標(biāo)題: Titlebook: Chromatin Immunoprecipitation; Methods and Protocol Franziska Greulich Book 2024 The Editor(s) (if applicable) and The Author(s), under exc [打印本頁] 作者: 欺侮 時(shí)間: 2025-3-21 19:08
書目名稱Chromatin Immunoprecipitation影響因子(影響力)
作者: FOIL 時(shí)間: 2025-3-21 21:08 作者: aviator 時(shí)間: 2025-3-22 01:26
Chromatin Immunoprecipitation in Adipose Tissue and Adipocytes: How to Proceed and Optimize the Prontrol of specific transcriptional factors or co-regulators at loci-specific or pan-genomic levels..Here we provide a reliable method for processing ChIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipi作者: 自然環(huán)境 時(shí)間: 2025-3-22 07:31 作者: 冰雹 時(shí)間: 2025-3-22 11:20 作者: meritorious 時(shí)間: 2025-3-22 13:09 作者: meritorious 時(shí)間: 2025-3-22 20:02
Sequential ChIP-Seq, assay determines the in vivo co-localization of two proteins to the same genomic locus. In this chapter, we combine the two protocols in Sequential ChIP-Seq, a method for identifying genome-wide sites of in vivo protein co-occupancy.作者: inveigh 時(shí)間: 2025-3-23 00:30 作者: tangle 時(shí)間: 2025-3-23 03:23
Estrogen Receptor Chromatin Profiling by CUT&RUN,f gene transcription. Steroid hormones activate nuclear hormone receptor (HR), transcription factors (TFs), which bind DNA in a tissue- and cell type–specific manner to influence cellular function. Identifying the genomic binding sites of HRs is essential to understanding mechanisms of hormone signa作者: critic 時(shí)間: 2025-3-23 09:25
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) in Macrophages, valuable for the characterization of the binding of transcription factors or co-regulators genome wide. Furthermore, it can be used for epigenetic profiling, chromatin accessibility assessment, and identification of regulatory elements. Compared to the more commonly used chromatin immunoprecipitati作者: 敏捷 時(shí)間: 2025-3-23 12:24 作者: Expertise 時(shí)間: 2025-3-23 14:03
CUT&Tag for Efficient Epigenomic Profiling of Frozen Tissues,col details the steps to generate CUT&Tag libraries from fresh or frozen tissues. This CUT&Tag workflow has nine main steps: isolation of nuclei from tissues, binding of nuclei to Concanavalin A–coated beads, binding of the primary antibody, binding of the secondary antibody, binding pA-Tn5 adapter 作者: 要塞 時(shí)間: 2025-3-23 20:39 作者: Fierce 時(shí)間: 2025-3-23 22:21
Single-Cell Histone Modification Profiling with Cell Enrichment Using sortChIC,tiation, the distribution of histone modifications is remodeled, resulting in cell type–specific patterns. In the past, their study was limited to abundant cell types that could be purified in necessary numbers. However, studying these cell type–specific dynamic changes in heterogeneous in vivo sett作者: DUST 時(shí)間: 2025-3-24 04:26 作者: 燒烤 時(shí)間: 2025-3-24 08:43
ChEC-Seq: A Comprehensive Guide for Scalable and Cost-Efficient Genome-Wide Profiling in ,nding locations in vivo, does not require antibodies or fixation, and provides genome-wide coverage at near nucleotide resolution..The core of this method is an MNase fusion of the target protein, which allows it, when triggered by calcium exposure, to cut DNA at its binding sites and to generate sm作者: 一窩小鳥 時(shí)間: 2025-3-24 14:30
Chromatin Immunoprecipitation in Adipose Tissue and Adipocytes: How to Proceed and Optimize the ProIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipitation, and DNA purification. We also discuss critical steps for optimizing the experiment to perform a successful ChIP in lipid-rich cells/tissues.作者: Mirage 時(shí)間: 2025-3-24 17:07
Bioinformatics Core Workflow for ChIP-Seq Data Analysis,t covers data processing and initial analytical steps of ChIP-seq data. We provide a step-by-step protocol of the commands as well as a fully assembled Snakemake workflow. Along the protocol, we discuss key tool parameters, quality control, output reports, and preliminary results.作者: 良心 時(shí)間: 2025-3-24 22:16 作者: Surgeon 時(shí)間: 2025-3-25 01:39 作者: 到婚嫁年齡 時(shí)間: 2025-3-25 04:52
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) of Epigenetic Regulators, is a higher signal-to-noise ratio and decreased required starting material. This allows for high-fidelity sequence identification from as few as 500 cells, enabling chromatin profiling of precious tissue samples or primary cell types, as well as less abundant chromatin-binding proteins: all at significantly increased throughput.作者: 改良 時(shí)間: 2025-3-25 10:28
Egisto Boschetti,Pier Giorgio Righetticomplex, tagmentation, DNA extraction, PCR, and post-PCR cleanup and size selection. This protocol enabled us to generate and sequence CUT&Tag libraries across a broad range of fresh and frozen tissue types.作者: OREX 時(shí)間: 2025-3-25 12:29 作者: Corroborate 時(shí)間: 2025-3-25 18:11
Stuart R. Gallant,Vish Koppaka,Nick Zecherle depend on an optimal cross-linking procedure. This method may help extract novel gene regulatory circuits involving previously undetectable TFs. The XL-DNase-seq method is illustrated here for activated mouse macrophage-like cells, which share several features with inflammatory macrophages.作者: 夸張 時(shí)間: 2025-3-25 20:41 作者: Aphorism 時(shí)間: 2025-3-26 00:58 作者: 召集 時(shí)間: 2025-3-26 07:31
Matthew A. Roberts,Richard A. Dursted cell amounts that are required for CUT&RUN, which makes it more attractive for experiments with limited cell numbers. In this chapter, we describe a reliable CUT&RUN protocol for macrophages that can be performed within 2?days and includes a library preparation so that the sample can be directly sequenced.作者: 不怕任性 時(shí)間: 2025-3-26 12:08 作者: 憂傷 時(shí)間: 2025-3-26 13:40
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) in Macrophages,ed cell amounts that are required for CUT&RUN, which makes it more attractive for experiments with limited cell numbers. In this chapter, we describe a reliable CUT&RUN protocol for macrophages that can be performed within 2?days and includes a library preparation so that the sample can be directly sequenced.作者: Thyroxine 時(shí)間: 2025-3-26 18:35
1064-3745 experts.This comprehensive guide delves into the protocols and strategies for investigating gene regulation both experimentally and computationally. It focuses on the use of Chromatin Immunoprecipitation (ChIP) coupled with next-generation sequencing, providing a robust framework for profiling DNA-作者: 擁護(hù) 時(shí)間: 2025-3-26 23:31 作者: 不怕任性 時(shí)間: 2025-3-27 02:29 作者: 不利 時(shí)間: 2025-3-27 07:00
Affects in 21st-Century British Theatreion experiments. In these experiments, different conditions are compared to find the underlying biological mechanisms caused by the stimulus or treatment. In addition, we provide a sample analysis using the steps outlined in the chapter.作者: 使害怕 時(shí)間: 2025-3-27 13:31 作者: Dorsal-Kyphosis 時(shí)間: 2025-3-27 14:17 作者: 迅速飛過 時(shí)間: 2025-3-27 21:02 作者: 憤慨一下 時(shí)間: 2025-3-28 00:17 作者: 冰雹 時(shí)間: 2025-3-28 03:50
Integrative Analysis of CUT&Tag and RNA-Seq Data Through Bioinformatics: A Unified Workflow for Enhne modifications, and chromatin accessibility via CUT&Tag is still in its infancy compared to the well-established ChIP-Seq. This chapter describes a robust bioinformatics methodology and workflow to perform an integrative CUT&Tag/RNA-Seq analysis.作者: 語源學(xué) 時(shí)間: 2025-3-28 10:14 作者: conceal 時(shí)間: 2025-3-28 13:40
https://doi.org/10.1007/978-3-319-60669-9k method. While crosslinked ChIP can be used for all kinds of targets, native ChIP is predominantly used for strong and direct DNA interactors like histones and their modifications. Here we describe a native ChIP protocol that can be used for cells and tissue material.作者: Arb853 時(shí)間: 2025-3-28 14:37 作者: Hirsutism 時(shí)間: 2025-3-28 19:51
Pablo Fossa,Cristian Cortés-Riverantrol of specific transcriptional factors or co-regulators at loci-specific or pan-genomic levels..Here we provide a reliable method for processing ChIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipi作者: accomplishment 時(shí)間: 2025-3-28 22:54
https://doi.org/10.1007/978-3-031-31709-5nteractions in the last decade. ChIP-seq technology became standard both experimentally and computationally. This chapter presents a core workflow that covers data processing and initial analytical steps of ChIP-seq data. We provide a step-by-step protocol of the commands as well as a fully assemble作者: stress-test 時(shí)間: 2025-3-29 06:41 作者: 連系 時(shí)間: 2025-3-29 09:49 作者: crucial 時(shí)間: 2025-3-29 13:12 作者: 格言 時(shí)間: 2025-3-29 19:13
https://doi.org/10.1007/978-3-642-79726-2of defined genes. In this study, we developed a novel method for capturing the proteins that bind to certain chromatin fragments or DNA sequences, which is called reverse chromatin immunoprecipitation (R-ChIP). This technology uses a set of specific DNA probes labeled with biotin to isolate chromati作者: 遺傳 時(shí)間: 2025-3-29 20:46 作者: fertilizer 時(shí)間: 2025-3-30 00:09 作者: sundowning 時(shí)間: 2025-3-30 07:25 作者: Hypomania 時(shí)間: 2025-3-30 09:00 作者: Fibrin 時(shí)間: 2025-3-30 14:49
Introduction to Macroporous Cryogelsunoprecipitation (ChIP-Seq), requires only a limited amount of cells as starting material. RNA sequencing (RNA-Seq) reveals the presence and quantity of RNA in a biological sample, describing the continuously changing cellular transcriptome. The integrated analysis of transcriptional activity, histo作者: 騷動(dòng) 時(shí)間: 2025-3-30 17:45
https://doi.org/10.1007/978-1-59745-582-4tiation, the distribution of histone modifications is remodeled, resulting in cell type–specific patterns. In the past, their study was limited to abundant cell types that could be purified in necessary numbers. However, studying these cell type–specific dynamic changes in heterogeneous in vivo sett作者: Irksome 時(shí)間: 2025-3-30 22:45
Stuart R. Gallant,Vish Koppaka,Nick Zecherlese-seq data at the nucleotide level. We introduce an assay termed cross-link (XL)-DNase-seq, designed to capture chromatin interactions of dynamic TFs. A mild cross-linking step in XL-DNase-seq improves the detection of DNase-based footprints of dynamic TFs. The footprint strengths and detectability作者: 喃喃而言 時(shí)間: 2025-3-31 01:30
Adam Charlton,Michael Zachariounding locations in vivo, does not require antibodies or fixation, and provides genome-wide coverage at near nucleotide resolution..The core of this method is an MNase fusion of the target protein, which allows it, when triggered by calcium exposure, to cut DNA at its binding sites and to generate sm作者: EXALT 時(shí)間: 2025-3-31 08:37 作者: HAIRY 時(shí)間: 2025-3-31 11:47
