標(biāo)題: Titlebook: DNA‘Protein Interactions; Principles and Proto Tom Moss Book 20012nd edition Humana Press 2001 DNA.RNA.Termination.Vivo.Western Blotting.ba [打印本頁(yè)] 作者: 可怖 時(shí)間: 2025-3-21 19:01
書(shū)目名稱DNA‘Protein Interactions影響因子(影響力)
作者: 輕彈 時(shí)間: 2025-3-21 23:20 作者: 母豬 時(shí)間: 2025-3-22 02:55 作者: 指耕作 時(shí)間: 2025-3-22 08:03 作者: CARE 時(shí)間: 2025-3-22 09:24 作者: 食物 時(shí)間: 2025-3-22 13:45 作者: 食物 時(shí)間: 2025-3-22 17:25
https://doi.org/10.1007/978-3-031-52407-3living cells, it is often possible to establish the identity of these factors simply by comparison with the consensus binding sites of known factors such as Sp1, AP-1, NF-1, and so forth. The identity of each factor can then be confirmed using in vitro gel shift (electrophoretic mobility shift assay [EMSA]) or footprinting assays.作者: 雄辯 時(shí)間: 2025-3-23 00:53 作者: sundowning 時(shí)間: 2025-3-23 04:40
In Vivo DNA Analysis,living cells, it is often possible to establish the identity of these factors simply by comparison with the consensus binding sites of known factors such as Sp1, AP-1, NF-1, and so forth. The identity of each factor can then be confirmed using in vitro gel shift (electrophoretic mobility shift assay [EMSA]) or footprinting assays.作者: FOVEA 時(shí)間: 2025-3-23 06:35
Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting,troblotting and analysis for sequence-specific DNA binding directly on the blot combines the advantages of a high-resolution fractionation step with the ability to rapidly analyze for a large number of different DNA-binding specificities.作者: Eructation 時(shí)間: 2025-3-23 13:33 作者: Amorous 時(shí)間: 2025-3-23 14:46
Uranyl Photofootprinting, in detail (.,.). It is also widely recognized that uranyl chemistry and photophysics/photochemistry are very complex. Thus monomeric UO..is only present at low pH (pH approx 2), whereas polynuclear species and various “hydroxides,” which often precipitate, form at a higher pH (.).作者: 集中營(yíng) 時(shí)間: 2025-3-23 20:39 作者: 直覺(jué)沒(méi)有 時(shí)間: 2025-3-23 23:05
Learning Motor Control by Dancing YMCAas DNase I or exonuclease III, have the advantage of acting specifically on the DNA. Chemical probes are often less specific and may also react with the protein, possibly disturbing the correct interaction of protein with DNA. For the study of very fragile protein-DNA complexes, enzymatic probes are therefore often preferable.作者: 橫截,橫斷 時(shí)間: 2025-3-24 03:01
Fahed Mostafa,Tharam Dillon,Elizabeth Changcific cis-acting elements, the breakthrough in understanding the molecular basis for the differential activity of these elements has come from the analysis of their recognition by specific DNA-binding proteins.作者: 做方舟 時(shí)間: 2025-3-24 10:18 作者: 小臼 時(shí)間: 2025-3-24 14:05 作者: mendacity 時(shí)間: 2025-3-24 16:46 作者: 前面 時(shí)間: 2025-3-24 19:30 作者: Androgen 時(shí)間: 2025-3-25 00:15 作者: Hectic 時(shí)間: 2025-3-25 03:31 作者: 輪流 時(shí)間: 2025-3-25 10:38
Ethylation Interference,s between macromolecules that juxtapose the specific recognition elements. As part of these processes, proteins make a large number of contacts to the phosphodiester backbone of DNA, as was predicted from biochemical assays of the ionic strength dependence of DNA binding.作者: 發(fā)起 時(shí)間: 2025-3-25 14:07 作者: 階層 時(shí)間: 2025-3-25 15:59
Martina Lucht,Steffi Domagk,Martin Mohringy useful for qualitative purposes, EMSA has the added advantage of being suitable for quantitative and kinetic analyzes (.). Furthermore, because of its very high sensitivity, EMSA makes it possible to resolve complexes of different protein or DNA stoichiometry (.) and even to detect conformational changes.作者: STENT 時(shí)間: 2025-3-25 20:50
https://doi.org/10.1007/978-3-031-52407-3egative superhelicity in free DNA. Both kinks and localized unwinding can arise transiently during the enzymatic manipulation of DNA by recombinases and by protein complexes involved in the establishment of unwound regions during the initiation of transcription or of DNA replication.作者: macabre 時(shí)間: 2025-3-26 02:40
https://doi.org/10.1007/978-981-16-0771-4teraction. Furthermore, in the presence of a large number of tryptophan and tyrosine residues, a relatively small perturbation in the overall emission spectrum brought about by DNA binding may be masked.作者: white-matter 時(shí)間: 2025-3-26 06:04
Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions,y useful for qualitative purposes, EMSA has the added advantage of being suitable for quantitative and kinetic analyzes (.). Furthermore, because of its very high sensitivity, EMSA makes it possible to resolve complexes of different protein or DNA stoichiometry (.) and even to detect conformational changes.作者: 惡名聲 時(shí)間: 2025-3-26 10:26
Diffusible Singlet Oxygen as a Probe of DNA Deformation,egative superhelicity in free DNA. Both kinks and localized unwinding can arise transiently during the enzymatic manipulation of DNA by recombinases and by protein complexes involved in the establishment of unwound regions during the initiation of transcription or of DNA replication.作者: 撕裂皮肉 時(shí)間: 2025-3-26 15:46 作者: thrombosis 時(shí)間: 2025-3-26 18:07
Stefania Montani,Giorgio Leonardictions (.), before being introduced to DNA-protein interaction studies by Jones and Berg in 1966 (.). The principle of the technique is straightforward. Under a wide range of buffer conditions, nucleic acids pass freely through membrane filters, whereas proteins and their bound ligands are retained.作者: Forehead-Lift 時(shí)間: 2025-3-26 20:58
Martina Lucht,Steffi Domagk,Martin Mohringhift or gel retardation assay), first described almost two decades ago (.,.), provides a simple, efficient and widely used method to study such interactions. Its ease of use, its versatility, and especially its high sensitivity (10. mol of DNA [.]) make it a powerful method that has been successfull作者: Leisureliness 時(shí)間: 2025-3-27 04:37
Learning Motor Control by Dancing YMCAnique, a suitable uniquely end-labeled DNA fragment is allowed to interact with a given DNA-binding protein and then the complex partially digested with DNase I. The bound protein protects the region of the DNA with which it interacts from attack by the DNase. Subsequent molecular-weight analysis of作者: MUTED 時(shí)間: 2025-3-27 08:39 作者: Hot-Flash 時(shí)間: 2025-3-27 09:40 作者: sed-rate 時(shí)間: 2025-3-27 15:14
Frederic Stahl,Max Bramer,Mo Addah are discussed elsewhere in the present work. Most footprinting techniques are based on the simple premise of specific DNA regions being protected from the reagent by the bound protein or molecule of interest. However, a number of studies over the past decade have revealed remarkable distortion of 作者: 消息靈通 時(shí)間: 2025-3-27 19:57 作者: A簡(jiǎn)潔的 時(shí)間: 2025-3-27 22:55
CCF-Based Awareness in Agent Model ABGP the photochemically excited state of this ion is a very strong oxidant (.). For instance, uranyl-mediated photooxidation of alcohols has been studied in detail (.,.). It is also widely recognized that uranyl chemistry and photophysics/photochemistry are very complex. Thus monomeric UO..is only pres作者: packet 時(shí)間: 2025-3-28 04:22 作者: 察覺(jué) 時(shí)間: 2025-3-28 08:58 作者: 車(chē)床 時(shí)間: 2025-3-28 10:25 作者: Loathe 時(shí)間: 2025-3-28 16:01 作者: 他去就結(jié)束 時(shí)間: 2025-3-28 21:45 作者: commodity 時(shí)間: 2025-3-29 00:01
Synthesis Lectures on Mechanical Engineeringor in vitro (methylation protection, methylation interference) and in vivo (DMS genomic footprinting) applications because it rapidly reacts with DNA at room temperature and readily penetrates intact cells (.). DMS methylates predominantly the 7-nitrogen of guanine and the 3-nitrogen of adenine. Thu作者: Concrete 時(shí)間: 2025-3-29 04:48
Artificial Intelligence in the Gulfther directly by the formation of hydrogen bonds to base-pair edges from amino acid side chains located on a DNA-binding motif, such as a helix-turnhelix, or indirectly as a result of sequence-dependent distortions of the DNA conformation (.). These contacts occur in the context of oriented complexe作者: Ingest 時(shí)間: 2025-3-29 10:23 作者: 折磨 時(shí)間: 2025-3-29 15:03 作者: 持續(xù) 時(shí)間: 2025-3-29 19:21
https://doi.org/10.1007/978-981-16-0771-4insic fluorescence of the protein arising from the aromatic amino acids, which is frequently perturbed in a DNA-protein complex (..). In some cases, however, changes in the intrinsic fluorescence emission of a protein arising from its interaction with nucleic acid may not be detectable. For example,作者: 相同 時(shí)間: 2025-3-29 20:48
https://doi.org/10.1007/978-981-16-0771-4ses such as DNA replication, transcription, recombination, and repair (.,.). Thus, an understanding of the organization of protein-DNA interactions and associations within the chromatin complex is a prerequisite for a complete molecular description of these processes. For example, the linker histone作者: 通知 時(shí)間: 2025-3-30 01:55
https://doi.org/10.1385/1592592082DNA; RNA; Termination; Vivo; Western Blotting; bacteria; cell; development; gene; genes; nucleic acid; protein; 作者: PANEL 時(shí)間: 2025-3-30 07:51 作者: Outmoded 時(shí)間: 2025-3-30 10:26
CCF-Based Awareness in Agent Model ABGP(.,.). The ability of osmium tetroxide to modify DNA is very sensitive to DNA conformation. In particular, osmium tetroxide will attack thymidines that are unstacked, either because they are in a single-stranded region or for some other reason (e.g., because the DNA is bent or because it is overwound [.]).作者: adjacent 時(shí)間: 2025-3-30 14:18 作者: Adrenal-Glands 時(shí)間: 2025-3-30 18:32 作者: 血友病 時(shí)間: 2025-3-31 00:34
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260230.jpg作者: 影響 時(shí)間: 2025-3-31 01:12
Filter-Binding Assays,binding equilibria and kinetic behavior, and if the DNA samples retained on the filter and in the filtrate are recovered for further processing, the details of the specific binding site can be probed by interference techniques.作者: 閃光你我 時(shí)間: 2025-3-31 08:37
DNase I Footprinting,ng agents (.. and . on the use of hydroxy radicals and diethylpyrocarbonate), its attack on the DNA is relatively easily sterically hindered. Thus, DNase I footprinting is the most likely of all the footprinting techniques to detect a specific DNA-protein interaction. This is clearly demonstrated by作者: photopsia 時(shí)間: 2025-3-31 10:50 作者: 刪減 時(shí)間: 2025-3-31 13:42
Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto South(s) of transcription factor binding within the DNA (size and location of nucleotide stretches or atoms on individual bases) or the complexity of the binding pattern (stoichiometry), but do not yield information about the protein(s) involved. On the other hand, the SW assay reveals the relative molec作者: Tremor 時(shí)間: 2025-3-31 20:12
Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates,inal tails are most likely modulated by the multiple posttranslational phosphorylation events known to occur within these domains (.,.). Thus, the linker histone tails represent critical points for signal transduction within the chromatin complex likely to be manifested as structural alterations wit作者: 碎石 時(shí)間: 2025-4-1 00:17 作者: 意外 時(shí)間: 2025-4-1 04:33 作者: Mortar 時(shí)間: 2025-4-1 07:31
Learning Motor Control by Dancing YMCAng agents (.. and . on the use of hydroxy radicals and diethylpyrocarbonate), its attack on the DNA is relatively easily sterically hindered. Thus, DNase I footprinting is the most likely of all the footprinting techniques to detect a specific DNA-protein interaction. This is clearly demonstrated by作者: 嚴(yán)重傷害 時(shí)間: 2025-4-1 10:22
