標(biāo)題: Titlebook: DNA Vaccines; W. Mark Saltzman,Hong Shen,Janet L. Brandsma Book 2006 Humana Press 2006 Antigen.T cell.autoimmunity.cell.molecular biology. [打印本頁(yè)] 作者: 深謀遠(yuǎn)慮 時(shí)間: 2025-3-21 16:34
書(shū)目名稱DNA Vaccines影響因子(影響力)
作者: semiskilled 時(shí)間: 2025-3-22 00:19
Vaccination With Messenger RNA genome, no induction of autoantibodies. Moreover, mRNA which are generated by in vitro transcription, are easy to produce in large amounts and very high purity. This feature facilitates the good manufacturing practices process and guaranties batch-to-batch reproducibility. Vaccination can be achiev作者: gait-cycle 時(shí)間: 2025-3-22 01:39
A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines-encoding sequences cloned under heterologous promoter control are delivered by techniques that lead in vivo to antigen expression in transfected cells. DNA vaccination efficiently primes both humoral and cellular immune responses. We developed a novel expression system for DNA vaccines in which a f作者: Throttle 時(shí)間: 2025-3-22 04:56
In Vitro Assay of Immunostimulatory Activities of Plasmid Vectorsne is a plasmid DNA vector that can be taken up by cells to produce a protein, encoded by the vector, to be targeted for the induction of humoral or cellular responses. Although the intracellular production of the antigen may promote responses, the vectors themselves may display adjuvant activity be作者: Atrium 時(shí)間: 2025-3-22 10:04 作者: Calculus 時(shí)間: 2025-3-22 14:56 作者: Calculus 時(shí)間: 2025-3-22 17:34
Needle-Free Delivery of Veterinary DNA Vaccinesions of DNA and multiple doses are required before an effective immune response is detected. To overcome these impediments we have developed approaches to deliver the plasmids via needle-free methods, which have been shown to be more effective than traditional needle and syringe methods. Furthermore作者: 展覽 時(shí)間: 2025-3-22 21:52 作者: 鍍金 時(shí)間: 2025-3-23 01:42
A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Nonviral Hybrid Approach DNA molecules are true polymers. Surprisingly, DNA molecules have not been used as a delivery vector material. This is probably due to the fact that almost all DNA have only two shapes: linear or circular. This chapter details our efforts in fabricating highly branched dendrimer-like DNA (DL-DNA) t作者: Noisome 時(shí)間: 2025-3-23 06:06 作者: 裝入膠囊 時(shí)間: 2025-3-23 12:10 作者: 繞著哥哥問(wèn) 時(shí)間: 2025-3-23 14:22
Complexes of DNA Vaccines With Cationic, Antigenic Peptides Are Potent, Polyvalent CD8+ T-Cell-Stimuity. We developed a novel vaccination strategy that used synthetic, cationic (positively charged), and antigenic peptides complexed to negatively charged nucleic acids: antigenic, major histocompatibility complex-class I-binding epitopes fused with a cationic sequence derived from the HIV tat protei作者: 檢查 時(shí)間: 2025-3-23 21:55 作者: 微粒 時(shí)間: 2025-3-23 22:32
Modifying Professional Antigen-Presenting Cells to Enhance DNA Vaccine Potencyssional antigen-presenting cells (APCs), such as dendritic cells (DCs) play important roles in generating humoral and cell-mediated antigen-specific immune responses by DNA vaccination. Continuing progress in our understanding of how professional APCs uptake DNA, process and present the antigens enc作者: 有節(jié)制 時(shí)間: 2025-3-24 02:23
Replicase-Based DNA Vaccines for Allergy Treatmentnd low stability. At ultralow doses, these alphavirus-derived vectors elicit cellular as well as humoral immune responses. Additionally, replicase-based vectors induce “self-removal” of the vaccine via apoptosis of transfected cells. This chapter describes the construction of a replicon-based DNA va作者: Paradox 時(shí)間: 2025-3-24 06:42 作者: grovel 時(shí)間: 2025-3-24 11:49 作者: Dissonance 時(shí)間: 2025-3-24 18:16 作者: A簡(jiǎn)潔的 時(shí)間: 2025-3-24 20:34 作者: 固執(zhí)點(diǎn)好 時(shí)間: 2025-3-24 23:10
Boris Kryzhanovsky,Vladimir Kryzhanovsky-encoding sequences cloned under heterologous promoter control are delivered by techniques that lead in vivo to antigen expression in transfected cells. DNA vaccination efficiently primes both humoral and cellular immune responses. We developed a novel expression system for DNA vaccines in which a f作者: indecipherable 時(shí)間: 2025-3-25 06:22
Hindi Phoneme Recognition - A Reviewne is a plasmid DNA vector that can be taken up by cells to produce a protein, encoded by the vector, to be targeted for the induction of humoral or cellular responses. Although the intracellular production of the antigen may promote responses, the vectors themselves may display adjuvant activity be作者: HILAR 時(shí)間: 2025-3-25 09:36 作者: Kidney-Failure 時(shí)間: 2025-3-25 12:14
Aadil Gani Ganie,Samad Dadvandipoure-sticks, and the need for effective mass immunization. Naked DNA vaccines, as attractive and universal as they appear, have not produced robust immune responses in test systems. However, proof of principle for DNA vaccines has been validated with a number of vaccine candidates in a variety of test 作者: conduct 時(shí)間: 2025-3-25 18:58
https://doi.org/10.1007/978-3-030-82322-1ions of DNA and multiple doses are required before an effective immune response is detected. To overcome these impediments we have developed approaches to deliver the plasmids via needle-free methods, which have been shown to be more effective than traditional needle and syringe methods. Furthermore作者: visceral-fat 時(shí)間: 2025-3-25 23:44 作者: GROVE 時(shí)間: 2025-3-26 01:03 作者: 逢迎白雪 時(shí)間: 2025-3-26 06:15
Alan Bundy,Kwabena Nuamah,Christopher Lucasust enter the cell, navigate its way through endocytic compartments, and ultimately reach the nucleus. Currently, the precise pathway taken by plasmid DNA is not clear. Understanding how plasmid DNA interacts with the cell and which path it follows to reach the nucleus will aid in the rational desig作者: 誹謗 時(shí)間: 2025-3-26 12:04
Francisco Botana,Zoltán Kovács,Tomás Reciothe Toll-like receptor 9 expressed by B cells and plasmacytoid dendritic cells, and trigger the activation of the innate and adaptive immune system. Upon signaling, CpG DNA induces B cells, natural killer cells, macrophages, and dendritic cells to proliferate, differentiate, take up, and present ant作者: BLOT 時(shí)間: 2025-3-26 13:49
Abdulsadek Hassan,Mohammed Angawiity. We developed a novel vaccination strategy that used synthetic, cationic (positively charged), and antigenic peptides complexed to negatively charged nucleic acids: antigenic, major histocompatibility complex-class I-binding epitopes fused with a cationic sequence derived from the HIV tat protei作者: embolus 時(shí)間: 2025-3-26 17:48
Boris Kovalerchuk,Kawa Nazemi,Ebad Banissiox virus (rFPV) vaccines are promising HIV-1 vaccine candidates, although delivering either vaccine alone may be insufficient to induce sufficient T-cell responses. A consecutive immunization strategy, known as “prime-boost,” involving priming with DNA and boosting with rFPV vaccines encoding multip作者: Feedback 時(shí)間: 2025-3-26 21:42
Boris Kovalerchuk,Kawa Nazemi,Ebad Banississional antigen-presenting cells (APCs), such as dendritic cells (DCs) play important roles in generating humoral and cell-mediated antigen-specific immune responses by DNA vaccination. Continuing progress in our understanding of how professional APCs uptake DNA, process and present the antigens enc作者: 抓住他投降 時(shí)間: 2025-3-27 01:23 作者: ABOUT 時(shí)間: 2025-3-27 07:17
DNA Vaccines978-1-59745-168-0Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: anthropologist 時(shí)間: 2025-3-27 10:19 作者: Pericarditis 時(shí)間: 2025-3-27 14:03
DNA Vaccine DesignThe purpose of this chapter is to present basic strategies for the construction of DNA vaccines. This chapter discusses considerations relevant to the selection of a target gene, construction of a DNA expression vector for use as a vaccine, and molecular modifications of the vector to improve protein expression and to augment immunogenicity.作者: 造反,叛亂 時(shí)間: 2025-3-27 21:49
A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines unstable or toxic proteins (or protein domains) can be expressed. We describe the design of DNA vaccines expressing antigens with a stress protein-capturing domain and characterize the immunogenicity of the antigens produced by this expression system.作者: ARM 時(shí)間: 2025-3-27 22:53
Needle-Free Injection of DNA Vaccines: A Brief Overview and Methodologyhancing DNA vaccines. This approach requires no additional development, and with an expanding market and willingness from jet injector manufacturers to produce prefilled syringes, the technique should become feasible for larger phase II/phase III trials.作者: 壓倒性勝利 時(shí)間: 2025-3-28 03:17 作者: MORPH 時(shí)間: 2025-3-28 06:59
Modifying Professional Antigen-Presenting Cells to Enhance DNA Vaccine Potencyegies to increase the numbers of antigen-expressing DCs, strategies to enhance MHC class I and/or II presentation of the encoded antigen and strategies to prolong the life of antigen-expressing DCs to enhance DNA vaccine potency. We will also discuss the methodology involved in DNA vaccine developme作者: 加劇 時(shí)間: 2025-3-28 14:07
1543-1894 ne technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consi978-1-61737-596-5978-1-59745-168-0Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: 發(fā)源 時(shí)間: 2025-3-28 16:43 作者: antidote 時(shí)間: 2025-3-28 19:25
Aadil Gani Ganie,Samad Dadvandipourhancing DNA vaccines. This approach requires no additional development, and with an expanding market and willingness from jet injector manufacturers to produce prefilled syringes, the technique should become feasible for larger phase II/phase III trials.作者: HAVOC 時(shí)間: 2025-3-29 02:16 作者: DOLT 時(shí)間: 2025-3-29 03:09
Boris Kovalerchuk,Kawa Nazemi,Ebad Banissiegies to increase the numbers of antigen-expressing DCs, strategies to enhance MHC class I and/or II presentation of the encoded antigen and strategies to prolong the life of antigen-expressing DCs to enhance DNA vaccine potency. We will also discuss the methodology involved in DNA vaccine developme作者: SOW 時(shí)間: 2025-3-29 08:10
Book 2006tion of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consi作者: Resection 時(shí)間: 2025-3-29 14:39 作者: Lobotomy 時(shí)間: 2025-3-29 16:04 作者: 極少 時(shí)間: 2025-3-29 20:24
Alan Bundy,Kwabena Nuamah,Christopher Lucasmunofluorescence. Together with labeled delivery vectors and/or plasmid DNA, these methods can aid in the understanding of the trafficking pathways involved in DNA delivery, and contribute to the rational design of more efficient delivery vectors.作者: 緩解 時(shí)間: 2025-3-30 01:17 作者: 少量 時(shí)間: 2025-3-30 04:44
Vaccination With Messenger RNAng evaluated in human clinical trials: (1) in vitro mRNA-transfection of dendritic cells to be adoptively transferred and (2) direct injection of globin-stabilized mRNA. This chapter describes the production of mRNA and the preparation of the two types of mRNA-based vaccines tested in humans.作者: 虛構(gòu)的東西 時(shí)間: 2025-3-30 08:31
In Vitro Assay of Immunostimulatory Activities of Plasmid Vectorsand trigger a broad range of pro-inflammatory responses. Because this stimulation results from common sequence motifs, the activity of a plasmid vector can be assessed by the in vitro assay of a limited number of responses, including proliferation of B cells as well as production of cytokines by macrophages or dendritic cells.作者: 引起痛苦 時(shí)間: 2025-3-30 15:27
Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence. Together with labeled delivery vectors and/or plasmid DNA, these methods can aid in the understanding of the trafficking pathways involved in DNA delivery, and contribute to the rational design of more efficient delivery vectors.作者: 進(jìn)入 時(shí)間: 2025-3-30 18:17
Adjuvant Properties of CpG Oligonucleotides in Primatess indicate that CpG oligonucleotides (ODN) are well tolerated and improve the immune response to microbial vaccines. This work examines the progress in utilizing CpG ODN as adjuvants in conventional and DNA vaccines.作者: Dislocation 時(shí)間: 2025-3-31 00:36
Aadil Gani Ganie,Samad Dadvandipours the most commonly used vehicle formulations use materials selected for their general chemical inertness. This chapter describes methods for overcoming this, enabling encapsulation of DNA within degradable microspheres made of a commonly used biomaterial and then covalently conjugating ligands to the surface of these microspheres.作者: VEST 時(shí)間: 2025-3-31 04:12
Abdulsadek Hassan,Mohammed Angawijection of these complexes efficiently primed long-lasting, specific CD8. T-cell immunity of high magnitude. This chapter describes a novel strategy to codeliver complexes of cationic/antigenic peptides bound to antigen-encoding plasmid DNA vaccines in a way that enhances the immunogenicity of both components for T cells.作者: BAIL 時(shí)間: 2025-3-31 06:44 作者: 投射 時(shí)間: 2025-3-31 12:50 作者: 抱狗不敢前 時(shí)間: 2025-3-31 15:17
Surface-Modified Biodegradable Microspheres for DNA Vaccine Deliverys the most commonly used vehicle formulations use materials selected for their general chemical inertness. This chapter describes methods for overcoming this, enabling encapsulation of DNA within degradable microspheres made of a commonly used biomaterial and then covalently conjugating ligands to the surface of these microspheres.
