標題: Titlebook: Crystallographic Methods and Protocols; Christopher Jones,Barbara Mulloy,Mark R. Sanderson Book 1996 Humana Press 1996 [打印本頁] 作者: affected 時間: 2025-3-21 20:07
書目名稱Crystallographic Methods and Protocols影響因子(影響力)
書目名稱Crystallographic Methods and Protocols影響因子(影響力)學科排名
書目名稱Crystallographic Methods and Protocols網(wǎng)絡(luò)公開度
書目名稱Crystallographic Methods and Protocols網(wǎng)絡(luò)公開度學科排名
書目名稱Crystallographic Methods and Protocols被引頻次
書目名稱Crystallographic Methods and Protocols被引頻次學科排名
書目名稱Crystallographic Methods and Protocols年度引用
書目名稱Crystallographic Methods and Protocols年度引用學科排名
書目名稱Crystallographic Methods and Protocols讀者反饋
書目名稱Crystallographic Methods and Protocols讀者反饋學科排名
作者: 假 時間: 2025-3-21 20:54
Overexpression, Isolation, and Crystallization of Proteins,ructural analysis by X-ray crystallography. High-level gene expression has facilitated the purification of many proteins that are normally only expressed at low concentrations, as well as those that have proven difficult to purify to homogeneity from natural sources. Furthermore, advances in oligonu作者: 冷淡一切 時間: 2025-3-22 03:11
Preliminary Characterization of Crystals,ded to answer many important questions about the crystals. Do they diffract X-rays? Do they diffract to a suitable resolution? Are they stable in the X-ray beam? What is their overall symmetry? What are the dimensions of their repeating unit? How many protein molecules are in that repeating unit? Th作者: sebaceous-gland 時間: 2025-3-22 06:21 作者: capsule 時間: 2025-3-22 11:04 作者: syring 時間: 2025-3-22 16:08
Structure Determination Using Isomorphous Replacement,udes and the calculation of phases for each diffraction point (maximum). Although amplitudes can be directly measured from diffracting crystals, phases are indirectly determined, because there are no lenses that can bend and focus X-rays. Thus, methods were developed to calculate phases from the int作者: syring 時間: 2025-3-22 19:24 作者: 磨坊 時間: 2025-3-22 22:18
Density Modification in X-Ray Crystallography,es measured experimentally and phases obtained with the multiple isomorphous replacement method. This method has poor precision, generating errors in the phases and therefore in the map. If the quality of the map is not sufficient to trace clearly a molecular model, it is necessary to improve the ph作者: 注視 時間: 2025-3-23 03:31
Refinement of Protein and Nucleic Acid Structures, and data collection; second, phase determination and calculation of electron density maps; and third, model building and refinement. This final part is necessary because the structural models arrived at after the first two steps are approximate and usually contain errors in the tracing of the macro作者: 簡略 時間: 2025-3-23 08:27
Recent Developments for Crystallographic Refinement of Macromolecules,ea detectors, and data analysis by high performance computers and new computational techniques. In addition, recombinant gene technology in many cases allows the expression of large amounts of protein. This has resulted in an unprecedented increase in the number of protein crystal structures elucida作者: maladorit 時間: 2025-3-23 10:03
The Crystallization and Structure Analysis of Oligonucleotide Sequences,53 and this date, there was considerable activity in refining the original B-form model of DNA and extending the approach to other polymorphs and a number of synthetic, repetitious polynucleotides, all of which were based on data from fiber-diffraction samples. These studies reached their zenith wit作者: 蠟燭 時間: 2025-3-23 16:32 作者: construct 時間: 2025-3-23 19:59
Virus Crystallography, resolution, and is thus an extraordinarily powerful tool in the pursuit of a fuller understanding of the functioning of these simple biological systems. We have already expanded our knowledge of virus evolution, assembly, antigenic variation, and host-cell interactions; further studies will no doub作者: Vasoconstrictor 時間: 2025-3-23 23:05 作者: GIST 時間: 2025-3-24 02:56 作者: 哀求 時間: 2025-3-24 09:31
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/240665.jpg作者: CURL 時間: 2025-3-24 11:51
the most mathematical section, at any rate, on a first reading. There is a bibliography at the end of this chapter that should provide further reading matter for readers at every level of crystallographic experience.作者: ascetic 時間: 2025-3-24 17:44
Annie Hau-Nung Chan,Lawrence Ka-Ki Hoructural analysis by X-ray crystallography. High-level gene expression has facilitated the purification of many proteins that are normally only expressed at low concentrations, as well as those that have proven difficult to purify to homogeneity from natural sources. Furthermore, advances in oligonu作者: 高談闊論 時間: 2025-3-24 21:47 作者: 蝕刻術(shù) 時間: 2025-3-24 23:15 作者: 難取悅 時間: 2025-3-25 04:13
Annie Hau-Nung Chan,Lawrence Ka-Ki Hodata generated by the interaction of X-rays with the crystals of the macromolecule under investigation. In general, there are two points at which the process languishes because of lack of necessary material or data. The first is the initial crystallization of the macromolecule; although work is bein作者: 干涉 時間: 2025-3-25 09:45
udes and the calculation of phases for each diffraction point (maximum). Although amplitudes can be directly measured from diffracting crystals, phases are indirectly determined, because there are no lenses that can bend and focus X-rays. Thus, methods were developed to calculate phases from the int作者: puzzle 時間: 2025-3-25 12:18 作者: 無法破譯 時間: 2025-3-25 17:51
Julia Brannen,Peter Moss,Ann Mooneyes measured experimentally and phases obtained with the multiple isomorphous replacement method. This method has poor precision, generating errors in the phases and therefore in the map. If the quality of the map is not sufficient to trace clearly a molecular model, it is necessary to improve the ph作者: 沉積物 時間: 2025-3-25 20:09 作者: nonsensical 時間: 2025-3-26 03:38
Julia Brannen,Peter Moss,Ann Mooneyea detectors, and data analysis by high performance computers and new computational techniques. In addition, recombinant gene technology in many cases allows the expression of large amounts of protein. This has resulted in an unprecedented increase in the number of protein crystal structures elucida作者: 阻撓 時間: 2025-3-26 05:08 作者: 含鐵 時間: 2025-3-26 09:43
The Foreign Policy Election That Wasn’tews . [.–.]). This has allowed a detailed understanding of how specific proteins interact with DNA (e.g., prokaryotic repressors), but has not facilitated the formulation of a common protein-DNA recognition code, which although suggested earlier (.), now appears to be too simplistic (.). However, so作者: myopia 時間: 2025-3-26 16:10
The Foreign Policy Election That Wasn’t resolution, and is thus an extraordinarily powerful tool in the pursuit of a fuller understanding of the functioning of these simple biological systems. We have already expanded our knowledge of virus evolution, assembly, antigenic variation, and host-cell interactions; further studies will no doub作者: Inordinate 時間: 2025-3-26 18:36
The Foreign Policy Election That Wasn’tst major breakthrough came with the crystallization (.) and X-ray crystallography (.,.) of the bacterial photosynthetic reaction center (. refs. . and . for reviews). The structure of another, entirely different membrane protein, the bacterial outer membrane porin from ., has now been determined by 作者: Inordinate 時間: 2025-3-26 21:07 作者: strain 時間: 2025-3-27 03:27 作者: 運動的我 時間: 2025-3-27 05:40 作者: GOUGE 時間: 2025-3-27 13:22 作者: OWL 時間: 2025-3-27 13:43
The Foreign Policy Election That Wasn’ts (.–.) have been obtained by this technique. Electron crystallography seems particularly appropriate for membrane proteins that are prone to form 2D crystals, and it is hoped that many more structures will be determined in this way.作者: 偏狂癥 時間: 2025-3-27 19:07
Modern Methods for Rapid X-Ray Diffraction Data Collection from Crystals of Macromolecules,in a glass or quartz capillary tube is usually held using plasticene and then aligned in the X-ray beam. This chapter will outline the principles of the most commonly used laboratory equipment, and give the basic steps involved in data collection and processing.作者: 群島 時間: 2025-3-28 01:39
Density Modification in X-Ray Crystallography,bio-polymer chain, and known noncrystallographic symmetry of the density distribution. To impose the physical constraints on an experimental map, an iterative algorithm has been proposed (.,.). It alternates real and reciprocal space operations, and merges gradually the physical constraints with the initial amplitudes and phases.作者: 有權(quán) 時間: 2025-3-28 02:33 作者: Vulvodynia 時間: 2025-3-28 08:57 作者: cardiovascular 時間: 2025-3-28 11:11
There may be more than one subunit in the crystallographic asymmetric unit. The similarity may be only partial so that a fragment is used. Examples include related structures, site-directed mutants, structures with ligands, and Fab-antigen complexes.作者: fallible 時間: 2025-3-28 16:24
ction intensities and those calculated improves. This improved agreement between observed and calculated structure factors leads to better phases and, concomitantly, to improved electron density maps. The refinement process is monitored by the conventional crystallographic R factor, defined by the equation: 作者: Mendacious 時間: 2025-3-28 21:09 作者: 無彈性 時間: 2025-3-29 00:12
Structure Determination Using Isomorphous Replacement,sful application to large biological molecules was undertaken in 1954 by Perutz and coworkers (.) while studying hemoglobin. Since then, this method has played a central role in the determination of almost all unique protein and nucleic acid structures, and it is likely to retain such a role in the foreseeable future.作者: 嚙齒動物 時間: 2025-3-29 03:53 作者: defibrillator 時間: 2025-3-29 09:20
Refinement of Protein and Nucleic Acid Structures,ction intensities and those calculated improves. This improved agreement between observed and calculated structure factors leads to better phases and, concomitantly, to improved electron density maps. The refinement process is monitored by the conventional crystallographic R factor, defined by the equation: 作者: orthodox 時間: 2025-3-29 15:07
The Crystallization and Structure Analysis of Oligonucleotide Sequences, relatively sparse para-crystalline diffraction data from ordered fibers. It is a tribute to the sophistication of these analyses, in spite of the inherent limitations of fiber data, that the refined “canonical” A- and B-DNA double helices are still major reference pomts for many studies (.).作者: PRO 時間: 2025-3-29 17:41 作者: Anterior 時間: 2025-3-29 20:23
1064-3745 , Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three- dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires cons作者: insidious 時間: 2025-3-30 01:13
The Foreign Policy Election That Wasn’tated the formulation of a common protein-DNA recognition code, which although suggested earlier (.), now appears to be too simplistic (.). However, some general observations have emerged from these recent protein-DNA crystal structures, some of which are discussed below.作者: 有惡臭 時間: 2025-3-30 08:08
Book 1996ographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three- dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires considerable r作者: 懶惰人民 時間: 2025-3-30 08:24
Overexpression, Isolation, and Crystallization of Proteins,rial expressed proteins can often be an advantage to the crystallographer where microheterogeneity presents a problem. Indeed, certain features or residues of a protein that are believed to impede crystal formation by preventing a close-packing arrangement may be successfully deleted by genetic mani作者: 指派 時間: 2025-3-30 16:04
Preliminary Characterization of Crystals,nd define clearly the side chains at such resolutions. Large repeating units (with any dimension >250 ?) and unstable crystals, on the other hand, complicate data acquisition from most laboratory equipment.作者: nonplus 時間: 2025-3-30 20:08 作者: 四海為家的人 時間: 2025-3-30 22:08 作者: jungle 時間: 2025-3-31 02:30 作者: countenance 時間: 2025-3-31 08:25
Annie Hau-Nung Chan,Lawrence Ka-Ki Horial expressed proteins can often be an advantage to the crystallographer where microheterogeneity presents a problem. Indeed, certain features or residues of a protein that are believed to impede crystal formation by preventing a close-packing arrangement may be successfully deleted by genetic mani
