標題: Titlebook: Corneal Regeneration; Methods and Protocol Mark Ahearne Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 limbal [打印本頁] 作者: 與生 時間: 2025-3-21 19:54
書目名稱Corneal Regeneration影響因子(影響力)
書目名稱Corneal Regeneration影響因子(影響力)學科排名
書目名稱Corneal Regeneration網絡公開度
書目名稱Corneal Regeneration網絡公開度學科排名
書目名稱Corneal Regeneration被引頻次
書目名稱Corneal Regeneration被引頻次學科排名
書目名稱Corneal Regeneration年度引用
書目名稱Corneal Regeneration年度引用學科排名
書目名稱Corneal Regeneration讀者反饋
書目名稱Corneal Regeneration讀者反饋學科排名
作者: 灌溉 時間: 2025-3-21 21:20
Some More Advanced STEM Problems,lume influence vector tropism and transduction efficiency. In this chapter, a detailed protocol for AAV vector production and specific approaches for AAV-mediated gene transfer to the cornea via subconjunctival and intrastromal injections are described.作者: 噴出 時間: 2025-3-22 00:30 作者: Annotate 時間: 2025-3-22 07:16 作者: BANAL 時間: 2025-3-22 08:59 作者: Mhc-Molecule 時間: 2025-3-22 14:15 作者: Mhc-Molecule 時間: 2025-3-22 19:44 作者: 敲竹杠 時間: 2025-3-22 22:54 作者: 半導體 時間: 2025-3-23 04:32 作者: AWE 時間: 2025-3-23 05:56 作者: harpsichord 時間: 2025-3-23 12:56
https://doi.org/10.1007/978-1-4614-3070-4ell (CEC) expansion..This chapter describes current methods that allow isolate and culture CECs. In brief, Descemet membrane is peeled out of the cornea and digested in order to obtain CECs. Cells are then seeded and cultured.作者: Pandemic 時間: 2025-3-23 14:19 作者: Gossamer 時間: 2025-3-23 19:27 作者: 異端邪說下 時間: 2025-3-23 23:16
https://doi.org/10.1007/978-1-4614-4415-2the cornea, as well as the axial and lateral arrangements of collagen molecules within the fibrils. Here we describe the typical experimental setup and considerations involved in the collection of X-ray scattering data from corneal tissue.作者: 培養(yǎng) 時間: 2025-3-24 02:22 作者: Curmudgeon 時間: 2025-3-24 09:42
Preparation of Dried Amniotic Membrane for Corneal Repair,e intellectual property associated with this methodology was patented by the University of Nottingham and licensed to NuVision. Biotherapies which formed the basis of the Tereo. manufacturing process which is used to manufacture Omnigen..作者: 鈍劍 時間: 2025-3-24 14:18
Development and Validation of a 3D In Vitro Model to Study the Chemotactic Behavior of Corneal Stroth platelet-derived growth factor-BB (PDGF-BB) in the inner section and corneal fibroblasts in the outer section. The cell migration toward the chemical stimuli over time can be monitored via confocal microscopy. The development of this in vitro model can be used for both qualitative and quantitative examinations of chemotaxis.作者: Epidural-Space 時間: 2025-3-24 16:26
X-Ray Diffraction Imaging of Corneal Ultrastructure,the cornea, as well as the axial and lateral arrangements of collagen molecules within the fibrils. Here we describe the typical experimental setup and considerations involved in the collection of X-ray scattering data from corneal tissue.作者: –DOX 時間: 2025-3-24 20:13
1064-3745 ation advice from the experts.This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for作者: Freeze 時間: 2025-3-24 23:49 作者: 支柱 時間: 2025-3-25 03:37 作者: Barter 時間: 2025-3-25 10:11 作者: 關心 時間: 2025-3-25 11:58
Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant, limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it is possible to obtain up to three limbal epithelial primary cultures from the same explant, thus increasing the efficiency of the in vitro cell culture.作者: 暫時過來 時間: 2025-3-25 19:22
A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture,scribe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for multiple downstream applications which are currently unattainable using traditional in vitro techniques.作者: 繼承人 時間: 2025-3-25 21:09 作者: 旁觀者 時間: 2025-3-26 03:05 作者: 衰弱的心 時間: 2025-3-26 05:18
Some More Advanced STEM Problems,face area, accessibility, immune privilege, avascularity, and ease of visualization. Herein, we describe the design, testing, and delivery of Cas9 and guide RNAs to target genes expressed in the cornea.作者: Bernstein-test 時間: 2025-3-26 11:28
Some More Advanced STEM Problems,iological processes, resulting in enhanced and accelerated extracellular matrix deposition. Herein, we describe the ex vivo formation of corneal stromal-like assemblies using human corneal fibroblasts and macromolecular crowding.作者: infinite 時間: 2025-3-26 13:43 作者: 漫步 時間: 2025-3-26 20:47
https://doi.org/10.1007/978-1-0716-0599-8limbal stem cell; Immunomodulation; collagen scaffolds; corneal repair; endothelial keratoplasty作者: 賠償 時間: 2025-3-26 21:07 作者: Substitution 時間: 2025-3-27 01:24 作者: CLOWN 時間: 2025-3-27 06:59
Mark AhearneIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 牙齒 時間: 2025-3-27 10:27
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/238270.jpg作者: braggadocio 時間: 2025-3-27 13:55
Dynamic Texturing in Virtual Environmentsresearch, and potentially in the development of clinically relevant cellular therapies. Postmortem corneal tissue obtained from otherwise discarded material after keratoplasties is oftentimes the source of the cells for ex vivo research. Relatively easy to isolate and cultivate as well as inexpensiv作者: Legion 時間: 2025-3-27 20:57 作者: Granular 時間: 2025-3-27 23:24
Tornadoes and Other Whirling Winds,e in vitro expanded cornea-limbal epithelial cell cultures are rich in stem cells for cornea, and hence can be used as a cell therapy for cornea-limbal deficiency. This chapter details the primary culture of these cornea-limbal cells, which can be used as model for further studies of the cornea surf作者: arousal 時間: 2025-3-28 04:31
Some More Advanced STEM Problems,ll deficiency. The protocols used to expand limbal epithelial cells in vitro need to be optimized, since the scarcity of human ocular tissue donors is limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it i作者: pessimism 時間: 2025-3-28 09:48
Some More Advanced STEM Problems,ated production of extracellular matrix, epithelial differentiation, and endothelial cell expansion to produce a highly transparent tissue. Here we describe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for mu作者: collagen 時間: 2025-3-28 13:34
Some More Advanced STEM Problems, an RNA-guided nuclease in which the sequence of the RNA can be altered to match the desired target. However, care must be taken in target choice and RNA guide design to ensure both maximum on-target and minimum off-target activity. The cornea is an ideal tissue for gene therapy due to its small sur作者: BRAND 時間: 2025-3-28 18:39 作者: Orgasm 時間: 2025-3-28 21:37
Tornadoes and Other Whirling Winds,. In addition to addressing the growing need for organ transplants, such tissue-engineered substitutes may also serve as good in vitro models for fundamental and preclinical studies. Recent progress in the field of corneal tissue engineering has led to the development of new technologies allowing th作者: 譏笑 時間: 2025-3-29 00:23
Some More Advanced STEM Problems,issue-specific extracellular matrix. However, in order to develop extracellular matrix-rich implantable devices, prolonged culture time is required in traditionally utilized dilute ex vivo microenvironments. Macromolecular crowding, by imitating the in vivo tissue density, dramatically accelerates b作者: ESPY 時間: 2025-3-29 03:29 作者: LIMIT 時間: 2025-3-29 09:01 作者: GEON 時間: 2025-3-29 14:13
Some More Advanced STEM Problems,generative medicine applications. Biomimetic corneas made have shown promise as replacements for human donor corneas, and have previously been fabricated from several different collagens or collagen-like peptides (CLPs). Prokaryotic expression systems allow for cheap, rapid, gram scale production of作者: 不能妥協 時間: 2025-3-29 18:23 作者: 蕨類 時間: 2025-3-29 21:17 作者: 糾纏 時間: 2025-3-30 01:24 作者: acrimony 時間: 2025-3-30 08:06
https://doi.org/10.1007/978-1-4614-4415-2h-intensity X-rays available at synchrotron radiation sources, coupled with minimal sample preparation requirements, facilitates the rapid generation of high-quality X-ray scattering data from corneal tissue at a close-to-physiological state of hydration. Analysis of resulting X-ray scatter patterns作者: Fibrin 時間: 2025-3-30 08:59
Corneal Regeneration978-1-0716-0599-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 怪物 時間: 2025-3-30 12:52 作者: 明確 時間: 2025-3-30 19:26
Isolation and Culture of Corneal Stromal Stem Cells,research, and potentially in the development of clinically relevant cellular therapies. Postmortem corneal tissue obtained from otherwise discarded material after keratoplasties is oftentimes the source of the cells for ex vivo research. Relatively easy to isolate and cultivate as well as inexpensiv作者: intuition 時間: 2025-3-30 21:39 作者: Unsaturated-Fat 時間: 2025-3-31 01:30
Primary Culture of Cornea-Limbal Epithelial Cells In Vitro,e in vitro expanded cornea-limbal epithelial cell cultures are rich in stem cells for cornea, and hence can be used as a cell therapy for cornea-limbal deficiency. This chapter details the primary culture of these cornea-limbal cells, which can be used as model for further studies of the cornea surf作者: ALIBI 時間: 2025-3-31 06:51
Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant,ll deficiency. The protocols used to expand limbal epithelial cells in vitro need to be optimized, since the scarcity of human ocular tissue donors is limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it i作者: 只有 時間: 2025-3-31 10:52
A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture,ated production of extracellular matrix, epithelial differentiation, and endothelial cell expansion to produce a highly transparent tissue. Here we describe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for mu作者: insolence 時間: 2025-3-31 14:43
Gene Editing for Corneal Stromal Regeneration, an RNA-guided nuclease in which the sequence of the RNA can be altered to match the desired target. However, care must be taken in target choice and RNA guide design to ensure both maximum on-target and minimum off-target activity. The cornea is an ideal tissue for gene therapy due to its small sur
