標題: Titlebook: Cytoskeleton; Methods and Protocol Ray H. Gavin Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive li [打印本頁] 作者: 紀念性 時間: 2025-3-21 16:49
書目名稱Cytoskeleton影響因子(影響力)
書目名稱Cytoskeleton影響因子(影響力)學(xué)科排名
書目名稱Cytoskeleton網(wǎng)絡(luò)公開度
書目名稱Cytoskeleton網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Cytoskeleton被引頻次
書目名稱Cytoskeleton被引頻次學(xué)科排名
書目名稱Cytoskeleton年度引用
書目名稱Cytoskeleton年度引用學(xué)科排名
書目名稱Cytoskeleton讀者反饋
書目名稱Cytoskeleton讀者反饋學(xué)科排名
作者: 只有 時間: 2025-3-21 22:12 作者: 智力高 時間: 2025-3-22 03:32
https://doi.org/10.1007/978-3-658-25581-7sional analysis of a yeast cell. As a result, imaging in yeast has emerged as an important tool to study cytoskeletal organization, function, and dynamics. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in live yeast cells.作者: FLAGR 時間: 2025-3-22 06:56
Unternehmensbewertung in der (Corona-)Krise?observed with appropriate visualization techniques without sectioning or dissection. This chapter describes the introduction to representative methods for imaging the actin cytoskeleton in . and a protocol for staining worms with fluorescent phalloidin.作者: Arthropathy 時間: 2025-3-22 09:30
Katrina Koppel,Mari-Liis Jakobson to mimic the local response of the F-actin cytoskeleton to bacteria-generated forces. In this chapter, we describe our magnetic tweezers system and show how to control it in order to study cellular responses to force.作者: padding 時間: 2025-3-22 14:32 作者: padding 時間: 2025-3-22 19:15 作者: obnoxious 時間: 2025-3-22 23:55 作者: DIKE 時間: 2025-3-23 03:08
Imaging the Actin Cytoskeleton in Live Budding Yeast Cellssional analysis of a yeast cell. As a result, imaging in yeast has emerged as an important tool to study cytoskeletal organization, function, and dynamics. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in live yeast cells.作者: Digitalis 時間: 2025-3-23 06:23 作者: Cardiac-Output 時間: 2025-3-23 10:13
Book 2022Latest editionn pitfalls, and step-by-step, readily reproducible protocols..?. .Authoritative and cutting-edge,?.Cytoskeleton: Methods and Protocols, Fourth Edition?.aims to provide an?updated version to?help researchers expand their understanding of cytoskeleton structure and function..作者: sigmoid-colon 時間: 2025-3-23 17:44
Einführung von Standardsoftwarecate in the plane of the plasma membrane and (2) the cortical actin network reorganizes when cells are stimulated to secrete insulin. We describe the FIJI plugins that were used to process and analyze the TIRF-SIM images to obtain quantitative data.作者: 瘙癢 時間: 2025-3-23 19:03 作者: animated 時間: 2025-3-24 01:10
Mari-Liis Jakobson,Russell King,Raivo Vetiko planarian spermatogenesis and current tools for the analysis of molecular players in this process. Step-by-step protocols for isolating and imaging spermatogenic cells are provided with enough detail to be carried out by newcomers to the field who would like to study this unique organism in the laboratory.作者: indubitable 時間: 2025-3-24 05:49
The Poison Panics of British Indiar fast protein liquid chromatography (FPLC) of 6× his-tagged cytoskeletal proteins. The protocol can readily be modified for affinity and non-affinity purification techniques using the various ?KTA? Chromatography Systems.作者: Rct393 時間: 2025-3-24 08:20 作者: IRK 時間: 2025-3-24 12:56
Super-Resolution Imaging of the Actin Cytoskeleton in Living Cells Using TIRF-SIMcate in the plane of the plasma membrane and (2) the cortical actin network reorganizes when cells are stimulated to secrete insulin. We describe the FIJI plugins that were used to process and analyze the TIRF-SIM images to obtain quantitative data.作者: anagen 時間: 2025-3-24 15:13 作者: 手勢 時間: 2025-3-24 22:26
Analysis of Morphogenesis and Flagellar Assembly During Spermatogenesis in Planarian Flatwormso planarian spermatogenesis and current tools for the analysis of molecular players in this process. Step-by-step protocols for isolating and imaging spermatogenic cells are provided with enough detail to be carried out by newcomers to the field who would like to study this unique organism in the laboratory.作者: Brochure 時間: 2025-3-25 01:38
Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC) Using an r fast protein liquid chromatography (FPLC) of 6× his-tagged cytoskeletal proteins. The protocol can readily be modified for affinity and non-affinity purification techniques using the various ?KTA? Chromatography Systems.作者: Visual-Field 時間: 2025-3-25 06:30
Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cellsed to aggregate or disperse throughout the projections. Melanosome migration in RPE apical projections is dependent on actin filaments and thus renders this model system useful for investigations of actin-dependent organelle motility.作者: 投射 時間: 2025-3-25 09:56 作者: Goblet-Cells 時間: 2025-3-25 15:18 作者: 實現(xiàn) 時間: 2025-3-25 15:48 作者: 爆炸 時間: 2025-3-25 23:37
https://doi.org/10.1007/978-3-540-69451-9 available (A, B, C, D, E, H, J), also making it a widely used compound for F-actin disruption. The same can be stated for latrunculins (A, B), derived from Red Sea sponges; however the mode of action is different by binding to G-actin and inhibiting incorporation into the filament. In the case of s作者: Robust 時間: 2025-3-26 04:07
Harald Fischer-Tiné,Christine Whyteudies on cilioprotists and other model organisms that raise specific questions regarding whether basal body- and centriole-associated nucleic acids, both DNA and RNA, should continue to be considered when seeking to employ cilioprotists as model systems for cytoskeletal research; and (3) new, mainly作者: 柔聲地說 時間: 2025-3-26 05:45 作者: 實現(xiàn) 時間: 2025-3-26 11:42
Imaging Cytoskeleton Components by Electron Microscopy) EM (PREM) for visualization of the cytoskeleton. The procedure is applicable to thin cultured cells growing on glass coverslips and consists of detergent extraction (or mechanical “unroofing”) of cells to expose their cytoskeleton, chemical fixation to provide stability, ethanol dehydration and cr作者: 傀儡 時間: 2025-3-26 16:01
Imaging the Cytoskeleton in Living Plant Rootsas ., the polymerized nutrient-supplemented agar is gently lifted or cut and used to secure pre-germinated seeds on the coverslip prior to root imaging. The agar-coverslip system we use for imaging the cytoskeleton in living roots along with general methods for expressing green fluorescent protein (作者: offense 時間: 2025-3-26 18:11 作者: lethargy 時間: 2025-3-27 00:07
The Cilioprotist Cytoskeleton , a Model for Understanding How Cell Architecture and Pattern Are Specudies on cilioprotists and other model organisms that raise specific questions regarding whether basal body- and centriole-associated nucleic acids, both DNA and RNA, should continue to be considered when seeking to employ cilioprotists as model systems for cytoskeletal research; and (3) new, mainly作者: 落葉劑 時間: 2025-3-27 02:00
3D and 4D Tumorigenesis Model for the Quantitative Analysis of Cancer Cell Behavior and Screening fointeractions, cell adhesion molecules, cell surface receptors, and general cell surface markers. The model can also be used for 2D imaging in a 96-well plate for rapid screening and is adaptable for 3D high-resolution assessment. In the latter case, we show how the 3D model can be optically sectione作者: 合法 時間: 2025-3-27 06:29 作者: 微生物 時間: 2025-3-27 12:11 作者: 分離 時間: 2025-3-27 17:28
Imaging the Actin Cytoskeleton in Live Budding Yeast Cellse, rounded morphology, and cell wall. However, with improved techniques, researchers can acquire high-resolution images and carry out rapid multidimensional analysis of a yeast cell. As a result, imaging in yeast has emerged as an important tool to study cytoskeletal organization, function, and dyna作者: 蒙太奇 時間: 2025-3-27 17:50
Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cellsrform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fl作者: 碎石 時間: 2025-3-28 01:34
Imaging of Actin Cytoskeletal Integrity During Aging in integrity of the cytoskeleton is important during aging, as the decline of cytoskeletal integrity contributes to the physiological consequence of aging. Moreover, improving cytoskeletal form and function throughout aging is sufficient to drive life span extension and promote organismal health in mul作者: Heretical 時間: 2025-3-28 03:20 作者: 百科全書 時間: 2025-3-28 09:34 作者: 功多汁水 時間: 2025-3-28 11:46
Imaging of the Cytoskeleton Using Live and Fixed , Tissue Culture Cellsm. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches and high-throughput RNAi screens. Here we describe . S2 cell culture and preparation for live-cell and fixed-cell fluorescen作者: conformity 時間: 2025-3-28 16:00
Influencing the Actin Dynamics in Plant Cells by Jasplakinolide , Chondramides , Phalloidin , Cytoch morphogenesis, motility, organelle movement, apoptosis, or abiotic stress. These substances can be divided into two major subclasses—F-actin-stabilizing and F-actin-polymerizing substances like jasplakinolide and chondramides and F-actin-severing compounds like cytochalasins and latrunculins. Jaspl作者: 公共汽車 時間: 2025-3-28 22:37 作者: JADED 時間: 2025-3-28 23:03
An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Celle developed an in vitro system that combines micromanipulation of force by magnetic tweezers with simultaneous live cell fluorescence microscopy. We applied pulling forces to magnetic beads coated with the . Type IV pili in the same order of magnitude than the forces generated by live bacteria. We s作者: 本土 時間: 2025-3-29 07:02
Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC) Using an a of intracellular processes such as maintenance of cellular organization, organelle translocation, and various nuclear roles including chromosome separation during mitosis. Early methods for protein extraction primarily relied on the salting-out method which was performed in conjunction with bioche作者: 不利 時間: 2025-3-29 09:32
The Cilioprotist Cytoskeleton , a Model for Understanding How Cell Architecture and Pattern Are Spec feeding, reproductive, and sensory behaviors of the multitude of cilioprotist species that inhabit the aquatic environment. In the past 10–20?years, many new discoveries and technologies have helped to advance our understanding of how cytoskeletal organelles are assembled in many different eukaryot作者: PLAYS 時間: 2025-3-29 13:48
3D and 4D Tumorigenesis Model for the Quantitative Analysis of Cancer Cell Behavior and Screening foe is a dynamic process mediated by a subset of cells within the population of cancer cells. In contrast, non-tumorigenic cells from normal cell lines and normal tissues do not aggregate or coalesce, nor do they possess the motile cell types that orchestrate coalescence of cancer cells. Therefore, co作者: orient 時間: 2025-3-29 18:53 作者: 的闡明 時間: 2025-3-29 20:57 作者: IOTA 時間: 2025-3-30 00:58
https://doi.org/10.1007/978-1-0716-1661-1Nanobodies; Immunofluorescence; Reconstitutions; Chemotaxis; Proteomics作者: 阻擋 時間: 2025-3-30 07:13 作者: 訓(xùn)誡 時間: 2025-3-30 10:05
Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cellsrform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fluorescence microscopy.作者: 步履蹣跚 時間: 2025-3-30 15:02
Imaging of the Cytoskeleton Using Live and Fixed , Tissue Culture Cellsm. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches and high-throughput RNAi screens. Here we describe . S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.作者: 纖細 時間: 2025-3-30 20:01 作者: 名字的誤用 時間: 2025-3-30 20:47 作者: Aspirin 時間: 2025-3-31 04:31 作者: 通知 時間: 2025-3-31 05:22
Anwendungsorientierte Wirtschaftsinformatikrform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fl作者: SPECT 時間: 2025-3-31 10:48
Mehr-Ebenen-Betrachtung bei der Gestaltungintegrity of the cytoskeleton is important during aging, as the decline of cytoskeletal integrity contributes to the physiological consequence of aging. Moreover, improving cytoskeletal form and function throughout aging is sufficient to drive life span extension and promote organismal health in mul作者: 拖債 時間: 2025-3-31 16:30 作者: hypnogram 時間: 2025-3-31 19:26 作者: Coterminous 時間: 2025-4-1 00:57
,SDAI—integriertes Archivieren,m. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches and high-throughput RNAi screens. Here we describe . S2 cell culture and preparation for live-cell and fixed-cell fluorescen作者: 晚間 時間: 2025-4-1 04:43
