標題: Titlebook: Deadenylation; Methods and Protocol Eugene Valkov,Aaron C. Goldstrohm Book 2024 This is a U.S. government work and not under copyright prot [打印本頁] 作者: Scuttle 時間: 2025-3-21 19:57
書目名稱Deadenylation影響因子(影響力)
書目名稱Deadenylation影響因子(影響力)學科排名
書目名稱Deadenylation網(wǎng)絡公開度
書目名稱Deadenylation網(wǎng)絡公開度學科排名
書目名稱Deadenylation被引頻次
書目名稱Deadenylation被引頻次學科排名
書目名稱Deadenylation年度引用
書目名稱Deadenylation年度引用學科排名
書目名稱Deadenylation讀者反饋
書目名稱Deadenylation讀者反饋學科排名
作者: apropos 時間: 2025-3-21 20:57 作者: 煩擾 時間: 2025-3-22 01:17
Embarking on the Digital Odysseyhe tail, and quantify transcript abundance analogous to traditional RNAseq methods. Here, we discuss the method for generating, sequencing, and primary analysis of poly(A) tail data from total RNA using the Pacific Biosciences Sequel platform.作者: 單調(diào)性 時間: 2025-3-22 07:08 作者: anesthesia 時間: 2025-3-22 10:36
Quantification of Poly(A) Tail Length and Terminal Modifications Using Direct RNA Sequencing, cover how to prepare the libraries and perform the bioinformatics analysis to simultaneously determine the length of the transcripts’ poly(A) tails and detect the presence of terminal guanylation and uridylation.作者: 軌道 時間: 2025-3-22 13:43 作者: 軌道 時間: 2025-3-22 20:13 作者: hurricane 時間: 2025-3-22 23:48
https://doi.org/10.1007/978-3-031-70807-7inclusive full-length RNA isoform-sequencing (PAIso-seq), a method that can measure transcriptome-wide poly(A) tails from as low as nanogram level of total RNA based on the PacBio HiFi sequencing platform. The accurate length and base composition of poly(A) tails can be obtained along with the full-length cDNA.作者: Humble 時間: 2025-3-23 03:55 作者: Infiltrate 時間: 2025-3-23 07:52 作者: Conclave 時間: 2025-3-23 11:02 作者: Ischemia 時間: 2025-3-23 17:10 作者: 火海 時間: 2025-3-23 20:54 作者: REP 時間: 2025-3-23 23:02 作者: 赦免 時間: 2025-3-24 02:57 作者: remission 時間: 2025-3-24 08:30
1064-3745 leshooting and avoiding known pitfalls...?..Authoritative and cutting-edge,?.Deadenylation: Methods and Protocols .aims to pave the way for future investigations of the complex regulatory networks that control mRNA stability and expression..978-1-0716-3483-7978-1-0716-3481-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 微生物 時間: 2025-3-24 13:40
Active, Recruiting Gene Therapy Studiesning fluorimetry (thermal shift assay) as a complementary assay that allows the direct characterization of ligand binding to deadenylase enzymes. The assays can be useful for the characterization of deadenylase variants and are particularly suitable for the discovery and development of small-molecule inhibitors of deadenylase enzymes.作者: 售穴 時間: 2025-3-24 17:15
Franco M. Borrelli,Cecilia ChalliolA’s expression precisely and restores deficient protein levels to normal. This approach effectively increases the steady-state expression level of several transcripts associated with haploinsufficiency disorders in cell culture.作者: paradigm 時間: 2025-3-24 22:48 作者: 無政府主義者 時間: 2025-3-25 00:25 作者: commune 時間: 2025-3-25 05:30
Sequencing of Transcriptome-Wide Poly(A) Tails by PAIso-seq,inclusive full-length RNA isoform-sequencing (PAIso-seq), a method that can measure transcriptome-wide poly(A) tails from as low as nanogram level of total RNA based on the PacBio HiFi sequencing platform. The accurate length and base composition of poly(A) tails can be obtained along with the full-length cDNA.作者: 泥沼 時間: 2025-3-25 09:00
Differential Poly(A) Tail Length Analysis Using Nanopore Sequencing,senger RNAs. As a result, an accurate measurement of poly(A) tail length changes is important for understanding its regulatory function in different cellular contexts. Here, we outline a method for using nanopore sequencing and linear mixed models to analyze differences in poly(A) tail length across conditions.作者: 憎惡 時間: 2025-3-25 14:53 作者: liposuction 時間: 2025-3-25 17:03 作者: nullify 時間: 2025-3-25 22:55
Gonzalo L. Villarreal,Verónica Artolas, with or without poly(A) tails. Finally, we also demonstrate the adaptability of these assays to test purified protein components in our cell-free deadenylation assay. In our experience, these methods are well suited for the initial assessment of the effects of RBPs on the deadenylation of mRNAs.作者: 睨視 時間: 2025-3-26 03:59 作者: Institution 時間: 2025-3-26 04:21 作者: Baffle 時間: 2025-3-26 12:08 作者: 柔美流暢 時間: 2025-3-26 12:52 作者: incisive 時間: 2025-3-26 19:51 作者: 恩惠 時間: 2025-3-26 21:41 作者: 打火石 時間: 2025-3-27 03:34 作者: 寬敞 時間: 2025-3-27 08:04
Gonzalo L. Villarreal,Verónica Artolaics. However, direct RNA sequencing using the Oxford Nanopore Technologies (ONT) platform makes it possible to conduct transcriptome-wide analyses at the single-molecule level without the PCR bias introduced by other methods. In this chapter, we provide a protocol to measure both RNA levels and poly(A)-tail lengths in the yeast . using ONT.作者: AWE 時間: 2025-3-27 10:07 作者: 細節(jié) 時間: 2025-3-27 14:05 作者: 乳白光 時間: 2025-3-27 19:51 作者: Immobilize 時間: 2025-3-27 23:15
https://doi.org/10.1007/978-3-031-58799-3 shortening of these poly(A) tails by deadenylase enzymes has a critical role in post-transcriptional gene regulation. However, deadenylases are usually large, multisubunit, and multifunctional complexes, which complicates their biochemical analysis. This chapter presents a methodology for isolating作者: synovial-joint 時間: 2025-3-28 05:29 作者: chalice 時間: 2025-3-28 10:02
Active, Not Recruiting Studies,osine (poly(A)) RNA tract, thereby providing insight into the mechanism of deadenylation. Importantly, this assay can be performed in both homogeneous and heterogeneous environments without relying on gel electrophoresis of RNA products or coupled enzymatic reactions that indirectly report on the RN作者: EXCEL 時間: 2025-3-28 13:07 作者: 襲擊 時間: 2025-3-28 17:33 作者: 世俗 時間: 2025-3-28 22:46 作者: 獸皮 時間: 2025-3-29 01:41
Gonzalo L. Villarreal,Verónica Artolager (or noncoding) RNA through a selective, high-affinity interaction. We share a strategy for evaluating the contribution of protein positioning within an mRNA on gene expression. We introduced an RNA hairpin recognition site for the MS2 coat protein into the untranslated regions or coding sequence作者: 刺耳 時間: 2025-3-29 05:57 作者: 小隔間 時間: 2025-3-29 10:33 作者: 迫擊炮 時間: 2025-3-29 12:20 作者: DAUNT 時間: 2025-3-29 16:22 作者: Ccu106 時間: 2025-3-29 23:34
Federico Walas Mateo,Armando De Giustithat recruit or impede deadenylases, by the?incorporation of non-adenosines during poly(A) tail synthesis, and by the posttranscriptional addition of 3′ nucleotides to poly(A) tails. Deciphering the regulation of poly(A) tail shortening requires both transcriptome-wide approaches and more targeted m作者: CLOWN 時間: 2025-3-30 02:35
Gonzalo L. Villarreal,Verónica Artolamical methods to measure poly(A) tail length allow for the study of selected transcripts, the advent of long-read sequencing technologies enabled the development of simple and robust protocols to measure poly(A) tail length at the transcriptome level. Here, we describe a direct RNA sequencing protoc作者: 基因組 時間: 2025-3-30 07:13
,Bedeutung für die Coaching-Praxis,y(A) tail length play a significant role in regulating post-transcriptional gene expression by regulating the stability, translation, and decay of messenger RNAs. As a result, an accurate measurement of poly(A) tail length changes is important for understanding its regulatory function in different c作者: synchronous 時間: 2025-3-30 11:52 作者: CLOWN 時間: 2025-3-30 12:42 作者: 個阿姨勾引你 時間: 2025-3-30 16:48
Eugene Valkov,Aaron C. GoldstrohmIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: VEN 時間: 2025-3-30 21:42
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/263953.jpg作者: Comedienne 時間: 2025-3-31 03:24
Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.作者: 物種起源 時間: 2025-3-31 07:51
Transcriptome-Wide Analysis of mRNA Adenylation Status in Yeast Using Nanopore Sequencing,ics. However, direct RNA sequencing using the Oxford Nanopore Technologies (ONT) platform makes it possible to conduct transcriptome-wide analyses at the single-molecule level without the PCR bias introduced by other methods. In this chapter, we provide a protocol to measure both RNA levels and poly(A)-tail lengths in the yeast . using ONT.作者: 演繹 時間: 2025-3-31 11:13
Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.作者: 百靈鳥 時間: 2025-3-31 15:11 作者: Yourself 時間: 2025-3-31 19:14 作者: –FER 時間: 2025-3-31 23:10 作者: CAMP 時間: 2025-4-1 05:42 作者: 惰性女人 時間: 2025-4-1 09:04
