標(biāo)題: Titlebook: DNA Sequencing Protocols; Hugh G. Griffin,Annette M. Griffin Book 19931st edition Springer Science+Business Media New York 1993 [打印本頁(yè)] 作者: Precise 時(shí)間: 2025-3-21 18:52
書(shū)目名稱(chēng)DNA Sequencing Protocols影響因子(影響力)
作者: 啜泣 時(shí)間: 2025-3-21 20:17 作者: certitude 時(shí)間: 2025-3-22 00:57 作者: Detonate 時(shí)間: 2025-3-22 05:08
K. Bennaceur,Z. Sahraoui,M. A. Nacersis of the labeled strands can easily be synthesized in automatic synthesizers or by hand, the latter of which is more laborious. Various so-called "universal sequencing" and "reverse sequencing" primers, complementary to the beginning of the polylinker region in M13 . cloning phages and plasmids, a作者: 仲裁者 時(shí)間: 2025-3-22 10:26 作者: interlude 時(shí)間: 2025-3-22 16:23 作者: interlude 時(shí)間: 2025-3-22 18:21 作者: Default 時(shí)間: 2025-3-22 21:47 作者: lacrimal-gland 時(shí)間: 2025-3-23 02:52 作者: Pantry 時(shí)間: 2025-3-23 07:02
Transfection of E. coli with M13 DNA,ficiency in some or all strains of . that have been tested. The mechanisms involved in DNA transformation of cells are not fully understood and even with the most efficient methods that are available the proportion of cells that become “competent” for transformation is limited to around 10% of the t作者: ciliary-body 時(shí)間: 2025-3-23 13:03
Sequential Deletions of Single-Stranded DNA,nd complementary to the homopolymer tail, is reannealed across the restriction site and the homopolymer tail. After ligation the DNA is used to transform competent cells and the resulting plaques picked at random for screening.作者: 象形文字 時(shí)間: 2025-3-23 14:33 作者: 事物的方面 時(shí)間: 2025-3-23 20:30
Dideoxy Sequencing Reactions Using T7 Polymerase,the infrequent but specific termination by a ddNTP. This leads to a mixture of DNA fragments of varying lengths that all possess the same 5‘-end owing to the common primer. Since one of the dNTPs contains a radioactive isotope (either .P or .S) these fragments become radioactively labeled and can be作者: 煞費(fèi)苦心 時(shí)間: 2025-3-24 00:13
Dideoxy Sequencing Reactions Using Taq Polymerase,Biochemicals, Cleveland, OH) and the . multiwell. (Amersham, Arlington Heights, IL) kits, both of which gave comparably good results. However these kits are relatively expensive and similar results can be obtained by preparing the different solutions with little loss of time. Here we describe a prot作者: infringe 時(shí)間: 2025-3-24 03:31 作者: FLOUR 時(shí)間: 2025-3-24 09:40 作者: Increment 時(shí)間: 2025-3-24 12:07 作者: 阻撓 時(shí)間: 2025-3-24 18:04
Andrés Iglesias,Akemi Gálvez,Marta Collantessingle-stranded DNAmolecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on l-m long gels (.).作者: 有常識(shí) 時(shí)間: 2025-3-24 21:03
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260196.jpg作者: cogitate 時(shí)間: 2025-3-25 00:42
Cloning into M13,ation method of sequencing DNA (.,.). General aspects of bacteriophage M13 as a cloning vector system are reviewed in ., and the preparation of foreign DNA fragments for M13 cloning is described in .. In this chapter the preparation of M13 vectors and the ligation of foreign DNA fragments (inserts) into M13 vectors are described.作者: 間接 時(shí)間: 2025-3-25 04:39 作者: 閑蕩 時(shí)間: 2025-3-25 10:02
Electrophoresis of Sequence Reaction Samples,single-stranded DNAmolecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on l-m long gels (.).作者: Cholesterol 時(shí)間: 2025-3-25 12:11
David Muhr,Michael Affenzeller,Josef KüngHistorically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(.. This enzyme was derived from E. .. DNA Pol I which, in addition to polymerase activity, has both 3′–5′ and 5′–3′ exonuclease activities.作者: 悲觀 時(shí)間: 2025-3-25 17:11
Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1,Historically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(.. This enzyme was derived from E. .. DNA Pol I which, in addition to polymerase activity, has both 3′–5′ and 5′–3′ exonuclease activities.作者: concert 時(shí)間: 2025-3-25 20:53 作者: 合法 時(shí)間: 2025-3-26 03:35
Panagiota Kotoula,Christos Makrisf DNA in . by cloning vehicles derived from M13mp or pUC made expensive physical separation techniques like ultracentrifugation unnecessary. Although today the polymerase chain reaction is a valuable alternative for the amplification of small DNA pieces (.), it cannot substitute for the construction作者: 戲法 時(shí)間: 2025-3-26 05:59 作者: Debrief 時(shí)間: 2025-3-26 12:11
https://doi.org/10.1007/978-3-319-92016-0e are based on the findings of Mandel and Higa (.) who demonstrated that incubation of cells with naked bacteriophage λ DNA in cold calcium chloride resulted in uptake of virus and that a transient heat-shock of the mixture greatly enhanced the efficiency of transfection. Subsequently, this method w作者: 推測(cè) 時(shí)間: 2025-3-26 16:24 作者: LUT 時(shí)間: 2025-3-26 18:51 作者: nonsensical 時(shí)間: 2025-3-26 22:38 作者: Hippocampus 時(shí)間: 2025-3-27 02:04 作者: pacifist 時(shí)間: 2025-3-27 07:40
https://doi.org/10.1007/978-3-031-08333-4ert for digestion and to protect the primer site. The “Cyclone Sequencing” technique of Dale, McClare, and Houchins (.) overcomes this problem as it is totally independent of the restriction sites in the insert. In this procedure single-stranded Ml3 is hybridized to an oligomer that spans the .dIII 作者: 使苦惱 時(shí)間: 2025-3-27 09:34 作者: 多骨 時(shí)間: 2025-3-27 13:52
K. Bennaceur,Z. Sahraoui,M. A. Nacerherefore, it became the method of choice to obtain several hundred bases of sequence information per reaction. The procedure is based on the enzymatic elongation and radioactive labeling of oligonucleotides that are complementary to the beginning of the single-stranded DNA template. Chain extension 作者: curriculum 時(shí)間: 2025-3-27 17:46 作者: 訓(xùn)誡 時(shí)間: 2025-3-28 01:14
Sesan Akintade,Seongtae Kim,Kaushik Royng a DNA polymerase (.). Several different enzymes are available for this purpose each having different qualities and properties. The use of the Klenow fragment of DNA polymerase I, DNA polymerase, and T7 DNA polymerase in DNA sequence analysis is described elsewhere in this volume.作者: WAX 時(shí)間: 2025-3-28 06:02 作者: 看法等 時(shí)間: 2025-3-28 07:32 作者: figment 時(shí)間: 2025-3-28 13:19 作者: accordance 時(shí)間: 2025-3-28 17:43 作者: 浪費(fèi)物質(zhì) 時(shí)間: 2025-3-28 19:56
Andrés Iglesias,Akemi Gálvez,Marta Collanteseating the DNA with alkali. Conventionally, use of the alkali denaturation method involves neutralization of the sample by acid treatment followed by ethanol precipitation and recovery of the DNA by centrifugation. The time-consuming process of ethanol precipitation may be avoided by neutralizing th作者: hemophilia 時(shí)間: 2025-3-29 02:03
Springer Science+Business Media New York 1993作者: pester 時(shí)間: 2025-3-29 03:46
M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays,of samples is tedious, however, and much time is spent opening and closing tubes and transferring tubes in and out of microcentrifuges. One hundred small volume phenol extractions and ethanol precipitations tasks even the more dedicated sequencer.作者: landfill 時(shí)間: 2025-3-29 11:10
Generation of Random Fragments by Sonication,d. A high proportion of large-scale sequencing projects use random cloning and sequencing (shotgun sequencing) to produce in excess of 95% of the data, and then employ more directed means to complete it (.–.).作者: 要控制 時(shí)間: 2025-3-29 13:42
Pouring Linear and Buffer-Gradient Sequencing Gels,oresis, that IS, during fixing, drying, and autoradiography. The methods given in this chapter deal only with the “standard“ size sequencing gels, but the principles are the same for larger gels, except that more gel mix will be needed, and different sizes of combs, spacers, and glass plates may be needed作者: FOLD 時(shí)間: 2025-3-29 17:36
Plasmid Sequencing, can also serve as satisfactory template DNA. It is particularly important when preparing plasmid DNA for sequencing to give the utmost care and attention to the DNA isolation and purification techniques. Most problems that occur with plasmid sequencing are related to poor quality template.作者: DEBT 時(shí)間: 2025-3-29 21:46 作者: landmark 時(shí)間: 2025-3-30 02:11 作者: entitle 時(shí)間: 2025-3-30 05:14 作者: 熱心 時(shí)間: 2025-3-30 11:54 作者: acheon 時(shí)間: 2025-3-30 15:22 作者: 向前變橢圓 時(shí)間: 2025-3-30 20:33
M13 Cloning Vehicles,h and the two DNA strands simultaneously. For this purpose, a bacteriophage like M 13 can be used. The various viral . and . functions are critical not only for strand separation, but also to separate the single-stranded DNA from the . cell by an active transport mechanism through the intact cell wall.作者: 使痛苦 時(shí)間: 2025-3-30 21:07
Plasmid Sequencing,additional advantage of cleaning-up the template by removing traces of low molecular weight compounds that may interfere with the sequencing reactions. The method used here to prepare plasmid is a variation on the boiled-lysis method (.) using a rapid approach avoiding phenol extraction.作者: 浮雕寶石 時(shí)間: 2025-3-31 03:41
M13 Phage Growth and Single-Strand DNA Preparation,f . (.). Well-separated plaques contain cells infected with phages derived from a single transformation event and these can be picked and regrown to provide pure stocks of recombinant phage particles and DNA.作者: 束以馬具 時(shí)間: 2025-3-31 06:02 作者: Embolic-Stroke 時(shí)間: 2025-3-31 13:11 作者: prostatitis 時(shí)間: 2025-3-31 16:10
David Muhr,Michael Affenzeller,Josef Küngoresis, that IS, during fixing, drying, and autoradiography. The methods given in this chapter deal only with the “standard“ size sequencing gels, but the principles are the same for larger gels, except that more gel mix will be needed, and different sizes of combs, spacers, and glass plates may be needed作者: 熟練 時(shí)間: 2025-3-31 17:38
Arnaud Castelltort,Anne Laurent can also serve as satisfactory template DNA. It is particularly important when preparing plasmid DNA for sequencing to give the utmost care and attention to the DNA isolation and purification techniques. Most problems that occur with plasmid sequencing are related to poor quality template.作者: ORBIT 時(shí)間: 2025-3-31 23:11 作者: 強(qiáng)壯 時(shí)間: 2025-4-1 02:00 作者: 愛(ài)社交 時(shí)間: 2025-4-1 09:21 作者: HUMP 時(shí)間: 2025-4-1 10:27
Subcloning for DNA Sequencing, are very inefficient at completing the last few small sequence gaps that remain near the end of the project. Various nonrandom subcloning methods have also been developed, utilizing partial restriction digests (.), BAL-3 1 digestion (.), exonuclease III digestion (l0–13), and DNase I (.).
