標題: Titlebook: DNAzymes; Methods and Protocol Gerhard Steger,Hannah Rosenbach,Ingrid Span Book 2022 The Editor(s) (if applicable) and The Author(s), under [打印本頁] 作者: Falter 時間: 2025-3-21 18:27
書目名稱DNAzymes影響因子(影響力)
書目名稱DNAzymes影響因子(影響力)學(xué)科排名
書目名稱DNAzymes網(wǎng)絡(luò)公開度
書目名稱DNAzymes網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱DNAzymes被引頻次
書目名稱DNAzymes被引頻次學(xué)科排名
書目名稱DNAzymes年度引用
書目名稱DNAzymes年度引用學(xué)科排名
書目名稱DNAzymes讀者反饋
書目名稱DNAzymes讀者反饋學(xué)科排名
作者: APEX 時間: 2025-3-21 22:59 作者: Sinus-Node 時間: 2025-3-22 02:05 作者: 是突襲 時間: 2025-3-22 04:56
https://doi.org/10.1007/978-3-031-00119-2inciple also feasible. Moreover, it sets the path to label, for example, long riboswitches, ribozymes, and DNAzymes for coarse-grained structure determination and enables to investigate mechanistical features of these systems.作者: Crater 時間: 2025-3-22 09:28
Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymes作者: neurologist 時間: 2025-3-22 14:04 作者: neurologist 時間: 2025-3-22 20:38 作者: Lamina 時間: 2025-3-22 21:38
Spin Labeling of Long RNAs Via Click Reaction and Enzymatic Ligationinciple also feasible. Moreover, it sets the path to label, for example, long riboswitches, ribozymes, and DNAzymes for coarse-grained structure determination and enables to investigate mechanistical features of these systems.作者: 消瘦 時間: 2025-3-23 03:39 作者: FUSC 時間: 2025-3-23 08:35 作者: indoctrinate 時間: 2025-3-23 13:34 作者: 貧窮地活 時間: 2025-3-23 14:04
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260232.jpg作者: 調(diào)整 時間: 2025-3-23 21:24
https://doi.org/10.1007/978-1-0716-2047-2biosensors; SELEX; DNA Aptamers; deoxyribozyme; FRET作者: 付出 時間: 2025-3-23 23:24
978-1-0716-2049-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 松軟 時間: 2025-3-24 04:47
Design of a DNAzymethat should help in the design of efficient DNAzymes and show some experimental methods to test the predictions. The main example is for a 10–23 DNAzyme, but the procedure works as well for the 8–17 DNAzyme family.作者: 密碼 時間: 2025-3-24 07:22 作者: 助記 時間: 2025-3-24 11:59 作者: 庇護 時間: 2025-3-24 16:33
Andrzej Ma?owiecki,Iwona Chomiak-Orsayribozymes, but is particularly important for performing in vitro selections. While the production of single-stranded RNA is straightforward by means of in vitro transcription, the enzymatic production of single-stranded DNA?(ssDNA) on a preparative scale is often difficult. Nevertheless, several me作者: 即席 時間: 2025-3-24 22:05 作者: 發(fā)源 時間: 2025-3-25 01:34 作者: 雜役 時間: 2025-3-25 04:46
Peng Zhang,Xiaoyu Ge,Jochen Renzased on their ability to form and change their three-dimensional structure. Binding of divalent ions to specific binding pockets was found to be important for the global structure and function. Here, we present a protocol that allows counting the number of Mn.-binding sites and to determine their di作者: Fracture 時間: 2025-3-25 09:46
Peng Zhang,Xiaoyu Ge,Jochen Renz obtain well-diffracting single crystals and to solve the phase problem. The absence of structural information impedes the elucidation of the molecular details of biological processes. A particularly intriguing example is the RNA-cleavage catalyzed by the 10–23 deoxyribozyme (DNAzyme). This DNAzyme 作者: idiopathic 時間: 2025-3-25 14:33 作者: 弄污 時間: 2025-3-25 19:00 作者: 支形吊燈 時間: 2025-3-25 21:38
Peng Zhang,Xiaoyu Ge,Jochen Renzlations. However, as DNA or RNA are highly charged molecules, performing such simulations is challenging as to the representation of intramolecular electrostatic interactions and those to solvent molecules and ions. This is particularly true for DNAzymes, where DNA and RNA backbones can come as clos作者: REP 時間: 2025-3-26 01:40 作者: mendacity 時間: 2025-3-26 06:43 作者: phytochemicals 時間: 2025-3-26 11:39
https://doi.org/10.1007/978-3-031-00119-2s, for example, oligonucleotides. In order to be applicable, the oligonucleotide has to be labeled site-specifically with paramagnetic tags, the so-called spin labels. Here, we provide a protocol for spin labeling of long oligonucleotides with nitroxides. In the first step, a short and commercially 作者: metropolitan 時間: 2025-3-26 16:14 作者: Conspiracy 時間: 2025-3-26 17:24 作者: Fresco 時間: 2025-3-26 22:11
Selection of Aptamer for ,-Methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzymeing to the metalation of MPIX. The DNA aptamers for porphyrin molecules usually display different catalytic functions (termed deoxyribozymes or DNAzymes), which can accelerate such chemical reactions. Inspired by this, an affinity chromatography selection approach was designed for identifying a porp作者: 斗爭 時間: 2025-3-27 04:57
PCR Methods for the Generation of Catalytic ssDNAyribozymes, but is particularly important for performing in vitro selections. While the production of single-stranded RNA is straightforward by means of in vitro transcription, the enzymatic production of single-stranded DNA?(ssDNA) on a preparative scale is often difficult. Nevertheless, several me作者: 貪婪地吃 時間: 2025-3-27 08:39 作者: 冥界三河 時間: 2025-3-27 11:25 作者: myocardium 時間: 2025-3-27 13:44 作者: Extemporize 時間: 2025-3-27 18:36 作者: Heretical 時間: 2025-3-28 01:06
Crystallization and Structural Determination of 8–17 DNAzymed significant attention. However, due to their intrinsic difficulties in crystallization and structural determination, only very limited structural information of DNAzymes is available to date. Using co-crystallization with the . Polymerase X (.PolX) protein, we have recently solved a complex struct作者: 四牛在彎曲 時間: 2025-3-28 06:10 作者: 宣稱 時間: 2025-3-28 10:04 作者: 斷斷續(xù)續(xù) 時間: 2025-3-28 11:54
Single-Molecule Kinetic Studies of Nucleic Acids by F?rster Resonance Energy Transfer structures and the challenges specific to single-molecule observation techniques, the data recorded in such experiments must be processed via multiple statistical treatments to finally yield a reliable mechanistic view of the NA dynamics. In this chapter, we propose a comprehensive protocol to anal作者: Estrogen 時間: 2025-3-28 18:30 作者: unstable-angina 時間: 2025-3-28 20:46
Spin Labeling of Long RNAs Via Click Reaction and Enzymatic Ligations, for example, oligonucleotides. In order to be applicable, the oligonucleotide has to be labeled site-specifically with paramagnetic tags, the so-called spin labels. Here, we provide a protocol for spin labeling of long oligonucleotides with nitroxides. In the first step, a short and commercially 作者: antenna 時間: 2025-3-28 23:48
Preparation of Site-Specifically Spin-Labeled RNA by in Vitro Transcription Using an Expanded Genetioduction of spin labels at specific positions within the oligonucleotide sequence is a prerequisite. We here present a step-by-step guide to synthesize long RNA oligonucleotides bearing spin labels at specific positions within the sequence. RNA preparation is achieved enzymatically via in vitro tran作者: 虛構(gòu)的東西 時間: 2025-3-29 07:03
A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develoation. Here, we develop a gold nanoparticle (AuNPs)-assisted aptamer selection for the small molecule porphyrin Zinc(.)-Protoporphyrin IX (ZnPPIX), which can realize the selection of DNAzyme without an immobilization step, and the detailed in vitro selection process in solution is described.作者: gangrene 時間: 2025-3-29 09:08 作者: 意外的成功 時間: 2025-3-29 14:12
Andrzej Ma?owiecki,Iwona Chomiak-Orsathods for the production of ssDNA have been published over the years. Here, we present two PCR methods that we find to be particularly effective, fast, and affordable, which we have adapted for our own needs.作者: 微不足道 時間: 2025-3-29 18:45 作者: ACE-inhibitor 時間: 2025-3-29 23:23 作者: 盟軍 時間: 2025-3-30 01:47 作者: hardheaded 時間: 2025-3-30 07:57 作者: DEI 時間: 2025-3-30 11:35 作者: frivolous 時間: 2025-3-30 13:59
Le Zhang,Jagannadan Varadarajan,Yong Peible. The instructions and protocols provided in the following may assist the initial steps of an NMR-based characterization of DNAzymes. To reduce the initial setup requirements and foster exciting new research projects, the discussed approaches focus on experiments that do not require cost-intensive isotope labeling strategies.作者: gruelling 時間: 2025-3-30 19:18 作者: tattle 時間: 2025-3-30 23:35 作者: 南極 時間: 2025-3-31 00:58 作者: Fsh238 時間: 2025-3-31 05:35
Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and Functionble. The instructions and protocols provided in the following may assist the initial steps of an NMR-based characterization of DNAzymes. To reduce the initial setup requirements and foster exciting new research projects, the discussed approaches focus on experiments that do not require cost-intensive isotope labeling strategies.作者: EXULT 時間: 2025-3-31 12:45
Book 2022-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .DNAzymes: Methods and Protocols. is a valuable resource for scientists and researchers interested in learning more about this evolving field.?.
