標(biāo)題: Titlebook: Cancer Cell Dormancy; Methods and Protocol Marco Montagner Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen [打印本頁(yè)] 作者: Taft 時(shí)間: 2025-3-21 16:09
書(shū)目名稱(chēng)Cancer Cell Dormancy影響因子(影響力)
作者: Semblance 時(shí)間: 2025-3-21 22:40 作者: 鈍劍 時(shí)間: 2025-3-22 04:00
https://doi.org/10.1007/978-3-030-48989-2 and continues to provide critical insights into the biology of dormant tumor cells. It also serves as an accessible tool to identify new therapeutic strategies targeting dormancy and concurrently evaluate the efficacy of therapeutic agents as well as their metabolism and dose-limiting toxicity.作者: 支架 時(shí)間: 2025-3-22 07:05 作者: Hangar 時(shí)間: 2025-3-22 11:55
Kinematics of the “Ai-Gerim” Robot Armcycle inhibitor p27 (mVenus-p27K.) that identifies quiescent cells. When implanted in animal models or assembled in cocultures in vitro, labeled cells can be imaged longitudinally over time or retrieved alive alongside their surrounding microenvironment for downstream gene, protein, and metabolite p作者: syring 時(shí)間: 2025-3-22 13:46
https://doi.org/10.1007/978-3-319-33313-7the host tissue in regulating the proliferation of DTCs. However, the details of such interactions remain to be fully delineated. Here I describe the methods for visualizing and analyzing the interactions between DTCs and the extracellular matrix (ECM) components of the host tissue as well as the cy作者: syring 時(shí)間: 2025-3-22 20:21
Clinical Tumor Dormancy,on the view of cancer development as a tissue-level process. We believe that this will help harmonize the molecular biology research with the new conceptual approach and bridge the knowledge gap on dormancy between bench and bedside.作者: 去掉 時(shí)間: 2025-3-22 23:43 作者: OREX 時(shí)間: 2025-3-23 01:39 作者: Alcove 時(shí)間: 2025-3-23 06:22
A Live Tracker of Dormant Disseminated Tumor Cells,cycle inhibitor p27 (mVenus-p27K.) that identifies quiescent cells. When implanted in animal models or assembled in cocultures in vitro, labeled cells can be imaged longitudinally over time or retrieved alive alongside their surrounding microenvironment for downstream gene, protein, and metabolite p作者: Adulate 時(shí)間: 2025-3-23 13:21
Visualizing the Cell-Matrix Interactions and Cytoskeleton of Disseminated Tumor Cells,the host tissue in regulating the proliferation of DTCs. However, the details of such interactions remain to be fully delineated. Here I describe the methods for visualizing and analyzing the interactions between DTCs and the extracellular matrix (ECM) components of the host tissue as well as the cy作者: 慌張 時(shí)間: 2025-3-23 14:14 作者: obsession 時(shí)間: 2025-3-23 18:21
https://doi.org/10.1007/978-1-0716-3882-83D mammary organoids; KISS1; Bioengineered implantable scaffolds; Microscopic Bone Metastasis; Natural K作者: 背帶 時(shí)間: 2025-3-23 23:17
978-1-0716-3884-2The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Nerve-Block 時(shí)間: 2025-3-24 03:32
Characterizing Rare Dormant and Cycling Lineages Using the Watermelon System, deconvolve lineage identity from single-cell RNA sequencing transcriptional data. Here we describe the Watermelon system, which facilitates the simultaneous tracing of lineage, transcriptional, and proliferative state at a single cell level.作者: Maximize 時(shí)間: 2025-3-24 07:15
Cancer Cell Dormancy978-1-0716-3882-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: alabaster 時(shí)間: 2025-3-24 14:03 作者: Grievance 時(shí)間: 2025-3-24 15:43
Marco MontagnerIncludes cutting-edge methods and protocols.Contains key notes and implementation advice from the experts.Provides step-by-step detail essential for reproducible results作者: 斗爭(zhēng) 時(shí)間: 2025-3-24 22:40 作者: Infelicity 時(shí)間: 2025-3-24 23:31 作者: 不法行為 時(shí)間: 2025-3-25 04:30 作者: mighty 時(shí)間: 2025-3-25 07:51 作者: 顛簸下上 時(shí)間: 2025-3-25 14:46
Advances in Service and Industrial Roboticssome survive and enter a state of dormancy and chemoresistance due to their close interaction with cells in the bone marrow hematopoietic niche. Over many years, some of the cells reawaken and result in metastatic disease that cannot be cured. Analyzing the cellular and molecular interactions betwee作者: inflate 時(shí)間: 2025-3-25 17:11 作者: constitute 時(shí)間: 2025-3-25 20:41
Advances in Service and Industrial Roboticspitulates the complex tumor microenvironment. The chicken embryo chorioallantoic membrane (CAM) is an in vivo system suitable for the implantation of xenograft tumor models. It allows the study of different aspects of the metastatic process, including the dormancy-awakening transition. The main adva作者: 是剝皮 時(shí)間: 2025-3-26 00:54
Advances in Service and Industrial Roboticst the need for exogenous supplementation of hormones. The naturally low 17-β-estradiol levels in host mice recapitulate levels seen in post-menopausal women. By introducing breast cancer cells directly into their “natural” microenvironment of the milk ducts, these cells maintain hormone receptor sta作者: GET 時(shí)間: 2025-3-26 08:05 作者: 尊重 時(shí)間: 2025-3-26 10:53 作者: vocation 時(shí)間: 2025-3-26 13:13
Kinematics of the “Ai-Gerim” Robot Armsting cure (Aguirre-Ghiso, Nat Rev Cancer 7:834–846, 2007; Klein, Nat Rev Cancer 20(11):681–694, 2020; Lyden et al. Cancer Cell 40:787–791, 2022). Despite its clinical significance, the study of what drives DTCs in and out of dormancy while they linger in distant sites has been challenged by the lac作者: Control-Group 時(shí)間: 2025-3-26 18:09 作者: Ibd810 時(shí)間: 2025-3-26 21:08 作者: 表否定 時(shí)間: 2025-3-27 04:06 作者: 拘留 時(shí)間: 2025-3-27 06:10
Antonio Celesti,Philipp Leitner tumor cells survive and transition to a proliferative state to generate a metastatic lesion. On the other hand, metabolic rewiring has been shown to influence metastasis development through the modulation of both intracellular signaling and the crosstalk between metastatic cells and their microenvi作者: 個(gè)阿姨勾引你 時(shí)間: 2025-3-27 11:31 作者: 肥料 時(shí)間: 2025-3-27 16:31
https://doi.org/10.1007/978-3-030-00232-9High-throughput transcriptome RNA sequencing is a powerful tool for understanding dynamic biological processes. Here, we present a computational framework, implemented in an R package QDSWorkflow, to characterize heterogeneous cellular dormancy depth using RNA-sequencing data from bulk samples and single cells.作者: 口味 時(shí)間: 2025-3-27 19:20
Modeling the Depth of Cellular Dormancy from RNA-Sequencing Data,High-throughput transcriptome RNA sequencing is a powerful tool for understanding dynamic biological processes. Here, we present a computational framework, implemented in an R package QDSWorkflow, to characterize heterogeneous cellular dormancy depth using RNA-sequencing data from bulk samples and single cells.作者: fructose 時(shí)間: 2025-3-28 00:48 作者: 火海 時(shí)間: 2025-3-28 02:45 作者: FRONT 時(shí)間: 2025-3-28 06:26
Christian Thormann,Alexander Winklerr dormant behavior (D2.0R, MCF7) or transient dormant metastatic behavior (D2A1) at a metastatic secondary site. Additionally, we present an in vitro and complementary in vivo system to study the switch from dormancy to metastatic growth driven by a fibrotic-like milieu enriched with the deposition of type I collagen.作者: BORE 時(shí)間: 2025-3-28 13:40
Advances in Service and Industrial Roboticschniques. This manuscript describes the basic techniques for generating bone marrow stromal monolayers, co-incubating cancer cells and determining the effects on cancer cell proliferation and molecular signaling.作者: Hemoptysis 時(shí)間: 2025-3-28 16:35
Ignacy Duleba,Iwona Karcz-Dulebain vivo effects on the local and systemic immune responses. While originally developed to characterize the effects of methotrexate and doxorubicin on murine 4T1 breast cancer cells and the relative immune response, the method can be broadened to other chemotherapies and syngeneic cancer models.作者: 苦惱 時(shí)間: 2025-3-28 20:06
In Vitro and In Vivo Systems to Study Tumor Dormancy and the Transition to Overt Metastases Inducedr dormant behavior (D2.0R, MCF7) or transient dormant metastatic behavior (D2A1) at a metastatic secondary site. Additionally, we present an in vitro and complementary in vivo system to study the switch from dormancy to metastatic growth driven by a fibrotic-like milieu enriched with the deposition of type I collagen.作者: 冬眠 時(shí)間: 2025-3-28 23:22
Bone Marrow Stroma Co-cultivation Model of Breast Cancer Dormancy,chniques. This manuscript describes the basic techniques for generating bone marrow stromal monolayers, co-incubating cancer cells and determining the effects on cancer cell proliferation and molecular signaling.作者: 一小塊 時(shí)間: 2025-3-29 04:51
Establishing and Characterizing Chemotherapy-Induced Immunological Dormant Tumor Cell Lines,in vivo effects on the local and systemic immune responses. While originally developed to characterize the effects of methotrexate and doxorubicin on murine 4T1 breast cancer cells and the relative immune response, the method can be broadened to other chemotherapies and syngeneic cancer models.作者: 挑剔小責(zé) 時(shí)間: 2025-3-29 10:24
KISS1 and Kisspeptins Detection in Cell Lines, and social behaviors. These diverse functions point to KISS1 as a potential therapeutic molecule. Here we describe a methodology to detect KISS1 and KP from cell lysate and conditioned media in cell lines. This will serve as a critical tool to study KISS1 processing in KP.作者: 痛恨 時(shí)間: 2025-3-29 13:30 作者: iodides 時(shí)間: 2025-3-29 17:49
Antonio Celesti,Philipp Leitnerronment. Thus, studying the metabolic features of dormant disseminated tumor cells has gained importance in understanding the dormancy process. Here, we describe a method to perform metabolomics and .C tracer analysis in 3D cultures of dormant breast cancer cells.作者: reception 時(shí)間: 2025-3-29 20:11
Advances in Service and Industrial Roboticsthe use of GFP/RFP:Luc2 reporters, we can monitor in vivo tumour growth and conduct ex vivo metastases assays to evaluate dormant metastatic cell harboring organs. Upon recovery of metastatic cells from ER. breast cancer models, downstream analyses can be conducted to assess the relationship between epithelial plasticity and metastatic dormancy.作者: 留戀 時(shí)間: 2025-3-30 03:35
https://doi.org/10.1007/978-3-030-00232-9g. In addition, a methodology for recovering brain metastatic cancer cells from the mouse brain is described, providing technical tips for unraveling the mysteries of cancer cell dormancy in brain metastasis.作者: 松馳 時(shí)間: 2025-3-30 04:51
Luiz Angelo Steffenel,Manuele Kirch Pinheirorom cisplatin chemotherapy. Many tools have been developed to monitor ROS production in cells in culture, while our ability to detect this in vivo remains limited. Here we describe a detailed protocol for determination of ROS in metastatic cells in the mouse lung tissue by detecting 4-hydroxy-2-noneal (4HNE) adducts formation in fixed tissues.作者: Consequence 時(shí)間: 2025-3-30 12:08
Preclinical Mouse Intraductal Model (MIND) to Study Metastatic Dormancy in Estrogen Receptor-Positithe use of GFP/RFP:Luc2 reporters, we can monitor in vivo tumour growth and conduct ex vivo metastases assays to evaluate dormant metastatic cell harboring organs. Upon recovery of metastatic cells from ER. breast cancer models, downstream analyses can be conducted to assess the relationship between epithelial plasticity and metastatic dormancy.作者: 脫落 時(shí)間: 2025-3-30 14:47 作者: 上流社會(huì) 時(shí)間: 2025-3-30 19:39 作者: Fulsome 時(shí)間: 2025-3-31 00:24
1064-3745 tial for reproducible results.This volume provides protocols and techniques to study metastatic dormant cells. Chapters focus on in vitro systems to model metastatic dormancy in vitro, detection of disseminated tumor cells in vivo, perturbation of key molecules involved in metastatic dormancy, ?and 作者: EVEN 時(shí)間: 2025-3-31 04:26 作者: obviate 時(shí)間: 2025-3-31 07:27
In Ovo Xenograft Model to Study Tumor Dormancy, Tumor Cell Dissemination and Metastasis,ntages of this system are its high reproducibility, cost-effectiveness, and versatility. Here, by using two dormancy tumor models, one of head and neck squamous cell carcinoma and one of breast cancer, we described a detailed protocol for the use of the CAM model in metastasis assays and for the study of tumor growth and dormancy.作者: 推延 時(shí)間: 2025-3-31 12:36
13C Tracer Analysis and Metabolomics in Dormant Cancer Cells,ronment. Thus, studying the metabolic features of dormant disseminated tumor cells has gained importance in understanding the dormancy process. Here, we describe a method to perform metabolomics and .C tracer analysis in 3D cultures of dormant breast cancer cells.作者: 形狀 時(shí)間: 2025-3-31 13:26 作者: 簡(jiǎn)略 時(shí)間: 2025-3-31 21:33
In Vitro and In Vivo Systems to Study Tumor Dormancy and the Transition to Overt Metastases Inducedr even decades after the initial treatment of the primary tumor. This latency in the manifestation of the disease is attributed to the presence of early disseminated tumor cells (DTCs) that lay quiescent (dormant) for years until they emerge as clinically overt metastases. Given that to date we have作者: radiograph 時(shí)間: 2025-3-31 22:12
Modeling Tumor Cell Dormancy in an Ex Vivo Liver Metastatic Niche, aid in developing effective therapies to either maintain or eliminate these dormant cells and thus prevent them from emerging into overt metastatic disease. To study the behavior of dormant tumor cells—mechanisms that promote, maintain, and disrupt this state—we utilize the Legacy LiverChip?, an al作者: 技術(shù) 時(shí)間: 2025-4-1 01:59 作者: 休息 時(shí)間: 2025-4-1 09:31 作者: Handedness 時(shí)間: 2025-4-1 13:13
In Ovo Xenograft Model to Study Tumor Dormancy, Tumor Cell Dissemination and Metastasis,pitulates the complex tumor microenvironment. The chicken embryo chorioallantoic membrane (CAM) is an in vivo system suitable for the implantation of xenograft tumor models. It allows the study of different aspects of the metastatic process, including the dormancy-awakening transition. The main adva作者: 一條卷發(fā) 時(shí)間: 2025-4-1 17:20 作者: calamity 時(shí)間: 2025-4-1 19:15
Intracardiac Injection Mouse Model to Study Cancer Cell Dormancy in Brain Metastasis,of dormancy remains largely unknown and is still under intense investigation. Here, we outline a basic approach to generating and analyzing experimental mouse models to study dormant cancer cells in the brain. Cancer cells stably expressing EGFP and firefly luciferase are injected into the left vent
