標(biāo)題: Titlebook: cDNA Library Protocols; Ian G. Cowell,Caroline A. Austin Book 1997 Springer Science+Business Media New York 1997 [打印本頁] 作者: 過分愛國主義 時間: 2025-3-21 19:00
書目名稱cDNA Library Protocols影響因子(影響力)
作者: 親密 時間: 2025-3-21 22:29
Applications in Statistical Computingn the λ vector (.). Once a Lambda ZAP library is constructed and amplified, putative clones or the λ library itself may be excised into the phagemid form. Two versions of the excision protocol for Lambda ZAP-based vectors are included here.作者: debble 時間: 2025-3-22 03:45 作者: 華而不實 時間: 2025-3-22 06:30
,Clone Excision Methods for the Lambda ZAP?-Based Vectors,n the λ vector (.). Once a Lambda ZAP library is constructed and amplified, putative clones or the λ library itself may be excised into the phagemid form. Two versions of the excision protocol for Lambda ZAP-based vectors are included here.作者: 饑荒 時間: 2025-3-22 12:11
Review of the Development of Energy Finance cells in a tissue are expressing genes of interest or the tissue is in limited supply. A cDNA library from the targeted cells is preferable to a library constructed from the entire tissue containing these cells, because in the latter case, genes expressed in the targeted cells may be too rare to be作者: OCTO 時間: 2025-3-22 14:58
Sibel Soylu,?lkay ?endeniz-Yüncü,U?ur Soyta?r regionally, temporally, or environmentally specific), a large proportion of all transcripts (approx 40–45%) represent low-abundance mRNAs, present at 1–20 molecules/cell (.). In a given cell type, “l(fā)ow-abundance” mRNAs are likely to represent >95% of the different mRNAs expressed. The lower the ab作者: OCTO 時間: 2025-3-22 18:10
Review of the Development of Energy Financeof RNA can be made from several grams of tissue or as little as 50 mg. However, large samples are generally more representative of the genes expressed in a population of plants in response to environmental cues or at a defined stage of development. Therefore, large-scale extraction of RNA is the met作者: slow-wave-sleep 時間: 2025-3-23 00:53 作者: 大廳 時間: 2025-3-23 04:10 作者: 開始沒有 時間: 2025-3-23 06:13
Wolfgang Gaul,Christoph Winkler The approach described here to clone the missing sequence (cDNA ends) employs polymerase chain reaction (PCR). Since the initial reports of rapid amplification of cDNA ends (RACE) (.) or related techniques (.,.), many labs have developed significant improvements on the basic approach (.–.). The mos作者: peptic-ulcer 時間: 2025-3-23 09:47 作者: 冥想后 時間: 2025-3-23 14:17 作者: 針葉樹 時間: 2025-3-23 18:38 作者: 財產(chǎn) 時間: 2025-3-24 01:49
Applications in Ubiquitous Computingphage λ DNA and produce viable phage particles. The conditions for the transfer of exogenous DNA into . have been examined in detail in studies of bacteriophage transfection, genetic transformation, and plasmid transformation. Modifications that improve the efficiency of transformation include prolo作者: 序曲 時間: 2025-3-24 05:27 作者: folliculitis 時間: 2025-3-24 08:26 作者: infatuation 時間: 2025-3-24 14:26
Applications of Algebraic Topologyly, the obvious approach is to screen a λ-phage expression library using the antibody. In general, polyclonal sera give better results, but a mixture of monoclonal antisera could be used instead. Fortunately, a number of convenient commercial vectors are available that can be used to generate an exp作者: intention 時間: 2025-3-24 15:45 作者: SENT 時間: 2025-3-24 19:03
Lecture Notes in Electrical Engineeringically interacts. Traditionally, this problem has been approached by use of protein biochemistry, involving purification of interacting proteins and determination of at least a portion of their amino acid sequences by microsequencing techniques, so that degenerate oligonucleotides can be prepared an作者: cocoon 時間: 2025-3-25 00:05
Priya Dalal,Gaurav Aggarwal,Sanjay Tejasveeoyed in many laboratories as a means of screening cDNA and genomic fusion libraries for protein interaction partners (.–.). The method relies on the fact that transcription factors, such as the yeast GAL4 factor, consist of separable DNA-binding and transcriptional regulatory domains; the former bei作者: 時代錯誤 時間: 2025-3-25 07:05
https://doi.org/10.1007/978-981-16-3067-5a large number of molecules involved in signaling or adhesion have been cloned, there still remain many unknown molecules that are important in these functions. Most of the molecules involved in signaling or adhesion are secreted or membrane-anchored proteins. Many of these proteins contain a signal作者: 不規(guī)則的跳動 時間: 2025-3-25 09:49
Analysis of Sensors for Movement Analysisormal allele of the corresponding gene. The powerful gene-transfer techniques, which have played a key role in the studies of dominant phenotypes, are not readily applicable to recessive traits, since expression of a recessive allele does not generally affect the cellular phenotype. In haploid organ作者: Mobile 時間: 2025-3-25 14:49
Fintech in Oman: Present and Future Scenarioh recombinant phages (.,.). It offers the advantages of high cloning efficiency, high-level expression, the relative stability of β-galactosidase fusion proteins, and simple approaches to purify the fusion proteins. After the desired clone is detected and purified, it is often necessary to obtain pr作者: Indebted 時間: 2025-3-25 19:33 作者: 叢林 時間: 2025-3-25 21:21 作者: 神化怪物 時間: 2025-3-26 04:05
Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads, technically demanding, time-consuming, and labor-intensive technology, including the need for large amounts of mRNA or highly purified single-stranded DNA. Several subtractive hybridization strategies based on solid-phase hybridization on magnetic Dynabeads have previously been described (.–.). In 作者: exorbitant 時間: 2025-3-26 06:24 作者: 詞匯 時間: 2025-3-26 09:06
,Immunological Screening of λ-Phage cDNA Expression Libraries,gh modern vectors often allow directional cloning. Thus, one in three of the clones to the protein of interest may be recognized by the antibody. Attention should also be given to the average size of the inserts, particularly for a large protein, since even polyclonal sera to the full protein may on作者: 顯微鏡 時間: 2025-3-26 15:04
,Protein Interaction Cloning by Far-Western Screening of λ-Phage cDNA Expression Libraries,y-based screening. This expression cloning approach is based on the “far-western” method (also known as ligand blotting or West-Western) in which proteins immobilized on nitrocellulose or other suitable membranes can be detected by their ability to bind to soluble recombinant proteins of interest (.作者: Glucose 時間: 2025-3-26 17:00
Yeast Two-Hybrid Library Screening, activation domain. Interaction is detected when the reconstituted transcription factor activates a reporter gene (..). DNA-binding domain and activation domain fusion proteins are expressed from plasmid DNAs. The DNA-binding and transcriptional activation components are usually derived from the yea作者: Ossification 時間: 2025-3-26 22:56
Signal Sequence Trap,thod, called Signal Sequence Trap, turns out to be an efficient method to isolate 5′-cDNA fragments from secreted or transmembrane proteins. We have already obtained a number of cDNA clones of putative growth factors, receptors, or adhesion molecules (.–.). Signal Sequence Trap can clone not only se作者: 只有 時間: 2025-3-27 04:54
,Expression and Preparation of Fusion Proteins from Recombinant λgt1.1 Phages,: it is time-consuming, and phage lysogeny occurs at a low frequency. We have previously described a method for making fusion proteins from LB agar plates containing . Y1090 infected with a high concentration of recombinant λgt1.1 phages (up to 5 × 10. PFU/l50 × 15-mm plate) (.). A liquid culture me作者: 性別 時間: 2025-3-27 05:52
Sibel Soylu,?lkay ?endeniz-Yüncü,U?ur Soyta?This is of practical significance in all cases, except where high-abundance mRNAs (accumulating to a few percent of total cellular mRNA) are sought. The implications are particularly severe for Human Genome Project expressed sequence tag (EST) studies, which on the basis of automated sequencing, aim作者: JUST 時間: 2025-3-27 11:42
Applications in Ubiquitous Computing technically demanding, time-consuming, and labor-intensive technology, including the need for large amounts of mRNA or highly purified single-stranded DNA. Several subtractive hybridization strategies based on solid-phase hybridization on magnetic Dynabeads have previously been described (.–.). In 作者: Radiculopathy 時間: 2025-3-27 17:18
Applications of Algebraic Topology + C content, since G:C base pairs possessing three hydrogen bonds interact more strongly than A:T base pairs with two hydrogen bonds. The different oligonucleotides in a mixture will thus possess different melting temperatures. This means that in buffered saline solution, one usually chooses a melt作者: sphincter 時間: 2025-3-27 21:07 作者: engagement 時間: 2025-3-27 23:42
Lecture Notes in Electrical Engineeringy-based screening. This expression cloning approach is based on the “far-western” method (also known as ligand blotting or West-Western) in which proteins immobilized on nitrocellulose or other suitable membranes can be detected by their ability to bind to soluble recombinant proteins of interest (.作者: CHOP 時間: 2025-3-28 02:36
Priya Dalal,Gaurav Aggarwal,Sanjay Tejasvee activation domain. Interaction is detected when the reconstituted transcription factor activates a reporter gene (..). DNA-binding domain and activation domain fusion proteins are expressed from plasmid DNAs. The DNA-binding and transcriptional activation components are usually derived from the yea作者: 準(zhǔn)則 時間: 2025-3-28 07:57
https://doi.org/10.1007/978-981-16-3067-5thod, called Signal Sequence Trap, turns out to be an efficient method to isolate 5′-cDNA fragments from secreted or transmembrane proteins. We have already obtained a number of cDNA clones of putative growth factors, receptors, or adhesion molecules (.–.). Signal Sequence Trap can clone not only se作者: CANDY 時間: 2025-3-28 11:00
Fintech in Oman: Present and Future Scenario: it is time-consuming, and phage lysogeny occurs at a low frequency. We have previously described a method for making fusion proteins from LB agar plates containing . Y1090 infected with a high concentration of recombinant λgt1.1 phages (up to 5 × 10. PFU/l50 × 15-mm plate) (.). A liquid culture me作者: 無畏 時間: 2025-3-28 17:43
Book 1997wer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 1—5 concentrate on cDNA library construction and manipula- tion, Chapters 6 and 7 describe me作者: integral 時間: 2025-3-28 22:47 作者: phase-2-enzyme 時間: 2025-3-28 23:30
1064-3745 se transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con- structin作者: 勉強(qiáng) 時間: 2025-3-29 03:16 作者: 鞭打 時間: 2025-3-29 10:08
Review of the Development of Energy Finance in a population of plants in response to environmental cues or at a defined stage of development. Therefore, large-scale extraction of RNA is the method of choice for preparations to be used for cDNA libraries.作者: 大范圍流行 時間: 2025-3-29 14:39
Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Prihe different members of a gene family carry out related functions. A detailed protocol for the cloning by degenerate oligonucleotide polymerase chain reaction (PCR) of members of the . family of membrane water channels (., .) is discussed here.作者: Meditative 時間: 2025-3-29 18:52
Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli,nged exposure of cells to CaCl. (.), substitution of Ca. ions by other cations, such as Rb. (.), Mn., and K., and addition of other compounds, such as dimethyl sulfoxide (DMSO), dithiothreitol, and cobalt hexaminechloride (.).作者: 巧思 時間: 2025-3-29 20:52
Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide double-stranded DNA probe containing the sequence recognized by the factor in question. Recombinants expressing a protein capable of binding the probe sequence in the presence of nonspecific competitor DNA are thus identified and can be isolated.作者: Emmenagogue 時間: 2025-3-30 00:12
cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR,ary constructed from the entire tissue containing these cells, because in the latter case, genes expressed in the targeted cells may be too rare to be detected. cDNA clones of genes expressed in small amounts of material are often hard to obtain because the construction of conventional cDNA libraries requires microgram amounts of poly A. RNA (.).作者: 船員 時間: 2025-3-30 05:30
Isolation of Messenger RNA from Plant Tissues, in a population of plants in response to environmental cues or at a defined stage of development. Therefore, large-scale extraction of RNA is the method of choice for preparations to be used for cDNA libraries.作者: 閃光你我 時間: 2025-3-30 10:37 作者: 拔出 時間: 2025-3-30 16:23 作者: 果仁 時間: 2025-3-30 16:39
Topological and Algebraic Considerationsdouble-stranded DNA probe containing the sequence recognized by the factor in question. Recombinants expressing a protein capable of binding the probe sequence in the presence of nonspecific competitor DNA are thus identified and can be isolated.作者: 否決 時間: 2025-3-31 00:39
Book 1997iptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con- structing and scre作者: hair-bulb 時間: 2025-3-31 01:44
Applications in Statistical ComputingA fragments, In this case, treatment of the mRNA with a denaturant, such as methyl-mercuric hydroxide, prior to synthesis may be necessary (.). Other potential difficulties include DNA molecules contaminating the mRNA sample. DNA can clone efficiently, and their introns can confuse results. RNase-free DNase treatment of the sample is recommended.作者: 不能逃避 時間: 2025-3-31 05:55 作者: Cardioversion 時間: 2025-3-31 10:05
Pascal Kerschke,Heike Trautmannnd, the intrinsic difficulty of multiple sequential enzymatic reactions required for cDNA cloning often leads to low yields and truncated clones (.). Finally, screening of a library with hybridization technique is time consuming.作者: 爭吵加 時間: 2025-3-31 17:14 作者: Aids209 時間: 2025-3-31 18:43 作者: degradation 時間: 2025-3-31 22:13
,cDNA Library Construction for the Lambda ZAP?-Based Vectors,A fragments, In this case, treatment of the mRNA with a denaturant, such as methyl-mercuric hydroxide, prior to synthesis may be necessary (.). Other potential difficulties include DNA molecules contaminating the mRNA sample. DNA can clone efficiently, and their introns can confuse results. RNase-free DNase treatment of the sample is recommended.作者: 豐富 時間: 2025-4-1 02:17
Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs,E” protocol, which is adapted from the work of a number of laboratories (.–.). Commercial RACE kits are available from Bethesda Research Laboratories (Gaithersburg, MD) (.) and Clontech (Palo Alto, CA) that are convenient, but not as powerful as the most recent versions of classic and new RACE.
