., time of treatment) restricted manner.作者: 持久 時間: 2025-3-30 11:37 作者: 甜食 時間: 2025-3-30 12:54
The Interdisciplinarity Challengection is relatively rare. An interesting observation is that not all placentae of HIV-1-infected women are infected (.–.). This raises the possibility that understanding how the placenta becomes infected will give insight into mechanisms of vertical transmission and may lead to therapies to prevent infection.作者: constellation 時間: 2025-3-30 20:09
https://doi.org/10.1007/978-3-031-24758-3 amplification efficiency of the PCR is rarely 100% and often far lower and it is the primer pair that contributes the most towards PCR efficiency. In addition, because the RT step is also well under 100% efficient, it is highly desirable that the control be in the form of an RNA and that it be added to the total RNA before RT.作者: 我吃花盤旋 時間: 2025-3-30 21:54
Guide to Teaching Puzzle-based Learningf early embryonic stages, it is our experience that the sensitivity of nonradioactive . hybridization on sections is inferior to that of the radio-isotopic procedure. Moreover, the sensitivity . ms probe-dependent and the signal is difficult to quantify.作者: SYN 時間: 2025-3-31 02:46
Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Mu amplification efficiency of the PCR is rarely 100% and often far lower and it is the primer pair that contributes the most towards PCR efficiency. In addition, because the RT step is also well under 100% efficient, it is highly desirable that the control be in the form of an RNA and that it be added to the total RNA before RT.作者: 古董 時間: 2025-3-31 07:21 作者: 繁榮中國 時間: 2025-3-31 12:04
1064-3745 e the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework t作者: Rebate 時間: 2025-3-31 15:39
Orit Hazzan,Tami Lapidot,Noa Ragonisbodies that are capable of precisely interfering with the function of a particular protein in a specific anatomical structure of the developing embryo. This technique, when performed correctly, proves to be a very powerful tool when investigating the presumed function of a specific protein.作者: 是限制 時間: 2025-3-31 18:08
